FastQCFastQC Report
Fri 17 Jun 2016
SRR1526352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131004
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC3000.2290006412017954No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2360.1801471710787457No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1890.1442704039571311No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC1790.13663704925040457No Hit
GTGGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1320.10076028212878997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAA150.00800166250.63982840
ACTCACT150.00806338450.5415441
CGCTTTC150.00806338450.5415441
ATATGCC204.0381914E-450.32663734
CGAGACG204.084648E-450.21018244
TAGTGCT150.00827589250.21018244
CCGTCAC150.00832649350.13284743
CCGAATC150.00832649350.13284743
AGACTAC150.00836459550.07550
ACGGATA150.00837732450.05574833
TAGTTTG150.008428385549.9788928
ACACTTA204.1788354E-449.9788927
TGAGCAA150.008428385549.9788928
TAGCGGG150.00844118749.9597132
GTATAGT150.00849253849.8831425
GACATTG150.008518349.84494814
GATTGCG150.00853120349.825879
GGACAAT150.00853120349.825879
TAGTATA150.00853120349.8258723
AGTATCA150.0085441249.8068111
Produced by FastQC (version 0.11.5)