FastQCFastQC Report
Fri 17 Jun 2016
SRR1526357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62280
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1410.22639691714836221No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1400.22479126525369297No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1340.2151573538856776No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC920.1477199743095697No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC860.13808606294155426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGACC203.9242854E-450.5766738
ACGCCCA309.531377E-750.5766739
TACGCCC309.531377E-750.5766738
AAACCAC150.00804720850.53545437
CAACCTG251.9475601E-550.494341
ATATGCT150.008230424550.24878334
TAGAGCC451.2551027E-1050.24878334
TAAAGGC150.008230424550.24878334
CTCTGAG150.00836318150.0460147
TATATTT150.00836318150.0460146
AACGATT150.00836318150.0460147
CTTATAT150.00836318150.0460144
ACGATTA150.00836318150.0460148
TCTCCAG150.00836318150.0460146
TCACAAC150.00836318150.0460146
CAACGAT150.00836318150.0460146
CGATTAA150.00836318150.0460149
ACGGGTA402.6102498E-950.00564633
GTGTCTA150.00844359649.92512523
AACCGTG150.00844359649.92512525
Produced by FastQC (version 0.11.5)