##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526350_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35704 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.86018373291508 39.0 38.0 39.0 35.0 40.0 2 36.590157965494065 39.0 38.0 39.0 33.0 40.0 3 36.47448465157966 39.0 38.0 39.0 32.0 40.0 4 37.235295765180375 39.0 38.0 39.0 33.0 40.0 5 37.29705355142281 39.0 38.0 39.0 33.0 40.0 6 37.19731682724625 39.0 38.0 39.0 33.0 40.0 7 37.333127940846964 39.0 38.0 39.0 35.0 40.0 8 37.20140600492942 38.0 38.0 39.0 34.0 40.0 9 36.271902307864664 38.0 35.0 39.0 31.0 40.0 10 35.58836544924938 38.0 35.0 39.0 29.0 40.0 11 34.13427066995295 36.0 31.0 39.0 26.0 40.0 12 32.60102509522743 36.0 30.0 39.0 18.0 40.0 13 32.136063186197624 35.0 29.0 39.0 16.0 40.0 14 31.536158413623124 36.0 28.0 39.0 14.0 40.0 15 32.19849316603182 36.0 29.0 38.0 17.0 40.0 16 32.027559937261934 36.0 29.0 38.0 17.0 40.0 17 32.05021846291732 35.0 29.0 38.0 18.0 40.0 18 32.22524086937038 35.0 29.0 38.0 19.0 40.0 19 32.96700649787139 36.0 30.0 38.0 23.0 40.0 20 33.18384494734484 36.0 30.0 38.0 23.0 40.0 21 34.70992045709164 38.0 33.0 39.0 26.0 40.0 22 34.26652475913063 38.0 33.0 39.0 24.0 40.0 23 34.5081223392337 38.0 33.0 39.0 26.0 40.0 24 34.201742101725294 38.0 33.0 39.0 24.0 40.0 25 34.36536522518485 38.0 33.0 39.0 26.0 40.0 26 34.635419000672194 38.0 34.0 39.0 27.0 40.0 27 34.636987452386286 38.0 34.0 39.0 27.0 40.0 28 34.422809769213536 38.0 33.0 39.0 26.0 40.0 29 34.37147098364329 38.0 33.0 39.0 26.0 40.0 30 34.01092314586601 38.0 33.0 39.0 24.0 40.0 31 33.7953450593771 38.0 33.0 39.0 23.0 40.0 32 32.72056352229442 37.0 32.0 39.0 17.0 40.0 33 33.82441743222048 38.0 33.0 39.0 24.0 40.0 34 33.55427963253417 38.0 33.0 39.0 23.0 40.0 35 33.57531369034282 37.0 33.0 39.0 23.0 40.0 36 31.724876764508178 35.0 30.0 38.0 18.0 39.0 37 31.456251400403318 35.0 30.0 38.0 16.0 39.0 38 30.4026439614609 34.0 29.0 38.0 10.0 39.0 39 28.672445664351333 33.0 25.0 38.0 4.0 39.0 40 24.94532825453731 31.0 11.0 36.0 4.0 39.0 41 21.704542908357606 25.0 9.0 33.0 4.0 37.0 42 24.905024647098365 30.0 18.0 33.0 4.0 36.0 43 21.5531032937486 26.0 8.0 33.0 4.0 36.0 44 22.398694824109345 27.0 9.0 33.0 4.0 36.0 45 24.572400851445217 29.0 17.0 33.0 4.0 36.0 46 24.596599820748377 30.0 11.0 35.0 4.0 38.0 47 24.006749943983866 29.0 7.0 35.0 4.0 38.0 48 24.028848308312796 29.0 7.0 35.0 4.0 38.0 49 21.672305624019717 26.0 4.0 35.0 4.0 38.0 50 23.859175442527448 29.0 4.0 35.0 4.0 38.0 51 25.26462021062066 32.0 4.0 37.0 4.0 39.0 52 25.039295317051312 31.0 4.0 37.0 4.0 39.0 53 24.791591978489805 31.0 4.0 37.0 4.0 39.0 54 23.843434909253865 30.0 4.0 37.0 4.0 39.0 55 23.879033161550527 30.0 4.0 36.0 4.0 39.0 56 23.3466278288147 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.2418058076538827 1 2 2.299647186393635 1 3 2.5182178994153404 1 4 1.2620345349691107 1 5 1.1592428035081639 1 6 1.2982350586066218 1 7 0.91314419867156 1 8 1.309326983128848 1 9 1.729697525537759 1 10 1.2017300831588784 1 11 -6.375087328830588 1 12 -8.22084426864799 1 13 -21.36449186984915 1 14 -18.23009884982261 1 15 -12.6806934491334 1 16 -21.532361289051167 1 17 -21.587807371925624 1 18 -20.993891249285266 1 19 -6.29158304966224 1 20 -12.04060813103936 1 21 -8.087302596512615 1 22 -3.3882127816704966 1 23 -3.7668136767727347 1 24 -3.58622136515352 1 25 -3.7328583364554326 1 26 2.3414089644739917 1 27 2.56588998325676 1 28 2.0492016188114235 1 29 2.4604309286469572 1 30 2.672625810580776 1 31 1.7438472187885168 1 32 -11.76988574393279 1 33 0.9395460390460499 1 34 0.7813264887218452 1 35 1.540196576624389 1 36 3.4079618107515195 1 37 3.9750022104625806 1 38 4.4778481156828285 1 39 6.667039566209677 1 40 8.246615701371176 1 41 -3.6279576551463464 1 42 -7.298463373611183 1 43 -14.063641664008042 1 44 -15.30590121565217 1 45 -14.564490280637242 1 46 -17.196340509431494 1 47 -17.239331844650557 1 48 -16.012678420038263 1 49 -13.991313178016586 1 50 -15.747324168532002 1 51 -16.767759629165703 1 52 -16.393347432368927 1 53 -16.164614086348273 1 54 -17.19537055809559 1 55 -16.05754796712825 1 56 -15.496839131463581 2 1 0.3696644355125116 2 2 1.761705398451852 2 3 2.199091080288518 2 4 1.0354233083578848 2 5 1.205500599765955 2 6 0.9406467010182595 2 7 0.5373645728919385 2 8 0.9325078563097193 2 9 0.7364542822945168 2 10 1.1627487941775883 2 11 -5.342862796606056 2 12 -12.300885848689571 2 13 -14.223639478996759 2 14 -15.299225668949429 2 15 -8.79243981087976 2 16 -5.235063991753869 2 17 -2.9713832555015074 2 18 -11.146697902091919 2 19 -3.966219224298417 2 20 -5.306200646631872 2 21 1.9984562392462237 2 22 2.6138662204085037 2 23 1.4920220820630234 2 24 0.9007848718527143 2 25 1.5420896884925952 2 26 0.7790389021039275 2 27 0.741045908412687 2 28 0.061675631285432075 2 29 0.8871461053621346 2 30 1.7079688459238085 2 31 -1.3782941533528579 2 32 -8.38942836347541 2 33 -2.006399906899894 2 34 -1.8704406630453079 2 35 -1.2425477061198968 2 36 3.107546010335721 2 37 3.0779128133731852 2 38 3.6836693215040377 2 39 6.207580106750218 2 40 7.960233164988637 2 41 2.4775517003630085 2 42 -2.2392118143596242 2 43 -4.064681165047542 2 44 -11.090204749955705 2 45 -3.048897765044728 2 46 -11.873055686146667 2 47 -12.059498164816876 2 48 -9.7990609564208 2 49 -11.061479498182905 2 50 -9.072687993895826 2 51 -9.690836552242626 2 52 -9.643867182888675 2 53 -10.503491425225612 2 54 -14.984351847076876 2 55 -10.904741314321598 2 56 -9.718252852877303 3 1 0.6993347651828401 3 2 1.9704966072430565 3 3 2.3488163550137955 3 4 1.396687044621622 3 5 1.3112698305351884 3 6 1.0395477999193616 3 7 0.9247272102545736 3 8 0.7113540101558726 3 9 1.2900257108659474 3 10 1.7451663765951722 3 11 -0.3538518075950634 3 12 0.9147734919697719 3 13 -6.546441676798956 3 14 -9.50664325136701 3 15 -4.53831893175888 3 16 1.9077931511032773 3 17 0.37889146977321886 3 18 0.8478075924135773 3 19 -0.6434170264962198 3 20 -0.14823361366483923 3 21 2.5135661293561142 3 22 2.991613473155759 3 23 2.619769334810279 3 24 3.428257399325247 3 25 2.360771007173909 3 26 2.125192748257774 3 27 2.160001952368731 3 28 1.3281591477689503 3 29 3.263519731735755 3 30 2.111814999769962 3 31 2.5049476048889048 3 32 1.2026046035575604 3 33 2.529314378814391 3 34 2.6075813149766702 3 35 1.669540205968019 3 36 4.38913941692913 3 37 4.767473252933623 3 38 4.973504486339198 3 39 7.421865821035933 3 40 9.501441956197432 3 41 8.286617634428943 3 42 7.08908488893708 3 43 0.5053737800074032 3 44 3.507322722571768 3 45 6.918135201988239 3 46 -0.5172864553774374 3 47 -5.589717945036657 3 48 0.08143354907370437 3 49 -9.841699278402686 3 50 0.0330812368734037 3 51 -0.6784739148799872 3 52 -0.17133971036120244 3 53 -1.0227221944563816 3 54 -5.86621997894501 3 55 -2.1849610945413787 3 56 1.4713075866831353 4 1 1.008125973974046 4 2 2.496320783067233 4 3 2.6837064649039064 4 4 1.189269462204038 4 5 0.8631929074582629 4 6 1.3252620856336463 4 7 1.6719799575073182 4 8 1.0132770870789471 4 9 1.9633773592175956 4 10 2.5896718711006628 4 11 3.4955987418554813 4 12 3.306806459002736 4 13 2.718668213310931 4 14 4.4430820233582615 4 15 5.080637112197163 4 16 6.518782162092286 4 17 5.344001359883109 4 18 5.491763636369619 4 19 3.226088468009273 4 20 3.613030122598893 4 21 2.3330716238616063 4 22 2.690789297331584 4 23 2.130483620524565 4 24 3.573861794929641 4 25 2.738243534646436 4 26 1.3559619790270077 4 27 1.9833536007203776 4 28 0.9232140928238977 4 29 2.4371461053621317 4 30 1.846430384385343 4 31 2.9409366158779093 4 32 2.9182639442168963 4 33 1.9474462469462566 4 34 1.921867029262387 4 35 1.688221524649336 4 36 3.438315241104956 4 37 4.6394512749116465 4 38 3.9759770138117254 4 39 6.899887799057911 4 40 8.510233164988641 4 41 6.8660132388245465 4 42 8.24540357025576 4 43 6.98916498879861 4 44 11.929026019275064 4 45 8.520333004186043 4 46 7.8577135446225626 4 47 7.655886450567738 4 48 7.327862120502278 4 49 4.842366655663248 4 50 7.027312006104172 4 51 5.816855755449684 4 52 6.148440509419018 4 53 5.958047036312848 4 54 1.4694943067692776 4 55 6.379874070293788 4 56 4.843285608661159 5 1 0.61232177816985 5 2 2.089327776074228 5 3 1.9997903809878181 5 4 0.7794792524138288 5 5 0.6226334668988258 5 6 0.6888984492700132 5 7 0.9013505868779461 5 8 0.5908994647013301 5 9 0.8682724641126995 5 10 1.339322220751015 5 11 2.821472867729611 5 12 6.113799465995747 5 13 4.838947933590649 5 14 5.1605645408407845 5 15 5.774343405903455 5 16 6.146754190064314 5 17 4.882462898344649 5 18 5.476379020985007 5 19 4.775738817659629 5 20 3.8326105421793173 5 21 2.009994700784681 5 22 3.115964122506405 5 23 2.0759381659791103 5 24 4.089945711013556 5 25 2.262719059121963 5 26 2.125192748257774 5 27 0.8700668874336657 5 28 2.0924448620546627 5 29 2.2972859655019917 5 30 2.8799968179517776 5 31 2.296181371122671 5 32 3.5308513568043125 5 33 2.4117819112819276 5 34 2.4491397565351107 5 35 1.6085012449290517 5 36 3.3907627935525078 5 37 3.0772135126738824 5 38 3.4822707201054364 5 39 5.8984891976593055 5 40 4.387156241911718 5 41 0.7681111409224499 5 42 7.343305668157861 5 43 10.42972442935805 5 44 9.829725319974365 5 45 6.780472864325901 5 46 8.075895362804381 5 47 8.935606730288018 5 48 7.8187712114113666 5 49 7.583625396921988 5 50 8.003535782327948 5 51 7.104967643561569 5 52 7.416971977950485 5 53 8.163641441907256 5 54 6.760403397678367 5 55 7.672181762601479 5 56 7.190138755514305 6 1 1.1692595772129124 6 2 2.7681156548621075 6 3 2.439981768547625 6 4 1.6589050897344038 6 5 1.572234742815887 6 6 1.7463147172125915 6 7 1.543774829302194 6 8 1.353897869804996 6 9 2.1004893700138183 6 10 1.6104545971465498 6 11 3.5296068390214757 6 12 6.336765973172781 6 13 5.748357821947909 6 14 7.463594843871082 6 15 5.896831444180965 6 16 6.15090092052683 6 17 6.40176114395868 6 18 6.153572003441141 6 19 4.074941369493757 6 20 4.27375886753817 6 21 2.420521016574156 6 22 3.2575909167647836 6 23 3.0041678310508786 6 24 2.7196107827838887 6 25 1.508014114943336 6 26 1.5988769587840892 6 27 1.6152024536218619 6 28 0.608234335738878 6 29 1.1256751202069353 6 30 0.33549921029627683 6 31 1.583581690101937 6 32 2.7806121223545475 6 33 1.2041264088895787 6 34 1.585024923999228 6 35 1.4126479753915717 6 36 0.668274755274993 6 37 0.8370221251140748 6 38 2.2896072432314334 6 39 4.891790633066009 6 40 -0.6163525300181085 6 41 -0.6165778542928635 6 42 6.913959575653873 6 43 10.12222841659888 6 44 10.003250040867506 6 45 6.840440966399267 6 46 9.89280126392081 6 47 9.605144210351813 6 48 9.247160366116312 6 49 11.625092701547189 6 50 9.044046149154106 6 51 10.425222826974647 6 52 11.436429713197693 6 53 10.902716402034844 6 54 11.248441675190332 6 55 9.830076499443585 6 56 10.391733651846042 7 1 0.8305035963516687 7 2 2.7984186851651387 7 3 2.327063108260546 7 4 1.4885701615047395 7 5 1.4771789214442776 7 6 1.5070802674518262 7 7 1.7922596777870368 7 8 1.77271764651951 7 9 2.7046361004763355 7 10 2.3938676752964696 7 11 4.785109231365972 7 12 6.0410721932684694 7 13 6.438947933590651 7 14 6.869655449931688 7 15 6.53797976953982 7 16 6.4012996446097645 7 17 5.991553807435558 7 18 6.530924475530462 7 19 4.593920635841442 7 20 4.487155996724766 7 21 2.9009037916937714 7 22 4.479600486142772 7 23 3.0213927114336556 7 24 3.744491165559012 7 25 3.62635542275833 7 26 2.3979200209850475 7 27 2.797339614706388 7 28 2.2924448620546656 7 29 2.897285965501993 7 30 3.6072695452245043 7 31 2.605272280213576 7 32 3.2217604477134003 7 33 2.33905463855465 7 34 3.5218670292623884 7 35 2.6266830631108746 7 36 2.663490066279781 7 37 2.804486239946609 7 38 2.300452538287253 7 39 4.643943743113855 7 40 0.3144289691844442 7 41 -7.195525222713915 7 42 4.72512384997604 7 43 9.284269883903505 7 44 9.266088956338002 7 45 5.8895637734168105 7 46 8.0940771809862 7 47 8.044697639378928 7 48 9.164225756865914 7 49 9.98362539692199 7 50 7.185353964146131 7 51 7.923149461743389 7 52 7.235153796132302 7 53 7.872732350998163 7 54 7.8149488522238215 7 55 8.963090853510568 7 56 7.681047846423397 8 1 0.9236455421251932 8 2 2.8382910934585937 8 3 3.1241922948634198 8 4 2.0624138616642256 8 5 1.8529364972018527 8 6 1.483156822475749 8 7 1.7718450047407899 8 8 1.8100382206821877 8 9 1.381191124399777 8 10 1.3999282813570773 8 11 4.161185786389893 8 12 5.705187025804356 8 13 5.064147295632122 8 14 6.937279054397397 8 15 5.879287584531848 8 16 6.589497411754905 8 17 5.700006757993769 8 18 5.732519371862196 8 19 4.6714325975639355 8 20 4.080776411397814 8 21 3.122275402539067 8 22 3.7839067062384615 8 23 3.61820291877018 8 24 4.28101429155582 8 25 2.9466106061714044 8 26 2.2129120465033907 8 27 2.3695884185341427 8 28 2.4152518795985287 8 29 2.178306699154305 8 30 2.0372535962611877 8 31 1.9169150234352657 8 32 4.00868229779315 8 33 2.5550036018720377 8 34 1.8481828187360705 8 35 0.7635251683740307 8 36 -0.3843568236723769 8 37 -0.8471884012017128 8 38 0.1492563660384505 8 39 2.0496853699081115 8 40 -3.7917911265093345 8 41 -10.704297152538476 8 42 3.317468347583695 8 43 7.297667013090109 8 44 6.5471096899903145 8 45 4.752721668153654 8 46 8.068239860412035 8 47 9.149003859474622 8 48 7.966458611730346 8 49 11.30930322786298 8 50 7.833519833364633 8 51 9.705924581360613 8 52 9.787306906180152 8 53 10.078154998526077 8 54 10.037915359400856 8 55 9.935339657338321 8 56 7.356645932547799 9 1 1.2301470901128084 9 2 3.023017615646417 9 3 3.074656691148249 9 4 2.0489979689913653 9 5 2.0686227717116594 9 6 1.482124830731685 9 7 1.7006375744002327 9 8 1.944197147410776 9 9 2.662924870529814 9 10 2.4195361244943285 9 11 4.983683206410532 9 12 6.419325312698064 9 13 5.825757202752865 9 14 7.836143863478927 9 15 7.186821124263528 9 16 6.745328165108873 9 17 6.055011917952491 9 18 6.099908432749707 9 19 4.9108546821872565 9 20 4.7092593835340395 9 21 3.0335241125493866 9 22 3.466053249066121 9 23 3.550091463661822 9 24 4.11383162901712 9 25 3.4791183461094874 9 26 2.811467258061697 9 27 3.2829011120325973 9 28 1.6846017247997622 9 29 1.6220631491027078 9 30 2.999069901730749 9 31 1.2419924228160752 9 32 1.8353076847900525 9 33 1.4837961715314805 9 34 1.694416048870231 9 35 1.1835458082089119 9 36 1.0057360555845918 9 37 1.2807785750624774 9 38 1.8148910409610508 9 39 2.658560498907079 9 40 -0.38931434632357664 9 41 -4.548466399184502 9 42 5.499098894539319 9 43 8.489617477486394 9 44 7.951650453664204 9 45 5.72485789106387 9 46 9.52438021128923 9 47 9.340954323870907 9 48 9.841587610698355 9 49 12.137992598348013 9 50 9.952198883932226 9 51 9.585181547304884 9 52 10.197007628574372 9 53 9.21747388397499 9 54 9.630278620494767 9 55 9.985907252797556 9 56 10.229710948027673 10 1 1.4458333646226151 10 2 2.9445862430973975 10 3 3.2903429656580485 10 4 1.970566596442346 10 5 1.872544340339111 10 6 2.0311444385748203 10 7 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5.530622933412648 114 37 6.154835890296258 114 38 7.222130859965578 114 39 9.130657029827137 114 40 11.671771626527104 114 41 11.804474777286085 114 42 9.568480493332682 114 43 6.666088065721688 114 44 5.621333711582757 114 45 8.297256081109118 114 46 -2.1422864553774374 114 47 -6.405652010970723 114 48 -2.9721378794977227 114 49 -12.888402575105982 114 50 -1.8034572246650562 114 51 -0.9600673214733959 114 52 -0.6823287213502134 114 53 -1.0419529636871516 114 54 -1.5228133855384165 114 55 0.09525868567840234 114 56 4.935593300968851 115 1 0.1933823842304605 115 2 2.0181156548621075 115 3 1.9202449264423649 115 4 0.7181156160501914 115 5 0.8112698305351884 115 6 0.9919287523003106 115 7 0.8771081626355226 115 8 0.33635401015587263 115 9 0.7781209489611811 115 10 0.5249282813570773 115 11 -3.538375617118877 115 12 -6.989988412792133 115 13 -4.201203581560861 115 14 -9.86973848946225 115 15 -2.401414169854121 115 16 -14.110063991753869 115 17 -12.150870434988686 115 18 -2.431954312348328 115 19 -4.863655121734315 115 20 -3.719662185093412 115 21 -1.8733386325486485 115 22 -1.014338907796624 115 23 -1.7611830461421043 115 24 -0.8395997435318989 115 25 -1.454705183302277 115 26 0.6668594149244385 115 27 -0.018569476202699775 115 28 -0.040888471278670124 115 29 -0.09362312540709894 115 30 -0.5131850002300382 115 31 -1.5962428713015733 115 32 -7.267633491680538 115 33 -2.464733240233226 115 34 -2.3864663040709466 115 35 -2.8899836035557946 115 36 0.5081870359767464 115 37 -0.3515743661139936 115 38 2.408028295863012 115 39 3.8742467734168855 115 40 4.703822908578385 115 41 3.1378081106194173 115 42 -3.4763913015391097 115 43 -1.5422452676116443 115 44 -0.8914868012377575 115 45 -2.2187695599165203 115 46 -7.350619788710771 115 47 -8.940908421227133 115 48 -9.388804546164389 115 49 -9.734556421259828 115 50 -5.67525209645993 115 51 -4.93442629583237 115 52 -4.52848256750406 115 53 -4.314388861123049 115 54 -4.163839026564055 115 55 -2.952818237398521 115 56 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0.9913616291963443 116 39 3.8325801067502177 116 40 6.630906241911717 116 41 1.0232247772860852 116 42 -0.42430796820577754 116 43 -10.990161934278312 116 44 -3.3498201345710896 116 45 -2.197936226583188 116 46 -6.3297864553774374 116 47 -7.9825750878937995 116 48 -8.784637879497723 116 49 -8.297056421259828 116 50 -7.373168763126596 116 51 -4.3927596291657025 116 52 -5.15348256750406 116 53 -6.428972194456382 116 54 -9.518005693230723 116 55 -8.077818237398521 116 56 -7.376906699031149 117 1 -0.4197128538647803 117 2 1.7204966072430565 117 3 0.8309592121566496 117 4 0.771687044621622 117 5 0.614841259106619 117 6 0.46811922849079224 117 7 0.4485367340640991 117 8 0.7173063911082522 117 9 -0.037355241515008686 117 10 0.35230923373802625 117 11 -9.330042283785541 117 12 -14.793559841363564 117 13 -10.36191786727515 117 14 -10.584024203747962 117 15 -14.496652265092216 117 16 -14.092206848896726 117 17 -10.460394244512496 117 18 -4.485525740919758 117 19 -4.792226550305742 117 20 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1.7040130907595383 118 3 1.3913987725962116 118 4 -0.23380746087288884 118 5 -0.19834555408019838 118 6 -0.09781483744327346 118 7 -0.020327734800375197 118 8 0.528661702463566 118 9 0.2941465899868234 118 10 0.02813340956220145 118 11 -9.073632027375286 118 12 -13.300885848689571 118 13 -9.5121010174583 118 14 -2.1646102843340422 118 15 -17.696285964725917 118 16 -4.773525530215409 118 17 -9.855998640116894 118 18 -3.2620825174765358 118 19 -1.966219224298417 118 20 -3.7485083389395655 118 21 0.09461008540007043 118 22 -0.039979933437649606 118 23 0.9150990051399503 118 24 -3.964599743531899 118 25 0.5997819961849018 118 26 0.39442351748854065 118 27 0.7987382161049936 118 28 0.4078294774392788 118 29 1.1756076438236676 118 30 0.4002765382315019 118 31 -0.012909537968241125 118 32 -5.2355822096292535 118 33 -0.544861445361434 118 34 -1.8704406630453079 118 35 -1.7040861676583567 118 36 0.7998537026434143 118 37 0.4625281979885685 118 38 1.2221308599655742 118 39 3.2845031836732943 118 40 5.0948485496040234 118 41 5.150628623439932 118 42 1.8761728010249925 118 43 3.896857296490918 118 44 0.3136414038904505 118 45 3.028025311878352 118 46 -4.526901839992821 118 47 -3.367190472509183 118 48 -7.895214802574646 118 49 -6.42686411356752 118 50 -5.49576491697275 118 51 -5.152375013781086 118 52 -5.182328721350213 118 53 -6.58041450214869 118 54 -8.984351847076876 118 55 -5.904741314321598 118 56 -4.6028682374926895 119 1 -0.2649509491028752 119 2 1.1014489881954361 119 3 0.9619115931090292 119 4 -0.15688438394980864 119 5 -0.3553968361314759 119 6 -0.7580712476996894 119 7 -0.4978918373644774 119 8 -0.16364598984412737 119 9 -0.4718790510388189 119 10 -0.6834050519762584 119 11 -8.580042283785541 119 12 -14.573321746125469 119 13 -3.617870248227529 119 14 -4.453071822795582 119 15 -10.984747503187453 119 16 -6.2767306584205365 119 17 -7.942537101655354 119 18 -0.34862097901499567 119 19 0.5530115449323532 119 20 -0.9696621850934122 119 21 1.126661367451348 119 22 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120 5 0.7279364972018527 120 6 0.20026208563364634 120 7 0.5021081626355226 120 8 0.16968734348920833 120 9 -1.1385457177054832 120 10 -0.05840505197625845 120 11 -14.080042283785541 120 12 -4.073321746125469 120 13 -9.24287024822753 120 14 -6.61973848946225 120 15 -7.859747503187453 120 16 -10.235063991753869 120 17 -8.567537101655354 120 18 -3.556954312348328 120 19 -3.696988455067647 120 20 -5.63632885176008 120 21 -1.6650052992153164 120 22 -0.9310055744632884 120 23 -1.1361830461421007 120 24 -4.422933076865231 120 25 -1.121371849968945 120 26 1.1251927482577742 120 27 0.6897638571306359 120 28 1.0007781953880013 120 29 1.5730435412595654 120 30 1.7368149997699618 120 31 -0.7212428713015768 120 32 -12.809300158347202 120 33 -2.381399906899894 120 34 -8.594799637404282 120 35 -1.7233169368891268 120 36 -2.033479630689918 120 37 -2.5182410327806615 120 38 -2.716971704136988 120 39 1.3325801067502177 120 40 -0.879510424754951 120 41 -1.3621918893805827 120 42 -5.809724634872445 120 43 -5.625578600944978 120 44 -5.22482013457109 120 45 -9.760436226583188 120 46 -14.267286455377437 120 47 -14.565908421227132 120 48 -10.305471212831057 120 49 -14.776223087926496 120 50 -11.508585429793262 120 51 -10.726092962499036 120 52 -10.15348256750406 120 53 -12.397722194456382 120 54 -11.99717235989739 120 55 -11.744484904065187 120 56 -9.231073365697815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 190.0 5 52.0 6 27.0 7 50.0 8 95.0 9 146.0 10 88.0 11 75.0 12 84.0 13 83.0 14 101.0 15 157.0 16 202.0 17 243.0 18 375.0 19 425.0 20 533.0 21 650.0 22 786.0 23 924.0 24 1070.0 25 1102.0 26 1158.0 27 1179.0 28 1348.0 29 1444.0 30 1562.0 31 1767.0 32 2143.0 33 2515.0 34 3003.0 35 3335.0 36 3678.0 37 3069.0 38 1690.0 39 355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.55198297109567 1.0138920008962582 0.3669056688326238 0.06721935917544253 8 97.54089177683173 2.0361864216894463 0.2520725969079095 0.1708492045709164 9 92.68005714765947 4.372916491581925 1.7004230047342912 1.2466033560243157 10 45.9248263499888 35.589849876764504 7.394129509298677 11.091194263948017 11 35.77470311449697 20.78198521174098 24.879565314810666 18.563746358951377 12 40.35122115169168 12.180708043916647 21.941519157517362 25.526551646874303 13 23.563186197624916 16.390320412278736 24.96639032041228 35.08010306968407 14 27.10136403103381 13.797159902529199 29.913452651037726 29.188023415399268 15 28.334969841492498 19.514658437368496 25.6361341001543 26.51423762098471 16 32.47702823845809 17.679852084267146 27.414836396234872 22.428283281039892 17 33.40897629853757 21.348125735417717 21.6338880484115 23.609009917633216 18 36.62362398812358 24.245819444833476 19.086300103638553 20.044256463404388 19 26.16626040178196 35.336639488946794 17.987727998655124 20.509372110616123 20 29.43104462559879 24.9796901700423 20.377062498249153 25.21220270610976 21 25.66386554621849 24.834733893557424 28.952380952380953 20.54901960784314 22 25.146346245413554 21.765677954233535 31.467943870263003 21.620031930089908 23 31.058144745686754 26.187542012099485 23.582791844051087 19.17152139816267 24 30.682836656957203 26.34438718350885 20.66995294644858 22.302823213085368 25 24.918776607663006 28.01366793636567 20.384270669952947 26.683284786018373 26 25.237787940854634 21.21152605145759 30.40599309783676 23.144692909851013 27 26.909284473580996 19.171289292318036 25.65977475205917 28.259651482041797 28 21.779622980251347 31.115350089766608 24.18649012567325 22.918536804308797 29 27.542930778496792 27.01627587752472 25.590385746701404 19.85040759727708 30 22.83921217045359 20.99011010562295 35.35427114560278 20.816406578320677 31 25.98456140350877 20.401403508771928 34.037894736842105 19.576140350877193 32 34.86274509803921 17.35854341736695 28.647058823529413 19.131652661064425 33 25.98568822786586 24.33562508769468 33.11912445629297 16.559562228146486 34 19.310982788585523 21.27039300330773 38.26035768346695 21.158266524639792 35 24.45148093143161 27.74399641325973 33.11009611343066 14.694426541877995 36 24.94249649368864 30.05610098176718 29.1023842917251 15.899018232819074 37 30.117911285794495 25.067377877596858 28.717012914093203 16.09769792251544 38 23.98552959982052 26.102807145461178 33.795114837768864 16.11654841694944 39 22.56305001827537 23.895746056737988 25.45618129164675 28.085022633339896 40 12.982218840702227 15.07030743652605 22.024966889283398 49.92250683348832 41 16.251482799525505 18.194656272948087 24.65118906400045 40.90267186352596 42 16.4833623253465 22.827001851747937 32.924639470287865 27.7649963526177 43 12.929508611243785 14.71075773326216 24.53291377518051 47.82681988031354 44 14.198086662915024 14.130557118739448 23.624085537422623 48.04727068092291 45 20.90786743677452 27.160320753658944 31.279089328772503 20.652722480794033 46 22.96869306875193 23.599316124330837 32.47568597774601 20.95630482917122 47 15.278789576912299 19.4424208461754 37.56514429812272 27.713645278789578 48 20.703956955498263 21.057056383813475 39.98710906849008 18.251877592198184 49 14.224695104816501 14.772663406957792 23.20013488450514 47.80250660372056 50 16.980973227816133 21.036089128360555 41.1348711904361 20.848066453387215 51 21.598156837402716 21.898794639094152 36.41089039363884 20.09215812986429 52 17.13659235937368 27.04860427852585 34.83540887751947 20.979394484581 53 21.71240949654824 21.64505809058764 37.18639501599596 19.456137396868158 54 15.930891344589668 19.717282773321365 35.5724462893364 28.77937959275257 55 18.343245062836626 22.388352782764812 31.519299820466784 27.74910233393178 56 24.23817273696616 24.740445591784052 29.704248274313933 21.317133396935855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 8.0 9 16.0 10 28.0 11 40.0 12 43.5 13 51.5 14 56.0 15 64.5 16 73.0 17 88.0 18 103.0 19 117.0 20 131.0 21 168.0 22 253.0 23 301.0 24 341.0 25 381.0 26 477.0 27 573.0 28 663.0 29 852.5 30 952.0 31 999.0 32 1046.0 33 1008.0 34 970.0 35 894.0 36 818.0 37 793.5 38 704.5 39 640.0 40 686.0 41 732.0 42 741.0 43 750.0 44 762.5 45 775.0 46 817.0 47 1062.5 48 1266.0 49 1433.5 50 1601.0 51 1607.0 52 1613.0 53 1641.5 54 1780.0 55 1890.0 56 2073.0 57 2256.0 58 2399.0 59 2542.0 60 2640.0 61 2738.0 62 2684.5 63 2319.5 64 2008.0 65 1718.0 66 1428.0 67 1193.5 68 959.0 69 830.0 70 701.0 71 591.5 72 418.5 73 355.0 74 320.5 75 286.0 76 218.0 77 150.0 78 115.0 79 58.0 80 36.0 81 25.5 82 15.0 83 9.5 84 4.0 85 3.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.01960564642617074 10 0.0 11 0.0 12 0.0 13 0.0 14 0.002800806632310105 15 0.1652475913062962 16 0.02240645305848084 17 0.02800806632310105 18 0.008402419896930316 19 0.03641048622003137 20 0.01960564642617074 21 0.01120322652924042 22 0.002800806632310105 23 0.0 24 0.0 25 0.0 26 0.17645081783553665 27 0.02800806632310105 28 0.1568451714093659 29 0.01960564642617074 30 0.03080887295541116 31 0.22126372395249833 32 0.01120322652924042 33 0.19325565762939725 34 0.08402419896930316 35 0.047613712749271786 36 0.1512435581447457 37 0.23526775711404882 38 0.12603629845395475 39 0.3837105086264844 40 0.6077750392112928 41 0.8346403764284114 42 0.17365001120322654 43 0.3108895361864217 44 0.45933228769885726 45 0.106430652027784 46 0.07002016580775262 47 0.03921129285234148 48 0.0560161326462021 49 0.3304951826125924 50 0.1960564642617074 51 0.3164911494510419 52 0.3669056688326238 53 0.1960564642617074 54 0.14004033161550525 55 0.1568451714093659 56 0.18485323773246695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 35704.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.85021286130406 #Duplication Level Percentage of deduplicated Percentage of total 1 93.83404817956077 81.49507058032714 2 4.098810022896578 7.119650459332287 3 0.9610113192943983 2.503921129285234 4 0.34506111129027056 1.1987452386287252 5 0.2192911735302654 0.9522742549854357 6 0.1515688993518011 0.7898274703114497 7 0.06127253378051534 0.372507282097244 8 0.048373052984617367 0.33609679587721264 9 0.048373052984617367 0.3781088953618642 >10 0.19994195233641845 3.086488908805736 >50 0.029023831790770425 1.4704234819628053 >100 0.0032248701989744913 0.29688550302487116 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 106 0.29688550302487116 No Hit TAAGGGGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 73 0.2044588841586377 No Hit TAAGGGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 63 0.17645081783553665 No Hit TAAGGGGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 61 0.1708492045709164 No Hit TAAGGGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 58 0.1624467846739861 No Hit TAAGGGGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 56 0.1568451714093659 No Hit TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 55 0.1540443647770558 No Hit TAAGGGGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 54 0.1512435581447457 No Hit TAAGGGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 53 0.14844275151243558 No Hit TAAGGGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 52 0.14564194488012547 No Hit TAAGGGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 50 0.14004033161550525 No Hit TAAGGGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 45 0.12603629845395475 No Hit TAAGGGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 44 0.12323549182164464 No Hit TAAGGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCCAGGCTCACAACCAAA 43 0.12043468518933453 No Hit TAAGGGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 40 0.1120322652924042 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002800806632310105 0.0 0.0 0.0 0.0 11 0.002800806632310105 0.0 0.0 0.0 0.0 12 0.002800806632310105 0.0 0.0 0.0 0.0 13 0.008402419896930316 0.0 0.0 0.0 0.0 14 0.014004033161550527 0.0 0.0 0.0 0.0 15 0.01960564642617074 0.0 0.0 0.0 0.0 16 0.01960564642617074 0.0 0.0 0.0 0.0 17 0.01960564642617074 0.0 0.0 0.0 0.0 18 0.01960564642617074 0.0 0.0 0.0 0.0 19 0.025207259690790948 0.0 0.0 0.0 0.0 20 0.025207259690790948 0.0 0.0 0.0 0.0 21 0.028008066323101054 0.0 0.0 0.0 0.0 22 0.028008066323101054 0.0 0.0 0.0 0.0 23 0.03641048622003137 0.0 0.0 0.0 0.0 24 0.04201209948465158 0.0 0.0 0.0 0.0 25 0.04201209948465158 0.0 0.0 0.0 0.0 26 0.04201209948465158 0.0 0.0 0.0 0.0 27 0.04201209948465158 0.0 0.0 0.0 0.0 28 0.053215326013892 0.0 0.0 0.0 0.0 29 0.053215326013892 0.0 0.0 0.0 0.0 30 0.053215326013892 0.0 0.0 0.0 0.0 31 0.053215326013892 0.0 0.0 0.0 0.0 32 0.053215326013892 0.0 0.0 0.0 0.0 33 0.053215326013892 0.0 0.0 0.0 0.0 34 0.053215326013892 0.0 0.0 0.0 0.0 35 0.053215326013892 0.0 0.0 0.0 0.0 36 0.053215326013892 0.0 0.0 0.0 0.0 37 0.053215326013892 0.0 0.0 0.0 0.0 38 0.053215326013892 0.0 0.0 0.0 0.0 39 0.05601613264620211 0.0 0.0 0.0 0.0 40 0.05601613264620211 0.0 0.0 0.0 0.0 41 0.05601613264620211 0.0 0.0 0.0 0.0 42 0.05601613264620211 0.0 0.0 0.0 0.0 43 0.05601613264620211 0.0 0.0 0.0 0.0 44 0.061617745910822315 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCCG 15 0.008178086 50.28531 40 CACCACT 15 0.008178086 50.28531 41 GTACCCA 20 4.0425846E-4 50.214386 37 GGTACCC 20 4.070875E-4 50.143658 36 CACACAA 15 0.0083162 50.073135 45 ACCGTTC 15 0.0083162 50.073135 45 ACTTCGG 15 0.0083162 50.073135 45 CCTTTTC 15 0.0083162 50.073135 43 CACTTCG 15 0.0083162 50.073135 44 CGACTCC 30 1.0090116E-6 50.073135 44 ACTCCTC 35 5.0391463E-8 50.07313 46 CTACACC 15 0.008362622 50.002808 21 CCAATAG 15 0.008362622 50.002808 27 CTTTAAT 15 0.008362622 50.002808 32 AACGAGT 15 0.008362622 50.002808 29 GTCGTGC 15 0.008362622 50.002808 30 GCGGTGA 15 0.008362622 50.002808 23 GCGGTCG 15 0.008362622 50.002808 27 TCGCGCA 15 0.008362622 50.002808 50 TCGTGCT 15 0.008362622 50.002808 31 >>END_MODULE