##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	46767
Sequences flagged as poor quality	0
Sequence length	56
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.4573524066115	39.0	38.0	39.0	33.0	40.0
2	36.66617486689332	39.0	37.0	39.0	31.0	40.0
3	36.10280753522783	38.0	36.0	39.0	30.0	40.0
4	36.805867385121985	38.0	36.0	39.0	33.0	40.0
5	36.85280218957812	38.0	37.0	39.0	33.0	40.0
6	34.74184788419184	38.0	35.0	39.0	22.0	40.0
7	36.36773793486861	38.0	36.0	39.0	31.0	40.0
8	36.23948510701991	38.0	36.0	39.0	31.0	40.0
9	35.21393290140484	38.0	35.0	39.0	27.0	40.0
10	35.12765411508115	38.0	35.0	39.0	28.0	40.0
11	33.66516988474779	36.0	31.0	39.0	24.0	40.0
12	32.182671541899204	36.0	29.0	39.0	17.0	40.0
13	31.81921012679881	35.0	29.0	39.0	16.0	40.0
14	31.43344666110719	36.0	27.0	39.0	13.0	40.0
15	32.09979258879124	36.0	29.0	38.0	16.0	40.0
16	32.47287617337011	36.0	30.0	38.0	18.0	40.0
17	32.506318557957535	36.0	30.0	38.0	18.0	40.0
18	32.62670258943272	36.0	30.0	38.0	19.0	40.0
19	33.39459447901298	36.0	31.0	38.0	24.0	40.0
20	33.54912652083734	36.0	31.0	38.0	24.0	40.0
21	34.88355036671157	38.0	34.0	39.0	27.0	40.0
22	34.516197318622105	38.0	33.0	39.0	25.0	40.0
23	34.64365899031368	38.0	33.0	39.0	26.0	40.0
24	34.320268565441445	38.0	33.0	39.0	24.0	40.0
25	34.5623837321188	38.0	33.0	39.0	26.0	40.0
26	34.862060854876304	38.0	35.0	39.0	27.0	40.0
27	34.82380738554964	38.0	35.0	39.0	27.0	40.0
28	34.669125665533386	38.0	34.0	39.0	27.0	40.0
29	34.59918318472427	38.0	34.0	39.0	27.0	40.0
30	34.35734599183185	38.0	33.0	39.0	25.0	40.0
31	34.27066093613018	38.0	33.0	39.0	25.0	40.0
32	33.150939765219064	38.0	33.0	39.0	18.0	40.0
33	34.4299612974961	38.0	34.0	39.0	27.0	40.0
34	34.15808155323198	38.0	33.0	39.0	25.0	40.0
35	34.07586546068809	38.0	33.0	39.0	25.0	40.0
36	31.96967947484337	35.0	31.0	38.0	21.0	39.0
37	31.821690508264375	35.0	31.0	38.0	19.0	39.0
38	30.809010627151626	34.0	30.0	38.0	12.0	39.0
39	28.951226292043536	33.0	26.0	38.0	5.0	39.0
40	24.78200440481536	30.0	11.0	36.0	4.0	39.0
41	21.27536510787521	24.0	8.0	33.0	4.0	36.0
42	25.09087604507452	30.0	19.0	33.0	4.0	36.0
43	21.416875147005367	26.0	8.0	33.0	4.0	36.0
44	22.432869330938484	27.0	9.0	33.0	4.0	36.0
45	24.835653345307588	30.0	19.0	33.0	4.0	36.0
46	25.09395513930763	30.0	15.0	35.0	4.0	38.0
47	24.264053713088288	30.0	9.0	35.0	4.0	38.0
48	24.397267303868112	30.0	10.0	35.0	4.0	38.0
49	21.81318023392563	26.0	5.0	35.0	4.0	38.0
50	24.195843222785296	29.0	4.0	35.0	4.0	38.0
51	25.697393461201273	32.0	4.0	38.0	4.0	39.0
52	25.497765518421108	32.0	4.0	38.0	4.0	39.0
53	25.183847584835462	32.0	4.0	38.0	4.0	39.0
54	24.281074261765774	31.0	4.0	37.0	4.0	39.0
55	24.427480916030536	31.0	4.0	37.0	4.0	39.0
56	23.766330959864863	31.0	4.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.4850720485278188
1	2	1.6595172238917257
1	3	2.2477490638983113
1	4	1.0043887101146964
1	5	1.0528550563200127
1	6	3.5798991919661702
1	7	1.9032431927787599
1	8	2.012178708867012
1	9	1.7920689522064706
1	10	1.0625144549352896
1	11	-5.481044914650791
1	12	-9.620181396692399
1	13	-21.635157852605087
1	14	-19.293884380626896
1	15	-13.336634276435145
1	16	-20.96713888825397
1	17	-21.69944710613628
1	18	-21.541121534291975
1	19	-8.100079664840809
1	20	-12.445229384492304
1	21	-10.76364624184935
1	22	-5.371308459704192
1	23	-6.547568264008056
1	24	-6.22354530633509
1	25	-5.607424701314592
1	26	1.9470172550346803
1	27	2.064586238823658
1	28	1.7137800204993994
1	29	1.600942199822832
1	30	1.8610465308725423
1	31	0.37903064967216693
1	32	-12.41322027453053
1	33	-0.1690486926324155
1	34	-0.03166269650454012
1	35	0.4797600451787787
1	36	2.512084323243908
1	37	2.301863818353464
1	38	3.7163041545439484
1	39	5.585108805023925
1	40	8.312683092175043
1	41	-7.142470812911709
1	42	-11.259779538688854
1	43	-13.62323665407962
1	44	-15.971025608903759
1	45	-17.05055677662478
1	46	-18.15132797582729
1	47	-17.561656086900264
1	48	-17.477698921269514
1	49	-14.671909829717077
1	50	-17.105076271869756
1	51	-18.460384541746503
1	52	-18.128189895613787
1	53	-17.90210026153778
1	54	-17.62152318435241
1	55	-17.130957532106944
1	56	-16.820407776459042
2	1	0.37754516680738703
2	2	1.1863989443218372
2	3	1.9527221821778795
2	4	1.3363779574265209
2	5	1.4466722606210922
2	6	2.9985819876650908
2	7	1.9328130852518726
2	8	2.0558615045659323
2	9	2.333735618873135
2	10	1.6801219818170097
2	11	-4.546905129704555
2	12	-10.97569214938057
2	13	-13.826690110669603
2	14	-15.84227147740109
2	15	-8.473059007617941
2	16	-3.7850152323399904
2	17	-2.1772696867814396
2	18	-9.586820459023158
2	19	-1.8621764390343571
2	20	-4.0890465887933765
2	21	2.524660209763553
2	22	2.8558420779302196
2	23	2.1762220585725913
2	24	2.3147611452778136
2	25	3.074026911588632
2	26	1.6412376851422081
2	27	2.136494840974194
2	28	1.8656617409295109
2	29	2.3240604793927204
2	30	2.406073412592974
2	31	-1.510082253553641
2	32	-8.460935328293974
2	33	-1.828994929191559
2	34	-1.6230605459669079
2	35	-0.9483313526706851
2	36	3.4240466888353076
2	37	3.8603315602889445
2	38	5.167917057769756
2	39	7.781345364163709
2	40	8.442387393250314
2	41	3.590056068808721
2	42	1.2623978806659863
2	43	-3.693129127197899
2	44	-10.455568619656445
2	45	-2.544508389528005
2	46	-12.662080663999332
2	47	-12.267301248190586
2	48	-10.509284942774892
2	49	-11.67795821681385
2	50	-10.781823583697713
2	51	-10.836056584757255
2	52	-11.363404949377228
2	53	-12.295917465838853
2	54	-15.190743614459935
2	55	-11.479075811676838
2	56	-10.93734326033001
3	1	1.2450720485278168
3	2	2.306183890558394
3	3	3.0535823972316436
3	4	1.7393887101146959
3	5	1.411188389653347
3	6	4.294065858632834
3	7	2.646576526112092
3	8	2.9380120422003415
3	9	3.573735618873137
3	10	2.590014454935293
3	11	0.6472884186825425
3	12	0.6939852699742701
3	13	-6.0776578526050855
3	14	-8.620551047293564
3	15	-4.387467609768478
3	16	1.193694445079366
3	17	1.006386227197055
3	18	1.0022117990413584
3	19	-0.2817463315074775
3	20	-0.04689605115896711
3	21	2.6188537581506495
3	22	3.8136915402958067
3	23	2.5815984026586136
3	24	3.1822880269982434
3	25	3.114241965352072
3	26	2.1061839217013514
3	27	1.7420862388236529
3	28	2.172113353832735
3	29	2.237608866489495
3	30	1.938546530872543
3	31	2.528197316338833
3	32	1.3792797254694698
3	33	2.489284640700916
3	34	1.615003970162121
3	35	2.0039267118454447
3	36	4.207917656577241
3	37	4.422697151686794
3	38	5.476304154543946
3	39	7.621775471690594
3	40	10.051849758841712
3	41	10.52919585375496
3	42	8.946053794644481
3	43	4.876763345920381
3	44	5.429807724429576
3	45	8.915276556708552
3	46	0.862005357506046
3	47	-4.326656086900265
3	48	0.6881344120638175
3	49	-8.11774316305041
3	50	0.3274237281302419
3	51	-1.6212178750798358
3	52	-1.5365232289471216
3	53	-2.1204335948711126
3	54	-4.775689851019074
3	55	-1.0059575321069438
3	56	2.044592223540956
4	1	1.622326950488599
4	2	2.2810858513427092
4	3	3.3327980835061553
4	4	2.3637024356048926
4	5	2.1876589778886455
4	6	4.51053644686813
4	7	3.0081451535630706
4	8	3.25016890494544
4	9	4.521970912990781
4	10	3.542171317680392
4	11	4.197876653976657
4	12	2.9222205640919157
4	13	4.602342147394911
4	14	3.629645031137809
4	15	5.359199056898184
4	16	6.24781209213819
4	17	5.5836411291578365
4	18	5.301035328453121
4	19	3.883743864570956
4	20	3.9287902233508376
4	21	4.134147875797709
4	22	3.743887618727179
4	23	2.9737552654037103
4	24	3.9360135171943185
4	25	3.473457651626582
4	26	2.7516741177797783
4	27	3.039341140784437
4	28	1.2662310008915583
4	29	3.4940794547247904
4	30	2.867173981852936
4	31	2.859177708495693
4	32	3.985554235273387
4	33	2.4932062093283633
4	34	2.4644157348680054
4	35	2.295691417727795
4	36	3.1961529506948914
4	37	4.607010877176993
4	38	4.622186507485125
4	39	6.744912726592556
4	40	9.664398778449552
4	41	3.6923331086569178
4	42	7.840955755428794
4	43	6.75127314984195
4	44	10.189023410704085
4	45	7.744296164551688
4	46	7.691809279074672
4	47	7.948638030746796
4	48	7.4622520591226404
4	49	5.83911958204763
4	50	8.046639414404755
4	51	5.24231153668487
4	52	5.706614025954838
4	53	5.063095816893593
4	54	-0.4776506353328003
4	55	5.962669918873448
4	56	4.745768694129193
5	1	1.7350720485278188
5	2	2.5622950016695043
5	3	3.21302684167609
5	4	2.3099442656702465
5	5	2.219521722986684
5	6	4.586843636410613
5	7	2.40324319277876
5	8	2.505234264422562
5	9	4.000402285539799
5	10	2.722236677157511
5	11	4.963399529793655
5	12	7.247874158863155
5	13	6.0523421473949135
5	14	6.567226730484219
5	15	6.517532390231523
5	16	6.817583333968251
5	17	6.224164004974835
5	18	5.472767354596915
5	19	5.011031446270305
5	20	4.846437282174364
5	21	3.0210759803728706
5	22	3.1286915402958115
5	23	2.806598402658608
5	24	2.936176915887131
5	25	3.2120197431298507
5	26	1.7456283661457945
5	27	2.0159751277125437
5	28	2.0748911316105136
5	29	1.8231644220450534
5	30	1.9235465308725423
5	31	2.0943084274499455
5	32	3.7326130588028015
5	33	1.4559513073675845
5	34	2.4822261923843456
5	35	2.028371156289886
5	36	3.630139878799465
5	37	3.739363818353464
5	38	4.674637487877284
5	39	6.223997693912818
5	40	6.639071981063935
5	41	0.5936402981994036
5	42	9.233276016866704
5	43	11.654541123698156
5	44	11.799807724429577
5	45	9.275832112264105
5	46	9.695894246394936
5	47	9.785566135321957
5	48	10.48757885650826
5	49	10.265590170282923
5	50	9.915757061463577
5	51	8.282671013809054
5	52	8.316254548830656
5	53	8.722899738462221
5	54	7.690976815647591
5	55	8.42459802344861
5	56	7.249036667985401
6	1	1.9427834913138895
6	2	3.2689699602101356
6	3	3.6299008051918378
6	4	2.598294182751509
6	5	2.6231286881608114
6	6	5.023618097438806
6	7	3.446775531087212
6	8	3.707763285981436
6	9	4.363586365141792
6	10	3.695288086776088
6	11	5.592960060473587
6	12	7.331000195347407
6	13	6.1754764757531255
6	14	7.818055917880567
6	15	6.962184131525049
6	16	7.225336236124143
6	17	6.948873789386109
6	18	6.325793888593598
6	19	5.323427797845756
6	20	5.159870117995261
6	21	3.298853758150649
6	22	3.9054328338281508
6	23	3.2313496464397033
6	24	4.6859696190380475
6	25	3.7078738061480934
6	26	2.6600147674724965
6	27	3.0792006666843506
6	28	2.4556954433849754
6	29	3.0698476724596446
6	30	2.9602380731611007
6	31	2.807202291463213
6	32	4.590822014026681
6	33	2.4435134964223053
6	34	2.866347253744216
6	35	3.710792383487231
6	36	3.960156462547392
6	37	3.444587698950482
6	38	4.268542960514097
6	39	7.123666018954278
6	40	3.0928447837173323
6	41	2.8083998338544625
6	42	8.425854789669355
6	43	13.165320559850727
6	44	11.435379863733058
6	45	8.500749193524474
6	46	10.157129735615499
6	47	10.701403614592273
6	48	10.33231351654143
6	49	12.06036628968591
6	50	10.824339151015817
6	51	10.451617945815684
6	52	10.225665825779249
6	53	9.72911864393486
6	54	10.17853900470232
6	55	8.909465353465194
6	56	8.580711626526032
7	1	1.6728654757578667
7	2	2.5914421065208373
7	3	3.339591786903007
7	4	2.3699990387531926
7	5	2.025272896695604
7	6	4.638291210745514
7	7	3.369205634093312
7	8	3.5688101642660683
7	9	4.573172238591447
7	10	3.5199675065784817
7	11	5.163908136992397
7	12	6.635393720678493
7	13	7.774173133310409
7	14	7.192031112330849
7	15	6.953565254081283
7	16	7.004398670431478
7	17	6.324320499497521
7	18	6.281648418759669
7	19	5.433371039384543
7	20	4.930944324427884
7	21	3.7777269975872656
7	22	3.3851469393568436
7	23	3.5571852571187037
7	24	4.17346173591843
7	25	3.8802513550234394
7	26	2.9379210109032243
7	27	3.2955134688706025
7	28	2.1439443397482307
7	29	3.024651120010624
7	30	2.87131648392419
7	31	2.673925015869351
7	32	4.105852495422521
7	33	2.2141672698088968
7	34	2.6138772095987406
7	35	2.759983049873611
7	36	-0.12729361102838865
7	37	0.4893638183534641
7	38	1.2374309151073284
7	39	3.5909773496248647
7	40	-2.9992300533648617
7	41	-9.515123395071333
7	42	5.038635954268894
7	43	9.153758651084697
7	44	8.601450916917837
7	45	5.360534772670995
7	46	8.120972493656279
7	47	8.496442504649032
7	48	8.70334567966945
7	49	11.409956367466023
7	50	8.554841568505829
7	51	9.553993392525797
7	52	9.10479132504349
7	53	10.400129785410577
7	54	10.146375876680455
7	55	9.355544815310896
7	56	9.663747153118422
8	1	1.8640674823177719
8	2	2.6846313791428713
8	3	3.692669155222511
8	4	2.7857814041786213
8	5	2.435845923899926
8	6	5.20803846137256
8	7	3.0664852019111777
8	8	3.5940851015610704
8	9	4.662502742160804
8	10	3.7682792951179422
8	11	5.398521295394872
8	12	7.624396228878382
8	13	7.583164065203132
8	14	7.60337589334571
8	15	7.4907059062132575
8	16	7.909858828641006
8	17	7.250039195233587
8	18	6.836732346986562
8	19	5.887934033789325
8	20	4.764245501352448
8	21	3.65501814171229
8	22	4.262823960387131
8	23	4.07657557160838
8	24	3.9245711320210717
8	25	3.3566163945758163
8	26	3.1887409993269173
8	27	3.1550542753533364
8	28	2.021428422325883
8	29	2.887471880188123
8	30	2.6866743847538217
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115	39	8.339870709785831
115	40	9.215659282651238
115	41	10.973005377564483
115	42	2.338434747025431
115	43	6.238668107825141
115	44	3.854569629191481
115	45	3.661943223375218
115	46	-0.9989470234463376
115	47	-2.2866560869002654
115	48	-2.4318655879361835
115	49	-7.670124115431362
115	50	0.5978999186064335
115	51	0.7616392677773085
115	52	0.7587148662909726
115	53	-0.33186216629968257
115	54	1.1290720537428314
115	55	1.6988043726549584
115	56	3.3188779378266737
116	1	0.8660244294801984
116	2	1.8261838905583971
116	3	2.036439540088786
116	4	1.4984363291623097
116	5	1.034997913462874
116	6	2.2673991919661702
116	7	2.415147954683519
116	8	2.7056310898193914
116	9	2.333735618873135
116	10	1.5357287406495743
116	11	-1.6060449146507914
116	12	-2.575538539549541
116	13	-7.888134043081276
116	14	-10.317693904436421
116	15	-5.655086657387528
116	16	-8.85106745968254
116	17	-10.363137582326754
116	18	-3.3863596295300695
116	19	-4.379841569602714
116	20	-3.8916579559208735
116	21	0.2988537581506492
116	22	0.5394058260100962
116	23	0.32445554551575384
116	24	-0.006283401573185188
116	25	-0.47051993940983294
116	26	1.6166601121775415
116	27	1.049705286442702
116	28	1.26735144907083
116	29	1.755704104584737
116	30	2.084260816586827
116	31	-0.05846935032783307
116	32	-12.719767893578151
116	33	0.003570354986628388
116	34	-0.3411865060283503
116	35	0.2982124261311583
116	36	1.5745843232439078
116	37	1.727459056448705
116	38	2.906780345020138
116	39	4.977965947881071
116	40	7.6728021397940935
116	41	5.506338710897815
116	42	3.0527204613111465
116	43	-8.456569987412953
116	44	-3.3073351327132805
116	45	-0.26186630043430625
116	46	-5.5798994043987165
116	47	-10.996179896424074
116	48	-8.784246540317135
116	49	-6.0082193535266
116	50	-5.054481033774518
116	51	-6.314551208413167
116	52	-5.38414227656617
116	53	-7.265195499633016
116	54	-9.094737470066693
116	55	-7.029767055916469
116	56	-4.957312538363805
117	1	0.27173871519448056
117	2	1.2928505572250586
117	3	1.6269157305649742
117	4	1.0460553767813607
117	5	1.0511883896533476
117	6	3.014065858632833
117	7	1.2199098594454227
117	8	1.5113453755336792
117	9	1.2137356188731374
117	10	0.9233477882686287
117	11	-9.512711581317458
117	12	-8.82601473002573
117	13	-7.917657852605085
117	14	-8.513884380626894
117	15	-10.920800943101813
117	16	-13.406305554920635
117	17	-8.966947106136278
117	18	-4.8377882009586415
117	19	-4.135079664840809
117	20	-1.713562717825635
117	21	0.5388537581506512
117	22	-0.1996417930375216
117	23	-0.4450682640080572
117	24	-2.1910453063350914
117	25	0.007575298685409848
117	26	1.092850588368016
117	27	0.20875290549032144
117	28	-0.3878866461672672
117	29	0.6776088664894928
117	30	-0.2481201357941245
117	31	-0.5784693503278362
117	32	-15.807386941197198
117	33	-1.8573820259657516
117	34	-2.7049960298378757
117	35	-2.4760732881545557
117	36	-1.0720823434227569
117	37	-0.9906361816465363
117	38	0.4363041545439472
117	39	2.4484421383572617
117	40	1.3985164255083795
117	41	0.7558625204216263
117	42	-1.3872795386888548
117	43	-8.109903320746287
117	44	-3.0368589422370924
117	45	-0.5380567766247815
117	46	-4.524661309160621
117	47	-11.126656086900264
117	48	-4.511865587936182
117	49	-7.104409829717076
117	50	-3.2992429385364233
117	51	-3.1412178750798354
117	52	-3.149856562280455
117	53	-3.24043359487111
117	54	-7.042356517685741
117	55	-4.112624198773613
117	56	-1.3154077764590433
118	1	0.596183159638926
118	2	1.5484061127806186
118	3	2.39358239723164
118	4	1.1016109323369179
118	5	1.4278550563200127
118	6	3.211843636410613
118	7	1.514354303889867
118	8	1.741345375533676
118	9	1.5837356188731349
118	10	0.416681121601961
118	11	-9.02271158131746
118	12	-11.65490361891462
118	13	-14.336546741493976
118	14	-1.3772177139602277
118	15	-16.107467609768477
118	16	-7.835194443809524
118	17	-11.359169328358501
118	18	-3.4022326454030853
118	19	-3.3084129981741413
118	20	-4.931340495603415
118	21	1.187742647039535
118	22	1.9203582069624758
118	23	1.431598402658608
118	24	-2.355489750779533
118	25	1.7120197431298507
118	26	2.1761839217013517
118	27	1.8354195721569866
118	28	1.3943355760549565
118	29	2.1842755331561605
118	30	2.076324308650321
118	31	0.3859750941166098
118	32	-9.10072027453053
118	33	0.2892846407009131
118	34	1.4961150812732384
118	35	-0.4994066214878927
118	36	3.2134732121328007
118	37	3.4060304850201284
118	38	4.632970821210613
118	39	6.251775471690596
118	40	8.819627536619493
118	41	6.510306964866068
118	42	1.3860537946444786
118	43	2.26565223480927
118	44	0.49425216887402
118	45	2.0952765567085514
118	46	-1.2346613091606216
118	47	-5.103322753566932
118	48	-3.7207544768250713
118	49	-6.012187607494855
118	50	-1.487020716314202
118	51	-1.2589956528576138
118	52	-1.0309676733915651
118	53	-4.054878039315554
118	54	-8.003467628796852
118	55	-4.978179754329169
118	56	-0.9454077764590423
119	1	0.3452871022912589
119	2	1.621882815289581
119	3	2.097883472500456
119	4	0.1750876348458803
119	5	1.1079625832017328
119	6	3.095356181213482
119	7	1.3682969562196163
119	8	1.4107002142433558
119	9	0.9466388446795833
119	10	0.18012198181700967
119	11	-11.56303416196262
119	12	-10.201498600993471
119	13	-2.2621739816373463
119	14	-4.8906585741752835
119	15	-9.344026749553425
119	16	-15.317273296856118
119	17	-16.370818073878215
119	18	-6.506175297732835
119	19	-4.426692568066617
119	20	-5.863240137180476
119	21	-3.1527591450751586
119	22	-2.466738567231072
119	23	-2.2592618123951524
119	24	-8.943303370851218
119	25	-3.232424701314592
119	26	-0.4232784438900481
119	27	-0.05705354612258162
119	28	-0.27949954939307275
119	29	0.09825402777981651
119	30	0.018976638399429646
119	31	-4.026211285811705
119	32	-11.573838554100423
119	33	-2.441898154998011
119	34	-2.5746734491927157
119	35	-2.754782965573913
119	36	0.9240466888353076
119	37	1.5216218828695922
119	38	2.2001751222858843
119	39	4.845861493195969
119	40	6.442387393250314
119	41	5.928765746228077
119	42	2.1494946548595344
119	43	0.5649353889311328
119	44	-1.1813750712693505
119	45	-0.673540647592521
119	46	-6.54917743819288
119	47	-9.202785119158328
119	48	-11.025413975032956
119	49	-9.82311950713643
119	50	-8.297952615955777
119	51	-10.594121100886287
119	52	-10.185985594538518
119	53	-11.747530369064659
119	54	-14.093969420911547
119	55	-12.188753231031676
119	56	-9.517988421620332
120	1	0.3576210681356571
120	2	1.6987329101662354
120	3	2.4327980835061496
120	4	1.3107612591343027
120	5	1.2170707425945224
120	6	3.6105364468681316
120	7	1.6140275065042502
120	8	1.7854630225925021
120	9	1.3631473835790189
120	10	0.8480536706215673
120	11	-14.77271158131746
120	12	-5.001308847672789
120	13	-8.3447166761345
120	14	-8.470354968862189
120	15	-4.852565648984164
120	16	-11.328658496097106
120	17	-10.357535341430395
120	18	-3.4577882009586425
120	19	-3.122138488370222
120	20	-3.9770921295903428
120	21	0.03414787579770717
120	22	0.9791817363742439
120	23	-0.04389179341982441
120	24	-6.234574718099797
120	25	0.26757529868540786
120	26	1.79873294130919
120	27	2.0511058466667933
120	28	2.348583942068032
120	29	2.5470206311953802
120	30	2.278938687735291
120	31	-2.1172928797395976
120	32	-10.826210470608963
120	33	-1.2891467318481062
120	34	-11.570878382779053
120	35	-0.386661523448673
120	36	0.819682362459595
120	37	0.10701087717699309
120	38	0.33395121336747735
120	39	4.080206844239612
120	40	2.2232223078613202
120	41	4.345274285127509
120	42	-0.27080895045356
120	43	-1.6134327325109936
120	44	-3.175682471648855
120	45	-6.208645011918897
120	46	-14.014073073866504
120	47	-13.157244322194382
120	48	-12.155394999700889
120	49	-14.337351006187664
120	50	-10.900419409124659
120	51	-10.834159051550424
120	52	-10.758091856398101
120	53	-11.47808065369464
120	54	-10.730591811803388
120	55	-11.672624198773612
120	56	-8.136584247047278
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	418.0
5	76.0
6	64.0
7	71.0
8	69.0
9	101.0
10	64.0
11	69.0
12	78.0
13	92.0
14	120.0
15	187.0
16	310.0
17	366.0
18	469.0
19	557.0
20	587.0
21	859.0
22	1045.0
23	1233.0
24	1249.0
25	1415.0
26	1502.0
27	1566.0
28	1712.0
29	1785.0
30	2013.0
31	2321.0
32	2808.0
33	3308.0
34	4076.0
35	4800.0
36	5073.0
37	3966.0
38	1949.0
39	389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.52316804584429	0.3763337396027113	0.07056257617550837	0.029935638377488402
8	99.4611585092052	0.3677807000662861	0.09835995466889046	0.07270083605961468
9	98.87284782376217	0.7015292482087477	0.2288525291412683	0.1967703988878195
10	58.39587743494344	29.28560737272008	5.617208715547287	6.701306476789188
11	37.01969337353262	26.520837342570612	20.289948040284816	16.169521243611946
12	41.48865653131482	13.693416297816837	19.4709945046721	25.34693266619625
13	26.920691940898493	16.55013150298287	22.438899223811664	34.09027733230697
14	26.845178262078406	14.066343007464125	26.674080886284408	32.41439784417307
15	24.856494174091846	20.206477039067856	25.177775873886223	29.759252912954075
16	29.223793125574833	15.697388402883236	28.88584689752529	26.19297157401664
17	34.4874783464146	19.76090164460318	22.032122158301075	23.71949785068115
18	34.66843444603639	21.67525607852362	20.742895023843637	22.91341445159635
19	28.087508821453778	30.241012809820152	18.45555056564231	23.215927803083765
20	28.41803559206023	23.46851471594798	20.396988364134156	27.716461327857633
21	27.12400829715801	22.735923700361397	27.596604152855893	22.543463849624704
22	26.06324972737186	21.348386682917443	28.364017362670257	24.224346227040435
23	28.605640729574272	25.481643038894948	25.695469027305577	20.217247204225202
24	29.118823101759787	25.02191716381209	23.56576218273569	22.293497551692433
25	26.63416511642825	25.755340304060553	23.55934740308337	24.051147176427822
26	26.492193690703104	18.947165528023472	30.030197245839847	24.530443535433577
27	26.914440904133606	16.078951307657764	30.515578555695743	26.491029232512886
28	21.434230530395496	25.673968437506687	29.067900044966915	23.823900987130898
29	23.574957756721492	25.630440827326588	29.863324278655917	20.931277137296004
30	23.189614611403396	17.034518157320672	40.14714059626161	19.628726635014328
31	23.36168298378286	16.532059384305576	40.264358705199335	19.841898926712226
32	32.06945959239537	14.018091999743376	33.444537114261884	20.467911293599368
33	26.053065507420175	20.253977771591323	36.7239865515986	16.968970169389898
34	22.4081484176064	15.62707294631203	40.64579633235615	21.31898230372542
35	25.23692373515884	20.738046849930473	39.11220451385175	14.912824901058938
36	24.758580819219322	23.668715071836928	36.90769329593388	14.665010813009872
37	27.040116578090178	20.540459454825992	36.23564203668781	16.183781930396023
38	22.44299325554009	22.055454448131893	40.80077079541805	14.700781500909969
39	21.839129781801798	21.52803106696132	32.47870582934627	24.154133321890622
40	13.895578874935488	13.078444864957852	25.879494237054878	47.146482023051774
41	17.43190493648774	15.544868338760809	28.458668506976643	38.56455821777481
42	16.657384002399212	19.80206503577396	38.68514630906988	24.855404652756953
43	11.693409987986957	13.521108632229279	30.433756650077225	44.35172472970654
44	13.047868683396354	12.568752148504641	27.814541079408734	46.56883808869027
45	19.373969640524972	22.81242640289464	38.309032907273	19.504571049307383
46	24.743702245146928	18.99492755174111	37.887121974188304	18.374248228923655
47	15.638502673796792	17.424598930481284	41.79465240641711	25.14224598930481
48	18.628185105153932	17.3659100147622	45.867653665946385	18.13825121413748
49	14.342714947657456	12.51072593101081	28.65539728848464	44.49116183284709
50	17.529462181272766	18.219412899078637	45.30533533319049	18.94578958645811
51	22.46487182237477	18.182988308484394	39.47227287353856	19.879866995602274
52	16.971778087777658	23.85234467217513	40.48073827663912	18.69513896340809
53	22.42576166602391	17.88576080901573	40.39079573209924	19.29768179286112
54	15.649623158615963	16.336930455635493	41.54033915724563	26.47310722850291
55	17.900824676020136	17.43814929848988	40.244189782585416	24.416836242904573
56	23.43438671665774	18.84092126405999	36.25709694697375	21.467595072308516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.5
13	8.5
14	14.0
15	20.5
16	27.0
17	45.5
18	64.0
19	93.0
20	122.0
21	177.0
22	267.5
23	303.0
24	394.5
25	486.0
26	601.0
27	716.0
28	888.0
29	1224.5
30	1389.0
31	1536.5
32	1684.0
33	1729.0
34	1774.0
35	1701.0
36	1628.0
37	1418.5
38	1124.0
39	1039.0
40	877.0
41	715.0
42	698.0
43	681.0
44	677.0
45	673.0
46	729.0
47	845.5
48	906.0
49	1148.0
50	1390.0
51	1541.5
52	1693.0
53	1735.0
54	1840.0
55	1903.0
56	1971.0
57	2039.0
58	2168.5
59	2298.0
60	2487.5
61	2677.0
62	3001.0
63	3458.5
64	3592.0
65	3302.0
66	3012.0
67	2665.5
68	2319.0
69	2018.5
70	1718.0
71	1441.0
72	989.0
73	814.0
74	723.0
75	632.0
76	522.5
77	413.0
78	335.5
79	198.0
80	138.0
81	97.0
82	56.0
83	41.5
84	27.0
85	18.0
86	9.0
87	5.5
88	1.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.02565911860927577
10	0.0
11	0.0
12	0.0
13	0.0
14	0.021382598841063142
15	0.16892253084439884
16	0.029935638377488402
17	0.017106079072850514
18	0.008553039536425257
19	0.012829559304637885
20	0.032073898261594715
21	0.008553039536425257
22	0.0
23	0.0
24	0.0
25	0.0
26	0.15823123142386727
27	0.008553039536425257
28	0.14112515235101675
29	0.029935638377488402
30	0.01924433895695683
31	0.18816686980135566
32	0.012829559304637885
33	0.149678191887442
34	0.07270083605961468
35	0.047041717450338916
36	0.1368486325828041
37	0.22024076806295037
38	0.1325721128145915
39	0.3378450616887977
40	0.5623623495199607
41	0.8510274338743131
42	0.1817520901490367
43	0.32287724250005345
44	0.47683195415570806
45	0.12829559304637886
46	0.09408343490067783
47	0.03635041802980734
48	0.055594756986764166
49	0.32287724250005345
50	0.20741120875831248
51	0.32501550238415977
52	0.3677807000662861
53	0.1988581692218872
54	0.13471037269869782
55	0.17533731049671777
56	0.1967199093377809
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	46767.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.46624756772938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.25243932949712	79.69936066029464
2	4.415811858894171	7.5480573908952895
3	1.075806855141356	2.7583552504971456
4	0.4903677758318739	1.6763957491393504
5	0.2551913935451589	1.0905125408942202
6	0.12509382036527394	0.6414779652318943
7	0.0725544158118589	0.43406675647358184
8	0.0475356517388041	0.32501550238415977
9	0.05003752814610958	0.3848867791391366
>10	0.17763322491868902	2.801120448179272
>50	0.03252439329497123	2.136121624222208
>100	0.005003752814610959	0.5046293326490902
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	124	0.265144225629183	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	112	0.23948510701990722	No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG	97	0.20741120875831248	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC	96	0.20527294887420616	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	95	0.20313468899009987	No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG	87	0.18602860991724934	No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG	81	0.17319905061261145	No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG	81	0.17319905061261145	No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG	81	0.17319905061261145	No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG	76	0.16250775119207989	No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG	72	0.15395471165565464	No Hit
GCAGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG	70	0.149678191887442	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTC	58	0.12401907327816623	No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG	53	0.11332777385763466	No Hit
GCAGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG	52	0.11118951397352833	No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG	50	0.1069129942053157	No Hit
GCAGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	47	0.10049821455299678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.002138259884106314	0.0	0.0	0.0	0.0
7	0.002138259884106314	0.0	0.0	0.0	0.0
8	0.002138259884106314	0.0	0.0	0.0	0.0
9	0.002138259884106314	0.0	0.0	0.0	0.0
10	0.006414779652318943	0.0	0.0	0.0	0.0
11	0.006414779652318943	0.0	0.0	0.0	0.0
12	0.008553039536425257	0.0	0.0	0.0	0.0
13	0.012829559304637885	0.0	0.0	0.0	0.0
14	0.0149678191887442	0.0	0.0	0.0	0.0
15	0.0149678191887442	0.0	0.0	0.0	0.0
16	0.017106079072850514	0.0	0.0	0.0	0.0
17	0.021382598841063142	0.0	0.0	0.0	0.0
18	0.021382598841063142	0.0	0.0	0.0	0.0
19	0.023520858725169458	0.0	0.0	0.0	0.0
20	0.023520858725169458	0.0	0.0	0.0	0.0
21	0.02565911860927577	0.0	0.0	0.0	0.0
22	0.03421215814570103	0.0	0.0	0.0	0.0
23	0.03635041802980734	0.0	0.0	0.0	0.0
24	0.03635041802980734	0.0	0.0	0.0	0.0
25	0.04062693779801997	0.0	0.0	0.0	0.0
26	0.042765197682126284	0.0	0.0	0.0	0.0
27	0.04917997733444523	0.0	0.0	0.0	0.0
28	0.04917997733444523	0.0	0.0	0.0	0.0
29	0.05131823721855154	0.0	0.0	0.0	0.0
30	0.05131823721855154	0.0	0.0	0.0	0.0
31	0.05345649710265786	0.0	0.0	0.0	0.0
32	0.05345649710265786	0.0	0.0	0.0	0.0
33	0.05345649710265786	0.0	0.0	0.0	0.0
34	0.05345649710265786	0.0	0.0	0.0	0.0
35	0.05345649710265786	0.0	0.0	0.0	0.0
36	0.057733016870870485	0.0	0.0	0.0	0.0
37	0.057733016870870485	0.0	0.0	0.0	0.0
38	0.057733016870870485	0.0	0.0	0.0	0.0
39	0.057733016870870485	0.0	0.0	0.0	0.0
40	0.0598712767549768	0.0	0.0	0.0	0.0
41	0.0598712767549768	0.0	0.0	0.0	0.0
42	0.06200953663908312	0.0	0.0	0.0	0.0
43	0.06414779652318943	0.0	0.0	0.0	0.0
44	0.06842431629140205	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTTC	15	0.007968481	50.64091	38
ACTTCAG	15	0.007968481	50.64091	40
CTGTCGA	15	0.007968481	50.64091	40
CAAACCA	30	9.396008E-7	50.64091	39
CCGGTCA	15	0.007968481	50.64091	40
TGTCGAC	15	0.008002866	50.58587	41
AGCCCCT	15	0.008106683	50.421448	36
TACAAAC	15	0.008106683	50.421448	37
AGAAACT	15	0.008106683	50.421448	36
CTTATCT	15	0.008211499	50.2581	44
CGACTCC	15	0.008211499	50.2581	44
CCTGGCG	15	0.008211499	50.2581	44
TTCACCC	20	4.0815113E-4	50.149788	47
GTTCACC	20	4.0815113E-4	50.149788	46
TCAGTTC	20	4.0815113E-4	50.149788	43
CCCGCTC	20	4.0815113E-4	50.149788	47
GCTCTGA	20	4.0815113E-4	50.149788	46
TCTCACG	35	5.023503E-8	50.149788	48
CCAAAAC	25	2.020302E-5	50.149784	43
CCGCTCT	45	1.2732926E-10	50.149784	48
>>END_MODULE