FastQCFastQC Report
Fri 17 Jun 2016
SRR1526347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118668
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG5410.45589375400276405No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG4600.3876360939764722No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4300.3623554791519196No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3100.2612330198537095No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG3040.256176896888799No Hit
GGACATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC2610.21994134897360706No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG2000.16853743216368355No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1340.11292007954966797No Hit
GGACATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC1220.10280783361984697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC203.907112E-450.6600938
TATTTCT150.00798715350.66008838
TCACCGT150.00798715350.66008840
CCGGATC150.00798715350.66008840
ATTTGTT150.00798715350.66008839
CACCGTG150.00802790650.59494441
AATATTC150.00812359750.44359237
GGTACTC204.0414123E-450.3145836
TATGCCT1150.050.3145835
CGAATCT150.00826176850.22893544
ATTATAT150.00826176850.22893544
ATTCTAT150.00826176850.22893544
CGAATAT204.0841248E-450.20757743
CCAGAAC150.00827568150.20757343
CGGGTAT150.00828961150.18622634
TTGGGAC150.00828961150.18622634
CCACGAT750.050.10102546
CGATTTA150.00834550650.1010249
CGATTCA355.1526513E-850.1010249
CGATTAA1250.050.1010249