##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62280
Sequences flagged as poor quality	0
Sequence length	56
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.1599710982659	39.0	37.0	39.0	33.0	40.0
2	36.44741490044958	39.0	36.0	39.0	30.0	40.0
3	36.723089274245346	39.0	37.0	39.0	30.0	40.0
4	34.359344894026975	38.0	35.0	39.0	17.0	40.0
5	34.68431278098908	39.0	35.0	39.0	17.0	40.0
6	36.463615928066794	38.0	36.0	39.0	30.0	40.0
7	36.7147880539499	39.0	36.0	39.0	30.0	40.0
8	36.386319845857415	38.0	36.0	39.0	30.0	40.0
9	36.28363840719332	38.0	36.0	39.0	29.0	40.0
10	36.101204238921	38.0	35.0	39.0	29.0	40.0
11	35.49799293513166	38.0	35.0	39.0	29.0	40.0
12	33.428147077713554	38.0	31.0	39.0	20.0	40.0
13	32.79873153500321	37.0	31.0	39.0	18.0	40.0
14	32.41968529222864	37.0	29.0	39.0	16.0	40.0
15	33.01295761078998	37.0	31.0	39.0	18.0	40.0
16	32.71359987154785	36.0	30.0	39.0	19.0	40.0
17	32.618962748876044	36.0	30.0	38.0	18.0	40.0
18	32.71864161849711	36.0	30.0	38.0	20.0	40.0
19	33.67291265253693	36.0	31.0	38.0	25.0	40.0
20	34.01581567116249	37.0	32.0	39.0	25.0	40.0
21	35.37103403982017	38.0	35.0	39.0	28.0	40.0
22	34.99471740526654	38.0	35.0	39.0	26.0	40.0
23	35.235228002569045	38.0	35.0	39.0	27.0	40.0
24	34.86665061014772	38.0	34.0	39.0	26.0	40.0
25	35.170070648683364	38.0	35.0	39.0	27.0	40.0
26	35.500016056518945	38.0	35.0	39.0	29.0	40.0
27	35.43595054592164	38.0	35.0	39.0	28.0	40.0
28	35.400786769428386	38.0	35.0	39.0	29.0	40.0
29	35.25563583815029	38.0	35.0	39.0	28.0	40.0
30	35.0754816955684	38.0	35.0	39.0	27.0	40.0
31	34.81624919717405	38.0	35.0	39.0	27.0	40.0
32	33.764900449582534	38.0	33.0	39.0	19.0	40.0
33	35.10553949903661	38.0	35.0	39.0	28.0	40.0
34	34.86461143224149	38.0	35.0	39.0	27.0	40.0
35	34.87003853564547	38.0	35.0	39.0	27.0	40.0
36	32.86860950545922	36.0	31.0	38.0	24.0	39.0
37	32.62988118175979	36.0	31.0	38.0	23.0	39.0
38	31.812202954399485	35.0	31.0	38.0	17.0	39.0
39	29.76655427103404	34.0	27.0	38.0	7.0	39.0
40	25.483702633269107	31.0	12.0	38.0	4.0	39.0
41	22.260163776493258	26.0	9.0	33.0	4.0	37.0
42	26.19145793192036	30.0	22.0	35.0	4.0	38.0
43	22.51029222864483	27.0	9.0	33.0	4.0	37.0
44	23.49460500963391	28.0	10.0	34.0	4.0	37.0
45	25.990269749518305	30.0	22.0	35.0	4.0	38.0
46	26.03747591522158	31.0	18.0	37.0	4.0	38.0
47	25.196547848426462	30.0	12.0	36.0	4.0	38.0
48	25.138086062941554	30.0	13.0	36.0	4.0	38.0
49	22.588021836865767	27.0	6.0	35.0	4.0	38.0
50	25.004977520873474	30.0	13.0	36.0	4.0	38.0
51	26.44009312780989	33.0	7.0	38.0	4.0	39.0
52	26.252103403982016	33.0	4.0	38.0	4.0	39.0
53	25.823217726396916	32.0	4.0	38.0	4.0	39.0
54	24.89229287090559	32.0	4.0	38.0	4.0	39.0
55	25.123378291586384	32.0	4.0	38.0	4.0	39.0
56	23.972077713551702	31.0	4.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.428410319624298
1	2	2.093740894853319
1	3	1.9808161035342025
1	4	4.6980759974788455
1	5	4.35085571488942
1	6	1.8867218755103963
1	7	2.2423650494800995
1	8	2.41102434940143
1	9	2.5241214488590913
1	10	2.5660200777818005
1	11	-3.108724398617472
1	12	-6.628582438305788
1	13	-20.909630676395082
1	14	-18.051317611205032
1	15	-10.885454668409196
1	16	-20.47239181667147
1	17	-20.207157820485328
1	18	-19.848040327441186
1	19	-5.387711360616997
1	20	-10.456142730839382
1	21	-6.041015625001574
1	22	-2.25705226435991
1	23	-3.4269081647437254
1	24	-3.2108518076030457
1	25	-2.3746355193360067
1	26	2.0299635316243894
1	27	1.6918257541850394
1	28	2.069323992126236
1	29	1.5746616883377342
1	30	2.6603455225970407
1	31	1.3794020405474114
1	32	-11.085735197604581
1	33	0.6457301744926411
1	34	0.3194837699495423
1	35	1.33684979763364
1	36	3.6662136817641446
1	37	3.2293448122613597
1	38	4.291455510328589
1	39	6.176540629155642
1	40	9.181718662326837
1	41	-0.053955950686326304
1	42	-4.8617564342982185
1	43	-14.480759557341614
1	44	-16.554544433454453
1	45	-14.75171884226383
1	46	-18.21888109474843
1	47	-18.098993533843576
1	48	-16.179802321813643
1	49	-13.890287951278278
1	50	-15.967373152875886
1	51	-16.705351354667375
1	52	-17.068270729036545
1	53	-16.84092488245178
1	54	-18.17004335684183
1	55	-16.75575728148943
1	56	-15.53212743598656
2	1	1.0600459006859921
2	2	1.8505558159437072
2	3	1.7053498194595988
2	4	4.231074562758614
2	5	4.200927450900899
2	6	1.3418868683368004
2	7	1.832393743884687
2	8	2.0555724125291235
2	9	2.073977976836133
2	10	1.7152309816555444
2	11	-3.825367153280311
2	12	-11.371050157100623
2	13	-14.652457075247309
2	14	-16.673268830717227
2	15	-8.568740177734878
2	16	-3.6463516445050423
2	17	-2.452494979739278
2	18	-10.704209624428273
2	19	-2.1829050478479886
2	20	-3.6580796031492824
2	21	2.0228294252136365
2	22	2.768772699772086
2	23	2.0734505153136666
2	24	2.032691951363958
2	25	1.7867705064889563
2	26	1.9338372762441907
2	27	2.0713092549024026
2	28	1.4172436477933772
2	29	2.3070863655256844
2	30	2.3637171151364953
2	31	-1.9484315319059569
2	32	-12.088604638063693
2	33	-1.50886093024193
2	34	-1.4764272773962261
2	35	-1.5688173472731037
2	36	4.2279066516350206
2	37	3.7652128180002364
2	38	5.49339238263849
2	39	7.710256554550195
2	40	10.680283942097276
2	41	7.483705455339496
2	42	3.992978142459311
2	43	-0.900056544429134
2	44	-9.160355050384151
2	45	2.7281950745223966
2	46	-10.803888268349576
2	47	-11.08249425120369
2	48	-7.601968749360271
2	49	-11.919341035926774
2	50	-6.682581187309172
2	51	-5.8481060175081225
2	52	-5.891441460743863
2	53	-6.933464337258091
2	54	-15.073917101461628
2	55	-7.583949534000189
2	56	-6.395829014178812
3	1	2.09331608775679
3	2	2.98837253391671
3	3	2.699548256589601
3	4	5.147247899249265
3	5	4.809736354523913
3	6	2.5062250165726496
3	7	2.936111480091178
3	8	3.1647365138788714
3	9	3.1913116259007808
3	10	2.926100032093622
3	11	1.9455303129759045
3	12	3.1267002572967257
3	13	-3.3929259362465984
3	14	-2.546463242272992
3	15	-1.4686928180379795
3	16	3.176523089096662
3	17	3.34823909556264
3	18	3.0353120426330555
3	19	2.2966118832436564
3	20	1.7681576689321759
3	21	3.7219769506694718
3	22	4.114735285611538
3	23	3.1029319266326283
3	24	3.885521693253608
3	25	3.84952210487873
3	26	2.654178266061855
3	27	3.068467673088527
3	28	2.567610685444336
3	29	3.2071574050710296
3	30	2.6767647116318756
3	31	2.833713862363517
3	32	3.2095246539088365
3	33	2.818631373807321
3	34	3.235388966979812
3	35	2.8644054801007997
3	36	5.167641437332396
3	37	5.5531597751397115
3	38	6.309873557158973
3	39	8.661120869018578
3	40	11.620610724005878
3	41	11.77828277004468
3	42	10.06271529614153
3	43	4.81093090525119
3	44	7.618356765860224
3	45	10.198595263961185
3	46	3.9954250160454023
3	47	-0.8428541849001157
3	48	3.4702547884090897
3	49	-8.613160595481592
3	50	3.0557564873297096
3	51	1.2144087823971574
3	52	1.3176516010605432
3	53	0.8632441638075008
3	54	-6.221442557298712
3	55	0.29978241011536966
3	56	3.952738354990025
4	1	2.0579352065021226
4	2	2.7888766414596518
4	3	2.5006124836246997
4	4	5.674320341370247
4	5	5.202100058780822
4	6	2.532083866460624
4	7	2.9578627870366603
4	8	3.2260695982702075
4	9	2.9466327610762875
4	10	2.8273322949763653
4	11	3.2006647416540233
4	12	3.3001505933684214
4	13	5.666726789668267
4	14	2.650605465718048
4	15	5.877554381364558
4	16	6.192766554369257
4	17	5.691032224763539
4	18	5.243023020975105
4	19	4.693736603183908
4	20	4.079490753323512
4	21	2.8549119768083813
4	22	3.450526921160453
4	23	3.0504674008671344
4	24	3.9271572421707077
4	25	3.1751382363201017
4	26	2.4875427171447555
4	27	2.793635708936172
4	28	2.2101610961986324
4	29	2.558258973405607
4	30	2.7842143008775793
4	31	2.2600581491446974
4	32	3.9029525852008504
4	33	2.3680152423659493
4	34	2.1481036794518005
4	35	2.4539312455974382
4	36	3.5344263514474044
4	37	4.784209065655027
4	38	4.2173604877041555
4	39	7.722920719653384
4	40	10.156831784498777
4	41	7.31086305383856
4	42	8.118447185611284
4	43	9.697973474332592
4	44	11.715817557495772
4	45	9.197941791220334
4	46	7.912340624708584
4	47	8.542974791948279
4	48	8.256849261896765
4	49	5.865368157319004
4	50	8.011133634454431
4	51	7.885711993748913
4	52	6.672113886348072
4	53	6.8038714976387205
4	54	1.910838996099347
4	55	6.474446338420073
4	56	5.668664419217059
5	1	1.923704437271354
5	2	2.8007997183827342
5	3	2.5102278682400865
5	4	5.302781879831791
5	5	4.865561597242362
5	6	2.595545404922163
5	7	2.4782474024212746
5	8	2.863377290577901
5	9	2.434709684153205
5	10	2.4042553718994455
5	11	2.869510895500177
5	12	5.695535208753036
5	13	5.830957558899037
5	14	5.788682388794971
5	15	6.191015919826096
5	16	6.085843477446176
5	17	6.138724532455846
5	18	5.64725379020587
5	19	4.2475827570300595
5	20	4.286798445631206
5	21	2.5172196691160735
5	22	2.6670653826989152
5	23	2.0166212470209786
5	24	2.745618780632249
5	25	2.29359977478164
5	26	1.3940811786832157
5	27	2.3059434012438658
5	28	1.0128534038909365
5	29	1.3117205118671507
5	30	1.8615219931852707
5	31	2.1335196876062383
5	32	3.056029508277767
5	33	1.624553703904411
5	34	1.2777190640671847
5	35	2.0750850917512835
5	36	2.9532725052935582
5	37	3.7822859887319495
5	38	3.668514333857999
5	39	6.768305335037997
5	40	4.807601015268013
5	41	3.116632284607789
5	42	7.678831800995898
5	43	11.50335808971721
5	44	10.674279095957314
5	45	8.620634098912642
5	46	8.814648317016278
5	47	9.433359407332894
5	48	9.826080031127532
5	49	10.163829695780546
5	50	8.984979788300585
5	51	8.25994276297968
5	52	8.881729270963458
5	53	8.408486882254103
5	54	8.175838996099348
5	55	8.234830953804689
5	56	8.069049034601676
6	1	2.1611791847461035
6	2	3.0084764860595
6	3	2.4647733227855397
6	4	5.195105112155019
6	5	4.655965637646403
6	6	2.660191869568628
6	7	2.9356211397950105
6	8	2.917114664315278
6	9	3.259861199304723
6	10	2.9471846648287396
6	11	3.681531097520377
6	12	6.1352321784500035
6	13	6.558432306373781
6	14	6.039591479704061
6	15	6.470308849119025
6	16	6.835540447143146
6	17	6.089633623364939
6	18	5.627152780104861
6	19	4.738996898444199
6	20	4.783162081994838
6	21	3.3047954266918325
6	22	3.885550231183764
6	23	3.0809646813644136
6	24	3.6600632250766907
6	25	2.5519836131654827
6	26	2.5968084514104888
6	27	2.60715552245599
6	28	2.574671585709119
6	29	2.661114451261085
6	30	3.568693710356989
6	31	3.2846307987173518
6	32	4.265423447671708
6	33	3.3282910776417864
6	34	3.2486281549762808
6	35	2.8417517584179492
6	36	3.37387856589962
6	37	3.0974375038834623
6	38	4.153160798504466
6	39	6.391335638068302
6	40	2.8825505102175057
6	41	1.9545110724865786
6	42	6.535599477763576
6	43	11.649216675575794
6	44	9.996703338381554
6	45	7.11043207871062
6	46	9.900102862470824
6	47	10.75810688208037
6	48	10.265979021026524
6	49	11.97201151396236
6	50	10.384676757997553
6	51	9.221861954898873
6	52	8.929204018438202
6	53	8.435961629728851
6	54	9.020687480947831
6	55	9.313315802289537
6	56	10.065614691167333
7	1	2.1583852883351824
7	2	3.006757165191246
7	3	2.797461910793281
7	4	5.452143581959447
7	5	5.338965852561508
7	6	2.6210773198157824
7	7	2.9322899556127595
7	8	3.1178453756842828
7	9	3.148965003302145
7	10	3.0053192016866817
7	11	3.649723661457621
7	12	5.782556485348778
7	13	6.289680963154353
7	14	6.661448346241773
7	15	6.211228685783542
7	16	6.461588158297239
7	17	6.78595857500904
7	18	5.350019747652677
7	19	5.438008288944957
7	20	4.81701121158865
7	21	3.25998562656288
7	22	3.5264270848265724
7	23	2.9976850768082173
7	24	3.375193248717352
7	25	2.7880678598880237
7	26	2.7921662850661946
7	27	2.1774327629459904
7	28	2.7651938294228557
7	29	2.5727843416543834
7	30	3.2345007165895296
7	31	3.2692643684573
7	32	4.889859295511812
7	33	2.7630643422022843
7	34	3.604527574705486
7	35	3.334659559836389
7	36	1.8558256967829188
7	37	2.4971796057532245
7	38	2.752344121092044
7	39	4.8968159733358725
7	40	-2.569845793242628
7	41	-10.407835800498594
7	42	5.401384992485259
7	43	9.797400642908698
7	44	8.722364202340291
7	45	5.36467665210413
7	46	8.18443555105883
7	47	8.772721109460555
7	48	9.116505563042427
7	49	11.185744589397565
7	50	9.053277660641012
7	51	10.019304465107343
7	52	10.649814377346434
7	53	10.965082626934958
7	54	11.386902825886583
7	55	10.49674584742171
7	56	10.738836268644231
8	1	2.0648672279690317
8	2	2.9268462300106393
8	3	2.7079022868447353
8	4	5.346502810064344
8	5	5.266724387940037
8	6	2.6955454049221643
8	7	3.0935962396305783
8	8	3.148028453368596
8	9	3.4656399167113463
8	10	2.363557697480843
8	11	3.444627174569945
8	12	5.944604976194896
8	13	6.473748256573451
8	14	6.971938202748458
8	15	6.541015919826098
8	16	6.830959756515945
8	17	6.556864067339568
8	18	6.172137511136103
8	19	5.120838570983551
8	20	4.6119147247009735
8	21	3.035591762139333
8	22	3.862646778047747
8	23	3.146621247020981
8	24	3.436549013190387
8	25	3.5856927980374564
8	26	2.8705928065901887
8	27	2.997571308220607
8	28	2.974248752728151
8	29	3.5821856281462203
8	30	2.7701266443480606
8	31	3.801426664350423
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115	17	-7.563775467544154
115	18	-3.3835795431274605
115	19	-3.131583909636607
115	20	-1.999034887702127
115	21	0.25555300244941037
115	22	0.05789871603224839
115	23	-0.040878752979018884
115	24	1.2364521139655835
115	25	0.2760997747816418
115	26	1.6365811786832154
115	27	1.3401100679105298
115	28	1.6011867372242747
115	29	1.992553845200483
115	30	2.2890219931852727
115	31	1.8610196876062375
115	32	-3.92397049172223
115	33	1.2462203705710735
115	34	1.5118857307338516
115	35	1.5709184250846207
115	36	4.334105838626893
115	37	4.707285988731947
115	38	5.751014333858002
115	39	7.379972001704662
115	40	7.512601015268011
115	41	8.249965617941122
115	42	3.3588318009958975
115	43	1.2508580897172088
115	44	-0.26655423737602035
115	45	3.5264674322459726
115	46	-1.1870183496503905
115	47	-7.008307259333771
115	48	-3.3072533022057975
115	49	-7.450336970886122
115	50	0.6024797883005846
115	51	3.342442762979683
115	52	3.515062604296787
115	53	2.771820215587436
115	54	3.119172329432679
115	55	4.5273309538046895
115	56	6.096549034601676
116	1	1.2983198218867429
116	2	1.8177227953058122
116	3	1.8756124836246997
116	4	3.3089357259856342
116	5	3.5001769818577486
116	6	1.791699251076011
116	7	2.04440124857512
116	8	2.2741465213471344
116	9	2.3600942995378205
116	10	0.923486141130212
116	11	-0.23202756603828334
116	12	-0.9883109450931187
116	13	-5.708273210331736
116	14	-8.032086841974262
116	15	-6.651291772481596
116	16	-7.422618061015363
116	17	-8.424352390621078
116	18	-3.170438517486435
116	19	-4.517801858354556
116	20	-4.160893862061101
116	21	0.9895273614237681
116	22	2.056296151929679
116	23	0.3004674008671344
116	24	1.542541857555328
116	25	0.34821515939702863
116	26	2.054850409452449
116	27	2.293635708936172
116	28	1.0659303269678588
116	29	1.894797434944067
116	30	2.293829685492966
116	31	0.4235196876062375
116	32	-6.943201260953
116	33	1.166092165442869
116	34	1.330795987144107
116	35	1.0404697071358981
116	36	3.8517340437550978
116	37	3.4284398348857934
116	38	4.457745103088769
116	39	7.145997642730304
116	40	9.464524092191091
116	41	5.820478438453943
116	42	2.6280625702266676
116	43	-10.167411141052021
116	44	0.004279095957311796
116	45	1.2652494835280237
116	46	-5.337659375291416
116	47	-8.466640592667105
116	48	-8.666227661180157
116	49	-7.057708765757917
116	50	-3.9696355963147987
116	51	-0.4123649293280103
116	52	-0.7221168828826983
116	53	-2.388436194668973
116	54	-6.829545619285268
116	55	-3.362092123118387
116	56	-1.3024894269367842
117	1	-0.46508344151651926
117	2	0.02867850626152091
117	3	-0.1715903135780934
117	4	1.9021758192257252
117	5	1.3125312942120573
117	6	-0.10748489810814021
117	7	0.28915649333036697
117	8	0.3312560784566898
117	9	0.7144066538501761
117	10	0.2300129476570234
117	11	-9.672004256014976
117	12	-12.955676912459086
117	13	-7.582981835040361
117	14	-8.021014580902001
117	15	-7.065044686234508
117	16	-11.255368643765944
117	17	-10.940669406938092
117	18	-4.514261361309281
117	19	-4.250902091454787
117	20	-4.580474281641521
117	21	-2.2406591187627143
117	22	-1.114449768816236
117	23	-1.4139848135850812
117	24	-6.905593340579873
117	25	-1.4076123464304828
117	26	-0.03955518495315147
117	27	-0.48375356845310336
117	28	-0.18290417186663888
117	29	0.23687202701866283
117	30	0.3363704780337571
117	31	-2.0689045548180047
117	32	-7.916394734146472
117	33	-0.9242341748834662
117	34	-0.902886996538875
117	35	-1.8855209688547774
117	36	-0.2321820401609891
117	37	-0.14498673854077992
117	38	0.2945749399186077
117	39	2.1792144259470874
117	40	2.387601015268011
117	41	3.439359557335063
117	42	0.07095301311710855
117	43	-6.785126758767639
117	44	-2.245720904042688
117	45	0.8882098564883982
117	46	-4.099897137529176
117	47	-6.746943622970136
117	48	-3.451192696145192
117	49	-7.088594546643698
117	50	-2.9486565753357787
117	51	-0.9700572370203169
117	52	-1.0000889108547284
117	53	-2.4933312995640797
117	54	-5.949009488749137
117	55	-2.959411470437736
117	56	0.4999581255107657
118	1	1.2803711039380232
118	2	2.174133051716062
118	3	1.8991167571289722
118	4	4.229448546498453
118	5	4.037783819464586
118	6	1.5511009604777186
118	7	2.0649140690879406
118	8	2.1433772905779023
118	9	2.3002652397087644
118	10	2.2098109274550026
118	11	-5.698266882277601
118	12	-8.99002034680252
118	13	-7.277931329989855
118	14	-0.8513176112050331
118	15	-15.570095191285013
118	16	-5.778600966998269
118	17	-9.512386578655267
118	18	-2.3849684320163504
118	19	-3.091306131858829
118	20	-3.2976459988132376
118	21	2.2583307802271833
118	22	2.081509827143357
118	23	1.8021768025765397
118	24	-2.3399367749233093
118	25	1.6913775525594161
118	26	1.5463034009054368
118	27	2.508165623466091
118	28	1.2372978483353805
118	29	2.121720511867146
118	30	2.2292997709630527
118	31	0.6624085764951246
118	32	-8.651748269500008
118	33	1.0656648150155235
118	34	1.735496841844963
118	35	1.241751758417955
118	36	3.846605838626889
118	37	2.8933970998430567
118	38	3.577403222746888
118	39	6.243860890593556
118	40	9.809823237490235
118	41	7.211076729052234
118	42	1.8588318009958975
118	43	0.13002475638387523
118	44	0.5987235404017568
118	45	3.4639674322459726
118	46	-0.8897961274281663
118	47	-8.405529481555993
118	48	-5.812808857761354
118	49	-4.379503637552789
118	50	-0.07390910058830258
118	51	0.34105387409079313
118	52	0.4261737154078986
118	53	-1.6448464510792284
118	54	-6.064161003900654
118	55	-2.7129468239730876
118	56	0.7201601457127857
119	1	1.0637044372713547
119	2	1.540799718382729
119	3	1.5602278682400836
119	4	3.5377818798317904
119	5	3.2155615972423632
119	6	1.2705454049221672
119	7	1.6982474024212735
119	8	1.8683772905779037
119	9	2.19470968415321
119	10	0.7292553718994483
119	11	-10.095489104499823
119	12	-11.509464791246966
119	13	-1.8640424411009668
119	14	-2.5013176112050317
119	15	-6.558984080173904
119	16	-8.909156522553822
119	17	-9.826275467544153
119	18	-2.1877462097941276
119	19	-1.527417242969939
119	20	-1.803201554368794
119	21	-0.027780330883928173
119	22	-0.03793461730109016
119	23	-1.0783787529790203
119	24	-5.2343812193677515
119	25	-1.4614002252183553
119	26	0.474081178683214
119	27	1.4359434012438683
119	28	1.1928534038909362
119	29	0.7467205118671458
119	30	1.1515219931852698
119	31	-0.42648031239376394
119	32	-7.92397049172223
119	33	-0.7454462960955937
119	34	0.30771906406718585
119	35	-0.024914908248717893
119	36	2.6382725052935605
119	37	2.307285988731948
119	38	2.563514333858002
119	39	4.963305335037997
119	40	8.112601015268012
119	41	5.79163228460779
119	42	1.9088318009958982
119	43	-1.2116419102827898
119	44	-3.2207209040426896
119	45	-1.2443659010873596
119	46	-7.720351682983722
119	47	-10.066640592667104
119	48	-10.623919968872466
119	49	-8.571170304219455
119	50	-7.510020211699416
119	51	-9.595057237020317
119	52	-8.568270729036545
119	53	-12.386513117745896
119	54	-12.739161003900653
119	55	-13.11016904619531
119	56	-10.315950965398324
120	1	0.3909771645440827
120	2	1.240799718382732
120	3	0.7375005955128131
120	4	3.940054607104514
120	5	3.388288869969635
120	6	0.5137272231039844
120	7	0.5618837660576403
120	8	0.4979227451233541
120	9	0.8356187750623008
120	10	1.1542553718994455
120	11	-12.15685274086346
120	12	-3.720828427610602
120	13	-8.325406077464603
120	14	-8.983135793023216
120	15	-7.481711352901176
120	16	-8.61142924982655
120	17	-8.524002740271428
120	18	-3.8627462097941283
120	19	-3.152417242969939
120	20	-3.875928827096068
120	21	-0.45278033088392533
120	22	0.33251992815345943
120	23	-0.9670151166153858
120	24	-5.102563037549569
120	25	-0.900036588854725
120	26	0.8468084514104888
120	27	0.3041252194256856
120	28	0.5292170402545722
120	29	1.4262659664126005
120	30	1.1015219931852727
120	31	-4.485571221484673
120	32	-10.58306140081314
120	33	-2.749991750641044
120	34	-11.615008208660086
120	35	-2.6885512718850784
120	36	-3.1185456765246258
120	37	-3.05407764763169
120	38	-1.1296674843238144
120	39	0.17921442594708736
120	40	-3.3851262574592624
120	41	-0.08336771539221033
120	42	-5.232077289913192
120	43	-4.8002782739191545
120	44	-5.222993631315415
120	45	-9.596638628360086
120	46	-12.099897137529178
120	47	-11.830276956303468
120	48	-9.587556332508829
120	49	-14.755261213310366
120	50	-9.357747484426689
120	51	-8.606420873383954
120	52	-8.068270729036545
120	53	-7.720604026836805
120	54	-8.895979185718835
120	55	-7.716987228013492
120	56	-3.409132783580141
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	501.0
5	22.0
6	23.0
7	57.0
8	114.0
9	139.0
10	104.0
11	56.0
12	84.0
13	96.0
14	134.0
15	239.0
16	290.0
17	402.0
18	522.0
19	647.0
20	763.0
21	929.0
22	1174.0
23	1482.0
24	1694.0
25	1810.0
26	1913.0
27	2048.0
28	2186.0
29	2260.0
30	2553.0
31	2983.0
32	3425.0
33	4322.0
34	4926.0
35	6082.0
36	6956.0
37	6391.0
38	3787.0
39	1165.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.54560051380861	0.401412973667309	0.04174694926140013	0.01123956326268465
8	99.52954399486191	0.30346820809248554	0.11078998073217727	0.05619781631342324
9	98.1529961614442	1.0278977884136646	0.35976422594478263	0.45934182419735636
10	51.255619781631346	32.26075786769428	7.07771355170199	9.405908798972384
11	32.85216525634644	26.917580564877408	18.412305913710885	21.817948265065272
12	33.94187540141297	14.34649967886962	23.118175979447656	28.59344894026975
13	26.2106615285806	14.189145793192036	25.828516377649326	33.77167630057804
14	23.762105905594012	16.581276199347926	26.39127571752084	33.26534217753722
15	24.042080880223917	18.420036675996528	25.441559695010135	32.096322748769424
16	28.32704200465826	20.732471287446792	23.567584932937113	27.372901774957835
17	28.02743158165232	25.197545933444687	20.867596042657073	25.907426442245924
18	29.17362538540596	24.23882322713258	22.475590955806783	24.111960431654676
19	28.765979315218086	27.820068092760327	17.893621121603392	25.520331470418196
20	29.435574544636832	26.31790292010665	20.188570143596003	24.057952391660507
21	29.425556858147715	24.15969422987361	22.93597135011001	23.478777561868664
22	27.469933685511972	24.391849580115288	23.083222273960725	25.05499446041202
23	26.374438021836866	25.833333333333336	24.393063583815028	23.399165061014774
24	26.058124598587025	25.666345536287732	22.130700064226076	26.144829800899167
25	26.7778866070425	26.02321809919877	22.07325101559113	25.125644278167602
26	22.96838548789317	26.577105623039177	25.257823183975546	25.196685705092108
27	22.634326560115653	21.967713436671755	27.289374347441974	28.108585655770618
28	20.74333778285273	25.056691165827694	28.424387654996057	25.77558339632352
29	23.109790378282867	24.85583487270099	28.21781383021444	23.816560918801702
30	22.787432940345013	24.438626361270842	31.85132834334543	20.922612355038712
31	23.31911468812877	24.548893360160967	31.991951710261567	20.14004024144869
32	25.865253352605798	20.610294708102465	30.938729623383924	22.585722315907812
33	25.79098907815792	22.049574546799853	32.83951808779295	19.319918287249273
34	22.979270448336813	22.863570625100436	37.41443033906476	16.74272858749799
35	22.075314076406517	25.60967773029592	36.024804806734565	16.290203386562993
36	21.110789422891337	25.844431576915657	33.881811748053785	19.162967252139225
37	21.13592092341871	26.30036865914323	33.897322794082136	18.666387623355927
38	23.66182689370186	26.10743974402264	33.889666039586444	16.34106732268905
39	20.66347502297016	21.267952995792832	30.06754033883005	28.00103164240695
40	13.921882322522261	15.930576428952344	24.792747369952004	45.35479387857339
41	15.05468686705015	17.661832617678037	27.748521429150124	39.53495908612169
42	17.513560495098908	25.678829532102558	37.024577894381046	19.783032078417488
43	11.668654695509204	14.661981366259388	28.397111447822304	45.2722524904091
44	13.103715545221139	15.335303331234154	26.79681570831113	44.76416541523358
45	19.584231004212356	23.36409530853082	36.078330492941895	20.973343194314932
46	19.091318183279228	24.243836585130662	37.07531098325351	19.5895342483366
47	17.524580682475417	22.137073452862925	34.666795193110985	25.67155067155067
48	19.019084950520497	25.531743991774835	36.93130702994474	18.517864027759927
49	14.335213537469782	16.780016116035455	26.275584206285252	42.60918614020951
50	18.352566269132346	24.820948610239324	37.60803437786684	19.218450742761494
51	20.28298846129053	26.165151808160896	35.45735834461419	18.09450138593438
52	18.080252784987668	26.7084750681133	34.87401054345548	20.33726160344355
53	18.924703496886114	26.605622696770247	36.1645290548913	18.305144751452342
54	17.003312855810364	23.030780611752597	33.46788459682866	26.498021935608374
55	19.56234915526951	24.656476267095737	36.96701528559936	18.8141592920354
56	18.012712205326253	24.466972403250463	34.76224957760078	22.758065813822512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	3.0
9	4.0
10	31.5
11	59.0
12	124.0
13	260.0
14	331.0
15	404.0
16	477.0
17	507.0
18	537.0
19	586.5
20	636.0
21	700.0
22	862.5
23	961.0
24	1198.5
25	1436.0
26	1699.0
27	1962.0
28	2098.5
29	2271.0
30	2307.0
31	2255.0
32	2203.0
33	2008.0
34	1813.0
35	1615.5
36	1418.0
37	1269.5
38	1117.5
39	1114.0
40	1138.0
41	1162.0
42	1173.0
43	1184.0
44	1197.5
45	1211.0
46	1319.0
47	1447.0
48	1467.0
49	1592.5
50	1718.0
51	1869.0
52	2020.0
53	2192.0
54	2559.5
55	2755.0
56	3059.5
57	3364.0
58	3763.0
59	4162.0
60	4240.5
61	4319.0
62	4114.0
63	3624.0
64	3339.0
65	2963.5
66	2588.0
67	2269.5
68	1951.0
69	1648.5
70	1346.0
71	1151.5
72	810.5
73	664.0
74	540.0
75	416.0
76	308.0
77	200.0
78	158.5
79	81.5
80	46.0
81	33.0
82	20.0
83	12.0
84	4.0
85	2.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.027296082209377005
10	0.0
11	0.0016056518946692357
12	0.0
13	0.0
14	0.027296082209377005
15	0.18304431599229287
16	0.04014129736673089
17	0.02569043031470777
18	0.012845215157353885
19	0.01926782273603083
20	0.035324341682723186
21	0.017662170841361593
22	0.0016056518946692357
23	0.0
24	0.0
25	0.0016056518946692357
26	0.2007064868336545
27	0.04014129736673089
28	0.16217084136159282
29	0.04014129736673089
30	0.035324341682723186
31	0.2488760436737315
32	0.024084778420038536
33	0.17822736030828518
34	0.08028259473346178
35	0.05459216441875401
36	0.17341040462427745
37	0.26172125883108543
38	0.13969171483622353
39	0.3901734104046243
40	0.640655105973025
41	0.9071933204881182
42	0.24245343609505457
43	0.38856775850995506
44	0.5635838150289018
45	0.13166345536287732
46	0.09312780989081568
47	0.05780346820809249
48	0.05138086062941554
49	0.3692999357739242
50	0.23603082851637763
51	0.36608863198458574
52	0.40301862556197815
53	0.2231856133590238
54	0.1573538856775851
55	0.20873474630700067
56	0.2167630057803468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	62280.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.878612716763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.49079618540696	81.22350674373796
2	4.320987654320987	7.508028259473345
3	1.0349671028313743	2.697495183044316
4	0.38626450802099505	1.342324983943481
5	0.23656390921859985	1.0276172125883107
6	0.14230797663931397	0.7418111753371869
7	0.07207806609004214	0.43834296724470134
8	0.05174835514156871	0.3596660244059088
9	0.03696311081540622	0.2890173410404624
>10	0.2106897316478155	3.0491329479768785
>50	0.011088933244621867	0.6567116249197174
>100	0.005544466622310933	0.6663455362877329
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCAATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	141	0.22639691714836221	No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	140	0.22479126525369297	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	134	0.2151573538856776	No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	92	0.1477199743095697	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	86	0.13808606294155426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.009633911368015413	0.0	0.0	0.0	0.0
11	0.019267822736030827	0.0	0.0	0.0	0.0
12	0.024084778420038536	0.0	0.0	0.0	0.0
13	0.027296082209377008	0.0	0.0	0.0	0.0
14	0.03211303789338472	0.0	0.0	0.0	0.0
15	0.03371868978805395	0.0	0.0	0.0	0.0
16	0.03371868978805395	0.0	0.0	0.0	0.0
17	0.035324341682723186	0.0	0.0	0.0	0.0
18	0.04977520873474631	0.0	0.0	0.0	0.0
19	0.057803468208092484	0.0	0.0	0.0	0.0
20	0.06101477199743096	0.0	0.0	0.0	0.0
21	0.07546563904945408	0.0	0.0	0.0	0.0
22	0.08028259473346179	0.0	0.0	0.0	0.0
23	0.0850995504174695	0.0	0.0	0.0	0.0
24	0.08831085420680797	0.0	0.0	0.0	0.0
25	0.09794476557482337	0.0	0.0	0.0	0.0
26	0.10436737315350032	0.0	0.0	0.0	0.0
27	0.10918432883750803	0.0	0.0	0.0	0.0
28	0.11881824020552344	0.0	0.0	0.0	0.0
29	0.12042389210019268	0.0	0.0	0.0	0.0
30	0.12202954399486192	0.0	0.0	0.0	0.0
31	0.1252408477842004	0.0	0.0	0.0	0.0
32	0.1252408477842004	0.0	0.0	0.0	0.0
33	0.1252408477842004	0.0	0.0	0.0	0.0
34	0.1252408477842004	0.0	0.0	0.0	0.0
35	0.1252408477842004	0.0	0.0	0.0	0.0
36	0.1252408477842004	0.0	0.0	0.0	0.0
37	0.1252408477842004	0.0	0.0	0.0	0.0
38	0.1252408477842004	0.0	0.0	0.0	0.0
39	0.1252408477842004	0.0	0.0	0.0	0.0
40	0.1252408477842004	0.0	0.0	0.0	0.0
41	0.1252408477842004	0.0	0.0	0.0	0.0
42	0.12845215157353887	0.0	0.0	0.0	0.0
43	0.13005780346820808	0.0	0.0	0.0	0.0
44	0.1348747591522158	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGACC	20	3.9242854E-4	50.57667	38
ACGCCCA	30	9.531377E-7	50.57667	39
TACGCCC	30	9.531377E-7	50.57667	38
AAACCAC	15	0.008047208	50.535454	37
CAACCTG	25	1.9475601E-5	50.4943	41
ATATGCT	15	0.0082304245	50.248783	34
TAGAGCC	45	1.2551027E-10	50.248783	34
TAAAGGC	15	0.0082304245	50.248783	34
CTCTGAG	15	0.008363181	50.04601	47
TATATTT	15	0.008363181	50.04601	46
AACGATT	15	0.008363181	50.04601	47
CTTATAT	15	0.008363181	50.04601	44
ACGATTA	15	0.008363181	50.04601	48
TCTCCAG	15	0.008363181	50.04601	46
TCACAAC	15	0.008363181	50.04601	46
CAACGAT	15	0.008363181	50.04601	46
CGATTAA	15	0.008363181	50.04601	49
ACGGGTA	40	2.6102498E-9	50.005646	33
GTGTCTA	15	0.008443596	49.925125	23
AACCGTG	15	0.008443596	49.925125	25
>>END_MODULE