FastQCFastQC Report
Fri 17 Jun 2016
SRR1526353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35483
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC840.23673308344841193No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC650.18318631457317588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAT150.0082120550.23186549
CATTTGA150.0082120550.23186546
GCGCCGG150.0082120550.23186546
ACGGGCC150.0082120550.23186548
ATTTGAT150.0082120550.23186547
CGTGCAA150.0082120550.23186548
CGCCGGA150.0082120550.23186547
ATCCCGT204.035177E-450.2318648
GCAGCAT204.035177E-450.2318642
TCTGATC204.035177E-450.2318644
AGCATTT204.035177E-450.2318644
CTCTGAT204.063658E-450.1605143
CCTCAGC150.00825841650.1605143
CTGATCC204.0922986E-450.08936345
CACTATC204.0922986E-450.08936333
GCATTTG150.00830497750.08936345
CTACCCG150.00830497750.08936334
ACCACAA150.00835173250.01841411
GAAGCGT150.00835173250.0184149
AAGAGTG252.0417947E-550.01841410
Produced by FastQC (version 0.11.5)