FastQCFastQC Report
Fri 17 Jun 2016
SRR1526350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35704
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1060.29688550302487116No Hit
TAAGGGGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG730.2044588841586377No Hit
TAAGGGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG630.17645081783553665No Hit
TAAGGGGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG610.1708492045709164No Hit
TAAGGGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG580.1624467846739861No Hit
TAAGGGGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG560.1568451714093659No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC550.1540443647770558No Hit
TAAGGGGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG540.1512435581447457No Hit
TAAGGGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG530.14844275151243558No Hit
TAAGGGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG520.14564194488012547No Hit
TAAGGGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG500.14004033161550525No Hit
TAAGGGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG450.12603629845395475No Hit
TAAGGGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG440.12323549182164464No Hit
TAAGGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCCAGGCTCACAACCAAA430.12043468518933453No Hit
TAAGGGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG400.1120322652924042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCG150.00817808650.2853140
CACCACT150.00817808650.2853141
GTACCCA204.0425846E-450.21438637
GGTACCC204.070875E-450.14365836
CACACAA150.008316250.07313545
ACCGTTC150.008316250.07313545
ACTTCGG150.008316250.07313545
CCTTTTC150.008316250.07313543
CACTTCG150.008316250.07313544
CGACTCC301.0090116E-650.07313544
ACTCCTC355.0391463E-850.0731346
CTACACC150.00836262250.00280821
CCAATAG150.00836262250.00280827
CTTTAAT150.00836262250.00280832
AACGAGT150.00836262250.00280829
GTCGTGC150.00836262250.00280830
GCGGTGA150.00836262250.00280823
GCGGTCG150.00836262250.00280827
TCGCGCA150.00836262250.00280850
TCGTGCT150.00836262250.00280831