##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526360_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64424 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.062274928598036 39.0 37.0 40.0 33.0 40.0 2 36.41046814851608 39.0 37.0 40.0 30.0 40.0 3 35.947240158946975 39.0 36.0 40.0 28.0 40.0 4 36.212902024090404 39.0 37.0 39.0 30.0 40.0 5 36.44534645473737 39.0 38.0 40.0 30.0 40.0 6 36.337451881286476 39.0 38.0 40.0 30.0 40.0 7 36.54925183161555 39.0 38.0 40.0 31.0 40.0 8 36.230379982615176 39.0 36.0 40.0 30.0 40.0 9 36.257714516329315 39.0 36.0 39.0 31.0 40.0 10 36.09158077735005 39.0 36.0 40.0 30.0 40.0 11 35.25701601887496 38.0 35.0 39.0 29.0 40.0 12 33.01893704209611 38.0 31.0 39.0 17.0 40.0 13 32.48182354402086 37.0 30.0 39.0 15.0 40.0 14 31.857320253321742 37.0 29.0 39.0 13.0 40.0 15 32.42602135849994 37.0 30.0 39.0 16.0 40.0 16 32.40307959766547 37.0 30.0 39.0 16.0 40.0 17 32.473550229728055 36.0 30.0 39.0 17.0 40.0 18 32.708850738855084 36.0 30.0 39.0 19.0 40.0 19 33.54597665466286 37.0 31.0 39.0 24.0 40.0 20 33.58200360114243 37.0 31.0 39.0 24.0 40.0 21 34.883133614801935 38.0 35.0 39.0 26.0 40.0 22 34.65869862163169 38.0 34.0 39.0 25.0 40.0 23 34.81533279523159 38.0 35.0 39.0 26.0 40.0 24 34.374006581398234 38.0 33.0 39.0 24.0 40.0 25 34.58613249720601 38.0 34.0 39.0 25.0 40.0 26 34.823792375512234 38.0 35.0 39.0 27.0 40.0 27 34.755510368806654 38.0 35.0 39.0 26.0 40.0 28 34.59889171737241 38.0 35.0 39.0 26.0 40.0 29 34.48176145535825 38.0 35.0 39.0 25.0 40.0 30 34.275580528995405 38.0 34.0 39.0 24.0 40.0 31 33.95535825158326 38.0 33.0 39.0 23.0 40.0 32 32.85114243139203 38.0 32.0 39.0 15.0 40.0 33 34.12706444803179 38.0 34.0 39.0 24.0 40.0 34 33.7207872842419 38.0 33.0 39.0 22.0 40.0 35 33.83621010803427 38.0 33.0 39.0 23.0 40.0 36 31.959983856947723 35.0 31.0 38.0 17.0 39.0 37 31.674096609959022 35.0 31.0 38.0 12.0 39.0 38 31.00807152613933 35.0 30.0 38.0 5.0 39.0 39 29.150425307338878 34.0 26.0 38.0 4.0 39.0 40 24.992720104308955 30.0 11.0 37.0 4.0 39.0 41 21.953247237054512 26.0 9.0 33.0 4.0 37.0 42 25.62531044331305 30.0 20.0 34.0 4.0 37.0 43 21.62883397491618 26.0 8.0 33.0 4.0 37.0 44 22.689385943126783 27.0 9.0 33.0 4.0 37.0 45 25.19866819818701 30.0 19.0 34.0 4.0 37.0 46 24.961536073512978 30.0 12.0 36.0 4.0 38.0 47 24.303334161182168 30.0 8.0 35.0 4.0 38.0 48 24.22663914069291 29.0 9.0 35.0 4.0 38.0 49 21.771699987582267 26.0 4.0 35.0 4.0 38.0 50 24.05426549112132 29.0 4.0 35.0 4.0 38.0 51 25.504889482180555 32.0 4.0 38.0 4.0 39.0 52 25.396234322612692 32.0 4.0 38.0 4.0 39.0 53 24.96026325592947 31.0 4.0 37.0 4.0 39.0 54 24.049717496585124 31.0 4.0 37.0 4.0 39.0 55 24.282581025704705 31.0 4.0 37.0 4.0 39.0 56 23.481590711536075 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4960520387801353 1 2 2.3437993422541936 1 3 3.189163982257483 1 4 2.1478222496434682 1 5 2.4443926416478163 1 6 2.636930492597017 1 7 2.276559130711611 1 8 2.5099842049599985 1 9 2.7255891813096937 1 10 2.208152804562303 1 11 -2.38569876548058 1 12 -5.608005209901414 1 13 -20.89409075231325 1 14 -16.575928152783867 1 15 -9.463820113622493 1 16 -19.75020309513757 1 17 -20.019729907172135 1 18 -19.54675523258839 1 19 -4.969066550665431 1 20 -8.817143304289512 1 21 -6.178077851777193 1 22 -1.1476921378920935 1 23 -1.2005029530215054 1 24 -0.8595677261930206 1 25 -1.5652964381040135 1 26 2.9802097531712093 1 27 2.9571451048949484 1 28 3.252993919697701 1 29 2.946455310922822 1 30 2.965419027715349 1 31 1.6458127581932445 1 32 -10.215910002912505 1 33 1.7534509640807627 1 34 1.5951395286016563 1 35 1.937627493677411 1 36 3.894692981227383 1 37 3.612189937354568 1 38 4.530403251264044 1 39 6.508438093971304 1 40 8.080379395878168 1 41 -1.2694880841755882 1 42 -7.892124693063089 1 43 -12.472164339349886 1 44 -15.585567689429837 1 45 -13.065824709858683 1 46 -16.996246071150164 1 47 -17.260171455464395 1 48 -15.220557451495832 1 49 -12.599244379234559 1 50 -15.096954202161694 1 51 -16.761228881101882 1 52 -16.438088830297588 1 53 -16.819665108848305 1 54 -17.64951732243479 1 55 -16.541201844017273 1 56 -15.641851485884008 2 1 0.36481168880587234 2 2 1.9813014867043606 2 3 1.94418199563885 2 4 1.871102400612756 2 5 2.1625261122259616 2 6 2.050552040032258 2 7 2.2371012477128076 2 8 2.6244120656565144 2 9 2.1333778243016326 2 10 2.3623816602836953 2 11 -2.79640386069417 2 12 -10.734785103536684 2 13 -12.691826212941145 2 14 -14.603891782909104 2 15 -6.940574273855809 2 16 -2.358540717371234 2 17 -1.2497865206564391 2 18 -8.935844270159155 2 19 -1.3689636170576094 2 20 -2.3340415715737812 2 21 2.8557186827913412 2 22 2.830691804465083 2 23 2.026294096215068 2 24 2.3255412118752616 2 25 2.9757912270186466 2 26 2.793556810985585 2 27 2.98528029103322 2 28 3.0112714973267956 2 29 3.2456489976615472 2 30 2.579246442366234 2 31 -0.5998554524775344 2 32 -8.111089278946128 2 33 -1.1240580426099243 2 34 -0.5062500751039565 2 35 -0.4526624359846281 2 36 2.7205636279935526 2 37 2.8580297040383904 2 38 3.890156210605266 2 39 6.09447343450999 2 40 8.560050008333135 2 41 5.897264360497598 2 42 0.5676454218794404 2 43 -2.784910951118629 2 44 -10.177435934411825 2 45 -1.52182059251437 2 46 -12.018376796832099 2 47 -12.378716660473831 2 48 -8.813969700595162 2 49 -11.535082430358251 2 50 -8.147906338034057 2 51 -8.281386712633878 2 52 -7.582195881249724 2 53 -9.566963101642953 2 54 -14.762058066987887 2 55 -9.615485597662838 2 56 -9.261854917004268 3 1 2.1132517544873153 3 2 3.0312193848981224 3 3 3.304444721418818 3 4 3.036948049217031 3 5 3.2397018100913115 3 6 2.976660414416493 3 7 2.8906316253811184 3 8 3.252983494227948 3 9 3.0161364449912895 3 10 2.83857213647417 3 11 1.908686451292695 3 12 2.585740348023247 3 13 -3.692811434616022 3 14 -4.94280803906674 3 15 -1.4305578534945624 3 16 4.379718724336481 3 17 3.6057143003616225 3 18 3.484516977788303 3 19 2.2651907343381197 3 20 2.1414920901667784 3 21 3.723698978357845 3 22 4.315420868504496 3 23 4.124159449252836 3 24 3.9147037734516132 3 25 4.031292048036711 3 26 3.596840883235174 3 27 3.4414379265012016 3 28 3.5419775728604534 3 29 3.7241383244267325 3 30 3.602234948113356 3 31 4.2438227084420035 3 32 3.047203003484082 3 33 3.9196201183096164 3 34 4.346951895339394 3 35 4.3089139186951755 3 36 6.082468389898313 3 37 6.2744500652863415 3 38 6.202471481541227 3 39 8.65769182531459 3 40 10.547898941009649 3 41 9.80826600253372 3 42 7.784065783127389 3 43 3.6173878994560837 3 44 4.692186397279471 3 45 8.174402724398604 3 46 2.181294795942943 3 47 -2.668700240112585 3 48 2.2957183125411262 3 49 -7.524901806384523 3 50 2.4028325782220996 3 51 0.5856083612577478 3 52 1.4017121647272859 3 53 0.6629219558283097 3 54 -1.815260037431237 3 55 0.8475685895292813 3 56 3.646847874457144 4 1 1.8166659503543414 4 2 2.915046878159572 4 3 3.408398000843796 4 4 2.695977698812719 4 5 3.1305373896061397 4 6 3.352221959789361 4 7 3.038879603817776 4 8 3.033217097103055 4 9 3.5249414764378244 4 10 3.0762181382711162 4 11 3.16358312694409 4 12 5.26597395089836 4 13 5.7489675411252215 4 14 4.403373452397773 4 15 6.878337025211639 4 16 6.626169757579969 4 17 5.997448352473032 4 18 6.187122548318399 4 19 5.054588757698411 4 20 4.846613383967316 4 21 3.9095031113317873 4 22 3.7903534830597536 4 23 3.4388943998368404 4 24 3.680561814781356 4 25 4.160133018387114 4 26 2.92487322824865 4 27 3.007835051388895 4 28 3.098671193705023 4 29 3.166815772764558 4 30 3.445002243710846 4 31 4.160174909699862 4 32 4.552324297284624 4 33 3.676852822712128 4 34 4.426736261916211 4 35 4.074771960024918 4 36 4.755154822962101 4 37 5.382536318655614 4 38 5.979290888549311 4 39 8.063352202673084 4 40 10.021663541189348 4 41 5.448337880341448 4 42 7.126743231465213 4 43 5.504180352286273 4 44 10.198296010936257 4 45 8.056253577947569 4 46 7.832956970246627 4 47 8.704345581989841 4 48 7.7179105856767976 4 49 5.428916702150964 4 50 7.948385138869 4 51 6.870424174375447 4 52 6.972781347117223 4 53 6.206947113061016 4 54 1.7137606274384858 4 55 5.755475148379237 4 56 5.129058117044746 5 1 2.109499950735511 5 2 2.959357913036648 5 3 3.7073307243048177 5 4 3.01530302757201 5 5 3.375343945733448 5 6 3.099315537071618 5 7 3.32208905683855 5 8 3.3415837828282307 5 9 3.3131061419609864 5 10 3.1603614582634876 5 11 3.7863199289261757 5 12 6.609694171977072 5 13 7.115557973753386 5 14 6.893844197585494 5 15 7.231491208554498 5 16 7.0186797632975235 5 17 5.967907662554989 5 18 6.607172100443421 5 19 4.5575428266902165 5 20 5.187379536054223 5 21 4.055300709959575 5 22 4.456257809341437 5 23 3.698185423278808 5 24 4.146161204909049 5 25 4.102576319320981 5 26 3.358168444562736 5 27 3.358321043384315 5 28 3.6020064328893184 5 29 3.3853215856099936 5 30 3.1173864632648716 5 31 3.9529136175329143 5 32 4.741575297856375 5 33 3.9640645627540607 5 34 4.097024045411544 5 35 4.217139026920286 5 36 5.124604032033954 5 37 5.35785554869182 5 38 6.121086200155947 5 39 8.223348390971157 5 40 8.029572822683534 5 41 3.5346729289406476 5 42 9.330097529159133 5 43 13.1325394146076 5 44 13.36000746510054 5 45 10.70109825109413 5 46 11.29788931253746 5 47 10.793348821936476 5 48 11.714765931588744 5 49 11.913193431710713 5 50 11.150595925985446 5 51 10.099316874966263 5 52 10.460298023313147 5 53 10.38009367300003 5 54 10.978102155930955 5 55 12.035158777119467 5 56 10.276285103894374 6 1 2.4224092636448233 6 2 3.3213292750080115 6 3 3.686129703103802 6 4 3.454530466799447 6 5 3.2397018100913115 6 6 3.3795908173468945 6 7 3.582939317688812 6 8 3.3093937506382005 6 9 3.5099093387641815 6 10 3.303773601675637 6 11 4.169492312098555 6 12 7.163029725312629 6 13 6.6852105434059546 6 14 7.950232253973553 6 15 7.455156432219727 6 16 7.44858319320095 6 17 7.283369978017301 6 18 7.114553607824931 6 19 5.424897694045079 6 20 5.730503079177765 6 21 4.781574436233306 6 22 4.581354934438558 6 23 3.8157345408279255 6 24 4.183568242316085 6 25 3.776346993091657 6 26 3.5880496744439654 6 27 3.90810459316787 6 28 3.2547980856809673 6 29 3.6699258702142785 6 30 3.2432605891389983 6 31 3.4540791186984166 6 32 5.348301904582986 6 33 3.6807922794817785 6 34 4.254644203031702 6 35 3.039316849098107 6 36 3.123494030923954 6 37 2.2260984169346933 6 38 3.3365374156071574 6 39 5.65769182531459 6 40 1.3273861204968327 6 41 1.1833575776252978 6 42 5.734248933310539 6 43 10.826179108247292 6 44 9.273871378964454 6 45 5.035208585204462 6 46 8.949792964441112 6 47 8.33569536428302 6 48 8.147000363823178 6 49 9.964475482992764 6 50 8.78085455624408 6 51 8.204655980305365 6 52 8.671309234324358 6 53 9.505412798319153 6 54 8.557633735462534 6 55 7.481268223228913 6 56 8.849045676654946 7 1 2.3834898497254144 7 2 3.47735033727907 7 3 3.875575673799773 7 4 3.602424239693221 7 5 3.7724399053294064 7 6 3.879934223940303 7 7 3.6257506730001694 7 8 3.728578732323186 7 9 3.678636444991284 7 10 3.6436316602836953 7 11 4.344400737006978 7 12 6.971752252785155 7 13 7.271176660622071 7 14 8.01284672283802 7 15 7.947715956029246 7 16 7.680611581479344 7 17 7.223273824171148 7 18 6.200290787312113 7 19 5.660131210528597 7 20 5.519765899690583 7 21 4.7626870735959415 7 22 4.772861344694974 7 23 4.715230877824261 7 24 4.231072821070661 7 25 3.9384349051795695 7 26 3.5974361213304107 7 27 3.9170331645964396 7 28 4.5202513823842665 7 29 4.127114514902921 7 30 4.206401614780027 7 31 4.231322708442001 7 32 5.9650601463412265 7 33 3.842536784976282 7 34 5.002011419148914 7 35 4.281532966314224 7 36 3.3143136279935526 7 37 2.887842922429197 7 38 4.305745291065033 7 39 6.3972751586479255 7 40 -0.5872201066093972 7 41 -9.312864949847231 7 42 4.774541973603579 7 43 10.971554566122752 7 44 9.05855544489852 7 45 6.931843200589078 7 46 9.184568605466751 7 47 10.832490236077895 7 48 9.818932598255412 7 49 13.038193431710717 7 50 10.283487340126864 7 51 11.130251218400602 7 52 11.382962164727285 7 53 11.350421955828313 7 54 12.354680438759239 7 55 10.721080494291183 7 56 11.011133588742858 8 1 2.0622532905856303 8 2 3.0825250684618695 8 3 4.000911695305149 8 4 2.7005425192631094 8 5 2.926337754791774 8 6 3.098012180929551 8 7 2.723196909559306 8 8 2.839465829097378 8 9 2.874200961120316 8 10 2.5881881118965993 8 11 3.347088909049994 8 12 5.217383973215259 8 13 6.587372897181215 8 14 6.40834403466598 8 15 6.570363805491613 8 16 6.492775559973968 8 17 6.204792641375452 8 18 6.498341862580929 8 19 4.995144651388813 8 20 5.3359621362497265 8 21 3.4727005144561574 8 22 3.5937618823293818 8 23 3.6688599100823254 8 24 4.095656154403997 8 25 3.6561768406634414 8 26 2.6370866589648188 8 27 3.0709310140588073 8 28 2.562254070556307 8 29 2.518915590171737 8 30 2.39793387284454 8 31 3.0760807729581288 8 32 4.09543649042725 8 33 2.3905878602450983 8 34 2.546030236353218 8 35 2.9844899555615356 8 36 1.7958324451978491 8 37 0.3982595890958649 8 38 1.6958662588069728 8 39 2.335111180153298 8 40 -0.21154806359864153 8 41 -8.989276240169811 8 42 4.258412941345515 8 43 8.402334136015224 8 44 6.683891466403896 8 45 3.0591953511267143 8 46 7.203721831273203 8 47 7.121468730701547 8 48 6.736271307932832 8 49 9.698139668269853 8 50 7.274414759481701 8 51 8.550278100121034 8 52 8.612464852899329 8 53 8.770448837548741 8 54 9.465231514028055 8 55 9.91362081687183 8 56 9.86866047046329 9 1 1.7973787386142988 9 2 2.816933670612407 9 3 3.5012701182442143 9 4 2.8718686841376595 9 5 3.046051016440522 9 6 3.052850890606969 9 7 2.8604728952223866 9 8 3.157745398989853 9 9 3.3161364449912867 9 10 2.6512705491725796 9 11 3.9499562925625398 9 12 6.457168919451821 9 13 6.58020443839985 9 14 7.374652278393576 9 15 7.02341040047369 9 16 7.014639359257121 9 17 5.885079379726701 9 18 6.638485231756555 9 19 4.874714543861927 9 20 5.0176825663572515 9 21 3.8967148513737158 9 22 3.3138335669171965 9 23 3.286064211157594 9 24 4.340100598848444 9 25 3.477323794068461 9 26 2.957158343552635 9 27 2.9350887201519953 9 28 2.5515013823842665 9 29 3.370170070458478 9 30 2.546679392557806 9 31 4.377156041775336 9 32 5.056726813007895 9 33 3.686286784976282 9 34 3.5929836413711413 9 35 3.4120885218697836 9 36 3.0427858502157754 9 37 3.7760373668736413 9 38 4.123106402176145 9 39 5.902136269759037 9 40 1.2002798933906043 9 41 -1.6774482831805635 9 42 6.352319751381355 9 43 11.939610121678307 9 44 10.03980544489852 9 45 7.661704311700191 9 46 9.768596383244532 9 47 9.618601347189003 9 48 10.581432598255411 9 49 11.535415653932937 9 50 10.064737340126861 9 51 10.564973440622829 9 52 10.446156609171734 9 53 10.062921955828312 9 54 9.749819327648126 9 55 10.68566382762452 9 56 9.061133588742859 10 1 2.4251565163920787 10 2 3.5351154887942258 10 3 3.698492340466437 10 4 3.189545451814432 10 5 3.543273238662742 10 6 3.411184223940303 10 7 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2 2.291007744686482 115 3 2.9901590071331015 115 4 2.5052020174709995 115 5 2.4460510164405207 115 6 2.3232212609773413 115 7 1.8641765989260932 115 8 2.2355231767676287 115 9 2.097617926472765 115 10 1.9920112899133215 115 11 0.40921555182179503 115 12 -2.5132014509185474 115 13 0.3579822161776285 115 14 -5.932755129013831 115 15 -1.058071081007789 115 16 -10.607582862965103 115 17 -7.574179879532554 115 18 -0.6689221756508523 115 19 -0.9289891598417732 115 20 -0.8934285447538599 115 21 1.9744926291514986 115 22 1.7693891224727523 115 23 2.2453234704168565 115 24 2.8178783766262185 115 25 1.7069534236980886 115 26 1.7756768620711512 115 27 2.9424961275594015 115 28 2.9959458268287094 115 29 2.4812811815695923 115 30 2.639271985150394 115 31 2.210489375108672 115 32 -3.3914213351402545 115 33 1.7973978960873964 115 34 2.1189095672970666 115 35 2.0824588922401546 115 36 3.6464895539194764 115 37 3.9908521816884566 115 38 4.656439735509483 115 39 7.083617751240514 115 40 8.841020634131347 115 41 8.563292457560177 115 42 4.200467899529503 115 43 1.8951656772338623 115 44 -2.0676019625088884 115 45 3.5209635709594487 115 46 -1.427699913051768 115 47 -6.422139393551737 115 48 -5.21115999433718 115 49 -7.938658420141138 115 50 -1.7871145117249903 115 51 1.0168252924746781 115 52 -0.08347302045789817 115 53 0.9777367706431264 115 54 -0.016847339018539742 115 55 0.1782564202171102 115 56 2.6500224776317474 116 1 2.1751565163920787 116 2 2.909241362920099 116 3 3.496569263543357 116 4 2.951783214052192 116 5 2.8221193925088954 116 6 3.1515688393249164 116 7 2.835686570436067 116 8 3.2846684759129303 116 9 2.757162086016926 116 10 3.0675098654118997 116 11 0.7354263780326207 116 12 -1.1171900549071516 116 13 -3.595009236813823 116 14 -7.882185328444027 116 15 -3.6877007106374187 116 16 -7.746471751853992 116 17 -9.101245406598082 116 18 -2.116215622944299 116 19 -3.2316957125483263 116 20 -2.673343074668388 116 21 2.438167842826708 116 22 2.2544318575154847 116 23 2.60144882654221 116 24 2.595656154403997 116 25 2.5538195205641827 116 26 3.84903868543298 116 27 4.171840856904133 116 28 3.7245783054611863 116 29 3.12871707900549 116 30 3.4355682814466917 116 31 2.8194637340830297 116 32 -5.871478315197233 116 33 3.782440631130129 116 34 3.7079409063284032 116 35 2.2398662996475593 116 36 5.348768756198677 116 37 5.577746768583044 116 38 5.891482470552216 116 39 7.33076874839151 116 40 10.324638867749577 116 41 7.675115819383539 116 42 4.223259922321528 116 43 -9.53645825439007 116 44 -0.21788686279378666 116 45 4.265977815973695 116 46 -4.530976266328121 116 47 -7.798920020332364 116 48 -6.413439196616382 116 49 -5.247062978545696 116 50 -2.4224421470526245 116 51 -0.012376986727602457 116 52 0.4158147288298508 116 53 -2.593488300581946 116 54 -4.835223407394608 116 55 -1.8374130954524048 116 56 0.06882589643516468 117 1 0.2206110618466255 117 2 1.4139033675821011 117 3 1.8386438556179527 117 4 1.469848482117463 117 5 1.4258489962385 117 6 0.9073963451524278 117 7 0.9079476426971382 117 8 1.321381762626217 117 9 0.7676515965064397 117 10 0.8775331754352109 117 11 -8.64802350541726 117 12 -13.248891686608783 117 13 -9.773330915135503 117 14 -11.520297216555917 117 15 -13.142246165182872 117 16 -12.102532357914598 117 17 -6.264415569768246 117 18 -1.5847470914757693 117 19 -2.2404369712895864 117 20 -2.2671659184912336 117 21 2.509846164505035 117 22 2.5976719507555757 117 23 2.516367241460628 117 24 0.0956561544039971 117 25 2.173283390028054 117 26 2.8228149092091996 117 27 2.8734725585358305 117 28 1.6727135035963911 117 29 2.2237054239938345 117 30 3.026477372355785 117 31 2.528671193290485 117 32 -8.106909550628469 117 33 1.8529534516429464 117 34 2.652579600967101 117 35 2.217139026920286 117 36 4.629654537084459 117 37 4.8225020133382905 117 38 4.666540745610494 117 39 7.2031463707691366 117 40 7.282098075208786 117 41 8.110430504698222 117 42 5.501814700876306 117 43 -3.261399979331795 117 44 2.370108475201551 117 45 2.4081689581648362 117 46 -3.0455450308968857 117 47 -4.822812794225136 117 48 -8.770082553259739 117 49 -3.4807459622286796 117 50 -1.9504141750246546 117 51 -1.4663396906903046 117 52 -1.155863592848469 117 53 -2.8825325896262335 117 54 -6.526948349119548 117 55 -2.096154354193665 117 56 0.5187093463186159 118 1 0.9751565163920759 118 2 2.096933670612408 118 3 2.763492340466435 118 4 2.574090906359885 118 5 2.2082732386627413 118 6 2.1661842239403057 118 7 2.204917339666835 118 8 2.3177453989898495 118 9 2.309469778324619 118 10 1.5690483269503588 118 11 -5.752265929659686 118 12 -9.929497747214846 118 13 -9.784240006044595 118 14 -0.14756994382864264 118 15 -15.387700710637418 118 16 -5.316471751853992 118 17 -8.850476175828852 118 18 -1.2192925460212223 118 19 -2.9186187894714024 118 20 -2.795650766976081 118 21 2.298937073595944 118 22 2.2782780113616354 118 23 1.8460642111575964 118 24 0.03565615440399483 118 25 2.028434905179573 118 26 3.1882694546637467 118 27 2.7595331645964407 118 28 2.1115013823842688 118 29 2.527947848236259 118 30 2.8755682814466894 118 31 0.5571560417753361 118 32 -5.809939853658772 118 33 1.6262867849762799 118 34 1.844094752482249 118 35 2.2898662996475565 118 36 4.527230294660217 118 37 4.8315929224291985 118 38 5.465328624398371 118 39 7.5776918253145915 118 40 9.606946560057274 118 41 8.75588505015277 118 42 2.50787530693691 118 43 1.817387899456083 118 44 2.0998054448985215 118 45 3.25059320058908 118 46 -5.8425147278665825 118 47 -6.085843097255442 118 48 -7.97190073507792 118 49 -5.2801399016226185 118 50 -4.64859599320647 118 51 -4.743915448266062 118 52 -5.324954501939381 118 53 -5.890411377505021 118 54 -10.43906956124076 118 55 -6.614336172375481 118 56 -4.2588664112571415 119 1 0.845888223709153 119 2 2.1559580608563067 119 3 2.3722728282713135 119 4 2.1136031014818357 119 5 1.9853464093944524 119 6 2.200818370281766 119 7 1.921990510398544 119 8 2.104086862404486 119 9 1.9738600222270577 119 10 1.5412434489015823 119 11 -8.46250983209871 119 12 -8.43144896672704 119 13 0.013808774443212712 119 14 -2.2963504316335204 119 15 -5.1755055886862 119 16 -7.984276629902773 119 17 -11.16315910265812 119 18 -2.185634009435855 119 19 -1.4244724480079896 119 20 -1.066870279171205 119 21 0.15064439066911461 119 22 0.5119365479470019 119 23 0.21777152823077017 119 24 -4.562880430961858 119 25 0.3957519783503045 119 26 2.2219279912491103 119 27 1.820996579230588 119 28 2.1124769921403654 119 29 2.7942893116508927 119 30 2.2038609643735185 119 31 0.18203409055582398 119 32 -7.800183756097798 119 33 0.3814087361957945 119 34 1.3406801183359107 119 35 1.880110202086584 119 36 4.923815660513878 119 37 4.414519751697487 119 38 4.8867920390325175 119 39 7.65769182531459 119 40 9.132800218593857 119 41 7.460763098933256 119 42 4.083485063034473 119 43 2.9832415579926703 119 44 3.1202932497765694 119 45 3.3091297859549336 119 46 -2.644953752256825 119 47 -5.052672365548123 119 48 -8.195803174102311 119 49 -4.5908716089396915 119 50 -1.9881081883284217 119 51 -4.101964228753868 119 52 -4.918613038524747 119 53 -8.792362597017217 119 54 -8.226874439289542 119 55 -7.987506904082798 119 56 -5.824232264915679 120 1 0.5262203461793149 120 2 1.5254443089102807 120 3 2.3022157447217566 120 4 1.5962185659343504 120 5 1.966145579088277 120 6 1.4457586920254073 120 7 0.9878960630710907 120 8 1.106681569202614 120 9 0.8218102038565362 120 10 1.7524525822695054 120 11 -10.859499972212877 120 12 -1.479710513172293 120 13 -7.75785702732119 120 14 -8.885867816169071 120 15 -12.794083689360821 120 16 -11.43264196461995 120 17 -8.849199580084171 120 18 -3.273335099212712 120 19 -3.0373421937267224 120 20 -4.498629490380338 120 21 0.12106473317040667 120 22 0.6370014156169574 120 23 0.5498939983916387 120 24 -6.691577888149194 120 25 -0.1413523288629861 120 26 1.7938013695573645 120 27 2.117831036936863 120 28 1.9983098930225651 120 29 0.584118061002215 120 30 1.5738661537871153 120 31 -4.346248213543813 120 32 -9.920578151531114 120 33 -3.813713215023718 120 34 -10.323564821985837 120 35 -3.3399209343949927 120 36 -2.782982471297231 120 37 -2.523726226506973 120 38 -2.284458609644183 120 39 -1.3635847704300907 120 40 -2.360713014410816 120 41 1.2729063267485117 120 42 -2.9985076717864914 120 43 -4.063463164373703 120 44 -4.787854129569565 120 45 -9.434513182389646 120 46 -10.763791323611262 120 47 -12.062013310021397 120 48 -10.015730522311964 120 49 -14.526522880346024 120 50 -8.938808759163917 120 51 -8.798383533372446 120 52 -8.192614076407464 120 53 -11.074666696653958 120 54 -10.18587807187906 120 55 -10.978165959609525 120 56 -7.813334496363526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1499.0 5 240.0 6 15.0 7 121.0 8 198.0 9 159.0 10 114.0 11 105.0 12 95.0 13 173.0 14 207.0 15 262.0 16 355.0 17 414.0 18 511.0 19 649.0 20 756.0 21 942.0 22 1175.0 23 1327.0 24 1585.0 25 1785.0 26 1821.0 27 1947.0 28 2136.0 29 2267.0 30 2386.0 31 2910.0 32 3504.0 33 4432.0 34 5084.0 35 6044.0 36 7103.0 37 6910.0 38 4103.0 39 1090.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.45051533589967 0.4020240904010927 0.12572954178566995 0.02173103191357258 8 99.27666708059108 0.5262014156215076 0.12262510865515958 0.07450639513224885 9 96.98489341551647 1.7730441397941281 0.6350044248474592 0.6070580198419475 10 54.46883149136967 26.43580032286105 8.549608841425556 10.545759344343724 11 35.56959470996383 24.961582043680053 17.880260155534515 21.5885630908216 12 32.28455234074258 13.347510244629332 23.426052402831242 30.941885011796842 13 27.649633676890602 13.994784552340741 23.205637650565006 35.14994412020365 14 26.36890068774937 12.711719683914739 30.394485585207953 30.524894043127944 15 30.4695397919032 14.457906278675523 26.823957571892937 28.248596357528342 16 33.583352744778324 19.00923984781427 21.153816290084634 26.25359111732277 17 30.58302926791398 18.262557254871517 23.186088036643117 27.968325440571384 18 30.92362620304253 20.742005588326606 26.544551381558524 21.78981682707234 19 36.20119537374835 22.525809205930294 18.369944888612903 22.903050531708452 20 32.025463861501436 25.800791863985715 20.987500970421554 21.1862433040913 21 31.24427540169215 19.512535900023288 27.12566948692075 22.117519211363813 22 29.290138308936527 20.490212818801325 25.080330947982798 25.139317924279347 23 29.718738358375763 20.971998013162796 24.337203526636035 24.97206010182541 24 28.829318266484538 22.328635291195827 23.669750403576305 25.172296038743326 25 31.99584005960512 21.374022103563888 21.02477337638147 25.605364460449522 26 25.008943430855613 21.145381301230305 23.484671737203115 30.36100353071096 27 24.06832298136646 18.419254658385093 26.819875776397517 30.692546583850934 28 23.59234321790107 24.122595593151814 24.923416629087686 27.36164455985943 29 29.50939295140506 22.89396056512964 23.556901102313304 24.039745381151995 30 27.656567852539716 19.530412907433575 29.759150270975354 23.053868969051354 31 27.271312763540745 22.13508845633198 27.570057103735863 23.023541676391417 32 33.38508111464721 17.182333307459444 25.46146083986649 23.97112473802686 33 31.172124134712607 19.17087967644085 27.45119390215447 22.205802286692077 34 24.724250427217648 23.368028584744447 32.832064626378745 19.075656361659156 35 24.555082771686802 25.381246700003107 32.27008727521198 17.793583253098113 36 27.065190345857175 27.11495396864892 25.957949738740982 19.861905946752923 37 26.259670314275485 24.942795324004173 28.557196893046715 20.24033746867363 38 29.81812529146588 24.51111456552153 26.09047100886056 19.58028913415203 39 23.736791247155637 18.64966802780462 24.199682054798792 33.413858670240955 40 17.146650007808837 13.891925659846946 22.8486646884273 46.11275964391691 41 16.940737956524462 16.79665476414208 25.255277829981832 41.00732944935162 42 24.9428344766438 21.416460559677695 30.73716303451709 22.903541929161417 43 14.332658464413642 12.206821367388256 25.905622177230963 47.554897990967135 44 16.57360326917678 13.762984683532459 22.580091711638644 47.08332033565212 45 24.722226538826124 19.799226119252225 31.460272567636867 24.01827477428478 46 22.522368584565676 22.074996893252145 29.344786877097057 26.057847645085126 47 19.835390946502056 22.79990682506406 29.899836943862102 27.464865284571783 48 24.168400782681616 25.69183464297916 30.109948131813525 20.029816442525703 49 17.501790663635514 15.561645542026097 21.11893120737442 45.81763258696397 50 24.181654116622067 23.097268031613666 30.166780757981204 22.55429709378306 51 22.62119890329013 26.688683948155536 29.59870388833499 21.09141326021934 52 23.333904669897315 23.883945961948985 31.3274225968805 21.454726771273197 53 23.0422202171681 21.19566908310258 32.70900096450017 23.053109735229146 54 19.71960147349115 21.350078492935637 27.62329608157048 31.307023952002737 55 23.850543436008273 23.64374232270303 29.22270769517827 23.28300654611043 56 24.72977806808815 23.428047092489773 27.57119084278138 24.270983996640695 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 2.0 11 3.0 12 12.0 13 28.5 14 36.0 15 61.5 16 87.0 17 102.5 18 118.0 19 133.0 20 148.0 21 185.0 22 263.0 23 304.0 24 383.5 25 463.0 26 579.0 27 695.0 28 839.0 29 1077.0 30 1171.0 31 1245.5 32 1320.0 33 1280.5 34 1241.0 35 1170.5 36 1100.0 37 1066.5 38 950.0 39 867.0 40 910.5 41 954.0 42 1035.0 43 1116.0 44 1163.5 45 1211.0 46 1467.0 47 1940.5 48 2158.0 49 2101.5 50 2045.0 51 2145.5 52 2246.0 53 2415.0 54 2869.0 55 3154.0 56 3411.0 57 3668.0 58 4085.0 59 4502.0 60 4962.0 61 5422.0 62 5278.0 63 4791.0 64 4448.0 65 4261.5 66 4075.0 67 3641.5 68 3208.0 69 2789.5 70 2371.0 71 2048.0 72 1469.5 73 1214.0 74 993.0 75 772.0 76 615.0 77 458.0 78 343.5 79 177.5 80 126.0 81 84.5 82 43.0 83 31.0 84 19.0 85 11.5 86 4.0 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02328324847882777 10 0.0 11 0.0015522165652551844 12 0.0 13 0.0 14 0.01707438221780703 15 0.1971315037874084 16 0.04501428039240035 17 0.029492114739848505 18 0.0062088662610207375 19 0.013969949087296658 20 0.029492114739848505 21 0.013969949087296658 22 0.004656649695765553 23 0.0 24 0.0 25 0.0 26 0.20334037004842917 27 0.03725319756612443 28 0.1785049050043462 29 0.02173103191357258 30 0.041909847261889975 31 0.24059356761455358 32 0.013969949087296658 33 0.21575810257047062 34 0.08381969452377995 35 0.04656649695765554 36 0.18626598783062215 37 0.28095119831118837 38 0.14590835713398734 39 0.40668074009685834 40 0.6115733267105427 41 0.8878678753259656 42 0.21265366943996025 43 0.33217434496460946 44 0.48118713522910717 45 0.11331180926362845 46 0.07450639513224885 47 0.04501428039240035 48 0.04656649695765554 49 0.31354774618154724 50 0.2297280516577673 51 0.3601142431392028 52 0.38339749161803055 53 0.2204147522662362 54 0.13504284117720106 55 0.17229603874332547 56 0.19402707065689803 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 64424.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.04594561033156 #Duplication Level Percentage of deduplicated Percentage of total 1 93.16116079576565 79.22979014032038 2 4.4752692097098015 7.612070036011424 3 1.1279430553020624 2.8778095119831115 4 0.44168643913122835 1.5025456351670186 5 0.2463953276145282 1.0477461815472495 6 0.13688629311918232 0.698497454364833 7 0.09125752874612156 0.5432757978393145 8 0.04197846322321591 0.28560784800695393 9 0.043803613798138345 0.3352787780951198 >10 0.20624201496623473 3.205327207251956 >50 0.016426355174301882 0.9778964361107663 >100 0.010950903449534586 1.684154973301875 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 240 0.3725319756612443 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 214 0.33217434496460946 No Hit AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 202 0.31354774618154724 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 175 0.27163789891965723 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 131 0.20334037004842917 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 123 0.1909226375263877 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 89 0.1381472743077114 No Hit AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC 87 0.13504284117720106 No Hit AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA 83 0.1288339749161803 No Hit AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCA 80 0.12417732522041476 No Hit AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 67 0.10399850987209734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0031044331305103688 0.0 0.0 0.0 0.0 11 0.0062088662610207375 0.0 0.0 0.0 0.0 12 0.007761082826275922 0.0 0.0 0.0 0.0 13 0.007761082826275922 0.0 0.0 0.0 0.0 14 0.009313299391531107 0.0 0.0 0.0 0.0 15 0.009313299391531107 0.0 0.0 0.0 0.0 16 0.01396994908729666 0.0 0.0 0.0 0.0 17 0.015522165652551843 0.0 0.0 0.0 0.0 18 0.018626598783062213 0.0 0.0 0.0 0.0 19 0.0201788153483174 0.0 0.0 0.0 0.0 20 0.0201788153483174 0.0 0.0 0.0 0.0 21 0.02173103191357258 0.0 0.0 0.0 0.0 22 0.023283248478827765 0.0 0.0 0.0 0.0 23 0.02483546504408295 0.0 0.0 0.0 0.0 24 0.026387681609338135 0.0 0.0 0.0 0.0 25 0.031044331305103687 0.0 0.0 0.0 0.0 26 0.031044331305103687 0.0 0.0 0.0 0.0 27 0.03570098100086924 0.0 0.0 0.0 0.0 28 0.03880541413137961 0.0 0.0 0.0 0.0 29 0.03880541413137961 0.0 0.0 0.0 0.0 30 0.03880541413137961 0.0 0.0 0.0 0.0 31 0.03880541413137961 0.0 0.0 0.0 0.0 32 0.03880541413137961 0.0 0.0 0.0 0.0 33 0.03880541413137961 0.0 0.0 0.0 0.0 34 0.03880541413137961 0.0 0.0 0.0 0.0 35 0.0403576306966348 0.0 0.0 0.0 0.0 36 0.0403576306966348 0.0 0.0 0.0 0.0 37 0.0403576306966348 0.0 0.0 0.0 0.0 38 0.0403576306966348 0.0 0.0 0.0 0.0 39 0.04190984726188998 0.0 0.0 0.0 0.0 40 0.04190984726188998 0.0 0.0 0.0 0.0 41 0.04190984726188998 0.0 0.0 0.0 0.0 42 0.045014280392400345 0.0 0.0 0.0 0.0 43 0.04656649695765553 0.0 0.0 0.0 0.0 44 0.0496709300881659 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTCA 25 1.9367666E-5 50.54495 41 ATACAAA 20 3.9682724E-4 50.46535 36 TATGCCT 40 2.4429028E-9 50.386005 35 TCGCCTA 20 4.077541E-4 50.188725 42 CTCAGGT 15 0.008270737 50.188725 42 CCATCTA 15 0.008296395 50.149452 33 CCCTCAC 15 0.008322111 50.11024 43 ATTTTAT 15 0.008322111 50.11024 44 CCTCAAC 20 4.1091954E-4 50.11024 43 CCGACGC 15 0.008322111 50.11024 43 CGACTCC 35 5.095535E-8 50.11024 44 CGTCACA 15 0.008322111 50.11024 44 CGATTCA 20 4.1250957E-4 50.0711 49 CGATTAA 40 2.582965E-9 50.0711 49 CAGAATG 15 0.008347888 50.071095 49 ACGATTA 40 2.5993359E-9 50.03201 48 AACGATT 55 0.0 50.03201 47 AGGGTGT 15 0.008373723 50.032005 50 ACCAAGC 60 0.0 50.032005 48 CGGTTGA 20 4.1730903E-4 49.954014 14 >>END_MODULE