##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526359_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 247398 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.85687434821624 38.0 37.0 38.0 33.0 40.0 2 35.53065909991188 38.0 36.0 38.0 29.0 40.0 3 35.29664346518565 38.0 36.0 38.0 27.0 40.0 4 34.56055424861963 38.0 35.0 38.0 19.0 40.0 5 35.545141836231494 38.0 35.0 38.0 28.0 40.0 6 33.85217342096541 36.0 33.0 38.0 24.0 39.0 7 36.80225790022555 38.0 38.0 39.0 33.0 40.0 8 36.47250584079095 38.0 36.0 39.0 31.0 40.0 9 36.55740143412639 38.0 37.0 39.0 31.0 40.0 10 35.459660142765905 38.0 35.0 39.0 28.0 40.0 11 33.45050081245604 36.0 31.0 38.0 24.0 40.0 12 32.01504054196073 35.0 29.0 38.0 17.0 40.0 13 31.787451798316884 35.0 29.0 38.0 16.0 40.0 14 30.69549875100041 35.0 26.0 38.0 12.0 40.0 15 31.463128238708478 35.0 28.0 38.0 15.0 40.0 16 32.323102854509735 36.0 29.0 39.0 17.0 40.0 17 32.447190357238135 36.0 30.0 39.0 18.0 40.0 18 32.875851866223655 36.0 30.0 39.0 20.0 40.0 19 33.443184666003766 36.0 31.0 39.0 23.0 40.0 20 33.71575356308458 36.0 31.0 39.0 24.0 40.0 21 35.369893855245394 38.0 35.0 39.0 27.0 40.0 22 35.10502105918399 38.0 35.0 39.0 26.0 40.0 23 35.40634524127115 38.0 35.0 39.0 28.0 40.0 24 34.83887501111569 38.0 34.0 39.0 25.0 40.0 25 35.145538767492056 38.0 35.0 39.0 27.0 40.0 26 35.593299056580896 38.0 35.0 39.0 29.0 40.0 27 35.60748267973064 38.0 35.0 39.0 29.0 40.0 28 35.12487974842157 38.0 35.0 39.0 28.0 40.0 29 35.223680062086196 38.0 35.0 39.0 28.0 40.0 30 34.72310204609577 38.0 34.0 39.0 27.0 40.0 31 34.56160114471419 38.0 34.0 39.0 26.0 40.0 32 33.48449462000501 38.0 33.0 39.0 19.0 40.0 33 34.795835051213025 38.0 35.0 39.0 27.0 40.0 34 34.55542890403318 38.0 34.0 39.0 26.0 40.0 35 34.714068019951654 38.0 34.0 39.0 27.0 40.0 36 32.88230300972522 36.0 32.0 38.0 24.0 39.0 37 32.72383366074099 36.0 32.0 38.0 23.0 39.0 38 31.994737225038197 35.0 31.0 38.0 18.0 39.0 39 30.130878180098463 35.0 28.0 38.0 8.0 39.0 40 26.049620449639853 32.0 14.0 38.0 4.0 39.0 41 22.63653303583699 26.0 10.0 33.0 4.0 37.0 42 26.40292565016694 30.0 23.0 34.0 4.0 37.0 43 21.854901818123025 26.0 9.0 33.0 4.0 36.0 44 23.691998318498936 28.0 12.0 33.0 4.0 37.0 45 26.36707653255079 31.0 23.0 35.0 4.0 38.0 46 25.85873774242314 30.0 19.0 36.0 4.0 38.0 47 25.230527328434345 30.0 16.0 36.0 4.0 38.0 48 25.245571912464936 30.0 17.0 35.0 4.0 38.0 49 22.93159605170616 28.0 7.0 35.0 4.0 38.0 50 25.156824226549933 30.0 17.0 35.0 4.0 38.0 51 26.682838988189072 32.0 18.0 38.0 4.0 40.0 52 26.844687507578882 33.0 17.0 38.0 4.0 40.0 53 26.40335410957243 33.0 11.0 38.0 4.0 40.0 54 25.322419744702866 31.0 4.0 38.0 4.0 39.0 55 25.56912343672948 32.0 4.0 38.0 4.0 39.0 56 24.98294246517757 32.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4484639574178289 1 2 2.9258185594744432 1 3 3.171875229277319 1 4 4.026760263803247 1 5 2.584359690948716 1 6 3.1084188993839703 1 7 1.6839072134162762 1 8 1.8955538945048502 1 9 1.8479852606785556 1 10 2.2894547712354267 1 11 -6.3272200740693485 1 12 -11.002855051689401 1 13 -21.75599464490277 1 14 -18.99879829627111 1 15 -13.563460249795128 1 16 -21.042216159121267 1 17 -21.526374773416762 1 18 -20.548757186052786 1 19 -6.289711273812593 1 20 -10.95358330131538 1 21 -7.524678702071842 1 22 -2.9958698746311008 1 23 -3.1486234855259205 1 24 -3.442471107645755 1 25 -3.4725315070568925 1 26 1.8644007536963656 1 27 1.8106904309334624 1 28 2.269464138141217 1 29 1.836124086374113 1 30 2.5411696495638694 1 31 1.4912455422264443 1 32 -11.174649223397196 1 33 1.012314859677545 1 34 0.94790153938213 1 35 1.08670671428591 1 36 3.4767704284507026 1 37 3.5174012955908935 1 38 4.665226032508464 1 39 6.224957883303627 1 40 8.54163930418774 1 41 0.4321018171674993 1 42 -5.576813098817123 1 43 -12.885321922281578 1 44 -16.548444775833698 1 45 -15.062867032236477 1 46 -18.635743632708795 1 47 -18.600837816078247 1 48 -17.13552878953667 1 49 -15.209080681525016 1 50 -17.079941039511645 1 51 -18.39164547251698 1 52 -18.60201242577691 1 53 -18.2902294504269 1 54 -18.755984374847742 1 55 -17.930689516155546 1 56 -17.1160943729498 2 1 1.721151831215174 2 2 3.277534776861984 2 3 3.6034865667134 2 4 4.459368394166425 2 5 3.1380131537206637 2 6 3.6432849824211573 2 7 2.032243002616241 2 8 2.366603560795909 2 9 2.2525315032026114 2 10 2.9946424283386577 2 11 -4.10252994665321 2 12 -11.488986628355779 2 13 -13.513102211861248 2 14 -15.576851516345656 2 15 -9.512320438752397 2 16 -3.251542673813127 2 17 -2.369769936804122 2 18 -9.715785009577097 2 19 -1.48694625691045 2 20 -2.80022887642156 2 21 2.795985248695253 2 22 3.329496338422551 2 23 2.9533961036220404 2 24 2.8591020916261556 2 25 2.7636867472553206 2 26 2.730028733240438 2 27 2.656815940164627 2 28 2.643954906971935 2 29 3.1129941565829995 2 30 3.1616949160976304 2 31 -1.3116494911444505 2 32 -10.976937513247247 2 33 -1.067645268605368 2 34 -0.739127150918641 2 35 -1.0529743119774153 2 36 4.526913446566329 2 37 4.718125053933619 2 38 5.679636191128559 2 39 7.577215835977629 2 40 10.373593018324861 2 41 7.902328045823129 2 42 4.10022599280115 2 43 -0.3491340051454088 2 44 -9.883583893455263 2 45 1.3628285558713493 2 46 -11.662266992334349 2 47 -13.104933334843958 2 48 -8.346892228905656 2 49 -11.609509735871901 2 50 -8.870202806002068 2 51 -7.961420977414267 2 52 -8.22986195338374 2 53 -8.797878752671853 2 54 -15.395925434048575 2 55 -9.030455486511793 2 56 -8.51123609090074 3 1 1.8454255759402685 3 2 3.30066791856585 3 3 3.5757206808348556 3 4 4.56767981242831 3 5 3.0350225109465185 3 6 3.5913462630767725 3 7 2.1637487209209283 3 8 2.4738141289568816 3 9 2.325325216125364 3 10 3.0626352116546656 3 11 0.6352383855588926 3 12 0.5636095431322019 3 13 -6.085743028936658 3 14 -6.007069851615668 3 15 -3.5531436299945227 3 16 3.032629548069327 3 17 3.0955074162629685 3 18 2.2846430987954776 3 19 1.363705079399253 3 20 1.5369217565672457 3 21 3.0428814629940746 3 22 3.5826459928779357 3 23 3.00464679165335 3 24 3.290100195717649 3 25 3.2145918906716275 3 26 2.396975708960575 3 27 2.468708179171415 3 28 2.1248123468838642 3 29 2.5372342642216807 3 30 2.3050194834023827 3 31 3.1833355250625104 3 32 2.0260592453607913 3 33 2.732214067215068 3 34 3.022217720954636 3 35 2.5819409837231504 3 36 4.916002798899516 3 37 4.659734129831115 3 38 5.798849083306408 3 39 7.87001138365056 3 40 10.717715701952777 3 41 10.245946173154792 3 42 7.8132099081580115 3 43 3.8962944090188643 3 44 3.4789627397781793 3 45 7.254410110516325 3 46 1.4160768398471681 3 47 -3.8899770630562998 3 48 1.5079178744519375 3 49 -10.158757488302944 3 50 0.7350354057281017 3 51 -0.8523145007461714 3 52 -0.6646060057352763 3 53 -1.3774916203863654 3 54 -3.15349377849234 3 55 -1.3612303626661557 3 56 2.238121746540397 4 1 2.1878455290835745 4 2 3.7187419130992367 4 3 4.052426557445557 4 4 4.815861674981996 4 5 3.3815546273073167 4 6 3.729475899937377 4 7 2.5508655698080815 4 8 2.7344789083399306 4 9 2.572909167706783 4 10 3.3332082300068677 4 11 3.9173669486201916 4 12 4.266081721968593 4 13 5.033729345135971 4 14 4.944841023042667 4 15 6.873105881914885 4 16 6.873804870209117 4 17 6.59350624484555 4 18 5.734516195242179 4 19 4.857701565147011 4 20 4.811911994755455 4 21 3.3798592060631876 4 22 3.72141014750504 4 23 3.264042535503407 4 24 3.9137823511256897 4 25 3.2597768770050877 4 26 2.9196719213775992 4 27 2.7492626500812207 4 28 2.353494990382501 4 29 2.6764499026441726 4 30 2.7365037668853915 4 31 3.291484197456107 4 32 3.992478612795388 4 33 3.031926093767197 4 34 3.18664323833066 4 35 2.878211971618505 4 36 4.687478784842508 4 37 5.006644516398865 4 38 5.3121373496086335 4 39 7.350182703447512 4 40 10.05547438996527 4 41 4.929870909585873 4 42 8.009690775006899 4 43 7.793185271963026 4 44 10.723691361410356 4 45 8.194240743081728 4 46 8.766891951131822 4 47 8.46292463549895 4 48 7.825750264143462 4 49 3.682832710838017 4 50 8.296010122635561 4 51 6.779079485977764 4 52 7.15032224494805 4 53 5.272962303861977 4 54 2.6303431016326115 4 55 7.018345486611469 4 56 6.488792871101115 5 1 2.0582435134521475 5 2 3.4547301895286893 5 3 3.74220185567674 5 4 4.6242095770799025 5 5 3.170517502667252 5 6 3.6123430328045103 5 7 2.320674804690846 5 8 2.5337641818899144 5 9 2.482848493087282 5 10 3.0773207351487883 5 11 5.018161374782267 5 12 6.7344203686160675 5 13 6.900643680800304 5 14 7.334945403955874 5 15 7.621820402688233 5 16 7.336885906819564 5 17 6.699378313952913 5 18 6.213794270108487 5 19 5.322376595998513 5 20 5.490747864767798 5 21 3.6848777169640528 5 22 3.7921115049711034 5 23 3.532579145951786 5 24 3.9469991343424766 5 25 3.2775550547097367 5 26 2.975873073166987 5 27 2.942187372417706 5 28 2.465036022879417 5 29 2.9718144645528497 5 30 3.2393960951453735 5 31 3.581269779521101 5 32 4.3643420434823454 5 33 3.2283730291847235 5 34 3.363975097647817 5 35 2.8953859740866292 5 36 4.239206468923133 5 37 4.4039861453581395 5 38 5.338888172315336 5 39 6.9870476767095475 5 40 6.232767684905625 5 41 2.3574855743328875 5 42 8.103350791461029 5 43 12.5615676249042 5 44 11.029018387325618 5 45 8.854629986191561 5 46 10.188709104566623 5 47 10.553126712833382 5 48 10.376128195036102 5 49 10.15869141096142 5 50 10.211541282652014 5 51 9.196397462119268 5 52 8.726229793282066 5 53 9.085102369678513 5 54 9.101304125902459 5 55 9.41513435950328 5 56 7.937977877271415 6 1 2.473573281723091 6 2 3.9142412666868296 6 3 4.226668369555014 6 4 5.09950878767323 6 5 3.5352997792142347 6 6 4.534420954882428 6 7 2.7323121869164666 6 8 2.946165506055941 6 9 2.940704362707855 6 10 3.9045425329588213 6 11 5.7509090192946175 6 12 7.4541810001414 6 13 7.234231661447108 6 14 8.821194449028443 6 15 8.100939322983624 6 16 7.023287740280221 6 17 7.128331713494553 6 18 6.323801909527894 6 19 5.264698979497368 6 20 5.01213823909935 6 21 3.477467480142046 6 22 3.8551367150551314 6 23 3.452619889521941 6 24 3.994745505618255 6 25 3.6238754010300838 6 26 2.8085697882166443 6 27 2.9668881618243788 6 28 3.2299965525458276 6 29 3.298399644079204 6 30 3.1561264236404014 6 31 3.7544299526812708 6 32 4.867397811243997 6 33 3.7097837753013536 6 34 3.7462007151675536 6 35 3.0964300280718575 6 36 3.2772762422870265 6 37 2.9656107952180832 6 38 4.24759711043604 6 39 5.468967717453115 6 40 1.436103564712095 6 41 0.7425123123008035 6 42 7.079031973024566 6 43 11.910307120702523 6 44 9.733118209072494 6 45 7.096544933988863 6 46 10.552727439173193 6 47 10.582665801196 6 48 10.47378543975217 6 49 11.204527927386174 6 50 10.584599596886797 6 51 9.42710792812385 6 52 9.505195925189376 6 53 9.543849504896233 6 54 10.398732188036405 6 55 10.10968490699501 6 56 9.068739272738693 7 1 2.215730144468189 7 2 3.4314681574431276 7 3 3.7889932995270073 7 4 4.589984978149417 7 5 3.207148518710035 7 6 3.825177257403439 7 7 2.5204074250117046 7 8 2.69686578616799 7 9 2.4766987604669595 7 10 3.50860415760868 7 11 5.465754957669965 7 12 6.954741224231039 7 13 7.3100019695703615 7 14 8.37371545743181 7 15 7.872087782367377 7 16 7.2459768159104705 7 17 6.94723927651976 7 18 6.094810312889237 7 19 5.3159594836990465 7 20 4.935667650864055 7 21 3.6199044549319623 7 22 3.712699740265215 7 23 3.2852529427432273 7 24 3.8825606316686745 7 25 3.322474840805995 7 26 2.8421832335947954 7 27 2.8617060889952484 7 28 2.871453135178882 7 29 3.0635256944993756 7 30 3.3704121379261167 7 31 3.5772590843339316 7 32 4.811935626370051 7 33 3.4805120666178766 7 34 3.854884188556909 7 35 3.3058137815732564 7 36 2.0426824047520107 7 37 1.6922214394757873 7 38 2.970706354133519 7 39 4.516726821094569 7 40 -2.237285791030203 7 41 -9.66925239358155 7 42 5.352281272744452 7 43 10.035097036668908 7 44 8.36645154240583 7 45 5.853827847154129 7 46 9.444324077828657 7 47 9.350452916041938 7 48 9.260620173645727 7 49 10.875536330747519 7 50 9.162610801368594 7 51 9.9544188524936 7 52 9.688529258522713 7 53 9.948471353635728 7 54 10.984726585795507 7 55 10.356043450412376 7 56 8.94492974892917 8 1 1.9956736472930459 8 2 3.459533960035351 8 3 3.7128051971674196 8 4 4.520460219093252 8 5 3.0614356572942825 8 6 3.7374072341398943 8 7 2.4896829318245963 8 8 2.563765088261704 8 9 2.5028037787454593 8 10 3.42982383191908 8 11 5.571471142448964 8 12 7.387808688504887 8 13 7.258373521580996 8 14 8.452894586710638 8 15 7.940848167877515 8 16 7.217894396502029 8 17 6.507076431720826 8 18 6.307256308236532 8 19 5.056039244416894 8 20 5.094723815702871 8 21 3.4361390843769613 8 22 3.8868273574137717 8 23 3.1653626137302666 8 24 3.791118292020954 8 25 3.2572372203340763 8 26 2.704928331634015 8 27 2.6753817287426713 8 28 1.9284488148066643 8 29 2.2097370604016646 8 30 2.4318943579327623 8 31 2.719083610754673 8 32 4.062882784894477 8 33 2.642177071602923 8 34 2.8827672061707332 8 35 2.4504631667510566 8 36 0.3672919095708913 8 37 0.20408584625544535 8 38 1.3891011696868567 8 39 2.7924496526000553 8 40 -2.312626435762674 8 41 -10.475998820966064 8 42 4.338705333894335 8 43 8.83591126066359 8 44 6.824843034595926 8 45 4.27973346363801 8 46 7.294407161909746 8 47 7.518050124416813 8 48 7.85374081173147 8 49 9.831219281894079 8 50 7.657143868832865 8 51 8.931404562031656 8 52 8.875917095013236 8 53 9.150814324722472 8 54 9.526135691810794 8 55 8.72272673389526 8 56 8.231038090570909 9 1 2.238907987033784 9 2 3.4501699238639816 9 3 3.9091699416124115 9 4 4.950077586621376 9 5 3.3958725797630294 9 6 4.097103170625871 9 7 2.6997848902835244 9 8 2.764264174094585 9 9 2.7402813363544567 9 10 3.255654406279575 9 11 5.09367469699427 9 12 7.062595794630628 9 13 6.992877977116233 9 14 8.25919822196619 9 15 7.928527501112018 9 16 6.804114356641048 9 17 6.280964195058601 9 18 5.799020568420019 9 19 5.0585593808007445 9 20 4.891918722721371 9 21 3.492216236787563 9 22 3.7774484969107363 9 23 3.0992213872638956 9 24 4.020813075503355 9 25 3.213711335403829 9 26 2.645647476434789 9 27 2.5308280234833234 9 28 2.7222505970207607 9 29 2.731429999078351 9 30 2.5708065814177985 9 31 2.9563964535673435 9 32 3.954689870925016 9 33 2.6776566387324365 9 34 2.6080311616318497 9 35 2.087283512322699 9 36 1.63113207033253 9 37 1.2793934511375937 9 38 2.2410802179647646 9 39 3.3952810999489706 9 40 -0.758945883638674 9 41 -4.16607969593106 9 42 5.320957314589762 9 43 9.927113843391599 9 44 8.37411746591809 9 45 5.381173070957935 9 46 9.370768941488404 9 47 9.806926934220641 9 48 9.657516074863352 9 49 11.424044305365939 9 50 9.444200580137242 9 51 9.411844679967448 9 52 9.088572132815287 9 53 9.438035750823179 9 54 9.783971998246201 9 55 8.855297437721585 9 56 7.9417742009957095 10 1 2.259759995214459 10 2 3.656270615739878 10 3 3.746104800843952 10 4 4.927737392548011 10 5 3.2005198971121374 10 6 3.7860201020039668 10 7 2.468036924572722 10 8 2.554986945079314 10 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114 52 -1.4832354473596432 114 53 -1.3179992345995615 114 54 -2.668214590675081 114 55 -0.8642506672346855 114 56 3.0108121018703464 115 1 1.043395119087478 115 2 2.442799898261285 115 3 1.4665836249972912 115 4 2.6590503708039392 115 5 2.151773779982058 115 6 2.2182498163762645 115 7 1.0901446600072262 115 8 1.402748139109164 115 9 1.0918226780542994 115 10 1.7707570390658276 115 11 -2.6541017159639537 115 12 -4.487151878187593 115 13 -2.8344739934036625 115 14 -7.606054623189273 115 15 -4.162280685832087 115 16 -11.190720705737782 115 17 -11.044086492366475 115 18 -2.23312339866645 115 19 -3.209898981514449 115 20 -3.4757685987626985 115 21 0.3779068437047357 115 22 0.6790926933855559 115 23 0.28637267997822136 115 24 1.1073590789663754 115 25 0.8262670175751765 115 26 1.7421533739351958 115 27 1.8367284837399467 115 28 1.716182905259501 115 29 1.7707368022927454 115 30 1.7491819199506011 115 31 0.3584624286158089 115 32 -5.687138724182358 115 33 0.6129247271135512 115 34 0.5653649290764662 115 35 0.4524993593576738 115 36 3.215495184686322 115 37 3.004911794805736 115 38 4.545041976707424 115 39 5.930925089234311 115 40 8.532436514135519 115 41 8.357621300058344 115 42 4.49341295384329 115 43 1.8176142059731788 115 44 1.4865769529761472 115 45 3.9980649328513493 115 46 -3.545852094162985 115 47 -3.968657266101985 115 48 -4.9514729884922275 115 49 -8.085153427389239 115 50 -1.9147107871652977 115 51 1.5816956515380909 115 52 1.4876782582241148 115 53 0.9879906131161746 115 54 1.3363539372437003 115 55 1.958566591648566 115 56 3.7482740308043567 116 1 1.1296190333570735 116 2 2.2919256737829983 116 3 2.214374563143565 116 4 2.7559435838139095 116 5 2.1781180176207116 116 6 2.3227807432422196 116 7 1.2283050284975445 116 8 1.6758962872573093 116 9 1.4787140110116255 116 10 1.6806085149071563 116 11 -2.246717809214566 116 12 -3.774071411934539 116 13 -5.406174501017876 116 14 -10.404606982655338 116 15 -7.128674744865737 116 16 -8.819927323523078 116 17 -10.878359852020544 116 18 -3.4182615825355995 116 19 -3.775489318043874 116 20 -4.066293133449673 116 21 1.4051441063480894 116 22 1.3008261019209897 116 23 1.2332376921985642 116 24 1.5412752286185665 116 25 1.2158844268626368 116 26 1.505854257559939 116 27 1.3507583765769482 116 28 1.5579999761374879 116 29 1.408732666176931 116 30 1.5969916586232884 116 31 -0.25525725553534784 116 32 -7.972977227660454 116 33 -0.4144770401359352 116 34 0.07116959160701697 116 35 -0.545221076814542 116 36 2.4239460213097495 116 37 2.7051844024387464 116 38 3.193636637357919 116 39 4.589766036780848 116 40 7.96679917629006 116 41 5.272196408161371 116 42 1.7014642792804011 116 43 -9.32949991322216 116 44 0.8123665750855658 116 45 1.225668805759792 116 46 -7.192331695591825 116 47 -9.08898063515632 116 48 -9.467429935286736 116 49 -5.541348200842895 116 50 -6.0450689371934985 116 51 -3.0567467248484483 116 52 -3.388050262174456 116 53 -4.38355479015512 116 54 -3.713584961045452 116 55 -3.2338802968643137 116 56 -0.2538175277592849 117 1 0.8424743305146976 117 2 2.228618180242947 117 3 2.15709635470256 117 4 3.008635228947412 117 5 2.1024289564665324 117 6 1.6983190722689159 117 7 1.0059751404699782 117 8 1.1701899995742835 117 9 1.1401050532166224 117 10 1.2546825889812254 117 11 -11.74984011756943 117 12 -12.12473893992765 117 13 -10.064120237451984 117 14 -10.54887916181124 117 15 -10.240862514030251 117 16 -14.056576763660892 117 17 -10.183700075965419 117 18 -3.5300186884787514 117 19 -3.7956228236424963 117 20 -4.0042776295737035 117 21 -0.12640197461659852 117 22 0.02261766092185269 117 23 -0.06962621822348325 117 24 -3.194297553465841 117 25 0.08097917277133604 117 26 1.3793469362851738 117 27 1.2096085057759112 117 28 0.9599164274725425 117 29 1.225700797098554 117 30 1.3907039755914141 117 31 -0.3072598395146784 117 32 -12.77347249036502 117 33 -0.0025476689042349676 117 34 -0.17484677359539802 117 35 -0.268627622895508 117 36 1.3763577353493694 117 37 1.0891206642819853 117 38 1.2400405994595545 117 39 3.9027505329048786 117 40 3.1273288920523328 117 41 4.906670999031309 117 42 1.6778854679108903 117 43 -6.217958138889687 117 44 0.7467980996333701 117 45 1.0616937842266303 117 46 -5.205789921259353 117 47 -7.2035370520383175 117 48 -5.515987213495176 117 49 -4.008617796019468 117 50 -3.9368648028265767 117 51 -2.8550886714462074 117 52 -2.472460253561195 117 53 -3.023735668708092 117 54 -7.3945711798223694 117 55 -4.268010357157163 117 56 -1.6174049523932226 118 1 1.1399236928552838 118 2 2.6826066773672252 118 3 2.507684001614294 118 4 3.4379925682822474 118 5 2.4405134775968165 118 6 2.53365290572728 118 7 1.2496610619503485 118 8 1.5156513649156125 118 9 1.3817904745719574 118 10 1.9966180728521934 118 11 -7.430260222595688 118 12 -8.574101280512796 118 13 -7.356443318222176 118 14 -1.2731852383303668 118 15 -17.529809750839455 118 16 -7.160727801420332 118 17 -12.574297725377772 118 18 -2.842096707983629 118 19 -3.393022173479956 118 20 -4.066293133449673 118 21 0.639164416981302 118 22 0.862952744692798 118 23 0.655430045842536 118 24 -2.6976733974268328 118 25 0.8818043790729106 118 26 1.4629928477630472 118 27 1.8700236095518576 118 28 1.7373607885628104 118 29 1.8466060234051298 118 30 1.7660478115504077 118 31 -0.33814256019484645 118 32 -6.979398971985425 118 33 0.5422135213933359 118 34 0.8679139165771446 118 35 0.2183691262917904 118 36 3.6661801909632707 118 37 3.621253697540304 118 38 4.351193387656608 118 39 6.631432703447512 118 40 8.920652222885039 118 41 7.822524962522181 118 42 2.722521626950652 118 43 0.07611537948990588 118 44 1.6945350338669307 118 45 1.9749220913034016 118 46 -6.723544359869003 118 47 -7.620641328107334 118 48 -8.903959206493427 118 49 -6.3579551303530515 118 50 -6.13153847124368 118 51 -5.337959389125629 118 52 -5.078611791445663 118 53 -5.459719664707091 118 54 -8.38756942938476 118 55 -6.895917333901352 118 56 -3.2384352099576112 119 1 1.3405904796637174 119 2 2.681435295136197 119 3 2.868109303470483 119 4 3.643254777689343 119 5 2.4345063892325456 119 6 1.9973100211234538 119 7 0.9733650326357619 119 8 1.2798431111762056 119 9 1.0505896576079436 119 10 1.4483511178452844 119 11 -8.449723188895923 119 12 -6.900105966041718 119 13 -1.9571521546491617 119 14 -1.7845987062224822 119 15 -7.747596739486056 119 16 -9.506405701342239 119 17 -11.561682839812807 119 18 -2.005008944422624 119 19 -2.4151282586604665 119 20 -2.673369483542782 119 21 -1.0219456929484139 119 22 -0.7838004240463192 119 23 -0.6417105800960741 119 24 -5.459552414667176 119 25 -1.3642159248857553 119 26 1.0755056128258218 119 27 1.1355969861362283 119 28 0.9634675945939293 119 29 1.2745509984757106 119 30 1.276590251629699 119 31 -0.9331746981175968 119 32 -8.46239891191454 119 33 0.15292415994682784 119 34 -0.7373603070066821 119 35 -0.04819541987268394 119 36 2.7969545046534243 119 37 2.654232612660145 119 38 3.66184996241482 119 39 5.916348904564831 119 40 7.758310659840646 119 41 7.469097918610366 119 42 2.8174636585479362 119 43 0.3612718641417949 119 44 1.5110785552221273 119 45 1.508231396283275 119 46 -6.989688409847979 119 47 -8.483559571634697 119 48 -10.4354363495222 119 49 -5.97968877605498 119 50 -6.249367509508833 119 51 -7.29590977057574 119 52 -7.539715894287017 119 53 -10.050066273705708 119 54 -8.437488333691842 119 55 -10.681764633715131 119 56 -6.531310803157588 120 1 1.1171921327722814 120 2 2.69251046910464 120 3 2.885914524157208 120 4 3.7079416344961658 120 5 2.451266165768857 120 6 2.1025078387587897 120 7 0.970751422259724 120 8 0.981695507530219 120 9 0.8410434457094809 120 10 0.8804135831332758 120 11 -10.291795781924115 120 12 -3.403944706281518 120 13 -6.608659881134834 120 14 -8.982579692206997 120 15 -9.698362854027533 120 16 -11.028016992139065 120 17 -10.686352054749591 120 18 -3.560074448032676 120 19 -3.2596511114161935 120 20 -5.060445180233298 120 21 -0.7861326967708209 120 22 -0.68552867390747 120 23 -0.7913724442536747 120 24 -7.059114634929191 120 25 -1.025099978595449 120 26 0.3949868111661772 120 27 0.6687408327172975 120 28 0.8886529975799817 120 29 0.9243272080872629 120 30 0.6622938028727958 120 31 -2.3963391268803775 120 32 -8.913279371909965 120 33 -2.3944965333133226 120 34 -8.672738790459263 120 35 -1.6560885232083038 120 36 0.1641994326157885 120 37 0.1822799190079465 120 38 0.07643390831308494 120 39 1.976461943213593 120 40 0.330103269857311 120 41 2.082381593346554 120 42 -3.3986819195500075 120 43 -3.796361511662706 120 44 -5.043627576961217 120 45 -9.38519864063397 120 46 -12.883608498710732 120 47 -12.962274982134877 120 48 -11.28492506199239 120 49 -15.78598757706122 120 50 -10.180302855322156 120 51 -10.559427036739287 120 52 -10.28083778654093 120 53 -11.288525550389037 120 54 -10.675816929856369 120 55 -11.135683415772695 120 56 -6.538661582340183 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3297.0 5 100.0 6 101.0 7 219.0 8 445.0 9 522.0 10 272.0 11 278.0 12 306.0 13 390.0 14 542.0 15 843.0 16 1290.0 17 1669.0 18 2151.0 19 2453.0 20 3126.0 21 4045.0 22 4943.0 23 5889.0 24 6568.0 25 6901.0 26 7251.0 27 7577.0 28 7791.0 29 8577.0 30 10000.0 31 12110.0 32 14651.0 33 17370.0 34 20411.0 35 24406.0 36 27879.0 37 25693.0 38 13901.0 39 3431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.734436009992 0.18027631589584395 0.06386470383754113 0.021422970274618227 8 99.57800790628866 0.3338749706949935 0.05295111520707523 0.035166007809278976 9 98.36582545625086 0.9658691204890395 0.28664763768385476 0.38165778557624663 10 38.14460909142354 30.874542235588 21.710765648873476 9.270083024114989 11 27.204782654389355 37.271720413591275 20.47624437132671 15.047252560692662 12 25.49333462679569 12.377626334893572 20.300487473625495 41.82855156468525 13 20.8882044317254 10.934607393754193 23.270196202071155 44.90699197244925 14 18.546968808748915 13.980876912814086 37.1561988315915 30.3159554468455 15 34.750888671163324 16.015919157240138 22.24066591632321 26.992526255273326 16 39.59650938970933 17.171198421299515 20.377529398443944 22.854762790547205 17 23.193146165539993 19.262865090403338 35.8799366044571 21.664052139599573 18 22.279174869330056 20.768133107499022 37.056096111634375 19.89659591153655 19 37.84369453686896 23.108239774569537 18.830074105818095 20.217991582743412 20 22.97393470331821 37.35271045118924 18.788768388448133 20.884586457044424 21 22.20546798885866 20.948226723855647 35.6332898082606 21.213015479025092 22 20.531942844439055 19.57921542472564 22.647587865559128 37.24125386527618 23 20.313422097187527 21.78756497627305 21.450052142701235 36.44896078383819 24 20.633554030347863 21.539786093662844 36.07021883766239 21.75644103832691 25 36.87029349587909 22.849509088630825 19.39069592598132 20.889501489508767 26 21.154726180785882 22.163096872430984 21.287960701890796 35.39421624489234 27 20.07812089329543 19.180862807257316 21.452110517611573 39.28890578183568 28 20.307684831599452 35.29588039248242 22.875099720176074 21.52133505574206 29 36.795691359672 21.137649251769997 22.020322096742238 20.046337291815767 30 22.008305936682397 20.36531563772529 38.12643301670488 19.499945408887434 31 21.629057550254664 34.060123261194605 25.328514179899752 18.982305008650975 32 36.380101473590585 19.058033999717004 22.531584160417214 22.030280366275193 33 22.118621069558138 20.66329940418086 24.121561686115513 33.09651784014549 34 19.24211463370223 35.42355392801505 26.82946567665446 18.50486576162826 35 19.62230113981087 23.424367198686266 40.21938730109936 16.733944360403505 36 19.953923208046028 36.959012709582595 24.164807534243803 18.92225654812757 37 20.083241135872715 22.290711764395976 39.22464975096554 18.40139734876577 38 34.91706175528663 24.131243648553998 23.269268920729903 17.68242567542947 39 20.39072484937009 18.901669608260807 20.519749254458038 40.18785628791106 40 13.252281860631598 15.121699233685307 25.999788490636643 45.62623041504645 41 16.026187514021743 17.10958373274051 29.152169035916053 37.712059717321694 42 30.561857842025088 22.73239231193077 26.195993906589308 20.509755939454834 43 11.666612581330824 12.877042397494767 29.266116077947785 46.19022894322662 44 13.53930917911691 14.405984243010325 18.58414630677778 53.47056027109498 45 19.25136286924954 22.61047638705082 38.2777056040536 19.86045513964604 46 17.61805170691057 24.367619872087896 25.057746530151576 32.956581890849954 47 15.827352673607601 31.50065703022339 25.599110482159105 27.072879814009905 48 19.952767814176937 35.510677789954265 25.369911318336037 19.166643077532765 49 13.06509432431994 21.888432902399067 17.893234277928272 47.153238495352724 50 30.96240412626768 22.18800773067433 27.57435911980519 19.2752290232528 51 19.912121974374994 34.817711925607966 27.22717646199661 18.042989638020433 52 19.340082154860287 22.664431491614035 37.792453442873146 20.20303291065253 53 19.71028230461677 22.187789889857047 38.10160373651569 20.000324069010496 54 18.000412853505814 29.604429675262384 23.45898381371402 28.936173657517777 55 18.40840342259675 35.58739324632411 26.534438563740398 19.46976476733874 56 30.697781810522223 22.297746163941863 25.60437156699127 21.400100458544646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 0.0 8 6.5 9 13.0 10 48.0 11 83.0 12 183.5 13 402.5 14 521.0 15 594.5 16 668.0 17 751.0 18 834.0 19 896.5 20 959.0 21 1147.5 22 1484.0 23 1632.0 24 1954.5 25 2277.0 26 2763.0 27 3249.0 28 3510.5 29 4097.5 30 4423.0 31 4419.0 32 4415.0 33 4341.5 34 4268.0 35 4290.0 36 4312.0 37 4362.5 38 4610.0 39 4807.0 40 5110.5 41 5414.0 42 6163.0 43 6912.0 44 10056.5 45 13201.0 46 17723.0 47 19953.5 48 17662.0 49 15796.0 50 13930.0 51 13720.0 52 13510.0 53 13529.0 54 13151.0 55 12754.0 56 12854.5 57 12955.0 58 13553.5 59 14152.0 60 13897.5 61 13643.0 62 12622.0 63 10706.5 64 9812.0 65 8408.5 66 7005.0 67 6234.5 68 5464.0 69 4780.5 70 4097.0 71 3594.5 72 2478.0 73 1864.0 74 1434.0 75 1004.0 76 789.5 77 575.0 78 489.0 79 300.5 80 198.0 81 131.5 82 65.0 83 40.5 84 16.0 85 11.0 86 6.0 87 3.0 88 0.0 89 0.0 90 0.0 91 0.0 92 1.5 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02263559123355888 10 0.0 11 0.0016168279452542058 12 0.0 13 0.0 14 0.021422970274618227 15 0.1616827945254206 16 0.042845940549236454 17 0.02506083315144019 18 0.007679932739957477 19 0.01980614232936402 20 0.03880387068610094 21 0.012530416575720096 22 0.0012126209589406543 23 0.0 24 0.0 25 4.0420698631355145E-4 26 0.1879562486358014 27 0.03597442178190608 28 0.184722592745293 29 0.03274076589139766 30 0.0424417335629229 31 0.2445452267196986 32 0.017380900411482714 33 0.20654977000622476 34 0.10630643740046403 35 0.06628994575542244 36 0.16936272726537804 37 0.26071350617224065 38 0.16451224342961543 39 0.3771251182305435 40 0.6249040008407506 41 0.9066362703012958 42 0.2320148101439785 43 0.35327690603804396 44 0.5194059774129136 45 0.1248999587708874 46 0.07962877630376963 47 0.029507110000889254 48 0.04405856150817711 49 0.32417400302346827 50 0.2380779149386818 51 0.3718704274084673 52 0.4179500238482122 53 0.21705915165037712 54 0.13621775438766684 55 0.18229735082741172 56 0.21422970274618228 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 247398.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.75574352656433 #Duplication Level Percentage of deduplicated Percentage of total 1 92.64277485167919 68.32936741549693 2 4.644223207345344 6.850762715221624 3 1.1362872299565825 2.5142312851556374 4 0.4519641470662392 1.3333980685687976 5 0.2632859111848847 0.9709424069755076 6 0.18083611353516713 0.8002621206144274 7 0.10972200269396337 0.5664839522941874 8 0.07164499632717047 0.4227383979254743 9 0.060773654788682414 0.4034165486189416 >10 0.3753331573490585 5.036539104554917 >50 0.03488546884071715 1.7767034118866623 >100 0.022855996826676755 3.509044691141947 >500 0.0018044208021060595 0.9174089028168834 >1k 0.003608841604212119 6.568700978728042 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 3456 1.3969393446996337 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 3069 1.2405112409962895 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 2686 1.0856999652381991 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 2202 0.8900637838624402 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 1944 0.785778381393544 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 1450 0.5861001301546497 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG 835 0.33751283357181544 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 705 0.28496592535105375 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG 528 0.21342128877355515 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 479 0.19361514644419114 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 450 0.18189314384109814 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG 403 0.16289541548436123 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 397 0.16047017356647994 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCAGTCTCAAAGATTAAG 390 0.15764072466228504 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG 368 0.14874817096338694 No Hit CAAAGTGGGTACCCGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 355 0.14349348014131078 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAACGATTAAG 345 0.13945141027817523 No Hit CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCTCTTCTGCTTGGATC 263 0.10630643740046403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.004850483835762617 0.0 0.0 0.0 0.0 11 0.010509381644152337 0.0 0.0 0.0 0.0 12 0.01131779561677944 0.0 0.0 0.0 0.0 13 0.01131779561677944 0.0 0.0 0.0 0.0 14 0.012530416575720094 0.0 0.0 0.0 0.0 15 0.013338830548347197 0.0 0.0 0.0 0.0 16 0.0141472445209743 0.0 0.0 0.0 0.0 17 0.017380900411482714 0.0 0.0 0.0 0.0 18 0.020210349315677573 0.0 0.0 0.0 0.0 19 0.023039798219872433 0.0 0.0 0.0 0.0 20 0.024252419178813087 0.0 0.0 0.0 0.0 21 0.026273454110380845 0.0 0.0 0.0 0.0 22 0.02789028205563505 0.0 0.0 0.0 0.0 23 0.03233655890508411 0.0 0.0 0.0 0.0 24 0.035166007809278976 0.0 0.0 0.0 0.0 25 0.03678283575453318 0.0 0.0 0.0 0.0 26 0.03880387068610094 0.0 0.0 0.0 0.0 27 0.041229112603982244 0.0 0.0 0.0 0.0 28 0.048100631371312624 0.0 0.0 0.0 0.0 29 0.04890904534393972 0.0 0.0 0.0 0.0 30 0.050121666302880376 0.0 0.0 0.0 0.0 31 0.05093008027550748 0.0 0.0 0.0 0.0 32 0.051334287261821036 0.0 0.0 0.0 0.0 33 0.051334287261821036 0.0 0.0 0.0 0.0 34 0.051334287261821036 0.0 0.0 0.0 0.0 35 0.051738494248134585 0.0 0.0 0.0 0.0 36 0.051738494248134585 0.0 0.0 0.0 0.0 37 0.051738494248134585 0.0 0.0 0.0 0.0 38 0.051738494248134585 0.0 0.0 0.0 0.0 39 0.051738494248134585 0.0 0.0 0.0 0.0 40 0.051738494248134585 0.0 0.0 0.0 0.0 41 0.053759529179702344 0.0 0.0 0.0 0.0 42 0.054972150138643 0.0 0.0 0.0 0.0 43 0.056993185070210756 0.0 0.0 0.0 0.0 44 0.061035254933346265 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCAAT 15 0.008017043 50.62888 40 TTGCGAC 15 0.008108725 50.483486 37 AAGGATT 20 4.101999E-4 50.184956 47 CACGATT 380 0.0 50.18495 47 ACGATTA 885 0.0 50.18495 48 TACGTAC 15 0.0083011165 50.18495 47 AACGATT 820 0.0 50.184948 47 CAACGAT 820 0.0 50.174717 46 CAATGAT 45 1.3096724E-10 50.174717 46 TTAGGGG 15 0.008408706 50.021748 33 GTCGCAT 50 7.2759576E-12 49.920284 29 TCGTGAT 15 0.008476474 49.920284 25 CGTAGCA 15 0.008476474 49.920284 28 AGTAGCG 20 4.210463E-4 49.920284 28 CGTAAGA 15 0.008483273 49.91016 32 GACTAAA 15 0.00851733 49.859604 9 GGTACGT 20 4.2740672E-4 49.76886 8 GGTGTTA 20 4.2740672E-4 49.76886 8 GGCAATC 15 0.008578887 49.768856 8 GGACTAA 30 1.0784242E-6 49.768856 8 >>END_MODULE