FastQCFastQC Report
Fri 17 Jun 2016
SRR1526355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91542
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1640.17915273863363265No Hit
TATTGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1570.1715059753992703No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1340.14638089620065106No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1220.13327215922745844No Hit
TATTGTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1130.12344060649756396No Hit
TATTGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT1090.11907102750649974No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC990.10814708002883922No Hit
TATTGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT960.10486989578554107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCT150.00789768750.79497538
TGCAGGT150.00789768750.79497539
ACAACAA402.2919266E-950.7949739
ACCCACC203.8513477E-450.7949738
TTACTCC150.00814488750.4007835
CGATTAA150.00819859950.31710449
TAAAACA150.00819859950.31710449
ACGATTA150.00819859950.31710448
CGATTCA204.0354414E-450.31709749
TATGAAG204.0354414E-450.31709748
ACGATTC204.0354414E-450.31709748
ACCGGAA150.00821656150.28927242
CAACGAT301.000466E-650.26147546
ATATGAA204.057551E-450.2614747
AACGATT252.009161E-550.2614747
GCCTACC150.00825257350.233744
AGAATAT204.068642E-450.233744
GCCACGA150.00827062450.20596345
GAATATG204.079757E-450.2059645
TGATACC150.0083249550.12293234