##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526351_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34813 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.13158877430845 39.0 38.0 39.0 33.0 40.0 2 36.53336397322839 39.0 38.0 39.0 32.0 40.0 3 36.68497400396404 39.0 37.0 39.0 33.0 40.0 4 36.119639215235686 39.0 38.0 39.0 30.0 40.0 5 36.53207135265562 39.0 37.0 39.0 32.0 40.0 6 36.05308361818861 38.0 36.0 39.0 30.0 40.0 7 36.31660586562491 38.0 36.0 39.0 32.0 40.0 8 36.19237066613047 38.0 36.0 39.0 31.0 40.0 9 36.21374199293368 38.0 36.0 39.0 31.0 40.0 10 35.713095682647285 38.0 35.0 39.0 29.0 40.0 11 34.20567029557924 37.0 32.0 39.0 26.0 40.0 12 31.96426622238819 35.0 29.0 39.0 15.0 40.0 13 31.70648895527533 36.0 29.0 39.0 13.0 40.0 14 31.6119840289547 36.0 28.0 39.0 13.0 40.0 15 31.856634016028494 36.0 29.0 39.0 15.0 40.0 16 31.80033320885876 36.0 29.0 38.0 15.0 40.0 17 31.99905207824663 36.0 29.0 38.0 17.0 40.0 18 32.141125441645364 36.0 29.0 38.0 18.0 40.0 19 32.99155487892454 36.0 30.0 38.0 23.0 40.0 20 33.03966908913337 36.0 30.0 38.0 23.0 40.0 21 34.57863441817712 38.0 34.0 39.0 25.0 40.0 22 34.336856921265046 38.0 33.0 39.0 25.0 40.0 23 34.47806853761526 38.0 33.0 39.0 26.0 40.0 24 34.134116565650764 38.0 33.0 39.0 24.0 40.0 25 34.27550053141068 38.0 33.0 39.0 25.0 40.0 26 34.720334357854824 38.0 35.0 39.0 27.0 40.0 27 34.65432453393847 38.0 35.0 39.0 27.0 40.0 28 34.64303564760291 38.0 35.0 39.0 27.0 40.0 29 34.3871829488984 38.0 34.0 39.0 25.0 40.0 30 34.33651222244564 38.0 34.0 39.0 25.0 40.0 31 34.03811794444604 38.0 34.0 39.0 23.0 40.0 32 32.98394277999598 38.0 33.0 39.0 16.0 40.0 33 34.15387929796341 38.0 34.0 39.0 25.0 40.0 34 33.889006980151095 38.0 34.0 39.0 23.0 40.0 35 33.89529773360526 38.0 34.0 39.0 23.0 40.0 36 31.669089133369717 35.0 31.0 38.0 17.0 39.0 37 31.212937695688392 35.0 30.0 38.0 11.0 39.0 38 30.781518398299486 35.0 30.0 38.0 4.0 39.0 39 28.864906787694252 33.0 26.0 38.0 4.0 39.0 40 24.264728693304225 29.0 10.0 37.0 4.0 39.0 41 20.926148277942147 24.0 8.0 32.0 4.0 36.0 42 24.95366673369144 30.0 19.0 33.0 4.0 36.0 43 20.972309194841007 25.0 7.0 32.0 4.0 36.0 44 21.908798437365352 27.0 7.0 33.0 4.0 36.0 45 24.539597276879327 30.0 17.0 33.0 4.0 36.0 46 24.712894608335965 30.0 7.0 36.0 4.0 38.0 47 23.978628673196795 30.0 4.0 35.0 4.0 38.0 48 24.024157642260075 29.0 4.0 35.0 4.0 38.0 49 21.242380719846036 25.0 4.0 35.0 4.0 38.0 50 23.71749059259472 29.0 4.0 35.0 4.0 38.0 51 25.260534857668112 32.0 4.0 38.0 4.0 39.0 52 25.078706230431163 32.0 4.0 38.0 4.0 39.0 53 24.636945968460058 31.0 4.0 38.0 4.0 39.0 54 23.752161548846694 31.0 4.0 37.0 4.0 39.0 55 23.982190560997328 31.0 4.0 37.0 4.0 39.0 56 23.091747335765376 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.3882494869278972 1 2 2.4362737362087117 1 3 1.7656934670021514 1 4 2.4799903387197446 1 5 2.273461499154976 1 6 2.3240514247293476 1 7 2.423037162322153 1 8 3.003732031807239 1 9 2.5974562525427274 1 10 2.9500381909175672 1 11 -2.3235486332122655 1 12 -6.754086249640697 1 13 -19.678905702525178 1 14 -16.49245837970941 1 15 -10.342279141621919 1 16 -20.149825120864243 1 17 -20.756832400478856 1 18 -18.851269388319857 1 19 -5.315645126566086 1 20 -10.95389756383986 1 21 -6.461205210935592 1 22 -2.0824043272881845 1 23 -2.4225628866109474 1 24 -3.1409149874746163 1 25 -2.7382206649462546 1 26 2.8359876330988314 1 27 4.049752915299614 1 28 2.914508588699931 1 29 1.8115669067462363 1 30 2.7596742719432257 1 31 1.591295615537014 1 32 -10.17985500499464 1 33 0.06318759782784866 1 34 1.084059516543661 1 35 0.2674223634639361 1 36 3.2082472077983297 1 37 4.429642541070681 1 38 4.3594977568450055 1 39 6.536963515060638 1 40 10.328454151328934 1 41 1.2437900128784918 1 42 -4.414782612644313 1 43 -12.216384860056927 1 44 -14.755772839548982 1 45 -12.102620817254973 1 46 -17.658957168612204 1 47 -16.721317360267257 1 48 -14.711020974683223 1 49 -14.187470265833351 1 50 -13.86836742999029 1 51 -14.949272897980618 1 52 -15.123736494482525 1 53 -15.553267744468727 1 54 -16.676955056844804 1 55 -14.80896817594634 1 56 -13.698962009926468 2 1 1.7555850499156378 2 2 2.740621562295665 2 3 2.422884102453658 2 4 3.0898008180954406 2 5 2.648600852555205 2 6 3.2811305774606723 2 7 2.938087329546235 2 8 3.057243737492854 2 9 3.027779552431241 2 10 3.3641964963802238 2 11 -3.251642278697215 2 12 -9.765234521658531 2 13 -11.793175490708009 2 14 -13.22267019687775 2 15 -7.434252385769074 2 16 -3.1960904497382643 2 17 -1.7618491228868862 2 18 -9.729753223325428 2 19 -1.4399483595649691 2 20 -2.727587641877765 2 21 2.7383488581836914 2 22 3.19797471396042 2 23 2.7390870576476942 2 24 3.0519501184339646 2 25 2.9089365256891995 2 26 2.558395659854682 2 27 2.7465199164144423 2 28 2.6547538506843225 2 29 2.2981889803248308 2 30 3.0918927780747723 2 31 1.7306490157599796 2 32 -4.271270835540907 2 33 1.6997539300463558 2 34 2.2061330951389806 2 35 2.273553913073748 2 36 5.658079983718068 2 37 5.957513221115274 2 38 6.907992740122598 2 39 8.95279406132597 2 40 11.823994842521799 2 41 8.264414316111491 2 42 3.4419620919264773 2 43 -4.227533132074763 2 44 -9.595795136093018 2 45 0.1721840879847143 2 46 -10.464419821900943 2 47 -8.578062064838047 2 48 -5.798534910023246 2 49 -11.756032138743048 2 50 -6.62310544559787 2 51 -5.580822507791098 2 52 -5.3985413997222125 2 53 -5.339220921726252 2 54 -13.045962860635216 2 55 -5.377530048856038 2 56 -3.4642908839509943 3 1 1.5164546151330285 3 2 2.815584081036299 3 3 2.520335376816476 3 4 3.3124394987551113 3 5 2.866741782090436 3 6 3.3868277288849598 3 7 3.0835146159030558 3 8 3.013015851435881 3 9 3.2159354744702213 3 10 3.282487350952934 3 11 2.0159739132068353 3 12 2.421422150005636 3 13 -4.530806675115805 3 14 -4.125968547702339 3 15 -0.4072658790224466 3 16 3.8638795652542406 3 17 3.0402498276378545 3 18 3.006378710707555 3 19 1.9438597363870507 3 20 1.9815577854085937 3 21 4.271332366429569 3 22 4.39062838712384 3 23 3.8515308357586377 3 24 4.786582802092134 3 25 3.6278270804118407 3 26 3.0733881636028073 3 27 2.46840897188671 3 28 3.1090267142525434 3 29 2.7187287104597644 3 30 2.519179134896362 3 31 3.3888199303027093 3 32 2.6800035272776874 3 33 3.8399338400913336 3 34 3.780345988692204 3 35 3.113134122968802 3 36 5.679069488965439 3 37 6.258112921265198 3 38 6.752070701142085 3 39 8.781879518597332 3 40 11.998657511187467 3 41 11.066513266636228 3 42 9.055155495224827 3 43 1.2694683671756124 3 44 5.571371280698585 3 45 9.121959200428492 3 46 1.1120419472145002 3 47 -3.12228995089502 3 48 1.2262027211611617 3 49 -9.512403952836003 3 50 0.5807926053766437 3 51 -0.21275654077460615 3 52 -0.24486823630392252 3 53 -1.2747531556093108 3 54 -6.924523580275395 3 55 -0.7028673801903693 3 56 0.7298620395872355 4 1 2.0410447790674553 4 2 3.3215196378480556 4 3 2.768215535097987 4 4 3.751953348387673 4 5 3.0922929409372415 4 6 3.550196863989541 4 7 3.2994558256260618 4 8 3.6973007581628465 4 9 3.591571426985773 4 10 3.8449520202576295 4 11 5.2754425395494025 4 12 5.362349227450519 4 13 7.289148101594204 4 14 4.470130943535647 4 15 7.933887314872411 4 16 7.555513256605284 4 17 7.404579957654814 4 18 6.366469157287543 4 19 5.909659623328832 4 20 6.074265529897001 4 21 4.0398456507710065 4 22 4.553997522228421 4 23 4.3399423676975815 4 24 4.7775381440932705 4 25 4.3084375948267635 4 26 3.9040834716864694 4 27 3.7315519905413126 4 28 2.6618814344334325 4 29 3.9906337415507735 4 30 3.591536398887321 4 31 4.045688217470598 4 32 5.142129021907419 4 33 3.3376726755916124 4 34 5.052533665345685 4 35 4.164858260899834 4 36 6.64204292028257 4 37 7.818881717195101 4 38 7.903003431498217 4 39 10.093920219558324 4 40 13.01109391593591 4 41 6.08658110157122 4 42 10.039609989289268 4 43 9.113165371132649 4 44 12.836492818290445 4 45 10.714949590479371 4 46 9.724817526637903 4 47 10.627568726323748 4 48 9.112013913925434 4 49 8.832706278933358 4 50 10.30775699203577 4 51 8.303354256285878 4 52 8.484922605979854 4 53 8.860351423248805 4 54 1.3360756283170296 4 55 7.03088049996791 4 56 5.901710541565755 5 1 2.43645461513303 5 2 3.4162737362087086 5 3 2.607231928540614 5 4 3.8758877746171834 5 5 3.312948678642158 5 6 3.562000142678066 5 7 3.8076525469375397 5 8 3.9068089548841556 5 9 3.673866508952983 5 10 3.7059356268150054 5 11 5.631835982172355 5 12 7.966939391384944 5 13 8.30160711798764 5 14 7.074721107470072 5 15 8.076182396839624 5 16 8.500431289392171 5 17 7.981629137982683 5 18 7.40155112450066 5 19 6.961790770869815 5 20 5.773281923339628 5 21 4.324435814705431 5 22 5.02580080091694 5 23 4.6625653185172595 5 24 4.689341422781794 5 25 4.400240873515287 5 26 4.057526094637289 5 27 3.9656503511970485 5 28 4.050406024597372 5 29 4.460797675977005 5 30 3.8971101693791255 5 31 5.018475102716501 5 32 5.776555251415619 5 33 4.320623495263746 5 34 5.174828747312894 5 35 5.0448582608998365 5 36 6.552862592413717 5 37 7.2470784385065805 5 38 7.561036218383467 5 39 9.066707104804227 5 40 9.444864407739189 5 41 3.8078925769810574 5 42 11.092396874535176 5 43 14.924640780968716 5 44 13.995509211733072 5 45 11.506097131462973 5 46 11.405145395490361 5 47 12.17150315255326 5 48 11.571030307368058 5 49 12.784837426474343 5 50 11.609068467445606 5 51 11.054829666121943 5 52 11.327545556799524 5 53 10.539039947838965 5 54 10.700993661103915 5 55 10.703339516361353 5 56 8.557448246483787 6 1 1.5549161535945686 6 2 2.8978121977471716 6 3 1.8041550054636915 6 4 2.7043493130787226 6 5 2.7991025247960053 6 6 3.1958462965242163 6 7 2.749960239245233 6 8 3.157578185653392 6 9 2.9500203551068296 6 10 3.1743971652765453 6 11 4.137989828326198 6 12 7.233093237538792 6 13 5.73776096414149 6 14 6.3024134151623805 6 15 7.2154131660703875 6 16 7.875815904776783 6 17 7.262398368751914 6 18 6.584628047577578 6 19 5.427944617023659 6 20 6.187128077185779 6 21 3.87212812239774 6 22 4.4111854163015565 6 23 3.859488395440337 6 24 4.307802961243333 6 25 4.011779335053745 6 26 2.278295325406525 6 27 2.5241118896585917 6 28 3.1196367938281355 6 29 3.3884899836693094 6 30 3.2917255539945103 6 31 3.9630904873318897 6 32 4.1919398668002295 6 33 3.5952388798791333 6 34 3.9494441319282814 6 35 2.7225505685921405 6 36 3.28517028472141 6 37 3.019386130814272 6 38 4.263343910691159 6 39 5.626707104804229 6 40 1.9694797923545728 6 41 0.8271233462118275 6 42 6.591627643765943 6 43 11.097717704045639 6 44 9.468586134809993 6 45 7.288404823770669 6 46 8.674376164721131 6 47 9.406887767937874 6 48 9.071030307368058 6 49 12.543298964935882 6 50 9.490606928984068 6 51 9.499445050737329 6 52 8.677545556799526 6 53 8.408270717069737 6 54 8.887147507257762 6 55 8.421801054822893 6 56 7.018986708022247 7 1 2.0255455242239364 7 2 2.9453646452996196 7 3 2.299959201267882 7 4 3.5704332291626386 7 5 2.3074941331876104 7 6 3.351091051768975 7 7 3.116743456028452 7 8 3.417718045793251 7 9 3.326593781680259 7 10 3.1859356268150023 7 11 4.837290527626898 7 12 7.505121209566763 7 13 7.94160711798764 7 14 8.231084743833708 7 15 7.48709148774871 7 16 6.714976743937623 7 17 6.672538228891771 7 18 6.134278397227934 7 19 5.636336225415267 7 20 4.733281923339629 7 21 3.782617632887252 7 22 4.482164437280581 7 23 3.6916562276081635 7 24 4.632977786418159 7 25 3.4729681462425575 7 26 3.842980640091838 7 27 2.6492867148334156 7 28 3.4594969336882784 7 29 3.908070403249731 7 30 3.402564714833666 7 31 4.3148387390801375 7 32 5.676555251415618 7 33 3.560623495263748 7 34 4.023919656403805 7 35 3.3648582608998368 7 36 2.5564989560500777 7 37 3.2543511657793083 7 38 3.6210362183834626 7 39 5.053979832076955 7 40 -1.3242265013517205 7 41 -8.373925604837124 7 42 4.981487783626083 7 43 10.49736805369599 7 44 8.259145575369434 7 45 6.446097131462977 7 46 9.432418122763089 7 47 10.518775879825984 7 48 9.825575761913512 7 49 12.74120106283798 7 50 10.283613921991062 7 51 12.48755693884922 7 52 12.182091011344983 7 53 12.266312675111692 7 54 13.677357297467548 7 55 13.13788497090681 7 56 12.08472097375651 8 1 2.0674750232962964 8 2 2.9872941443719796 8 3 2.4517217244589773 8 4 3.201602060331467 8 5 3.024377250070728 8 6 2.9399593263515342 8 7 3.3994892816314177 8 8 3.7635436487617113 8 9 3.037539978340739 8 10 3.487976443141534 8 11 5.230611492376433 8 12 7.723674085262498 8 13 7.019158138395806 8 14 7.096761923796603 8 15 6.420672192757987 8 16 7.607370064902373 8 17 6.230608729819412 8 18 6.804408267357804 8 19 4.8879132198494055 8 20 5.941445188645748 8 21 3.7072929575625793 8 22 4.331515086631228 8 23 3.5376673593335823 8 24 4.2693414227817925 8 25 3.9022816898418142 8 26 2.440383237494437 8 27 2.281568718543987 8 28 2.0034672490871657 8 29 3.1318180841402707 8 30 2.4734366999913675 8 31 3.9560261231246656 8 32 4.505126679987047 8 33 3.7769500258759905 8 34 3.8658491554761625 8 35 4.1852664241651425 8 36 2.7357197352708624 8 37 2.674017214016782 8 38 3.7189954020569367 8 39 4.555278533375656 8 40 -2.660033551444485 8 41 -8.78394415771282 8 42 7.133621364331091 8 43 11.057293842193204 8 44 9.581223497447354 8 45 7.846097131462976 8 46 10.945961722020972 8 47 11.852727642349176 8 48 12.203683368592547 8 49 14.259939467290668 8 50 11.457639896017039 8 51 12.716870482448474 8 52 13.192851679248502 8 53 13.44516239681856 8 54 12.918544681512078 8 55 13.087421149014418 8 56 12.088060491381746 9 1 2.1956998981518936 9 2 2.9268397739445575 9 3 2.7366658908047654 9 4 3.557397208579445 9 5 3.0378543390195176 9 6 3.136339765319569 9 7 3.0340676412771614 9 8 3.2475636718652936 9 9 2.9293382070661877 9 10 3.1595205324753834 9 11 4.499383151983672 9 12 7.471845051762305 9 13 6.877456174591419 9 14 6.428683371621016 9 15 7.649012585518864 9 16 7.4302426101468875 9 17 6.932572534209097 9 18 6.793626596198774 9 19 5.110092657662268 9 20 5.891772489377363 9 21 2.960662229799773 9 22 4.140140423558449 9 23 3.5750181487059365 9 24 4.778775385045947 9 25 3.4606182320058494 9 26 3.542809113505214 9 27 2.674329596480071 9 28 2.331538100069068 9 29 3.6562693740902077 9 30 2.60050639579422 9 31 3.4558335932825415 9 32 3.623725062736373 9 33 3.26251028771658 9 34 3.8242627095770487 9 35 3.995046940145116 9 36 3.234372026375979 9 37 2.9874557969971427 9 38 3.7455645202702605 9 39 4.070103331219322 9 40 -3.622682762072131 9 41 -4.550597989056678 9 42 5.015793100950265 9 43 9.952942667761167 9 44 9.29550921173307 9 45 6.600814112595053 9 46 11.09382464077338 9 47 11.112257869534393 9 48 10.891785024349193 9 49 12.750120445342265 9 50 9.06680431650221 9 51 9.860112684989868 9 52 10.606790839818391 9 53 9.17677579689557 9 54 8.437220076198255 9 55 9.3361697050406 9 56 10.010278435163034 10 1 1.9246178804391505 10 2 2.6199472055964677 10 3 2.696619683642652 10 4 3.487316346045752 10 5 2.6366221480299146 10 6 2.858326673290307 10 7 2.9913260163252957 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5.456110696571098 114 38 5.756520089351209 114 39 8.255739362868749 114 40 11.262283762577901 114 41 10.194989351174605 114 42 7.136913003567432 114 43 -0.22052050935386447 114 44 0.23099308270080954 114 45 6.200935841140396 114 46 -0.9335642819289944 114 47 -10.240754911962872 114 48 -1.0096148539222654 114 49 -12.914517412235334 114 50 -1.1967379841672958 114 51 -1.4819445274264424 114 52 -1.040196378684346 114 53 -2.0222503747416773 114 54 -2.217070855025117 114 55 -1.665047580412839 114 56 2.299383730354755 115 1 0.8497879484663642 115 2 1.621458921393895 115 3 0.8938985952072755 115 4 1.9158877746171825 115 5 1.5270227527162348 115 6 2.3012594019373225 115 7 2.0669118061967993 115 8 2.1860682141434182 115 9 2.0760887311752043 115 10 2.0526022934816694 115 11 -0.9303862400498701 115 12 -2.890838386392833 115 13 0.17864415502468134 115 14 -5.449723336974369 115 15 -3.0630768624196385 115 16 -6.25808722912635 115 17 -9.366519010165469 115 18 -0.7088192458697122 115 19 -2.0108018217227794 115 20 -2.7259773359196338 115 21 1.3051765554461738 115 22 1.6013563564725004 115 23 0.8196023555542951 115 24 1.7137858672262354 115 25 -0.38494431166989784 115 26 0.7419705390817342 115 27 0.9300947956414944 115 28 0.23929491348625476 115 29 1.691908787088117 115 30 0.7311842434531997 115 31 0.11551213975354102 115 32 -6.108629933769571 115 33 -0.8912283565881083 115 34 -0.20368977120562448 115 35 -0.8721787761372042 115 36 3.259529259080381 115 37 3.1789302903584336 115 38 4.635851033198282 115 39 6.089670067767191 115 40 8.21449403736882 115 41 7.474559243647725 115 42 2.442767244905543 115 43 0.2764926328205668 115 44 -2.037824121600263 115 45 2.697948983314827 115 46 -2.5392990489540814 115 47 -6.801089440039336 115 48 -3.9474882111504606 115 49 -6.791458869821954 115 50 -0.11907968070254071 115 51 1.2444592957515752 115 52 2.1868048160587854 115 53 -0.26836745956844155 115 54 0.8928455129557662 115 55 0.21667284969468525 115 56 4.7796704687060085 116 1 1.2497879484663628 116 2 2.3696070695420417 116 3 1.927231928540614 116 4 2.315887774617181 116 5 2.1529486786421614 116 6 2.5753334760113944 116 7 2.0743192136042055 116 8 1.9934756215508287 116 9 1.9871998422863157 116 10 1.519268960148338 116 11 -1.7414973511609837 116 12 -2.4797272752817214 116 13 -7.79172621534569 116 14 -13.205278892529925 116 15 -7.803817603160379 116 16 -8.172902043941164 116 17 -9.985037528683986 116 18 -4.638448875499343 116 19 -4.451542562463523 116 20 -3.8333847433270414 116 21 1.0977691480387648 116 22 1.5124674675836118 116 23 1.3825653185172584 116 24 0.2693414227817925 116 25 0.3669075401819555 116 26 0.8975260946372927 116 27 1.1856503511970544 116 28 1.5504060245973719 116 29 1.6141310093103343 116 30 1.0237768360457906 116 31 -0.22152489728349423 116 32 -10.223444748584384 116 33 0.027290161930416446 116 34 0.914828747312896 116 35 0.19819159423316535 116 36 3.8595292590803822 116 37 4.227078438506577 116 38 4.454369551716798 116 39 7.060040438137559 116 40 10.218197741072522 116 41 4.307892576981057 116 42 0.6057302078685076 116 43 -10.108692552364618 116 44 -1.5711574549335978 116 45 -1.8539028685370234 116 46 -7.294854604509638 116 47 -10.875163514113408 116 48 -9.828969692631942 116 49 -8.22849590685899 116 50 -7.637598199221058 116 51 -5.118503667211389 116 52 -4.372454443200475 116 53 -8.2276267188277 116 54 -6.525673005562751 116 55 -5.983327150305314 116 56 -4.742551753516214 117 1 -0.04604538486697152 117 2 1.1862737362087117 117 3 0.41473192854061125 117 4 1.2908877746171825 117 5 0.5779486786421586 117 6 1.354500142678063 117 7 0.43265254693753974 117 8 0.3018089548841587 117 9 0.04136650895298288 117 10 0.5109356268150052 117 11 -9.270664017827649 117 12 -13.113060608615054 117 13 -4.920892882012357 117 14 -8.567778892529926 117 15 -6.553817603160379 117 16 -11.67706871060783 117 17 -6.65587086201732 117 18 -3.338448875499342 117 19 -3.0432092291301878 117 20 -2.8417180766603742 117 21 1.5019358147054334 117 22 0.7333008009169433 117 23 0.34506531851725697 117 24 -3.5431585772182075 117 25 0.4877408735152855 117 26 2.3600260946372913 117 27 0.4231503511970516 117 28 1.787906024597369 117 29 1.7682976759770028 117 30 -1.0303898306208765 117 31 -1.046524897283497 117 32 -18.423444748584384 117 33 -2.2268765047362535 117 34 -1.7476712526871054 117 35 -2.772641739100166 117 36 -0.15713740758628347 117 37 0.7020784385065788 117 38 0.3210362183834654 117 39 2.501707104804229 117 40 4.14736440773919 117 41 1.7453925769810574 117 42 -0.11510312546482737 117 43 -10.512859219031284 117 44 -9.76699078826693 117 45 0.34609713146297594 117 46 -2.1573546045096386 117 47 -11.833496847446742 117 48 -6.8039696926319415 117 49 -11.370162573525658 117 50 -3.158431532554392 117 51 -2.9476703338780545 117 52 -2.197454443200474 117 53 -4.610960052161033 117 54 -9.084006338896085 117 55 -5.179160483638647 117 56 -1.755051753516213 118 1 1.0549161535945686 118 2 1.9747352746702518 118 3 1.7656934670021514 118 4 2.4158877746171825 118 5 2.029871755565239 118 6 2.080461681139603 118 7 2.2307294700144595 118 8 2.3498858779610785 118 9 1.930789585876063 118 10 2.270551011430392 118 11 -7.131240940904572 118 12 -10.036137531691978 118 13 -10.28146980508928 118 14 -1.5437404309914662 118 15 -15.611509910852687 118 16 -10.124184095223214 118 17 -12.25683240047886 118 18 -4.376910413960882 118 19 -6.302824613745571 118 20 -5.274410384352681 118 21 -0.05094880067917984 118 22 -0.3388145836984435 118 23 -1.2943577584058197 118 24 -2.538350884910514 118 25 -0.3151437418693277 118 26 -3.2024739053627087 118 27 -1.5528111872644885 118 28 -2.8034401292487843 118 29 -2.149971554792227 118 30 -1.8813513690824166 118 31 -4.383063358821957 118 32 -11.423444748584384 118 33 -3.423991889351637 118 34 -3.877478944994799 118 35 -4.219757123715549 118 36 0.5928625924137165 118 37 0.711693823121962 118 38 0.5133439106911588 118 39 3.2036301817273056 118 40 6.777172100046883 118 41 5.615584884673364 118 42 0.6108584129967127 118 43 -0.19074383441590115 118 44 1.1801245963484526 118 45 -2.5385182531524073 118 46 -7.402546912201947 118 47 -10.24695838590828 118 48 -8.544354308016556 118 49 -7.341316419679504 118 50 -5.1632392248620835 118 51 -4.308247256954978 118 52 -5.341685212431244 118 53 -7.476344667545648 118 54 -7.382083261973008 118 55 -6.847429714407877 118 56 -4.096397907362366 119 1 0.5640736627520795 119 2 0.7696070695420474 119 3 1.2748509761596623 119 4 1.3444592031886131 119 5 1.6910439167373994 119 6 1.8039049045828222 119 7 1.8076525469375397 119 8 1.1172851453603485 119 9 1.5205331756196472 119 10 0.9097451506245307 119 11 -11.089116398780028 119 12 -9.303536799091244 119 13 -1.7631547867742619 119 14 -3.6243265115775465 119 15 -4.375246174588952 119 16 -9.977663948703068 119 17 -7.980275623922083 119 18 -2.528925065975532 119 19 -2.465828276749235 119 20 -2.133384743327042 119 21 -1.7688975186278988 119 22 -1.7161039609878195 119 23 -0.6936251576732175 119 24 -6.968753815313445 119 25 -2.580711507437094 119 26 -0.7024739053627087 119 27 1.7237455892922924 119 28 0.7313584055497486 119 29 0.15222624740557222 119 30 -0.6286041163351612 119 31 -0.11200108775968687 119 32 -7.732968558108194 119 33 0.5320520666923159 119 34 -0.11374268125853604 119 35 -0.2160941200525457 119 36 2.973814973366096 119 37 3.279459390887528 119 38 4.7257981231453705 119 39 4.364802342899466 119 40 10.061054883929664 119 41 3.1412259103143896 119 42 -0.5466507445124478 119 43 -4.4801211237931895 119 44 -3.7044907882669307 119 45 -2.725331439965597 119 46 -6.713902223557257 119 47 -8.208496847446742 119 48 -10.571826835489084 119 49 -8.304686383049468 119 50 -7.209026770649629 119 51 -8.147075095782817 119 52 -9.072454443200474 119 53 -13.813341004541986 119 54 -13.768530148419895 119 55 -15.497612864591028 119 56 -13.68064699161145 120 1 0.7564546151330305 120 2 2.1962737362087097 120 3 1.147231928540613 120 4 2.275887774617182 120 5 1.3729486786421603 120 6 2.002000142678064 120 7 1.887652546937538 120 8 2.1268089548841616 120 9 2.0138665089529795 120 10 0.9459356268150074 120 11 -6.46816401782765 120 12 -1.5530606086150556 120 13 -6.498392882012357 120 14 -6.085278892529924 120 15 -5.963817603160379 120 16 -3.179568710607832 120 17 -2.8183708620173213 120 18 -0.5384488754993413 120 19 -1.2182092291301885 120 20 -1.7467180766603754 120 21 1.3244358147054314 120 22 2.725800800916943 120 23 1.1425653185172564 120 24 -2.290658577218206 120 25 0.6002408735152827 120 26 1.8975260946372927 120 27 2.1656503511970513 120 28 2.0304060245973687 120 29 2.5007976759770045 120 30 1.8571101693791263 120 31 1.0384751027165038 120 32 -8.483444748584382 120 33 -1.4393765047362521 120 34 -5.325171252687106 120 35 1.2448582608998322 120 36 2.1128625924137197 120 37 1.6870784385065782 120 38 3.1010362183834665 120 39 2.966707104804229 120 40 0.10486440773918915 120 41 0.207892576981056 120 42 -5.367603125464829 120 43 -2.255359219031284 120 44 -6.104490788266931 120 45 -10.033902868537025 120 46 -14.294854604509638 120 47 -13.288496847446742 120 48 -11.908969692631942 120 49 -13.895162573525658 120 50 -11.810931532554392 120 51 -12.425170333878054 120 52 -12.432454443200474 120 53 -12.380960052161033 120 54 -14.199006338896085 120 55 -12.796660483638647 120 56 -10.322551753516214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 773.0 5 86.0 6 44.0 7 43.0 8 88.0 9 97.0 10 57.0 11 62.0 12 45.0 13 86.0 14 110.0 15 151.0 16 186.0 17 224.0 18 293.0 19 362.0 20 403.0 21 553.0 22 701.0 23 850.0 24 966.0 25 979.0 26 1067.0 27 1106.0 28 1276.0 29 1307.0 30 1478.0 31 1690.0 32 1984.0 33 2513.0 34 2825.0 35 3458.0 36 3753.0 37 3244.0 38 1551.0 39 402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.1727228334243 0.7238675207537414 0.06319478355786631 0.04021486226409674 8 98.96877603194208 0.8904719501335707 0.08617470485163589 0.05457731307270272 9 95.11592495762346 3.513661045192059 0.6751515499755796 0.6952624472088947 10 40.8180851980582 33.32088587596587 15.247177778416109 10.613851147559819 11 35.927266459841434 27.786395495806044 18.07997242330231 18.206365621050214 12 33.26056358256973 19.133656967224887 20.090196191078046 27.515583259127336 13 30.057737052250594 14.514692787177205 23.313130152529226 32.11444000804297 14 21.09013590782404 20.742464730052006 24.460535011349595 33.70686435077436 15 23.322977793119318 17.64181337015303 29.844091589000115 29.191117247727533 16 31.217493750179592 17.157552943880923 25.691790465791215 25.93316284014827 17 31.853575841163117 21.50676665804672 20.54707927477516 26.092578226015 18 27.21762610594048 30.07870849132483 19.504768470642308 23.19889693209238 19 23.9017382559977 26.44160321792846 18.327826461715272 31.32883206435857 20 25.522988505747122 22.126436781609197 26.67816091954023 25.67241379310345 21 25.32605573111175 20.287273771904626 27.397299626544097 26.989370870439526 22 25.58673982361897 26.974232282899084 24.130303639654134 23.308724253827815 23 27.156521988912186 28.779478930284665 23.620486599833395 20.44351248096975 24 23.73251371614052 30.367965989716485 22.770229511963922 23.129290782179073 25 26.634493852694476 24.210042514075607 26.597150407905318 22.5583132253246 26 27.94947053406998 22.03326427255985 29.01991252302026 20.99735267034991 27 23.686781609195403 22.873563218390807 28.96264367816092 24.477011494252874 28 20.303823690191905 27.67500071927957 30.87147912650689 21.149696464021638 29 21.39635109898003 19.844849877891107 37.90834650193938 20.85045252118948 30 18.885057471264368 19.04597701149425 42.60632183908046 19.46264367816092 31 24.98416354736539 18.327094730780306 38.070832133602075 18.617909588252232 32 23.22121046735415 20.01838393703502 36.98905581248384 19.771349783126993 33 20.546171333199045 17.740496676354635 40.00748179908492 21.7058501913614 34 24.412119818317716 17.259817167826135 41.476456045535556 16.85160696832059 35 25.29601103575124 18.450396597310036 40.77767559489596 15.475916772042764 36 23.947633434038266 18.618903754855417 40.696302690260396 16.73716012084592 37 19.218475537766004 18.691508048492526 45.05140092723241 17.038615486509055 38 18.862008457267784 25.86945890745908 41.52403417426574 13.744498461007392 39 17.87556554566151 16.645053456672716 36.511916083109995 28.967464914555773 40 14.435968562182156 13.96208969024503 28.819926028663893 42.78201571890892 41 14.721811915312655 15.243143047470095 30.97575810235468 39.05928693486257 42 15.911249244582578 24.927336038447148 40.97384097384097 18.1875737431293 43 10.669395756457565 12.569188191881919 31.567112546125458 45.19430350553505 44 11.687112137393562 16.377543657093376 29.897532111415785 42.03781209409727 45 22.502804222151916 19.46849204751359 41.76133912393224 16.267364606402253 46 18.464324728338987 18.86678548841488 46.54171218306215 16.12717760018398 47 15.790834650193938 17.69860652205143 43.697744576928606 22.81281425082603 48 17.500502888014026 19.744820253455558 42.19948849104859 20.555188367481826 49 11.703170028818445 14.097982708933717 32.93948126801153 41.25936599423631 50 20.679927459051786 20.533118397190478 43.28852298569331 15.498431158064424 51 18.55705761969273 25.38840688323293 40.235782434496876 15.818753062577464 52 21.178573487862536 22.65467335524419 39.68459897364931 16.48215418324396 53 16.595193553029215 22.68527845733199 44.97337746438336 15.74615052525543 54 15.665526066078156 18.966558357535153 35.92604307444576 29.441872501940935 55 22.06271576524741 20.546605293440738 40.22151898734177 17.16915995397008 56 23.588326713866344 21.815460772463016 37.788522419846885 16.807690093823748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 3.5 9 4.0 10 22.0 11 40.0 12 50.0 13 67.0 14 74.0 15 106.5 16 139.0 17 185.0 18 231.0 19 284.5 20 338.0 21 401.5 22 579.0 23 693.0 24 835.5 25 978.0 26 1130.0 27 1282.0 28 1411.0 29 1582.5 30 1625.0 31 1571.0 32 1517.0 33 1364.5 34 1212.0 35 1028.0 36 844.0 37 772.5 38 675.5 39 650.0 40 647.0 41 644.0 42 621.5 43 599.0 44 640.5 45 682.0 46 786.0 47 1057.5 48 1225.0 49 1381.5 50 1538.0 51 1452.5 52 1367.0 53 1407.5 54 1525.5 55 1603.0 56 1645.0 57 1687.0 58 1793.5 59 1900.0 60 1865.0 61 1830.0 62 1741.0 63 1544.5 64 1437.0 65 1285.0 66 1133.0 67 996.5 68 860.0 69 792.5 70 725.0 71 605.0 72 390.5 73 296.0 74 251.5 75 207.0 76 158.5 77 110.0 78 84.5 79 42.5 80 26.0 81 18.5 82 11.0 83 7.0 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.017234940970327177 10 0.0 11 0.0028724901617211964 12 0.0 13 0.0 14 0.028724901617211963 15 0.1407520179243386 16 0.034469881940654354 17 0.028724901617211963 18 0.0028724901617211964 19 0.02297992129376957 20 0.03734237210237555 21 0.008617470485163588 22 0.005744980323442393 23 0.0 24 0.0 25 0.0028724901617211964 26 0.17522189986499298 27 0.03734237210237555 28 0.16085944905638697 29 0.02297992129376957 30 0.03734237210237555 31 0.2384166834228593 32 0.0 33 0.17809439002671415 34 0.0775572343664723 35 0.04883233274926034 36 0.1666044293798294 37 0.24703415390802289 38 0.1436245080860598 39 0.32171889811277393 40 0.5888604831528452 41 0.821532186252262 42 0.18383937035015657 43 0.35906127021514955 44 0.4825783471691609 45 0.12638956711573263 46 0.0775572343664723 47 0.02297992129376957 48 0.04021486226409674 49 0.3245913882744952 50 0.21256427196736852 51 0.34469881940654357 52 0.3648062505385919 53 0.19532933099704136 54 0.10340964582196306 55 0.1522419785712234 56 0.19245684083532014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 34813.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.86335564300693 #Duplication Level Percentage of deduplicated Percentage of total 1 94.40457719162141 83.89107517306753 2 3.866046030514611 6.870996466837101 3 0.7790276700284459 2.076810386924425 4 0.32648047582104994 1.1604860253353633 5 0.15192655805534006 0.6750351880044811 6 0.09374191880010345 0.4998132881394881 7 0.07111455908973364 0.4423634849050642 8 0.06464959917248513 0.4595984258753914 9 0.04848719937936385 0.38778617183236147 >10 0.1777863977243341 2.7030132421796456 >50 0.01616239979312128 0.833022146899147 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCGG 65 0.18671186051187774 No Hit ATACAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGG 64 0.18383937035015657 No Hit ATACAGGGGTGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCGG 55 0.1579869588946658 No Hit ATACAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 53 0.1522419785712234 No Hit ATACAGGGGTGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCGG 53 0.1522419785712234 No Hit ATACAGGGGTGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCGG 49 0.1407520179243386 No Hit ATACAGGGGTGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGG 47 0.13500703760089622 No Hit ATACAGGGGTGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCGG 44 0.12638956711573263 No Hit ATACAGGGGTGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCGG 40 0.11489960646884785 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.002872490161721196 0.0 0.0 0.0 0.0 12 0.005744980323442392 0.0 0.0 0.0 0.0 13 0.005744980323442392 0.0 0.0 0.0 0.0 14 0.008617470485163588 0.0 0.0 0.0 0.0 15 0.011489960646884784 0.0 0.0 0.0 0.0 16 0.011489960646884784 0.0 0.0 0.0 0.0 17 0.011489960646884784 0.0 0.0 0.0 0.0 18 0.011489960646884784 0.0 0.0 0.0 0.0 19 0.017234940970327177 0.0 0.0 0.0 0.0 20 0.022979921293769568 0.0 0.0 0.0 0.0 21 0.025852411455490765 0.0 0.0 0.0 0.0 22 0.025852411455490765 0.0 0.0 0.0 0.0 23 0.025852411455490765 0.0 0.0 0.0 0.0 24 0.03159739177893316 0.0 0.0 0.0 0.0 25 0.03159739177893316 0.0 0.0 0.0 0.0 26 0.034469881940654354 0.0 0.0 0.0 0.0 27 0.03734237210237555 0.0 0.0 0.0 0.0 28 0.04308735242581794 0.0 0.0 0.0 0.0 29 0.045959842587539136 0.0 0.0 0.0 0.0 30 0.05170482291098153 0.0 0.0 0.0 0.0 31 0.05170482291098153 0.0 0.0 0.0 0.0 32 0.05170482291098153 0.0 0.0 0.0 0.0 33 0.05170482291098153 0.0 0.0 0.0 0.0 34 0.05170482291098153 0.0 0.0 0.0 0.0 35 0.05170482291098153 0.0 0.0 0.0 0.0 36 0.05170482291098153 0.0 0.0 0.0 0.0 37 0.05170482291098153 0.0 0.0 0.0 0.0 38 0.05170482291098153 0.0 0.0 0.0 0.0 39 0.05170482291098153 0.0 0.0 0.0 0.0 40 0.05170482291098153 0.0 0.0 0.0 0.0 41 0.05170482291098153 0.0 0.0 0.0 0.0 42 0.05170482291098153 0.0 0.0 0.0 0.0 43 0.05170482291098153 0.0 0.0 0.0 0.0 44 0.057449803234423925 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATTTA 20 3.9409826E-4 50.46939 37 TATTTAT 20 3.9409826E-4 50.46939 38 ATTTATT 20 3.9409826E-4 50.46939 39 TTCATTT 15 0.008058688 50.469387 38 CAACCTG 15 0.008058688 50.469387 41 TCATTTT 15 0.008058688 50.469387 39 CAAACCA 15 0.008058688 50.469387 39 TTTTCGT 15 0.008105323 50.395924 36 TAGCAGC 15 0.008105323 50.395924 35 TTGATAT 15 0.008246433 50.17681 49 CATTTGA 15 0.008293873 50.1042 46 TGATATG 15 0.008293873 50.1042 50 ATTTGAT 15 0.008293873 50.1042 47 CGTTCCG 40 2.5083864E-9 50.104195 44 GTAGAGC 15 0.008341516 50.03179 33 TGGTAGA 15 0.008341516 50.03179 31 TGCGCAC 15 0.008341516 50.03179 45 GTTTTGC 15 0.008341516 50.03179 28 TGGCTCG 25 2.0377158E-5 50.03179 31 GGAGAAA 25 2.0552585E-5 49.9596 20 >>END_MODULE