##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526358_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45230 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.92429803227946 38.0 36.0 39.0 33.0 40.0 2 35.823347335839046 38.0 35.0 39.0 29.0 40.0 3 34.86710148131771 38.0 34.0 38.0 27.0 40.0 4 35.66663718770727 38.0 35.0 39.0 28.0 40.0 5 33.924519124474905 36.0 32.0 39.0 23.0 40.0 6 34.0813398187044 38.0 35.0 39.0 17.0 40.0 7 35.9772717223082 38.0 35.0 38.0 30.0 40.0 8 35.81576387353527 38.0 35.0 39.0 30.0 40.0 9 35.867388901171786 38.0 35.0 39.0 30.0 40.0 10 35.0653106345346 38.0 34.0 38.0 27.0 40.0 11 34.45604687154543 38.0 33.0 39.0 25.0 40.0 12 32.31233694450586 36.0 29.0 39.0 17.0 40.0 13 31.792947158965287 36.0 28.0 39.0 15.0 40.0 14 30.885009949148795 36.0 26.0 39.0 12.0 40.0 15 31.926420517355737 36.0 28.0 39.0 17.0 40.0 16 32.22432014149901 36.0 29.0 39.0 17.0 40.0 17 32.788856953349544 36.0 30.0 39.0 18.0 40.0 18 32.944970152553616 36.0 30.0 39.0 20.0 40.0 19 33.66962193234579 36.0 31.0 39.0 24.0 40.0 20 33.781450364802126 37.0 31.0 39.0 24.0 40.0 21 35.502852089321244 38.0 35.0 40.0 27.0 40.0 22 35.193765200088436 38.0 35.0 39.0 27.0 40.0 23 35.168693345124915 38.0 35.0 39.0 27.0 40.0 24 34.71667035153659 38.0 34.0 39.0 24.0 40.0 25 35.1417864249392 38.0 35.0 39.0 27.0 40.0 26 35.29159849657307 38.0 35.0 39.0 27.0 40.0 27 35.46296705726288 38.0 35.0 39.0 28.0 40.0 28 34.93670130444395 38.0 35.0 39.0 27.0 40.0 29 35.164757904045985 38.0 35.0 39.0 27.0 40.0 30 34.65376962193235 38.0 34.0 39.0 25.0 40.0 31 34.82321468052178 38.0 35.0 39.0 26.0 40.0 32 33.431372982533716 38.0 33.0 39.0 19.0 40.0 33 34.7162281671457 38.0 34.0 39.0 27.0 40.0 34 34.64072518240106 38.0 34.0 39.0 26.0 40.0 35 34.814879504753485 38.0 35.0 39.0 27.0 40.0 36 33.04875082909573 36.0 32.0 38.0 24.0 39.0 37 32.774751271280124 36.0 31.0 38.0 23.0 39.0 38 31.118195887685165 35.0 30.0 38.0 16.0 39.0 39 30.40519566659297 35.0 29.0 38.0 9.0 39.0 40 26.814724740216672 33.0 15.0 38.0 4.0 39.0 41 22.23318593853637 26.0 10.0 33.0 4.0 37.0 42 25.869246075613532 30.0 21.0 34.0 4.0 37.0 43 22.955847888569533 28.0 10.0 33.0 4.0 37.0 44 23.879261552067213 29.0 11.0 34.0 4.0 36.0 45 25.956356400619057 30.0 22.0 34.0 4.0 37.0 46 26.519013928808313 31.0 21.0 36.0 4.0 38.0 47 25.48140614636303 30.0 16.0 35.0 4.0 38.0 48 25.95856732257351 31.0 19.0 36.0 4.0 38.0 49 23.372407694008402 29.0 7.0 36.0 4.0 38.0 50 25.975679858500996 31.0 19.0 36.0 4.0 38.0 51 27.001569754587663 33.0 18.0 38.0 4.0 40.0 52 26.811585231041345 33.0 17.0 38.0 4.0 40.0 53 26.59767853194782 33.0 15.0 38.0 4.0 40.0 54 25.587972584567765 32.0 5.0 38.0 4.0 40.0 55 25.924275923059916 32.0 4.0 38.0 4.0 40.0 56 26.28536369666151 34.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4960499994179273 1 2 2.546252117038442 1 3 3.094882610293368 1 4 2.8811885005425566 1 5 4.0698984713081785 1 6 4.863298749391788 1 7 2.3649754400342005 1 8 2.4690677670460843 1 9 2.4868189528940974 1 10 2.7129082217268348 1 11 -2.62772151506897 1 12 -5.57806316030857 1 13 -19.915324674057324 1 14 -15.493792961948213 1 15 -10.652768985232978 1 16 -21.0026273070633 1 17 -20.485191333752326 1 18 -19.893123721668005 1 19 -6.669795954983378 1 20 -9.556165291016484 1 21 -7.78200767974004 1 22 -2.462675912413161 1 23 -3.079480638184421 1 24 -2.369884655703377 1 25 -2.835379960891114 1 26 2.517084436064927 1 27 2.595584223414619 1 28 2.883718459235368 1 29 2.559748064740141 1 30 2.658392599469714 1 31 1.7468041573988984 1 32 -11.755373206219954 1 33 1.5638036704895626 1 34 1.7338010210122263 1 35 1.7665214732309025 1 36 3.643301515000747 1 37 3.3565078512481286 1 38 4.0878750161006145 1 39 5.6376076291183 1 40 9.155226336818984 1 41 -3.038154137871228 1 42 -9.19166577292942 1 43 -14.941898569516026 1 44 -16.42672248525801 1 45 -14.811896961990056 1 46 -19.20164825894877 1 47 -18.212930017875557 1 48 -17.101480913056314 1 49 -14.891329255070357 1 50 -16.816133032666887 1 51 -17.338295856101528 1 52 -17.6306682829293 1 53 -17.781790193021248 1 54 -18.667336667032878 1 55 -17.86776207743521 1 56 -18.020931056562397 2 1 1.6946513980193245 2 2 2.6791192499055754 2 3 3.2864910019017657 2 4 2.8252444445985034 2 5 3.7957725971823066 2 6 5.243718329811365 2 7 2.446094321153076 2 8 2.859277557255872 2 9 2.6868189528941 2 10 3.1059152147338267 2 11 -2.79555368290114 2 12 -10.355685537930949 2 13 -14.423016981749631 2 14 -15.015471283626535 2 15 -5.1688529013168925 2 16 -4.4389909434269335 2 17 -1.3243521729131658 2 18 -10.231585260129542 2 19 -2.1033623885498116 2 20 -3.8498715847227807 2 21 2.8851251873928305 2 22 3.3750863253490806 2 23 3.137302578598799 2 24 3.5979475121287905 2 25 3.0275571020459466 2 26 2.8695319885124775 2 27 2.6739059017362976 2 28 2.4053967809136907 2 29 3.078629183621267 2 30 3.146504487581602 2 31 1.5482027587975011 2 32 -8.453275304122052 2 33 1.1693980760839722 2 34 1.4540807412919463 2 35 1.2965914033008303 2 36 5.311832983532209 2 37 4.755109249849529 2 38 5.307455435681032 2 39 6.924320915831586 2 40 9.365016546609198 2 41 8.142265442548354 2 42 3.139802758539112 2 43 1.5783811507636933 2 44 -10.514834373369897 2 45 1.6384526883595925 2 46 -8.99325665055716 2 47 -9.7541887591343 2 48 -7.469313080888483 2 49 -10.418601982343084 2 50 -6.881867298401154 2 51 -6.843190960996633 2 52 -6.963535415796432 2 53 -6.118853130084183 2 54 -14.835168834865048 2 55 -7.209020818693951 2 56 -6.212539448170791 3 1 1.3069590903270196 3 2 2.2032218140081383 3 3 2.5203371557479173 3 4 2.5708854702395243 3 5 3.034746956156667 3 6 4.949359355452394 3 7 2.332248167306922 3 8 2.5587647367430506 3 9 2.433485619560763 3 10 3.1541203429389526 3 11 2.4547027273552686 3 12 3.2340580518126423 3 13 -5.901991340723988 3 14 -5.051368719523971 3 15 -4.467314439778431 3 16 2.6943423899063994 3 17 2.446929878368884 3 18 2.2002096116653327 3 19 0.2586888935014713 3 20 1.1880771332259386 3 21 2.614355956623598 3 22 2.9761119663747166 3 23 2.909610270906491 3 24 3.467691101872383 3 25 2.820377614866466 3 26 2.527993526974015 3 27 2.491341799172197 3 28 2.094627550144459 3 29 2.8991420041340845 3 30 1.74445320553032 3 31 2.4025617331564746 3 32 1.8082631574164054 3 33 2.520167306853203 3 34 2.7156192028304105 3 35 2.0053093520187844 3 36 3.788756060455288 3 37 4.9807502754905535 3 38 4.908481076706675 3 39 7.054577326088001 3 40 9.55886270045535 3 41 9.621239801522712 3 42 7.969546348282702 3 43 0.7732529456354911 3 44 3.9805502420147185 3 45 6.275375765282671 3 46 -0.560436137736648 3 47 -5.84444516939071 3 48 -1.4057233372987383 3 49 -10.498601982343084 3 50 -1.1434057599396148 3 51 -2.560114037919707 3 52 -2.1809713132323303 3 53 -2.192699283930338 3 54 -7.47824575794197 3 55 -2.8992772289503606 3 56 0.19361439798305824 4 1 2.2067121767467697 4 2 3.23334527079826 4 3 3.721818637229397 4 4 3.5397743591284154 4 5 4.673265474675183 4 6 5.980470466563503 4 7 3.361383969776057 4 8 3.3523449836566286 4 9 3.407806607215086 4 10 4.15510799725994 4 11 4.807295319947862 4 12 5.903193854281778 4 13 6.156280264214285 4 14 7.000483132327879 4 15 7.016142350345024 4 16 7.277058439289117 4 17 6.638040989479997 4 18 5.716752821541871 4 19 5.4033802515261655 4 20 5.475484540633346 4 21 3.7407757097100145 4 22 3.95932184291793 4 23 3.7209682955978494 4 24 5.153123200637815 4 25 4.135439343261524 4 26 3.3018206874678455 4 27 3.013317107814167 4 28 2.814133722983968 4 29 4.032475337467417 4 30 3.6175396252834062 4 31 4.17194444920586 4 32 5.057645873465788 4 33 4.1557228624087585 4 34 4.085989573200784 4 35 4.151482191524956 4 36 5.89492889996146 4 37 5.8449478063547495 4 38 5.785518113743716 4 39 8.141984733495406 4 40 10.726270107862753 4 41 5.11111634473259 4 42 9.845595730998749 4 43 7.795475167857713 4 44 10.93264900744682 4 45 9.191918975159215 4 46 10.1640083067078 4 47 10.277530139251262 4 48 9.817486539244467 4 49 6.4431264127186445 4 50 10.187458437591253 4 51 8.889762505290165 4 52 9.104460785533103 4 53 8.080387135822747 4 54 1.399778933416055 4 55 9.025167215494086 4 56 8.692873657242316 5 1 1.980988941073285 5 2 2.5875004209733135 5 3 3.1806356632106016 5 4 2.7503879578017134 5 5 4.010468349191491 5 6 4.9688618430145794 5 7 2.3250839882024437 5 8 2.6278194631112086 5 9 2.355475669312007 5 10 3.018199944929002 5 11 4.881369394021938 5 12 7.55843616126538 5 13 7.571242490121779 5 14 8.457088991918816 5 15 6.743431828878283 5 16 7.128173235677547 5 17 5.561755749015653 5 18 6.088965830570803 5 19 4.7269475999691295 5 20 5.286982605862754 5 21 3.4304753596086712 5 22 3.097704006175711 5 23 3.3131923604587286 5 24 3.691173688937056 5 25 3.5214721422296478 5 26 2.8676950195113307 5 27 2.197212445938362 5 28 2.825373818801175 5 29 3.122027576273389 5 30 3.292313902047738 5 31 3.5006711858927915 5 32 3.9037855454761115 5 33 2.8389732770024523 5 34 2.5812908446214564 5 35 2.8194387052526153 5 36 3.687263523141855 5 37 4.214581121261698 5 38 4.340520877701703 5 39 6.46094548529198 5 40 4.8711015064254966 5 41 1.786811940826194 5 42 8.821088636839914 5 43 10.83693453767529 5 44 11.127415913656506 5 45 8.584032481700579 5 46 8.903643464253399 5 47 10.162918014688891 5 48 9.801639846780862 5 49 10.88886070422408 5 50 9.671519613194711 5 51 8.082871036707154 5 52 7.945993860897023 5 53 8.043718626517421 5 54 7.295485585341613 5 55 8.540921776024764 5 56 7.463465144251714 6 1 2.1932090903270165 6 2 3.3598884806748046 6 3 3.162212155747916 6 4 3.458177136906194 6 5 4.31620528949 6 6 5.94227602211906 6 7 3.2984981673069242 6 8 3.4491814034097175 6 9 3.211818952894099 6 10 3.933078676272288 6 11 4.959494394021938 6 12 7.845516385145974 6 13 6.228425325942677 6 14 8.971547947142696 6 15 7.142685560221569 6 16 7.7953840565730665 6 17 6.672763211702218 6 18 6.1787512783319976 6 19 5.868272226834804 6 20 5.875368799892605 6 21 4.404355956623597 6 22 4.410903633041386 6 23 4.201485270906488 6 24 4.783524435205713 6 25 4.276835948199796 6 26 3.229868526974016 6 27 4.126550132505528 6 28 2.8496275501444615 6 29 4.251225337467417 6 30 4.577994872196989 6 31 3.971520066489809 6 32 5.023888157416408 6 33 3.2251673068532014 6 34 4.3406192028304105 6 35 3.624476018685449 6 36 3.9825060604552895 6 37 3.600541942157218 6 38 3.5713977433733426 6 39 5.526243992754665 6 40 4.318862700455348 6 41 -0.7966768651439544 6 42 7.494129681616034 6 43 10.755544612302156 6 44 9.953675242014718 6 45 7.2841257652826705 6 46 10.349772195596685 6 47 10.390763163942623 6 48 11.232609996034594 6 49 12.834523017656917 6 50 9.969094240060386 6 51 10.945510962080292 6 52 10.490070353434337 6 53 11.452300716069661 6 54 11.57300424205803 6 55 11.726556104382972 6 56 10.714864397983057 7 1 2.55968636305429 7 2 3.48867635946268 7 3 3.816094731505494 7 4 3.7266430459971005 7 5 4.4729287743384845 7 6 5.975419961512998 7 7 3.2407330157917755 7 8 3.4690677670460843 7 9 3.79894016501531 7 10 3.773514282332897 7 11 5.0783390909916335 7 12 6.858300476055067 7 13 7.505887447154802 7 14 8.739540371385118 7 15 6.78662495416096 7 16 6.394342389906402 7 17 6.905717757156765 7 18 6.461421732877451 7 19 5.469597984410562 7 20 5.71959228474109 7 21 3.578598380866019 7 22 3.9464149966777526 7 23 3.469004210300426 7 24 4.7058729200541976 7 25 3.4221957966846475 7 26 3.3382965572770473 7 27 3.462250890081286 7 28 3.747354822871735 7 29 4.183990488982566 7 30 3.697786538863653 7 31 3.1225617331564735 7 32 5.629475278628533 7 33 3.8425915492774436 7 34 3.8974373846485904 7 35 3.6604608671703005 7 36 1.9342106059098363 7 37 1.5110533057935847 7 38 1.9454507736763738 7 39 2.8860924776031496 7 40 -0.16598578439313627 7 41 -9.416942016659107 7 42 4.750758469494823 7 43 6.4217377941203395 7 44 6.33388357534805 7 45 4.345678795585702 7 46 5.552897195596685 7 47 5.8173730124274705 7 48 5.971246359670957 7 49 8.442004078262979 7 50 5.202048785514933 7 51 8.179885962080292 7 52 7.096604444343427 7 53 7.145482534251478 7 54 6.935693635997424 7 55 6.747389437716304 7 56 6.827553791922451 8 1 2.219794911222536 8 2 3.184515346346444 8 3 3.494068499031499 8 4 3.8996416891449996 8 5 4.577632528295972 8 6 5.983787216148912 8 7 3.340009361336776 8 8 3.4487149854992722 8 9 3.4002517887149963 8 10 4.137602930003631 8 11 5.418682826857761 8 12 8.185301832907168 8 13 7.85482457967402 8 14 8.99440242475464 8 15 8.027013918430523 8 16 7.889367265528289 8 17 7.054293062448487 8 18 6.327771800720054 8 19 6.01052968952137 8 20 6.51086320287768 8 21 4.012564911847477 8 22 4.709644304683174 8 23 4.238565494787082 8 24 5.1538602561012326 8 25 4.163263187005768 8 26 3.9572472583173024 8 27 4.062884087729408 8 28 3.288060385965359 8 29 3.629490262840555 8 30 3.5161944990626637 8 31 3.9782831261913003 8 32 5.441098978311928 8 33 3.824047903868127 8 34 4.223081889397577 8 35 4.117946167939181 8 36 0.44845755299260404 8 37 1.6175661958885605 8 38 1.579326847850954 8 39 3.2221395151427252 8 40 -1.9497940159625635 8 41 -11.019158208427536 8 42 5.880790129377228 8 43 7.642904686929022 8 44 7.202042779328149 8 45 4.613883227969239 8 46 8.366330031417583 8 47 8.267395626629192 8 48 8.264326413945042 8 49 11.112741301239005 8 50 7.805847971403669 8 51 10.560482977005663 8 52 10.363904308658217 8 53 10.685509671293541 8 54 10.593993048028178 8 55 10.22748894020387 8 56 8.672420368132308 9 1 2.0682090903270165 9 2 2.4692634806748046 9 3 3.287212155747916 9 4 2.723802136906194 9 5 3.94120528949 9 6 4.77040102211906 9 7 2.3141231673069242 9 8 2.3554314034097175 9 9 2.118068952894099 9 10 3.073703676272288 9 11 4.412619394021938 9 12 6.986141385145974 9 13 6.040925325942677 9 14 8.315297947142696 9 15 7.033310560221569 9 16 6.6078840565730665 9 17 6.422763211702218 9 18 5.6475012783319976 9 19 5.212022226834804 9 20 5.078493799892605 9 21 2.294980956623597 9 22 2.9577786330413858 9 23 3.1389852709064883 9 24 3.596024435205713 9 25 3.058085948199796 9 26 2.948618526974016 9 27 2.2671751325055283 9 28 1.7558775501444615 9 29 1.7356003374674174 9 30 3.5467448721969888 9 31 3.205895066489809 9 32 3.5551381574164083 9 33 2.9907923068532014 9 34 3.3718692028304105 9 35 2.499476018685449 9 36 1.5918810604552895 9 37 1.9130419421572178 9 38 1.1963977433733426 9 39 2.276243992754665 9 40 -0.21238729954465185 9 41 -6.984176865143954 9 42 6.025379681616034 9 43 8.083669612302156 9 44 7.906800242014718 9 45 5.5653757652826705 9 46 8.615397195596685 9 47 8.312638163942623 9 48 8.763859996034594 9 49 11.334523017656917 9 50 8.890969240060386 9 51 8.929885962080292 9 52 8.693195353434337 9 53 9.514800716069661 9 54 9.05737924205803 9 55 9.726556104382972 9 56 9.277364397983057 10 1 2.159840446259217 10 2 3.3641257688103963 10 3 3.833557494730968 10 4 3.6395860352112805 10 5 4.505029442032374 10 6 6.010602293305503 10 7 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2.807993526974016 115 27 1.5046751325055254 115 28 1.431770407287317 115 29 2.632475337467419 115 30 1.5025484436255567 115 31 1.8844664950612398 115 32 -6.620308271155022 115 33 1.3715958782817737 115 34 2.001333488544695 115 35 1.8834045901140186 115 36 3.788756060455288 115 37 3.9617026564429345 115 38 4.443719171944775 115 39 5.315529707040376 115 40 7.776005557598204 115 41 7.834573134856047 115 42 0.9200225387588894 115 43 1.411348183730727 115 44 1.1005502420147195 115 45 0.7153757652826691 115 46 -2.9185313758318863 115 47 -7.402540407485949 115 48 -2.9638185753939794 115 49 -8.07574483948594 115 50 -2.1719771885110433 115 51 -0.9201140379197099 115 52 -0.880018932279949 115 53 -0.7869849982160524 115 54 -0.815388615084828 115 55 -0.514515324188455 115 56 0.6621858265544844 116 1 1.3821971855651114 116 2 2.490840861627184 116 3 2.335575250986011 116 4 2.6508854702395297 116 5 2.940461241870949 116 6 2.1550736411666804 116 7 2.0589148339735885 116 8 2.2006694986478124 116 9 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49 -9.199247143633405 118 50 -7.214373501875098 118 51 -8.255597908887449 118 52 -8.05882077559792 118 53 -9.704957348446468 118 54 -14.95824575794197 118 55 -11.66658905690735 118 56 -11.276063021371781 119 1 0.7000025685878839 119 2 2.066410219805242 119 3 2.5881632427044394 119 4 2.5784217021235847 119 5 2.531558550359563 119 6 2.1012434134234113 119 7 0.24007425426344753 119 8 0.9315183599314594 119 9 -0.4783984384102524 119 10 1.2544101980114206 119 11 -11.814282779891105 119 12 -15.449999919201852 119 13 -4.593585543622542 119 14 -4.619484661552956 119 15 -2.498618787604517 119 16 -7.699860508644324 119 17 -11.70495417960213 119 18 -2.8409498086245257 119 19 -3.070586468817371 119 20 -2.613082287063918 119 21 -0.6934701303329263 119 22 0.08277863304138577 119 23 -1.100824511702207 119 24 -3.8007146952290682 119 25 -0.31759339962628985 119 26 0.5036457008870627 119 27 1.182936002070747 119 28 1.9746275501444615 119 29 2.07595359833698 119 30 0.5311198721969888 119 31 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120 51 -12.93301726372616 120 52 -12.833014323985017 120 53 -12.704957348446468 120 54 -13.829213499877454 120 55 -13.763363250455738 120 56 -11.630901731049201 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 565.0 5 40.0 6 73.0 7 69.0 8 79.0 9 68.0 10 45.0 11 51.0 12 55.0 13 62.0 14 134.0 15 163.0 16 226.0 17 318.0 18 412.0 19 468.0 20 518.0 21 695.0 22 894.0 23 1120.0 24 1180.0 25 1209.0 26 1326.0 27 1329.0 28 1388.0 29 1577.0 30 1761.0 31 2138.0 32 2556.0 33 3096.0 34 3624.0 35 4267.0 36 4895.0 37 4999.0 38 3168.0 39 662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.42516029184169 0.34932566880389126 0.17687375635640062 0.048640282998010174 8 99.65067433119611 0.276365244306876 0.033163829316825114 0.039796595180190145 9 98.75937106083725 0.7518963267652978 0.1592251044914748 0.3295075079059687 10 37.49281450364802 51.4105682069423 4.640725182401061 6.455892107008622 11 20.459871766526643 21.076718991819586 45.346009285872206 13.117399955781561 12 54.1034711474685 10.506301127570197 14.908246738890119 20.481980986071193 13 17.585673225735132 32.529294715896526 16.986513376077824 32.898518682290515 14 43.455763660084465 13.398049664993477 18.349070163412424 24.79711651150963 15 18.23214206633896 35.629511536247286 17.191444134449316 28.946902262964443 16 18.62794966494902 13.52367472410819 48.06599287878453 19.78238273215826 17 48.87665295652558 17.619742603157757 15.284595993100702 18.219008447215955 18 49.466003316749585 18.62244333886125 13.86180210060807 18.049751243781095 19 19.860696517412933 47.25925925925926 15.002763957987838 17.87728026533997 20 18.792020523708423 19.815994338287332 13.944179051663127 47.44780608634112 21 20.00398001149781 17.478883827886612 44.39923937558042 18.117896785035157 22 19.24607561353084 20.070749502542558 31.828432456334294 28.854742427592306 23 22.668582799027195 46.93345124917091 15.414547866460316 14.983418085341588 24 17.755914216228167 48.693345124917094 16.263541896971038 17.287198761883705 25 20.019898297590093 49.597612204289184 14.952465177979219 15.4300243201415 26 33.77704932556645 21.034795898026534 29.504529446942346 15.683625329464663 27 46.3285707966559 17.005794665369134 18.445614190295043 18.22002034767992 28 18.715949860477476 46.70018160074412 18.915267750365416 15.668600788412986 29 17.631968862646232 46.72814524867866 20.24370286826334 15.396183020411774 30 16.075101174285145 20.358698777062738 49.337667794511155 14.228532254140958 31 17.41924048389241 19.548455709664555 49.18465015287809 13.84765365356494 32 43.37799593172371 17.681524719200496 22.79782435659326 16.142654992482534 33 16.65854522902454 45.098343226721006 23.414104722246833 14.82900682200762 34 15.217968577118832 20.061960610754593 25.768975437043594 38.951095375082986 35 42.34078793107262 19.92169354303537 25.883159687658992 11.854358838233017 36 41.93898335104499 20.514523556500176 24.97343251859724 12.573060573857598 37 40.288583017487866 20.741627324511825 26.45344326972095 12.516346388279361 38 17.50105174589819 21.287336979385778 49.3257755242123 11.885835750503732 39 14.792216724723215 39.42668234563244 22.480086973885648 23.30101395575869 40 10.01890359168242 14.978316468364284 19.363949738685644 55.63883020126765 41 28.577479546113203 15.585081481151214 21.16726486390084 34.670174108834736 42 13.989407671682141 23.50699137988344 26.099674252664702 36.40392669576972 43 9.014884319336305 24.699984472394135 21.306093476187307 44.97903773208225 44 9.638019098378859 14.807905840550745 19.671330224294913 55.88274483677548 45 13.925479864508844 45.885452412052516 26.31229382983905 13.876773893599593 46 39.120815911152405 20.92652817415544 27.51709032985996 12.435565584832194 47 13.264335788053694 18.34184745350406 48.48183285786948 19.911983900572768 48 13.607713912908862 21.956343852975653 51.177654422009425 13.258287812106067 49 9.829959873190413 14.274946238942956 20.23854390671071 55.65654998115592 50 12.895220791881773 20.255688742162054 53.64367535949306 13.20541510646312 51 39.5856439377135 21.88456590213389 26.267689987134556 12.262100173018057 52 13.644938633286726 47.26013715959784 25.64307432807333 13.451849879042102 53 37.62323592618029 23.009947493187408 26.38633493586193 12.980481644770366 54 12.23324183122288 20.06995483928097 45.28247587000797 22.414327459488177 55 14.581810934697403 21.165216235966252 27.569255297947254 36.68371753138909 56 39.63367367278687 21.44360036322562 25.204314411640937 13.718411552346572 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.5 11 1.0 12 15.5 13 71.0 14 112.0 15 155.5 16 199.0 17 223.0 18 247.0 19 293.0 20 339.0 21 326.0 22 362.5 23 412.0 24 490.0 25 568.0 26 642.5 27 717.0 28 847.5 29 1118.5 30 1259.0 31 1345.0 32 1431.0 33 1365.5 34 1300.0 35 1178.0 36 1056.0 37 993.0 38 854.5 39 779.0 40 742.5 41 706.0 42 717.0 43 728.0 44 753.0 45 778.0 46 1036.0 47 2004.0 48 2714.0 49 3370.5 50 4027.0 51 3849.5 52 3672.0 53 3233.5 54 2659.5 55 2524.0 56 2544.0 57 2564.0 58 2369.0 59 2174.0 60 2061.0 61 1948.0 62 1933.5 63 1855.0 64 1791.0 65 1675.5 66 1560.0 67 1309.0 68 1058.0 69 948.5 70 839.0 71 699.0 72 482.0 73 405.0 74 323.0 75 241.0 76 198.5 77 156.0 78 107.5 79 46.5 80 34.0 81 20.5 82 7.0 83 5.0 84 3.0 85 2.5 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.024320141499005087 10 0.0 11 0.0 12 0.0 13 0.0 14 0.015476453681185052 15 0.15034269290294053 16 0.028741985407915102 17 0.017687375635640063 18 0.011054609772275039 19 0.011054609772275039 20 0.030952907362370104 21 0.008843687817820032 22 0.0 23 0.0 24 0.0 25 0.0 26 0.17908467831085564 27 0.035374751271280126 28 0.16803006853858057 29 0.024320141499005087 30 0.024320141499005087 31 0.21224850762768072 32 0.004421843908910016 33 0.18129560026531064 34 0.08843687817820031 35 0.05085120495246517 36 0.13707716117621047 37 0.24983418085341588 38 0.1481317709484855 39 0.3515365907583462 40 0.5858943179305771 41 0.824673889011718 42 0.22772496130886577 43 0.3294273712137961 44 0.4421843908910016 45 0.13486623922175547 46 0.06411673667919522 47 0.024320141499005087 48 0.028741985407915102 49 0.27194340039796594 50 0.21445942958213576 51 0.3272164492593411 52 0.38248949812071636 53 0.20561574176431574 54 0.12823347335839044 55 0.15697545876630553 56 0.17466283440194563 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 45230.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.72186601812956 #Duplication Level Percentage of deduplicated Percentage of total 1 92.84066688078484 70.30068538580588 2 4.511080615492423 6.831748839265973 3 1.1854360711261642 2.6929029405261993 4 0.4292096119594733 1.3000221092195445 5 0.28613974130631553 1.0833517576829539 6 0.15766883704633713 0.7163387132434226 7 0.10219276475225555 0.5416758788414769 8 0.07883441852316857 0.4775591421622817 9 0.0321177260649946 0.21888127349104577 >10 0.30657829425676664 4.479327879725846 >50 0.02335834622908698 1.1673667919522441 >100 0.04671669245817396 10.190139288083131 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 433 0.9573292062790183 No Hit CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 399 0.8821578598275481 No Hit CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 399 0.8821578598275481 No Hit CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 393 0.868892328100818 No Hit CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 391 0.8644704841919081 No Hit CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 374 0.8268848109661728 No Hit CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 351 0.7760336060137077 No Hit CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 312 0.6898076497899623 No Hit CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 280 0.6190581472474022 No Hit CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 262 0.579261552067212 No Hit CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 240 0.5306212690692018 No Hit CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 238 0.5261994251602918 No Hit CGAGTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 174 0.38470042007517136 No Hit CGAGTAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 131 0.289630776033606 No Hit CGAGTAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 121 0.26752155648905596 No Hit CGAGTAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 111 0.24541233694450587 No Hit CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTAACCACAGTTCTGAGTCG 75 0.16581914658412558 No Hit CGAGTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 74 0.16360822462967056 No Hit CGAGTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 72 0.15918638072076058 No Hit CGAGTAGGGATGCGCTGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 71 0.15697545876630553 No Hit CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACTCACAGTTCTGAGTCG 67 0.1481317709484855 No Hit CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAACCACAGTTCTGAGTCG 65 0.14370992703957552 No Hit CGAGTAGGGATGCGCTGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 52 0.11496794163166041 No Hit CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACTCACAGTTCTGAGTCG 52 0.11496794163166041 No Hit CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACTCACAGTTCTGAGTCG 49 0.10833517576829539 No Hit CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTAACCACAGTTCTGAGTCG 48 0.10612425381384036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.004421843908910016 0.0 0.0 0.0 0.0 7 0.004421843908910016 0.0 0.0 0.0 0.0 8 0.004421843908910016 0.0 0.0 0.0 0.0 9 0.006632765863365023 0.0 0.0 0.0 0.0 10 0.006632765863365023 0.0 0.0 0.0 0.0 11 0.011054609772275039 0.0 0.0 0.0 0.0 12 0.011054609772275039 0.0 0.0 0.0 0.0 13 0.017687375635640063 0.0 0.0 0.0 0.0 14 0.01989829759009507 0.0 0.0 0.0 0.0 15 0.01989829759009507 0.0 0.0 0.0 0.0 16 0.024320141499005084 0.0 0.0 0.0 0.0 17 0.024320141499005084 0.0 0.0 0.0 0.0 18 0.026531063453460093 0.0 0.0 0.0 0.0 19 0.033163829316825114 0.0 0.0 0.0 0.0 20 0.035374751271280126 0.0 0.0 0.0 0.0 21 0.03979659518019014 0.0 0.0 0.0 0.0 22 0.044218439089100156 0.0 0.0 0.0 0.0 23 0.053062126906920186 0.0 0.0 0.0 0.0 24 0.05527304886137519 0.0 0.0 0.0 0.0 25 0.061905814724740216 0.0 0.0 0.0 0.0 26 0.07074950254256025 0.0 0.0 0.0 0.0 27 0.07517134645147026 0.0 0.0 0.0 0.0 28 0.0840150342692903 0.0 0.0 0.0 0.0 29 0.0840150342692903 0.0 0.0 0.0 0.0 30 0.0840150342692903 0.0 0.0 0.0 0.0 31 0.0862259562237453 0.0 0.0 0.0 0.0 32 0.09064780013265532 0.0 0.0 0.0 0.0 33 0.09064780013265532 0.0 0.0 0.0 0.0 34 0.09064780013265532 0.0 0.0 0.0 0.0 35 0.09064780013265532 0.0 0.0 0.0 0.0 36 0.09064780013265532 0.0 0.0 0.0 0.0 37 0.09064780013265532 0.0 0.0 0.0 0.0 38 0.09064780013265532 0.0 0.0 0.0 0.0 39 0.09064780013265532 0.0 0.0 0.0 0.0 40 0.09064780013265532 0.0 0.0 0.0 0.0 41 0.09285872208711032 0.0 0.0 0.0 0.0 42 0.09506964404156533 0.0 0.0 0.0 0.0 43 0.10170240990493036 0.0 0.0 0.0 0.0 44 0.10833517576829538 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCAAG 35 4.6373316E-8 50.653156 40 TACACAC 35 4.6373316E-8 50.653156 38 ACACAGT 45 1.1459633E-10 50.653156 41 TAACCAC 35 4.6373316E-8 50.653156 38 TATCCAC 20 3.883418E-4 50.653156 38 AACTAAA 20 3.883418E-4 50.653156 38 CCACAGT 270 0.0 50.653156 41 GCCAAGT 35 4.6373316E-8 50.653156 41 TCACAGT 20 3.883418E-4 50.653156 41 TACTCAC 20 3.883418E-4 50.653156 38 ACGCCTA 15 0.00795911 50.653152 39 CCAACCC 15 0.00795911 50.653152 39 AACAACC 15 0.00795911 50.653152 38 CGCCTAG 15 0.00795911 50.653152 40 CAACCCA 15 0.00795911 50.653152 40 CCGCCCA 100 0.0 50.653152 39 ACCAACC 25 1.9030522E-5 50.653152 38 TACGCCT 15 0.00795911 50.653152 38 TCCGCCC 110 0.0 50.653152 38 TACTTTT 15 0.00795911 50.653152 39 >>END_MODULE