FastQCFastQC Report
Fri 17 Jun 2016
SRR1526349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67094
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG3680.5484842161743225No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3050.45458610307926195No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2960.44117208692282467No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1960.29212746296241093No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1320.19673890362774615No Hit
AGTTTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC980.14606373148120547No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG950.14159239276239305No Hit
AGTTTAGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA780.11625480668912272No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG690.10284079053268548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCAC251.9972978E-550.2872237
GATCCTC150.00820838650.2872237
AAGCGAT251.9972978E-550.2872237
TGCCTGT354.9600203E-850.28721637
CCCAGTT150.00825733650.2117142
CCCGTGC150.00825733650.2117143
CCCGCAC150.00825733650.2117143
ACCAGCA252.0151629E-550.2117142
CGAATCT150.00828189250.1740444
CACCATA150.00828189250.1740444
CCAATGA150.00830650250.13643334
TCAAGCG252.0331601E-550.13643335
TTCAAGC301.0137337E-650.13643334
GTGGGCT150.00830650250.13643335
GTAAGAC150.00830650250.13643334
TGAAAAC150.00830650250.13643334
AAGTCAC204.0998973E-450.1364335
CTGCAAT150.008331167550.09887749
CGATTAA1200.050.09887749
AACGATT1300.050.0613847