FastQCFastQC Report
Fri 17 Jun 2016
SRR1526348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8549
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA220.2573400397707334No Hit
ATTTGCGGGGATCCAGTGACAGATACCATTAGATTCAAGCCATAGAATATGAAGGA140.1637618434904667No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC120.14036729442040005No Hit
ATTTGCGGGATGCTTCCCAGAGTAAACTGTCATGACTTTGCTTTTGGGGTCCATGC110.1286700198853667No Hit
ATTTGCGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA100.11697274535033338No Hit
ATTTGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA90.10527547081530003No Hit
ATTTGCGGGGATCCAGTGACAGATACCATTAGATTCAAGCGATAGAATATGAAGGA90.10527547081530003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATTT150.00798430150.25443641
ACCGTAA150.00798430150.25443639
GCTACCG150.00798430150.25443636
CCGTAAA150.00798430150.25443640
TACCGTA150.00798430150.25443638
CGTAAAC203.8530413E-450.25443641
CTACCGT150.00798430150.25443637
GCGGGAA1050.049.9588285
GCGGGGT750.049.9588245
GCGGGGC402.1991582E-949.9588245
GCGGGGA800.049.9588245
AACGTGT203.968245E-449.95882448
CAATGCT203.968245E-449.95882432
CAGTCTA203.968245E-449.95882420
TTGCAGG203.968245E-449.9588243
ATCTTTC203.968245E-449.95882410
AACAACG203.968245E-449.95882445
TAAACAA203.968245E-449.95882443
CCAGTCT203.968245E-449.95882419
CGGGGGG402.1991582E-949.9588246