FastQCFastQC Report
Fri 17 Jun 2016
SRR1526356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7706
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC140.18167661562418894No Hit
TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA130.16869971450817545No Hit
TTCCGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC120.15572281339216196No Hit
TTCCGCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCCCGCAGCATTTGATATG100.12976901116013498No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC100.12976901116013498No Hit
TTCCGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC90.11679211004412147No Hit
TTCCGCGGGGTGTAGTACTGTCTGGACCAGTCAGCTGTCGGGTTAGTGCTAGTGGT90.11679211004412147No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC80.10381520892810796No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT80.10381520892810796No Hit
TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA80.10381520892810796No Hit
TTCCGCGGGGATCCAGTGACAGATACCATTAGATTCAAGCCATAGAATATGAAGGA80.10381520892810796No Hit
TTCCGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT80.10381520892810796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTC308.8183333E-750.27631850
GGGTCCG251.8357068E-550.27631836
GTGCAGT150.00793951650.27631831
GAGTGCA150.00793951650.27631829
GGTCCGC203.8197593E-450.27631837
CCTCGGC150.00793951650.27631837
GTCCGCA203.8197593E-450.27631838
GACGGGT251.8357068E-550.27631833
AGGAATT203.8197593E-450.27631826
CCGCACA203.8197593E-450.27631840
CGGGTCC251.8357068E-550.27631835
CACCCGA203.8197593E-450.27631841
AGTGCAG203.8197593E-450.27631830
ACGGGTC251.8357068E-550.27631834
AAAGGAA203.8197593E-450.27631824
TCTGAGT251.8357068E-550.27631849
GAATTGA308.8183333E-750.27631828
ATTGACG308.8183333E-750.27631830
TCCGCAC203.8197593E-450.27631839
AAGGAAT203.8197593E-450.27631825
Produced by FastQC (version 0.11.5)