FastQCFastQC Report
Fri 17 Jun 2016
SRR1526351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34813
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCGG650.18671186051187774No Hit
ATACAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGG640.18383937035015657No Hit
ATACAGGGGTGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCGG550.1579869588946658No Hit
ATACAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC530.1522419785712234No Hit
ATACAGGGGTGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCGG530.1522419785712234No Hit
ATACAGGGGTGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCGG490.1407520179243386No Hit
ATACAGGGGTGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGG470.13500703760089622No Hit
ATACAGGGGTGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCGG440.12638956711573263No Hit
ATACAGGGGTGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCGG400.11489960646884785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTTA203.9409826E-450.4693937
TATTTAT203.9409826E-450.4693938
ATTTATT203.9409826E-450.4693939
TTCATTT150.00805868850.46938738
CAACCTG150.00805868850.46938741
TCATTTT150.00805868850.46938739
CAAACCA150.00805868850.46938739
TTTTCGT150.00810532350.39592436
TAGCAGC150.00810532350.39592435
TTGATAT150.00824643350.1768149
CATTTGA150.00829387350.104246
TGATATG150.00829387350.104250
ATTTGAT150.00829387350.104247
CGTTCCG402.5083864E-950.10419544
GTAGAGC150.00834151650.0317933
TGGTAGA150.00834151650.0317931
TGCGCAC150.00834151650.0317945
GTTTTGC150.00834151650.0317928
TGGCTCG252.0377158E-550.0317931
GGAGAAA252.0552585E-549.959620