SRR1526358_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45230
Sequences flagged as poor quality	0
Sequence length	56
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG	433	0.9573292062790183	No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG	399	0.8821578598275481	No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG	399	0.8821578598275481	No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG	393	0.868892328100818	No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG	391	0.8644704841919081	No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG	374	0.8268848109661728	No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG	351	0.7760336060137077	No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG	312	0.6898076497899623	No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG	280	0.6190581472474022	No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG	262	0.579261552067212	No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG	240	0.5306212690692018	No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG	238	0.5261994251602918	No Hit
CGAGTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG	174	0.38470042007517136	No Hit
CGAGTAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG	131	0.289630776033606	No Hit
CGAGTAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG	121	0.26752155648905596	No Hit
CGAGTAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG	111	0.24541233694450587	No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTAACCACAGTTCTGAGTCG	75	0.16581914658412558	No Hit
CGAGTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG	74	0.16360822462967056	No Hit
CGAGTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	72	0.15918638072076058	No Hit
CGAGTAGGGATGCGCTGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG	71	0.15697545876630553	No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACTCACAGTTCTGAGTCG	67	0.1481317709484855	No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAACCACAGTTCTGAGTCG	65	0.14370992703957552	No Hit
CGAGTAGGGATGCGCTGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG	52	0.11496794163166041	No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACTCACAGTTCTGAGTCG	52	0.11496794163166041	No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACTCACAGTTCTGAGTCG	49	0.10833517576829539	No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTAACCACAGTTCTGAGTCG	48	0.10612425381384036	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCAAG	35	4.6373316E-8	50.653156	40
TACACAC	35	4.6373316E-8	50.653156	38
ACACAGT	45	1.1459633E-10	50.653156	41
TAACCAC	35	4.6373316E-8	50.653156	38
TATCCAC	20	3.883418E-4	50.653156	38
AACTAAA	20	3.883418E-4	50.653156	38
CCACAGT	270	0.0	50.653156	41
GCCAAGT	35	4.6373316E-8	50.653156	41
TCACAGT	20	3.883418E-4	50.653156	41
TACTCAC	20	3.883418E-4	50.653156	38
ACGCCTA	15	0.00795911	50.653152	39
CCAACCC	15	0.00795911	50.653152	39
AACAACC	15	0.00795911	50.653152	38
CGCCTAG	15	0.00795911	50.653152	40
CAACCCA	15	0.00795911	50.653152	40
CCGCCCA	100	0.0	50.653152	39
ACCAACC	25	1.9030522E-5	50.653152	38
TACGCCT	15	0.00795911	50.653152	38
TCCGCCC	110	0.0	50.653152	38
TACTTTT	15	0.00795911	50.653152	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content