##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526353_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35483 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.440267170194176 39.0 38.0 39.0 34.0 40.0 2 36.910125975819405 39.0 38.0 39.0 33.0 40.0 3 36.85384550348054 39.0 37.0 39.0 33.0 40.0 4 36.80889440013527 39.0 38.0 40.0 33.0 40.0 5 36.75097934222022 39.0 38.0 39.0 33.0 40.0 6 36.699489896570185 39.0 38.0 39.0 33.0 40.0 7 36.35408505481498 39.0 36.0 39.0 31.0 40.0 8 36.312262210072426 39.0 36.0 39.0 31.0 40.0 9 36.288363441648116 39.0 36.0 39.0 31.0 40.0 10 36.12121297522757 39.0 35.0 39.0 31.0 40.0 11 34.20499957726235 37.0 32.0 39.0 26.0 40.0 12 32.78916664318124 37.0 31.0 39.0 17.0 40.0 13 32.509624327142575 37.0 30.0 39.0 16.0 40.0 14 32.20423864949412 37.0 29.0 39.0 15.0 40.0 15 32.651438717132145 37.0 30.0 39.0 17.0 40.0 16 32.435532508525206 36.0 30.0 39.0 17.0 40.0 17 32.40577177803455 36.0 30.0 39.0 17.0 40.0 18 32.51819181016261 36.0 30.0 39.0 18.0 40.0 19 33.49260209114224 36.0 31.0 39.0 24.0 40.0 20 33.66936279345038 37.0 31.0 39.0 24.0 40.0 21 34.92996646281318 38.0 35.0 39.0 26.0 40.0 22 34.56204379562044 38.0 34.0 39.0 25.0 40.0 23 34.83256770848012 38.0 35.0 39.0 27.0 40.0 24 34.48992475269848 38.0 33.0 39.0 25.0 40.0 25 34.720570414001074 38.0 34.0 39.0 26.0 40.0 26 34.95316066848913 38.0 35.0 39.0 27.0 40.0 27 34.843925259983656 38.0 35.0 39.0 27.0 40.0 28 34.806611616830594 38.0 35.0 39.0 27.0 40.0 29 34.6614153256489 38.0 35.0 39.0 26.0 40.0 30 34.4717188512809 38.0 35.0 39.0 25.0 40.0 31 34.207197813037226 38.0 34.0 39.0 24.0 40.0 32 33.13541696023448 38.0 33.0 39.0 17.0 40.0 33 34.374320096947834 38.0 35.0 39.0 25.0 40.0 34 34.05554772708057 38.0 34.0 39.0 23.0 40.0 35 34.07183721782262 38.0 34.0 39.0 24.0 40.0 36 32.13930614660541 35.0 31.0 38.0 18.0 39.0 37 31.868331313586786 35.0 31.0 38.0 16.0 39.0 38 31.135642420313953 35.0 30.0 38.0 10.0 39.0 39 29.07451455626638 34.0 26.0 38.0 4.0 39.0 40 24.860750218414452 30.0 11.0 37.0 4.0 39.0 41 21.68148127272215 25.0 8.0 33.0 4.0 37.0 42 25.48234365752614 30.0 19.0 34.0 4.0 37.0 43 21.63512104388017 26.0 7.0 33.0 4.0 37.0 44 22.573654989713383 27.0 8.0 33.0 4.0 37.0 45 25.201561311050362 30.0 19.0 34.0 4.0 37.0 46 25.0947214158893 30.0 12.0 36.0 4.0 38.0 47 24.419834850491785 30.0 8.0 36.0 4.0 38.0 48 24.416960234478484 30.0 9.0 36.0 4.0 38.0 49 21.767353380492068 26.0 4.0 35.0 4.0 38.0 50 24.275427669588254 30.0 4.0 35.0 4.0 38.0 51 25.679311219457205 32.0 4.0 38.0 4.0 39.0 52 25.506101513400782 32.0 4.0 38.0 4.0 39.0 53 25.12749767494293 32.0 4.0 38.0 4.0 39.0 54 24.169461432235156 31.0 4.0 38.0 4.0 39.0 55 24.4127892230082 31.0 4.0 38.0 4.0 39.0 56 23.449031930783757 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4778355646844474 1 2 2.3431459194349813 1 3 2.5056734044403797 1 4 2.6131940495000876 1 5 2.5815069339554455 1 6 2.7893016999921443 1 7 3.2830728305979164 1 8 3.2362834759498753 1 9 3.2919833529802602 1 10 2.957599864345987 1 11 -1.9541733140637945 1 12 -3.990710299751413 1 13 -19.01741546073692 1 14 -15.21427582129189 1 15 -6.938782284594392 1 16 -18.95708511462348 1 17 -19.755606767455383 1 18 -19.64683925705266 1 19 -5.238953765816927 1 20 -9.585462876892727 1 21 -4.36048832875613 1 22 -0.6572994341788458 1 23 -2.020863309988684 1 24 -2.1171706781784962 1 25 -2.0668689717421174 1 26 3.177060464130399 1 27 3.374690800449642 1 28 3.355431918730403 1 29 3.7870354245906626 1 30 4.000607048976136 1 31 3.0665088204234436 1 32 -8.427519155020658 1 33 2.7360441356348204 1 34 3.3575623619966066 1 35 3.315139355376438 1 36 6.132089701618881 1 37 6.216518431031911 1 38 6.896043965539505 1 39 9.865496419737376 1 40 12.776307754236033 1 41 5.770835621924622 1 42 0.2991145091769525 1 43 -10.831515070169129 1 44 -14.843183607446427 1 45 -9.328008516242162 1 46 -16.901769940213732 1 47 -16.796853337634605 1 48 -14.640591313319657 1 49 -13.683199156047014 1 50 -14.017180342920437 1 51 -15.84242460136718 1 52 -16.321151627876077 1 53 -16.17580967887458 1 54 -17.13149030485534 1 55 -15.926011832688822 1 56 -14.969533116613608 2 1 1.1119819061478609 2 2 1.7856546302363725 2 3 1.4549018763169315 2 4 1.7580422326757983 2 5 1.921974828430308 2 6 1.9545580464331636 2 7 2.387104687243017 2 8 2.650419862211699 2 9 2.718563741233119 2 10 2.34187064582931 2 11 -3.069155892461005 2 12 -10.5790627138878 2 13 -11.467390572732938 2 14 -16.02014939023166 2 15 -7.477856450846257 2 16 -2.897353905066492 2 17 -1.6660099531198895 2 18 -9.711050307326431 2 19 -1.6640408738308672 2 20 -2.6033822397598243 2 21 2.869974588117934 2 22 3.7732630347111495 2 23 2.5734622251034054 2 24 3.4746660565153817 2 25 2.9634943931160223 2 26 3.6021475721443323 2 27 3.367224399255015 2 28 3.5968455573565805 2 29 3.0861892324552755 2 30 3.3609853466366673 2 31 -0.8359302039668037 2 32 -8.062661016643357 2 33 0.14769172149843257 2 34 1.1206285640871982 2 35 0.4216600124197498 2 36 6.013125042584541 2 37 5.933292945715831 2 38 6.554082790825717 2 39 9.07704445358506 2 40 12.3417632047089 2 41 8.410457324264094 2 42 4.252325061690641 2 43 -2.5816395101890386 2 44 -8.634124373996949 2 45 3.2902095026727203 2 46 -9.075488208008654 2 47 -10.152254034498716 2 48 -4.411621676684515 2 49 -9.787728772771752 2 50 -6.243661179157369 2 51 -4.621419126006302 2 52 -5.009056057940786 2 53 -5.297263138307134 2 54 -14.073252375537272 2 55 -4.88270670576 2 56 -3.447880553149197 3 1 1.2369819061478609 3 2 1.232083201664949 3 3 0.783983508969996 3 4 1.6432463143084561 3 5 1.381158501899698 3 6 1.783639679086221 3 7 2.3845536668348544 3 8 2.147868841803536 3 9 2.3869310881718917 3 10 2.00768697235992 3 11 1.7165583932532797 3 12 2.852059735091789 3 13 -3.1485130217125317 3 14 -5.275251431047987 3 15 -2.6156115528870743 3 16 2.9087685439131015 3 17 2.2038880060637815 3 18 2.2455823457347925 3 19 1.1752448404548517 3 20 1.2869238826891518 3 21 3.068954179954673 3 22 3.4084671163438074 3 23 3.244380592450341 3 24 3.5078293218215038 3 25 3.315535209442551 3 26 3.137861857858617 3 27 2.8468162359897136 3 28 2.6376618838871977 3 29 3.1550667834756823 3 30 2.9400669792897247 3 31 3.9854983674617657 3 32 4.057236942540317 3 33 3.5252427419066024 3 34 4.013485706944337 3 35 4.289006951195255 3 36 5.546288307890663 3 37 6.124619476328082 3 38 6.997960341846124 3 39 8.60765669848302 3 40 12.079008102668087 3 41 11.349232834468175 3 42 9.60181485760901 3 43 4.306115591851778 3 44 4.740875626003049 3 45 9.708576849611497 3 46 2.319919955256651 3 47 -1.6522540344987142 3 48 2.101133425356302 3 49 -8.01987162991461 3 50 2.9706245351283442 3 51 1.0648053637896133 3 52 1.9118623094061533 3 53 0.3660021678153136 3 54 -4.4354972734964555 3 55 0.6070892126073453 3 56 2.9220174060344775 4 1 1.8016370785616544 4 2 2.618166945507312 4 3 1.4102150360635903 4 4 2.642630550761652 4 5 2.632390028993292 4 6 2.4911519943571605 4 7 3.4504403663422423 4 8 3.3590757383552585 4 9 3.36599512758076 4 10 2.638844607827906 4 11 4.5613859794601765 4 12 4.7245966809046 4 13 6.502349047252984 4 14 4.1040589137796 4 15 6.082664309181894 4 16 7.27083750943034 4 17 6.465587513452942 4 18 6.377971508296373 4 19 5.648767007942531 4 20 5.6496086117531945 4 21 3.969200485373392 4 22 4.285930170530996 4 23 4.041794385553786 4 24 4.417312080442194 4 25 4.149279051807085 4 26 3.082443138646795 4 27 2.962579782787742 4 28 2.8642628691088703 4 29 3.6667662908648424 4 30 3.9320620531813546 4 31 3.336483589136641 4 32 5.078788666678246 4 33 3.296178702497734 4 34 4.078756642904928 4 35 4.252061138387376 4 36 6.1922242684817945 4 37 5.83090026450542 4 38 7.331704184210658 4 39 9.812090196019966 4 40 10.877653422865134 4 41 5.7876564797883745 4 42 11.124598108840534 4 43 9.345524458846853 4 44 13.230407645707487 4 45 11.415473401335632 4 46 12.193688428163057 4 47 11.931489807865816 4 48 11.727364952449896 4 49 8.788625907031204 4 50 11.694762466162828 4 51 10.528475314528528 4 52 10.991295806943096 4 53 10.494080985549306 4 54 4.479527357045416 4 55 9.929749311129513 4 56 9.673864696674869 5 1 1.2905533347192915 5 2 1.9999403445220878 5 3 1.9446977946842807 5 4 2.428960600022741 5 5 1.3454442161854132 5 6 3.015782536229082 5 7 2.795267952549139 5 8 2.665725984660682 5 9 2.6369310881718917 5 10 2.418401258074205 5 11 4.323701250396134 5 12 5.512774020806074 5 13 6.512201264001753 5 14 6.2426057118091585 5 15 6.812959875684356 5 16 6.801625686770247 5 17 5.632459434635212 5 18 5.6205823457347925 5 19 5.01453055474056 5 20 5.019066739832013 5 21 2.801097037097527 5 22 3.8727528306295227 5 23 3.6550948781646255 5 24 3.5078293218215038 5 25 4.0119637808711275 5 26 2.6021475721443323 5 27 2.8825305217039983 5 28 2.4055190267443365 5 29 3.3872096406185435 5 30 2.922209836432586 5 31 3.967641224604627 5 32 3.967951228254602 5 33 3.2216713133351718 5 34 2.799199992658629 5 35 3.87829266548097 5 36 5.242716879319232 5 37 5.517476619185221 5 38 5.176531770417554 5 39 8.018370984197304 5 40 6.525436674096657 5 41 3.652804263039606 5 42 9.10181485760901 5 43 13.52040130613749 5 44 12.062304197431622 5 45 8.726433992468639 5 46 8.819919955256651 5 47 9.669174536929855 5 48 9.636847711070587 5 49 10.605128370085389 5 50 10.452767392271202 5 51 10.029091078075332 5 52 10.251148023691865 5 53 9.133859310672456 5 54 10.010931297932114 5 55 8.874946355464488 5 56 9.529160263177332 6 1 1.6402837929403162 6 2 2.4379457353576726 6 3 1.3597921343069217 6 4 2.936709926168291 6 5 2.5064954291234187 6 6 3.0373458785471428 6 7 3.0971547450019727 6 8 3.4143782757657988 6 9 2.7609203065007364 6 10 2.7169187782898447 6 11 4.801464053630639 6 12 6.910348144795297 6 13 6.207619053759167 6 14 7.224748568952013 6 15 6.9457092018299065 6 16 6.823862883535742 6 17 7.123362399594779 6 18 6.8018491920959825 6 19 4.8130480749562 6 20 5.8425168746029 6 21 3.8742102446446935 6 22 4.70395229154596 6 23 4.407116441506943 6 24 4.1092444161611255 6 25 3.607314185183796 6 26 3.634492585621423 6 27 2.128487395019363 6 28 2.133618757202562 6 29 4.003786460025552 6 30 3.2739618579959284 6 31 4.088598097919991 6 32 4.710538829332773 6 33 4.3352157877287 6 34 4.689361717726008 6 35 4.576068945804423 6 36 4.693525504656158 6 37 4.078460446678481 6 38 5.676531770417554 6 39 6.43245454214879 6 40 2.5982129544201094 6 41 -1.5402550361517697 6 42 7.668189520681786 6 43 12.288595376218353 6 44 10.654285329507093 6 45 8.793482509988856 6 46 11.262979254448027 6 47 12.84235512992177 6 48 12.205243937485683 6 49 14.190707884910186 6 50 12.055530195505703 6 51 12.011233935218186 6 52 12.687131851185125 6 53 12.221797316063292 6 54 12.336066068821605 6 55 12.143477352769068 6 56 11.999173740266283 7 1 1.650443444609401 7 2 1.7169733115550585 7 3 1.310082410068894 7 4 2.721543017605157 7 5 2.5130266337678293 7 6 2.513035283481827 7 7 3.256806414087599 7 8 3.0805611494958427 7 9 3.0682497694905777 7 10 2.753566093239044 7 11 4.8511737778686665 7 12 6.559477317509373 7 13 7.168794670595162 7 14 6.878594722798166 7 15 6.0574653701898455 7 16 7.485691620836182 7 17 6.48548141265719 7 18 6.661791136943588 7 19 4.484310774520786 7 20 5.304781025546298 7 21 3.9453278063282937 7 22 3.72028030315699 7 23 3.157842130911881 7 24 3.8443677833599637 7 25 2.763337407244748 7 26 2.280719000715763 7 27 2.530882170055648 7 28 2.209090455315767 7 29 3.725121728530631 7 30 2.784847199069951 7 31 3.010223642187043 7 32 4.028390788694164 7 33 3.8823855990494565 7 34 3.617881311339943 7 35 1.4291168413051452 7 36 0.9130465496489002 7 37 1.7221469488555492 7 38 1.6957625396483245 7 39 3.78622812705445 7 40 -2.3372006885407046 7 41 -9.071096835861495 7 42 4.050990681784835 7 43 8.73743427317046 7 44 6.764227274354699 7 45 3.9585768496114966 7 46 5.966897977234673 7 47 7.449394317149636 7 48 5.723386172609047 7 49 9.099633864590885 7 50 6.816778381282191 7 51 7.91508008906434 7 52 6.869279891823734 7 53 7.695672497485646 7 54 7.234832396833212 7 55 7.906539762057896 7 56 7.437127296144364 8 1 1.930163724329681 8 2 2.7103299549116997 8 3 1.9992432492297354 8 4 2.9435709896331304 8 5 2.930509151250348 8 6 2.9014968219433683 8 7 3.3589043161855017 8 8 3.5546870236217174 8 9 3.5070609583017642 8 10 2.8476220372949896 8 11 5.39837657507146 8 12 7.101085709117765 8 13 7.262850614651107 8 14 7.333839478042922 8 15 7.225297538022019 8 16 7.536041271185827 8 17 7.147719174894949 8 18 6.903049878202328 8 19 5.016478606688615 8 20 5.732053752819027 8 21 4.103369764370257 8 22 4.182168415045105 8 23 4.351198774268525 8 24 3.864647503639688 8 25 4.094106638013983 8 26 3.376173546170307 8 27 3.5494136385871116 8 28 3.390908637133947 8 29 3.5307161341250364 8 30 3.81701503123778 8 31 3.7640697960331977 8 32 5.200418760722133 8 33 4.0460219626858205 8 34 4.6608883043469405 8 35 3.763032925221232 8 36 1.5147948013971515 8 37 2.443125969834572 8 38 2.894713588599373 8 39 4.854409945236267 8 40 -1.6330048843448992 8 41 -10.118299633064291 8 42 6.689801870596021 8 43 10.930790916527101 8 44 10.500940561067985 8 45 7.644940485975134 8 46 11.095569305906004 8 47 11.094499212254533 8 48 10.770938620161495 8 49 13.708375123332143 8 50 11.37971544421925 8 51 11.720324844309097 8 52 12.21543373797758 8 53 11.143924245737391 8 54 13.272944284945101 8 55 13.15514116065929 8 56 10.898316107333176 9 1 1.7919819061478606 9 2 2.34851177309352 9 3 0.6646977946842796 9 4 2.7253891714513117 9 5 2.9268727876139806 9 6 2.801496821943367 9 7 3.1952679525491376 9 8 2.4128688418035367 9 9 2.9797882310290404 9 10 2.811258400931351 9 11 5.1365583932532815 9 12 7.275631163663217 9 13 7.426486978287471 9 14 7.424748568952015 9 15 7.194388447112928 9 16 6.928768543913105 9 17 5.893173720349498 9 18 6.304868060020507 9 19 4.967387697597708 9 20 4.8647810255463 9 21 3.6960970370975303 9 22 4.114895687772375 9 23 3.8293805924503417 9 24 3.5428293218215003 9 25 3.07410663801398 9 26 3.1107190007157612 9 27 2.6439590931325654 9 28 2.0290904553157674 9 29 2.894352497761396 9 30 3.847924122146871 9 31 1.5440697960331988 9 32 4.2022369425403205 9 33 2.302385599049458 9 34 2.186342849801484 9 35 2.2375783797666884 9 36 1.6184311650335204 9 37 1.8867623334709371 9 38 2.3365317704175546 9 39 4.676228127054451 9 40 -1.3202776116176267 9 41 -3.4364814512461095 9 42 5.482529143323294 9 43 9.408972734708922 9 44 8.077304197431623 9 45 5.308576849611498 9 46 9.299205669542367 9 47 10.256317394072713 9 48 10.041847711070588 9 49 12.346556941513963 9 50 10.270624535128345 9 51 8.131233935218187 9 52 9.175433737977581 9 53 8.280287882101028 9 54 9.140217012217828 9 55 7.8642320697502015 9 56 6.665588834605906 10 1 1.7465972907632477 10 2 2.697742542324285 10 3 1.7523901023765873 10 4 2.760004556066697 10 5 2.609180479921676 10 6 2.9938045142510603 10 7 3.1606525679337523 10 8 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29 3.0300667834756823 115 30 2.0650669792897247 115 31 2.806926938890342 115 32 -2.8891916288882555 115 33 2.3823855990494565 115 34 2.9420571355157676 115 35 3.0390069511952547 115 36 3.7427168793192322 115 37 3.5710480477566513 115 38 6.3193889132747 115 39 7.679085269911596 115 40 10.811150959810941 115 41 8.170661405896748 115 42 2.6911005718947223 115 43 1.5561155918517784 115 44 0.29444705457447995 115 45 5.422862563897212 115 46 1.5877770981137935 115 47 -5.509396891641572 115 48 -1.0952951460722709 115 49 -5.037728772771752 115 50 1.756338820842629 115 51 4.689805363789613 115 52 5.179719452263296 115 53 4.258859310672456 115 54 4.3145027265035445 115 55 3.6070892126073453 115 56 6.0470174060344775 116 1 1.3377883577607648 116 2 1.9285117730935184 116 3 1.783407472103633 116 4 2.2221633649996946 116 5 1.779776013420431 116 6 1.7208516606530466 116 7 2.6984937590007547 116 8 2.6438365837390165 116 9 2.2636591987709735 116 10 1.9725487235119985 116 11 -2.0415061228757523 116 12 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118 15 -15.265611552887075 118 16 -7.1912314560869 118 17 -11.186826279650504 118 18 -5.155131939979491 118 19 -5.252612302402294 118 20 -3.595218974453701 118 21 0.45609703709752836 118 22 1.5748956877723757 118 23 0.7293805924503403 118 24 0.0828293218215066 118 25 1.6941066380139844 118 26 2.1107190007157612 118 27 2.4039590931325705 118 28 2.40909045531577 118 29 2.814352497761398 118 30 3.06792412214687 118 31 -0.03593020396680657 118 32 -9.097763057459684 118 33 1.3223855990494542 118 34 2.406342849801483 118 35 -0.5024216202333136 118 36 3.9184311650335175 118 37 4.166762333470938 118 38 4.276531770417556 118 39 6.836228127054447 118 40 10.119722388382375 118 41 6.623518548753889 118 42 1.562529143323296 118 43 -3.6110272652910798 118 44 -3.382695802568378 118 45 0.6085768496114952 118 46 -4.300794330457634 118 47 -7.563682605927287 118 48 -6.858152288929412 118 49 -6.793443058486039 118 50 -4.729375464871655 118 51 -3.068766064781812 118 52 -2.42456626202242 118 53 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119 36 3.4867644983668526 119 37 4.315095666804272 119 38 3.8431984370842187 119 39 5.327894793721114 119 40 7.90638905504904 119 41 9.68851854875389 119 42 3.9708624766566274 119 43 3.3239727347089207 119 44 -0.4376958025683777 119 45 4.208576849611497 119 46 -2.0491276637909657 119 47 -4.807015939260619 119 48 -6.154818955596079 119 49 -5.9901097251527045 119 50 -2.8210421315383236 119 51 -1.6554327314484816 119 52 -2.9512329286890875 119 53 -6.878045451232305 119 54 -9.608116321115505 119 55 -7.785767930249797 119 56 -4.3994111653940955 120 1 -0.40801809385214227 120 2 0.20851177309351954 120 3 0.10469779468427731 120 4 1.2453891714513077 120 5 1.246872787613981 120 6 0.7014968219433655 120 7 0.7152679525491408 120 8 0.5328688418035341 120 9 0.21978823102903533 120 10 -0.22874159906864833 120 11 -10.383441606746722 120 12 -2.5643688363367794 120 13 -8.573513021712532 120 14 -3.6152514310479873 120 15 -6.745611552887073 120 16 -10.631231456086898 120 17 -9.706826279650503 120 18 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-8.334411165394096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 657.0 5 85.0 6 12.0 7 51.0 8 110.0 9 96.0 10 61.0 11 55.0 12 68.0 13 78.0 14 94.0 15 116.0 16 174.0 17 214.0 18 275.0 19 392.0 20 407.0 21 530.0 22 625.0 23 745.0 24 903.0 25 1035.0 26 1030.0 27 1119.0 28 1288.0 29 1354.0 30 1457.0 31 1691.0 32 1978.0 33 2382.0 34 2797.0 35 3375.0 36 3763.0 37 3745.0 38 2121.0 39 600.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.75996956288927 1.1047543894259222 0.04791026688836908 0.08736578079643773 8 98.1399543443339 1.3865794887692697 0.2649155933827467 0.20855057351407716 9 92.88231148696265 4.6060606060606055 1.4855532064834391 1.026074700493305 10 49.43775892681002 32.4211594284587 7.995378068370769 10.14570357636051 11 35.52223662702215 24.91685925257877 20.342708979200722 19.218195141198354 12 33.06372065496153 14.446354592339993 23.459121269340248 29.030803483358227 13 23.88185891835527 14.234985767832484 26.66629089986754 35.216864413944705 14 20.850248082995037 16.00417230491655 30.074424898511502 33.0711547135769 15 26.32514817950889 17.504939316963025 26.97713801862828 29.192774484899804 16 30.140114459699475 19.286177440726227 24.143666657269318 26.43004144230498 17 26.736942980354577 21.748076326841232 24.51872938921616 26.996251303588036 18 29.021166257997237 24.179138130266903 22.251345790704885 24.548349821030975 19 26.553911205073994 27.22480620155039 22.170542635658915 24.0507399577167 20 29.187573997857584 24.384055928285505 22.007667587528896 24.420702486328015 21 28.275084554678692 23.052423900789176 25.011273957158963 23.66121758737317 22 26.426739565425695 22.461460417664796 27.151030070738102 23.960769946171407 23 27.861229321083336 22.097906039511876 26.863568469407884 23.1772961699969 24 26.25200800383282 23.667671842854325 25.116252853479132 24.964067299833722 25 25.347349434940675 24.79215398923428 25.037341825662995 24.82315475016205 26 23.415487987352137 23.342085203692726 29.46557127127975 23.776855537675388 27 23.365750528541227 20.101479915433405 29.25722339675828 27.27554615926709 28 23.155755060557297 22.949662629513565 28.994099545467378 24.90048276446176 29 24.41857187156429 21.932173765962844 30.721957545175204 22.927296817297663 30 22.840550293188993 20.300518718989625 34.61039693279206 22.248534055029317 31 25.295895596169597 19.527696957713058 33.414310330216665 21.76209711590068 32 26.192220969560314 18.015783540022547 32.581736189402484 23.21025930101466 33 26.452396418787245 19.64583274493744 35.23597028836106 18.665800547914255 34 21.16399131097131 20.9862611786611 38.41227748469546 19.43747002567213 35 20.773655125747155 22.71061238299312 39.02672831848427 17.48900417277546 36 22.011122716878866 24.35987917454761 35.810066905682746 17.818931202890777 37 23.004973437323386 22.35220978862891 34.489092347688484 20.15372442635922 38 23.15117985773964 23.82296488653043 35.353392796658014 17.67246245907192 39 21.894981044531207 18.61879703502518 31.729191421943074 27.75703049850054 40 14.108342546134875 14.62709414065822 26.892876378376844 44.37168693483006 41 16.09950248756219 17.165600568585642 28.28997867803838 38.44491826581379 42 18.79943502824859 21.98870056497175 37.17796610169491 22.033898305084744 43 12.901857663923996 12.986682500636187 29.22781123646337 44.88364859897644 44 14.244844776795832 14.242012236573759 27.58044414230682 43.93269884432359 45 23.004429897576255 20.33520498857256 36.257441945769024 20.402923168082165 46 20.76871016863121 22.627037392138064 36.23879081834076 20.365461620889967 47 17.902957512193744 20.11333840818743 34.6020468578195 27.381657221799315 48 20.59039133867148 21.041502199165446 39.511672493515285 18.856433968647796 49 14.920563125459378 15.211737434273761 26.87849833210833 42.98920110815854 50 19.78087143139525 21.424335695930875 38.192189309010814 20.60260356366306 51 19.125590339639718 21.998812250784763 37.39996040835949 21.47563700121603 52 19.868674289595834 22.231971017774256 36.091927997282916 21.807426695346994 53 19.00943129835658 22.17766984808268 38.62596713164285 20.186931721917887 54 17.66266012076068 19.451498222447945 34.648157553185484 28.237684103605893 55 19.904022582921666 22.108680310515172 36.35850388143966 21.6287932251235 56 21.50601389124174 22.025523744988423 35.19961601445593 21.268846349313907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 4.0 9 5.0 10 13.5 11 22.0 12 33.0 13 67.0 14 90.0 15 112.5 16 135.0 17 163.0 18 191.0 19 233.5 20 276.0 21 287.0 22 353.5 23 409.0 24 452.5 25 496.0 26 579.5 27 663.0 28 806.0 29 1034.0 30 1119.0 31 1140.5 32 1162.0 33 1136.5 34 1111.0 35 988.5 36 866.0 37 792.0 38 670.0 39 622.0 40 612.0 41 602.0 42 637.0 43 672.0 44 717.5 45 763.0 46 831.5 47 936.0 48 972.0 49 1036.5 50 1101.0 51 1191.5 52 1282.0 53 1363.0 54 1548.0 55 1652.0 56 1759.0 57 1866.0 58 2003.5 59 2141.0 60 2317.5 61 2494.0 62 2544.5 63 2359.0 64 2123.0 65 1928.5 66 1734.0 67 1520.0 68 1306.0 69 1088.0 70 870.0 71 749.0 72 562.5 73 497.0 74 391.5 75 286.0 76 247.5 77 209.0 78 153.5 79 72.5 80 47.0 81 31.5 82 16.0 83 10.5 84 5.0 85 2.5 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.022546007947467802 10 0.0 11 0.0028182509934334752 12 0.0 13 0.0 14 0.03100076092776823 15 0.14936730265197418 16 0.0338190119212017 17 0.011273003973733901 18 0.0056365019868669505 19 0.022546007947467802 20 0.025364258940901274 21 0.008454752980300425 22 0.0 23 0.0 24 0.0 25 0.0 26 0.17473156159287545 27 0.022546007947467802 28 0.17473156159287545 29 0.02818250993433475 30 0.03100076092776823 31 0.23109658146154496 32 0.008454752980300425 33 0.2141870755009441 34 0.1014570357636051 35 0.04227376490150213 36 0.1690950596060085 37 0.26773384437618014 38 0.15500380463884114 39 0.3861003861003861 40 0.5805597046472959 41 0.8680213059775104 42 0.23391483245497843 43 0.3269171152382831 44 0.5044669278245921 45 0.11836654172420596 46 0.05918327086210298 47 0.03945551390806865 48 0.04227376490150213 49 0.30718935828424876 50 0.19727756954034328 51 0.34382662119888396 52 0.4255559000084548 53 0.1944593185469098 54 0.11554829073077248 55 0.16345855761914155 56 0.18318631457317588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 35483.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.1891891891892 #Duplication Level Percentage of deduplicated Percentage of total 1 93.5633709356337 83.4484119155652 2 4.458558473157013 7.953104303469267 3 1.01747401017474 2.722430459656737 4 0.3918222896325086 1.3978524927430036 5 0.20223085916516573 0.901840317898712 6 0.09479571523367143 0.5072851788180255 7 0.06635700066357 0.41428289603472085 8 0.04107814326792429 0.2930981033170814 9 0.031598571744557144 0.25364258940901274 >10 0.12639428697822858 1.6881323450666514 >50 0.006319714348911429 0.41991939802158784 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 84 0.23673308344841193 No Hit AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 65 0.18318631457317588 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0028182509934334752 0.0 0.0 0.0 0.0 8 0.0028182509934334752 0.0 0.0 0.0 0.0 9 0.0028182509934334752 0.0 0.0 0.0 0.0 10 0.0056365019868669505 0.0 0.0 0.0 0.0 11 0.0056365019868669505 0.0 0.0 0.0 0.0 12 0.0056365019868669505 0.0 0.0 0.0 0.0 13 0.0056365019868669505 0.0 0.0 0.0 0.0 14 0.0056365019868669505 0.0 0.0 0.0 0.0 15 0.0056365019868669505 0.0 0.0 0.0 0.0 16 0.0056365019868669505 0.0 0.0 0.0 0.0 17 0.008454752980300425 0.0 0.0 0.0 0.0 18 0.008454752980300425 0.0 0.0 0.0 0.0 19 0.01690950596060085 0.0 0.0 0.0 0.0 20 0.01690950596060085 0.0 0.0 0.0 0.0 21 0.01690950596060085 0.0 0.0 0.0 0.0 22 0.01690950596060085 0.0 0.0 0.0 0.0 23 0.01690950596060085 0.0 0.0 0.0 0.0 24 0.025364258940901278 0.0 0.0 0.0 0.0 25 0.028182509934334753 0.0 0.0 0.0 0.0 26 0.028182509934334753 0.0 0.0 0.0 0.0 27 0.028182509934334753 0.0 0.0 0.0 0.0 28 0.028182509934334753 0.0 0.0 0.0 0.0 29 0.028182509934334753 0.0 0.0 0.0 0.0 30 0.031000760927768226 0.0 0.0 0.0 0.0 31 0.031000760927768226 0.0 0.0 0.0 0.0 32 0.0338190119212017 0.0 0.0 0.0 0.0 33 0.0338190119212017 0.0 0.0 0.0 0.0 34 0.0338190119212017 0.0 0.0 0.0 0.0 35 0.0338190119212017 0.0 0.0 0.0 0.0 36 0.0338190119212017 0.0 0.0 0.0 0.0 37 0.0338190119212017 0.0 0.0 0.0 0.0 38 0.0338190119212017 0.0 0.0 0.0 0.0 39 0.0338190119212017 0.0 0.0 0.0 0.0 40 0.0338190119212017 0.0 0.0 0.0 0.0 41 0.0338190119212017 0.0 0.0 0.0 0.0 42 0.0338190119212017 0.0 0.0 0.0 0.0 43 0.0338190119212017 0.0 0.0 0.0 0.0 44 0.03663726291463518 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCAAT 15 0.00821205 50.231865 49 CATTTGA 15 0.00821205 50.231865 46 GCGCCGG 15 0.00821205 50.231865 46 ACGGGCC 15 0.00821205 50.231865 48 ATTTGAT 15 0.00821205 50.231865 47 CGTGCAA 15 0.00821205 50.231865 48 CGCCGGA 15 0.00821205 50.231865 47 ATCCCGT 20 4.035177E-4 50.23186 48 GCAGCAT 20 4.035177E-4 50.23186 42 TCTGATC 20 4.035177E-4 50.23186 44 AGCATTT 20 4.035177E-4 50.23186 44 CTCTGAT 20 4.063658E-4 50.16051 43 CCTCAGC 15 0.008258416 50.16051 43 CTGATCC 20 4.0922986E-4 50.089363 45 CACTATC 20 4.0922986E-4 50.089363 33 GCATTTG 15 0.008304977 50.089363 45 CTACCCG 15 0.008304977 50.089363 34 ACCACAA 15 0.008351732 50.018414 11 GAAGCGT 15 0.008351732 50.018414 9 AAGAGTG 25 2.0417947E-5 50.018414 10 >>END_MODULE