FastQCFastQC Report
Fri 17 Jun 2016
SRR1526354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46767
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1240.265144225629183No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1120.23948510701990722No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG970.20741120875831248No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC960.20527294887420616No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC950.20313468899009987No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG870.18602860991724934No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG810.17319905061261145No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG810.17319905061261145No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG810.17319905061261145No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG760.16250775119207989No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG720.15395471165565464No Hit
GCAGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG700.149678191887442No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTC580.12401907327816623No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG530.11332777385763466No Hit
GCAGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG520.11118951397352833No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG500.1069129942053157No Hit
GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG470.10049821455299678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTC150.00796848150.6409138
ACTTCAG150.00796848150.6409140
CTGTCGA150.00796848150.6409140
CAAACCA309.396008E-750.6409139
CCGGTCA150.00796848150.6409140
TGTCGAC150.00800286650.5858741
AGCCCCT150.00810668350.42144836
TACAAAC150.00810668350.42144837
AGAAACT150.00810668350.42144836
CTTATCT150.00821149950.258144
CGACTCC150.00821149950.258144
CCTGGCG150.00821149950.258144
TTCACCC204.0815113E-450.14978847
GTTCACC204.0815113E-450.14978846
TCAGTTC204.0815113E-450.14978843
CCCGCTC204.0815113E-450.14978847
GCTCTGA204.0815113E-450.14978846
TCTCACG355.023503E-850.14978848
CCAAAAC252.020302E-550.14978443
CCGCTCT451.2732926E-1050.14978448