FastQCFastQC Report
Fri 17 Jun 2016
SRR1526359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247398
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG34561.3969393446996337No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG30691.2405112409962895No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG26861.0856999652381991No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG22020.8900637838624402No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG19440.785778381393544No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG14500.5861001301546497No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG8350.33751283357181544No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG7050.28496592535105375No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG5280.21342128877355515No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG4790.19361514644419114No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG4500.18189314384109814No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG4030.16289541548436123No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC3970.16047017356647994No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCAGTCTCAAAGATTAAG3900.15764072466228504No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG3680.14874817096338694No Hit
CAAAGTGGGTACCCGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG3550.14349348014131078No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAACGATTAAG3450.13945141027817523No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCTCTTCTGCTTGGATC2630.10630643740046403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT150.00801704350.6288840
TTGCGAC150.00810872550.48348637
AAGGATT204.101999E-450.18495647
CACGATT3800.050.1849547
ACGATTA8850.050.1849548
TACGTAC150.008301116550.1849547
AACGATT8200.050.18494847
CAACGAT8200.050.17471746
CAATGAT451.3096724E-1050.17471746
TTAGGGG150.00840870650.02174833
GTCGCAT507.2759576E-1249.92028429
TCGTGAT150.00847647449.92028425
CGTAGCA150.00847647449.92028428
AGTAGCG204.210463E-449.92028428
CGTAAGA150.00848327349.9101632
GACTAAA150.0085173349.8596049
GGTACGT204.2740672E-449.768868
GGTGTTA204.2740672E-449.768868
GGCAATC150.00857888749.7688568
GGACTAA301.0784242E-649.7688568
Produced by FastQC (version 0.11.5)