##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526347_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 118668 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.47204806687565 39.0 38.0 39.0 33.0 40.0 2 37.10479657531938 39.0 38.0 39.0 33.0 40.0 3 35.82874068830687 38.0 35.0 39.0 29.0 40.0 4 36.834260289210235 39.0 38.0 39.0 31.0 40.0 5 35.640914147032056 39.0 36.0 39.0 26.0 40.0 6 36.75317693059628 39.0 37.0 39.0 32.0 40.0 7 36.98745238817541 39.0 37.0 39.0 33.0 40.0 8 36.72225031179425 38.0 36.0 39.0 31.0 40.0 9 36.62084133886136 38.0 36.0 39.0 31.0 40.0 10 36.337774294670844 38.0 36.0 39.0 31.0 40.0 11 35.192705699935956 38.0 34.0 39.0 28.0 40.0 12 33.17444466916102 37.0 31.0 39.0 19.0 40.0 13 32.57468736306333 36.0 30.0 39.0 17.0 40.0 14 32.06835878248559 36.0 29.0 39.0 15.0 40.0 15 32.555608925742405 36.0 30.0 39.0 17.0 40.0 16 32.27885360838642 36.0 29.0 39.0 17.0 40.0 17 32.43144739946742 36.0 30.0 39.0 18.0 40.0 18 32.51409815620049 36.0 30.0 38.0 19.0 40.0 19 33.3092071999191 36.0 31.0 38.0 23.0 40.0 20 33.64468938551252 36.0 31.0 39.0 24.0 40.0 21 35.13402939292817 38.0 35.0 39.0 27.0 40.0 22 34.80412579633937 38.0 34.0 39.0 25.0 40.0 23 35.04873259851013 38.0 35.0 39.0 27.0 40.0 24 34.62251828631139 38.0 33.0 39.0 25.0 40.0 25 34.931422118852595 38.0 34.0 39.0 27.0 40.0 26 35.34859439781575 38.0 35.0 39.0 28.0 40.0 27 35.312957157784744 38.0 35.0 39.0 28.0 40.0 28 35.14649273603668 38.0 35.0 39.0 28.0 40.0 29 35.05018202042674 38.0 35.0 39.0 27.0 40.0 30 34.81732227727778 38.0 35.0 39.0 27.0 40.0 31 34.618650352243236 38.0 35.0 39.0 26.0 40.0 32 33.48289345063539 38.0 33.0 39.0 18.0 40.0 33 34.69607644857923 38.0 35.0 39.0 26.0 40.0 34 34.48420804260626 38.0 35.0 39.0 26.0 40.0 35 34.44589948427546 38.0 35.0 39.0 26.0 40.0 36 32.228865406006676 35.0 31.0 38.0 21.0 39.0 37 32.003733104122425 35.0 31.0 38.0 18.0 39.0 38 31.225199716857112 35.0 30.0 38.0 11.0 39.0 39 29.423812653790407 34.0 27.0 38.0 4.0 39.0 40 25.183790069774496 31.0 11.0 37.0 4.0 39.0 41 21.7212727946877 25.0 9.0 33.0 4.0 37.0 42 25.551302794350626 30.0 20.0 34.0 4.0 37.0 43 21.574560959989213 26.0 8.0 33.0 4.0 36.0 44 22.84991741665824 28.0 9.0 33.0 4.0 37.0 45 25.27302221323356 30.0 19.0 34.0 4.0 37.0 46 25.441028752485927 31.0 15.0 36.0 4.0 38.0 47 24.59021808743722 30.0 9.0 36.0 4.0 38.0 48 24.537272053122997 30.0 10.0 35.0 4.0 38.0 49 22.026047460140898 27.0 4.0 35.0 4.0 38.0 50 24.36620655947686 30.0 4.0 35.0 4.0 38.0 51 25.78207267334075 32.0 4.0 38.0 4.0 39.0 52 25.675590723699734 32.0 4.0 38.0 4.0 40.0 53 25.20559881349648 32.0 4.0 38.0 4.0 39.0 54 24.317827889574275 31.0 4.0 38.0 4.0 39.0 55 24.50905046010719 31.0 4.0 37.0 4.0 39.0 56 23.774159840900666 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.679080953908354 1 2 1.073570823351396 1 3 2.191482736697459 1 4 1.4164493321369847 1 5 3.2177899778216243 1 6 1.4464606987116326 1 7 1.7006850513262037 1 8 1.7601528115180827 1 9 1.755608782025213 1 10 1.5710982230405435 1 11 -4.526329155091567 1 12 -8.125813192085452 1 13 -22.32197059055698 1 14 -18.78413806865209 1 15 -12.70153677436916 1 16 -21.57002516509705 1 17 -21.51256409074162 1 18 -20.543420166329682 1 19 -6.511467304847674 1 20 -11.005611368869673 1 21 -8.641154825089242 1 22 -4.091623612186364 1 23 -4.802248574703199 1 24 -5.18293402882794 1 25 -4.576761752616964 1 26 1.5031028904071562 1 27 1.2729374733322345 1 28 1.3819615991957903 1 29 0.8933451866112634 1 30 1.2312337691048967 1 31 0.39319437347352704 1 32 -13.459139173846285 1 33 -0.6932625337157887 1 34 -1.2011065520181674 1 35 -0.03796320684381982 1 36 2.354145658603912 1 37 2.6525660701039726 1 38 3.9603625935475932 1 39 5.669562497795351 1 40 7.447122652273716 1 41 -7.230119443201511 1 42 -11.922002878670098 1 43 -14.508619930268278 1 44 -16.43655571613055 1 45 -17.022985864831057 1 46 -18.98335797131872 1 47 -18.08846262856947 1 48 -17.55119383472514 1 49 -15.178857899491508 1 50 -17.479814304072356 1 51 -18.752318692729695 1 52 -18.583893659146497 1 53 -18.447785875236384 1 54 -18.025361944270685 1 55 -17.88212771912041 1 56 -17.04176514583225 2 1 0.9050397674356532 2 2 1.2568317339880863 2 3 2.618650694377415 2 4 1.5237608756850705 2 5 3.286465587116922 2 6 1.6451193044172783 2 7 1.8041235550103139 2 8 2.0386338241763085 2 9 2.0700807261022973 2 10 1.5802612685723787 2 11 -3.662452336652116 2 12 -12.454265865427601 2 13 -14.094783739999638 2 14 -16.818239712332424 2 15 -9.213911609056481 2 16 -5.303352200804589 2 17 -3.5116194468723556 2 18 -11.523110323140564 2 19 -2.974626193946481 2 20 -4.1846213820946865 2 21 2.4671013623281013 2 22 2.901763880728808 2 23 2.5707912892680795 2 24 2.8444606433806356 2 25 2.181409416852148 2 26 2.3615952387918213 2 27 2.5232680986864793 2 28 2.1370154438963382 2 29 2.37227962832673 2 30 2.6174041828589054 2 31 -2.7860990329122686 2 32 -11.07353554641383 2 33 -3.131199431505319 2 34 -2.453515393884782 2 35 -2.1661513799025762 2 36 2.894576416208295 2 37 3.082473495004791 2 38 3.6451349343751005 2 39 5.631871207411823 2 40 8.06378617012749 2 41 1.3281650835319105 2 42 -2.100729498734335 2 43 -5.7683025299282065 2 44 -11.884505838934253 2 45 -4.825838689316228 2 46 -13.72669823204043 2 47 -13.24659601228381 2 48 -11.439631393765383 2 49 -13.352483442661732 2 50 -11.475185549112975 2 51 -11.869737925678871 2 52 -11.721906128445573 2 53 -11.737791543099599 2 54 -15.566076095035847 2 55 -11.500113738391146 2 56 -10.646242757772551 3 1 1.599976476296412 3 2 2.0313205363249907 3 3 3.0357230734751113 3 4 2.261378532290067 3 5 4.059006969784882 3 6 2.1921170324276673 3 7 2.70409109801583 3 8 2.767845119210392 3 9 2.982933695917289 3 10 2.3141139138174225 3 11 1.6691932329681336 3 12 1.624052862258214 3 13 -5.44875972182372 3 14 -5.4297944023681275 3 15 -2.572107000163264 3 16 2.510571849828324 3 17 2.44621891729512 3 18 2.2973375833832925 3 19 1.016967444482603 3 20 1.031931685091294 3 21 3.2061471266903254 3 22 3.4148440495051773 3 23 3.2003920682359492 3 24 3.0653629478272464 3 25 3.1260702412598107 3 26 2.580485209580523 3 27 2.586332038965608 3 28 2.3969635127051703 3 29 2.7296635945714556 3 30 2.8171120699085677 3 31 2.8602820122029513 3 32 1.8242898349558558 3 33 2.6698067353236326 3 34 3.060278828770201 3 35 2.7533877307757777 3 36 4.766468658986611 3 37 5.000097643008679 3 38 5.843447170662017 3 39 7.957966630975601 3 40 10.640157478144364 3 41 9.392689588562746 3 42 7.270610975137785 3 43 2.0298798783807825 3 44 2.9208495651553292 3 45 6.879386886795423 3 46 0.20952377380182696 3 47 -4.179507404688874 3 48 0.26851531184967925 3 49 -9.974456827926257 3 50 -0.8820339749487438 3 51 -2.627251719901526 3 52 -2.4136295948525834 3 53 -2.642887291233322 3 54 -8.382499334243898 3 55 -3.0184843972681357 3 56 0.09734698581719314 4 1 1.355745707065644 4 2 1.9274743824788416 4 3 2.909120509372549 4 4 2.35689135280289 4 5 3.883045431323346 4 6 2.2350657503763856 4 7 2.6364628928876286 4 8 2.7313066576719294 4 9 2.3242798497634425 4 10 1.9262934009969115 4 11 3.2682316945065963 4 12 3.3705272212325745 4 13 4.6384197653557635 4 14 3.0570645719908462 4 15 5.632059666503398 4 16 6.065379542136014 4 17 6.278911224987432 4 18 5.362722198767909 4 19 4.582031547046704 4 20 4.476162454322065 4 21 2.9705702036133985 4 22 3.036318408479538 4 23 3.0061612990051785 4 24 3.4477347426990406 4 25 2.7767112669008327 4 26 2.589139055734371 4 27 2.3937038338374066 4 28 2.464912230653887 4 29 2.6424841073919723 4 30 2.036342839139337 4 31 2.916371755792696 4 32 3.938392399058422 4 33 2.3499349404518384 4 34 3.0522660082573765 4 35 2.962682602570645 4 36 5.075443017960971 4 37 5.252020719931757 4 38 6.229665119379966 4 39 8.392582015590985 4 40 10.261631837118724 4 41 7.642369075742231 4 42 8.753944308471116 4 43 8.304238852739758 4 44 11.572772642078405 4 45 9.532912527821065 4 46 9.156639158417214 4 47 9.81280028761882 4 48 8.575887106721474 4 49 5.636440607971178 4 50 9.143607050692282 4 51 7.040376485226677 4 52 7.416178097455109 4 53 6.170253734407705 4 54 1.3091673324227706 4 55 7.3299130386293 4 56 6.666257242227449 5 1 1.8107872871072246 5 2 1.8034619084663674 5 3 2.481599719351756 5 4 1.7517458226573623 5 5 3.990399901177817 5 6 2.2090262493368797 5 7 2.6282768222015562 5 8 2.5358284871937613 5 9 2.551332864316457 5 10 1.4478630475665568 5 11 3.593517557292458 5 12 5.715251753457107 5 13 5.818045544981544 5 14 5.8343774618037365 5 15 5.844247816191551 5 16 5.980842120098593 5 17 6.022934094010296 5 18 5.881952967998675 5 19 4.759448386963541 5 20 4.583698836858446 5 21 3.297207417750613 5 22 3.25448368914482 5 23 2.991660259504144 5 24 3.273540356004652 5 25 3.1924597076492773 5 26 1.8179072470025588 5 27 1.9204969731305397 5 28 1.982687698429352 5 29 2.0503843152921775 5 30 2.1293781821746762 5 31 2.193476745397689 5 32 3.7068955175615415 5 33 1.6362655017823968 5 34 1.8543190228103938 5 35 1.712500689888735 5 36 3.0194139119318635 5 37 3.6645467074577454 5 38 3.87407780129265 5 39 5.696428169437137 5 40 4.1392565772434615 5 41 0.5871455830187386 5 42 7.007686512213322 5 43 9.686983135484041 5 44 9.597772642078404 5 45 6.5935934010019395 5 46 7.49933666361472 5 47 8.490762865581399 5 48 8.303303946638312 5 49 8.04522439175496 5 50 7.310343017428249 5 51 6.35958126693146 5 52 6.691983710760724 5 53 6.8867038383578105 5 54 7.098720346975785 5 55 7.178743599959862 5 56 6.656459944930152 6 1 1.7606147741687508 6 2 2.170109407028754 6 3 2.2465795928422665 6 4 2.2629879158143424 6 5 4.04313135603693 6 6 2.352700774926298 6 7 2.831123285686317 6 8 2.786748556198937 6 9 2.8888256762773565 6 10 1.9727336628627086 6 11 3.7681294031809003 6 12 6.108149970823408 6 13 5.598321565683101 6 14 6.437281429601324 6 15 6.116818258974757 6 16 6.283976105147467 6 17 6.268477509766477 6 18 5.839399776509314 6 19 4.614048731989421 6 20 4.47636703697345 6 21 3.2045104654791956 6 22 3.4570426310654625 6 23 2.6810794659446273 6 24 3.180038343353708 6 25 2.8223331981610613 6 26 2.211581772591977 6 27 2.14257862925475 6 28 1.6674490555311365 6 29 2.4771813250679102 6 30 2.004018780219532 6 31 2.0727751927812434 6 32 3.4535315152613677 6 33 2.350957853708792 6 34 2.9067054517925683 6 35 2.5480958595264553 6 36 3.217832543329216 6 37 2.8577899507009903 6 38 3.9001847593144987 6 39 4.870896254543521 6 40 0.6610794639599682 6 41 -0.03878886206464216 6 42 5.633649709453117 6 43 10.499001536864146 6 44 8.894581152716704 6 45 6.284651480357891 6 46 9.673517227156985 6 47 10.040705361268571 6 48 9.161095781025892 6 49 10.474206565417987 6 50 9.258991666076898 6 51 8.058891215177578 6 52 8.318694464067221 6 53 8.22457042835206 6 54 8.89897911638349 6 55 7.74449978167349 6 56 8.15162958265298 7 1 1.7228680425614726 7 2 2.133452139661415 7 3 2.972547293431859 7 4 2.5666920942301346 7 5 4.242695570340956 7 6 2.3612242304505244 7 7 2.9042455620257215 7 8 2.9529238958554345 7 9 3.0422478757134 7 10 2.6710802406632723 7 11 3.706542630558495 7 12 5.812776989536562 7 13 6.390041637459568 7 14 7.451700809247566 7 15 6.7084614273931145 7 16 6.985270645009045 7 17 6.064939955293269 7 18 5.7674951366733715 7 19 5.4389013338863705 7 20 5.06038395571224 7 21 3.5926160794243387 7 22 3.7212697523164238 7 23 3.1571112526659775 7 24 3.721309812207842 7 25 3.190636197392031 7 26 2.174924505224638 7 27 2.7331964195649263 7 28 2.7658853539161683 7 29 2.922836285334512 7 30 2.5185022459975386 7 31 2.8323240264136444 7 32 3.978012417594101 7 33 2.6746569052340448 7 34 2.8440292149302238 7 35 2.771243770318563 7 36 1.202644130101838 7 37 1.3788742880503904 7 38 2.2852142389351116 7 39 4.0976329887142455 7 40 -1.1136577828998142 7 41 -9.726433055861104 7 42 5.541942454903001 7 43 9.88672263401872 7 44 8.384519630030212 7 45 5.542469988432742 7 46 7.296178083347705 7 47 7.975914282058117 7 48 7.640588218862344 7 49 9.79758518628443 7 50 7.957786846799792 7 51 9.233205493567734 7 52 8.734933889855476 7 53 8.800640666752468 7 54 9.673972707770314 7 55 9.488219340760907 7 56 9.540504230179256 8 1 1.7921113077570823 8 2 2.0197388595747796 8 3 2.513971416891991 8 4 2.2895836604951967 8 5 3.9428444805886897 8 6 2.5590718005060253 8 7 2.8605877848495993 8 8 2.810714609270889 8 9 2.777661440083236 8 10 1.5644740405820485 8 11 3.627620199260271 8 12 6.1570980941798545 8 13 6.558039471492329 8 14 7.108868807081603 8 15 6.4421828298569 8 16 7.0015830857833805 8 17 6.672493766041882 8 18 6.449368698335757 8 19 4.941744165888537 8 20 5.254576456050671 8 21 3.197936236456961 8 22 3.9669039745988073 8 23 2.9799080578643 8 24 3.796103368455306 8 25 3.385505562492881 8 26 2.8870539217672118 8 27 2.313269520959267 8 28 2.476796413886383 8 29 3.122172957867342 8 30 2.614346296356274 8 31 2.520898549223986 8 32 3.732039762930505 8 33 1.9611060726903844 8 34 2.363793665992901 8 35 2.5221575377478302 8 36 0.5004646255668455 8 37 0.36621691699312464 8 38 1.4014996051189499 8 39 2.998675360448374 8 40 -3.7066590012563836 8 41 -10.793510353816973 8 42 3.6841517414875398 8 43 7.781334790509852 8 44 6.332604102752562 8 45 3.6396000818400793 8 46 7.040120143724042 8 47 7.738470123401015 8 48 7.623801972754315 8 49 9.626609147297021 8 50 7.657868070571279 8 51 9.314090832676978 8 52 9.795445599615867 8 53 10.138328496724043 8 54 10.555386001394247 8 55 10.188977429294813 8 56 9.376229152339807 9 1 1.2678336191535564 9 2 1.5002765802810387 9 3 2.5215289342809726 9 4 1.6705360414475763 9 5 3.509190119968032 9 6 1.6352489005595316 9 7 1.9411790101037454 9 8 2.2125337638990317 9 9 2.308025271008866 9 10 1.5540406537441669 9 11 3.3810979948728956 9 12 5.723778136983487 9 13 5.941533318469318 9 14 6.413978491404766 9 15 5.907471754415486 9 16 6.518905183161657 9 17 6.416273862350067 9 18 5.893857729903434 9 19 4.725117627632784 9 20 4.5420965202561305 9 21 2.9822460277892233 9 22 3.567225001886129 9 23 2.6535971964410763 9 24 3.0569380394023398 9 25 2.7419127321023 9 26 2.601272755368072 9 27 1.962614090247662 9 28 2.090827981569639 9 29 2.052282642190505 9 30 2.6366175644140597 9 31 2.72475087667182 9 32 3.4544180400840645 9 33 1.9653195558364516 9 34 2.393154286645661 9 35 2.5705122729003165 9 36 1.2393624318803802 9 37 2.0423137602248005 9 38 2.397018599233448 9 39 3.8619043599133285 9 40 -1.4872234742365897 9 41 -1.953555832682678 9 42 6.234713539240349 9 43 10.180612479113385 9 44 8.97753454684031 9 45 6.257042564451101 9 46 9.492032931310987 9 47 10.352635452453981 9 48 9.968973187307554 9 49 12.106678703209273 9 50 9.568515475600709 9 51 9.063957071307268 9 52 9.192048060825073 9 53 8.815629192283161 9 54 8.998269896525333 9 55 8.46017860639487 9 56 8.18709057556078 10 1 1.892192045158687 10 2 2.2371243133862535 10 3 2.937304525954765 10 4 2.4243141994173527 10 5 4.155183846800085 10 6 2.4960629866730173 10 7 2.8210264580649635 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2.0952993334030765 115 30 1.6618384775215702 115 31 0.8631897573787413 115 32 -4.369894674692571 115 33 0.9253711022282047 115 34 0.9756898782018695 115 35 0.3994887088355128 115 36 2.529249520736542 115 37 2.561398198123669 115 38 3.7850279266757667 115 39 5.378902396241262 115 40 8.22847362934711 115 41 5.581851629271178 115 42 1.1831671474877723 115 43 -1.591478831637719 115 44 -3.369237667199947 115 45 0.9480096729439822 115 46 -3.447890976396426 115 47 -8.420744518090935 115 48 -5.708064122461714 115 49 -8.893198567286554 115 50 -2.663688746294234 115 51 -1.3908784711571442 115 52 -0.9757231713791512 115 53 -1.5794984464011783 115 54 -1.9922799316533606 115 55 -1.604315748047938 115 56 0.23004590202126352 116 1 0.7272492035691371 116 2 1.1739778789823347 116 3 2.5314495692016052 116 4 1.5572604281719649 116 5 2.688843799621715 116 6 1.3439429842536157 116 7 1.814736008660745 116 8 1.9167184680837366 116 9 1.9130036259872227 116 10 0.7639973637008737 116 11 -1.615655251880348 116 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35 -1.2395526621646198 119 36 1.332436024953978 119 37 0.3675302104412488 119 38 2.579377906592754 119 39 3.9327918058007754 119 40 6.337992975979862 119 41 5.980967976841132 119 42 1.182765487292297 119 43 -0.6343225858216783 119 44 -3.8411883968826324 119 45 -1.667199859791321 119 46 -7.732534168256114 119 47 -10.028858054039523 119 48 -10.59974975641539 119 49 -8.089208742678172 119 50 -7.4617875547023225 119 51 -9.496648989298796 119 52 -9.367475748698737 119 53 -11.226037474383505 119 54 -12.69545304719761 119 55 -12.133436112219849 119 56 -9.386502498032291 120 1 0.35224920356913714 120 2 0.8557960608005146 120 3 2.132459670211709 120 4 1.06231093322247 120 5 2.707783193561106 120 6 0.6520237923344254 120 7 1.0180188369435683 120 8 1.214698266063536 120 9 1.0948218078054026 120 10 0.3397549394584516 120 11 -10.880806767031864 120 12 -3.8222175340121822 120 13 -7.250566248630246 120 14 -7.876064798638524 120 15 -9.051943830000095 120 16 -11.734882695626226 120 17 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120 55 -11.537656891440628 120 56 -7.896242757772551 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1028.0 5 50.0 6 93.0 7 149.0 8 279.0 9 360.0 10 229.0 11 201.0 12 197.0 13 235.0 14 296.0 15 419.0 16 602.0 17 840.0 18 1009.0 19 1211.0 20 1573.0 21 1918.0 22 2484.0 23 2877.0 24 3305.0 25 3489.0 26 3656.0 27 3977.0 28 4294.0 29 4381.0 30 4892.0 31 5465.0 32 6512.0 33 8025.0 34 9576.0 35 11472.0 36 13074.0 37 12046.0 38 6565.0 39 1889.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.62163346479252 0.2553342097279806 0.0716284086695655 0.05140391680992348 8 99.16405433646813 0.5789260794822529 0.12977382276603633 0.1272457612835811 9 96.14107254244146 2.2489336952307095 0.7990963804642851 0.8108973818635467 10 37.3487376546331 38.654902753901645 12.956315097583174 11.04004449388209 11 27.66565262457128 33.10945755770349 22.344038359442813 16.88085145828242 12 35.46196110156066 14.858260019550343 18.51805035898473 31.16172851990427 13 25.113762766710483 15.889709104392086 21.303131425489603 37.69339670340783 14 23.409868676140867 18.835449012963807 26.739324668318755 31.01535764257658 15 28.888063606745558 20.402944006482215 22.03953476594811 28.66945762082412 16 29.112291350531105 18.765806777946384 26.88248187489462 25.239419996627888 17 29.302396143246046 22.962688265387822 23.797082149870626 23.937833441495503 18 29.25371876448527 24.94037335131263 23.979604736420715 21.826303147781385 19 28.234649584896122 29.713852248303763 18.42049812465759 23.631000042142524 20 24.696332386435476 30.94248649195418 19.119469287634974 25.241711833975373 21 24.568485992650775 23.734113205002867 29.33958129656474 22.357819505781613 22 24.265381277018886 24.331954123724373 22.376903435664506 29.02576116359224 23 24.272760980213707 28.27299693261873 22.40705160616173 25.04719048100583 24 21.94188829338996 29.3145582633903 25.246064650958978 23.49748879226076 25 27.850202667970038 28.34402150555757 21.708646885823356 22.097128940649043 26 24.06747606423397 26.38675469850222 25.344050252444234 24.20171898481957 27 22.959884686385745 22.34201276204766 26.096448711572666 28.60165383999393 28 20.0244839376926 31.573304065177933 27.200810502764995 21.20140149436447 29 25.221693978016052 27.16636320722908 27.87106345674017 19.740879358014702 30 19.542913986562244 26.07885618903904 36.9091476214161 17.46908220298261 31 19.420911709307138 29.761050062081374 33.86855641803147 16.949481810580018 32 26.92226651720621 22.215105057775457 31.608357423998516 19.254271001019816 33 20.075319389676515 25.960702192875058 33.70880443134705 20.255173986101376 34 18.539946529927217 28.647814389690563 36.09651601150365 16.715723068878564 35 19.92277080803993 26.95686631593147 39.509982463240256 13.610380412788345 36 19.516807077375024 30.248518512265537 34.08350357076531 16.15117083959413 37 18.706488678607638 26.21324771882393 39.2429874957756 15.837276106792835 38 23.43291139240506 27.209282700421937 36.26244725738397 13.095358649789029 39 16.571650905892106 23.880533892713952 30.86207771556172 28.68573748583222 40 11.142852298928522 17.52678692526787 27.712600027126 43.61776074867761 41 14.584679201659242 18.728536941960492 30.147733841079866 36.539050015300404 42 19.1179575531852 27.94851655729813 36.35004687222884 16.583479017287832 43 9.901534040485146 16.544816802603222 30.423023285297724 43.130625871613915 44 11.124662279475908 17.07785993173599 25.787873398209555 46.00960439057855 45 15.516164956803454 27.57154427645788 40.12553995680346 16.786750809935207 46 17.490956006982216 25.985141710305516 37.230893774191095 19.29300850852117 47 14.947150154250746 26.607832229134004 35.924408705473795 22.520608911141455 48 16.077690855138925 30.92797410304829 37.57671297545185 15.41762206636094 49 11.896489047824359 19.607395571787265 25.302019664037466 43.19409571635091 50 18.73025793483218 26.81204709379909 39.37602405364774 15.081670917720984 51 18.980651490038223 31.284883130940706 35.25098941244123 14.483475966579846 52 15.878346939638762 30.218687872763418 38.05422782454211 15.84873736305571 53 17.718696687667855 28.541627958042664 38.54872219313211 15.190953161157376 54 14.409732475048722 27.806227906623583 32.9500299499709 24.834009668356803 55 16.276007596539355 30.690018991348385 35.95357670394598 17.08039670816628 56 21.279721696838692 27.338894893272087 34.01107808700351 17.370305322885706 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 6.5 9 12.0 10 21.0 11 30.0 12 92.5 13 254.0 14 353.0 15 427.0 16 501.0 17 563.5 18 626.0 19 742.0 20 858.0 21 1003.0 22 1327.0 23 1506.0 24 1908.0 25 2310.0 26 2742.0 27 3174.0 28 3745.0 29 4670.0 30 5024.0 31 5214.0 32 5404.0 33 5232.5 34 5061.0 35 4695.5 36 4330.0 37 3938.5 38 3248.5 39 2950.0 40 2760.0 41 2570.0 42 2531.5 43 2493.0 44 2493.5 45 2494.0 46 3018.0 47 4442.0 48 5342.0 49 5215.0 50 5088.0 51 4874.5 52 4661.0 53 4565.5 54 4657.0 55 4844.0 56 4931.5 57 5019.0 58 5190.5 59 5362.0 60 5633.5 61 5905.0 62 5662.0 63 5230.0 64 5041.0 65 4773.0 66 4505.0 67 3919.0 68 3333.0 69 2909.5 70 2486.0 71 2129.5 72 1502.0 73 1231.0 74 1012.5 75 794.0 76 650.0 77 506.0 78 379.5 79 200.5 80 148.0 81 105.0 82 62.0 83 37.0 84 12.0 85 10.0 86 8.0 87 4.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.028651363467826204 10 0.0 11 8.426871608184178E-4 12 0.0 13 0.0 14 0.025280614824552534 15 0.16011056055549938 16 0.04044898371928405 17 0.01601105605554994 18 0.01095493309063943 19 0.01938180469882361 20 0.029494050628644623 21 0.013482994573094684 22 8.426871608184178E-4 23 0.0 24 0.0 25 8.426871608184178E-4 26 0.1904472983449624 27 0.029494050628644623 28 0.18791923686250717 29 0.03033673778946304 30 0.039606296558465634 31 0.23173896922506487 32 0.01601105605554994 33 0.20140223143560185 34 0.08174065459938652 35 0.05056122964910507 36 0.17359355512859406 37 0.25954764553207266 38 0.1415714430174942 39 0.37246772508174064 40 0.5898810125728925 41 0.8629116526780598 42 0.21994134897360706 43 0.29746856776890146 44 0.5030842350085953 45 0.1179762025145785 46 0.06825766002629184 47 0.025280614824552534 48 0.03707823507601038 49 0.3210638082718172 50 0.22415478477769915 51 0.35055785890046176 52 0.3901641554589274 53 0.22078403613442543 54 0.11544814103212322 55 0.16263862203795462 56 0.19887416995314658 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 118668.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.96197795530387 #Duplication Level Percentage of deduplicated Percentage of total 1 93.7703239792846 78.73141874810395 2 3.9895218595688307 6.699362928506421 3 1.0036532980047372 2.528061482455253 4 0.42053073186398493 1.4123436815316681 5 0.22180737885904694 0.9311693127043515 6 0.12746396884660163 0.6421276165436344 7 0.07928861054237425 0.46600599993258507 8 0.06222650447629371 0.41797283176593525 9 0.03613151872817054 0.27303064010516737 >10 0.24790236460717008 3.637037786092291 >50 0.029105945642137383 1.6609363939731014 >100 0.01104018627805211 2.144638824282873 >500 0.0010036532980047374 0.45589375400276405 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 541 0.45589375400276405 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 460 0.3876360939764722 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 430 0.3623554791519196 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 310 0.2612330198537095 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 304 0.256176896888799 No Hit GGACATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 261 0.21994134897360706 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 200 0.16853743216368355 No Hit GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 134 0.11292007954966797 No Hit GGACATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC 122 0.10280783361984697 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 8.426871608184178E-4 0.0 0.0 0.0 0.0 10 0.010112245929821012 0.0 0.0 0.0 0.0 11 0.021067179020460444 0.0 0.0 0.0 0.0 12 0.025280614824552534 0.0 0.0 0.0 0.0 13 0.027808676307007785 0.0 0.0 0.0 0.0 14 0.03370748643273671 0.0 0.0 0.0 0.0 15 0.03876360939764722 0.0 0.0 0.0 0.0 16 0.04381973236255773 0.0 0.0 0.0 0.0 17 0.04803316816664981 0.0 0.0 0.0 0.0 18 0.05730272693565241 0.0 0.0 0.0 0.0 19 0.061516162739744494 0.0 0.0 0.0 0.0 20 0.06994303434792867 0.0 0.0 0.0 0.0 21 0.07331378299120235 0.0 0.0 0.0 0.0 22 0.07668453163447601 0.0 0.0 0.0 0.0 23 0.08174065459938652 0.0 0.0 0.0 0.0 24 0.09269558769002595 0.0 0.0 0.0 0.0 25 0.09775171065493646 0.0 0.0 0.0 0.0 26 0.10365052078066539 0.0 0.0 0.0 0.0 27 0.1188188896753969 0.0 0.0 0.0 0.0 28 0.1272457612835811 0.0 0.0 0.0 0.0 29 0.13061650992685475 0.0 0.0 0.0 0.0 30 0.1323018842484916 0.0 0.0 0.0 0.0 31 0.13482994573094684 0.0 0.0 0.0 0.0 32 0.13482994573094684 0.0 0.0 0.0 0.0 33 0.13482994573094684 0.0 0.0 0.0 0.0 34 0.13482994573094684 0.0 0.0 0.0 0.0 35 0.13482994573094684 0.0 0.0 0.0 0.0 36 0.13567263289176526 0.0 0.0 0.0 0.0 37 0.13567263289176526 0.0 0.0 0.0 0.0 38 0.13567263289176526 0.0 0.0 0.0 0.0 39 0.13567263289176526 0.0 0.0 0.0 0.0 40 0.13567263289176526 0.0 0.0 0.0 0.0 41 0.13651532005258368 0.0 0.0 0.0 0.0 42 0.13988606869585735 0.0 0.0 0.0 0.0 43 0.14241413017831261 0.0 0.0 0.0 0.0 44 0.14915562746485994 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGACC 20 3.907112E-4 50.66009 38 TATTTCT 15 0.007987153 50.660088 38 TCACCGT 15 0.007987153 50.660088 40 CCGGATC 15 0.007987153 50.660088 40 ATTTGTT 15 0.007987153 50.660088 39 CACCGTG 15 0.008027906 50.594944 41 AATATTC 15 0.008123597 50.443592 37 GGTACTC 20 4.0414123E-4 50.31458 36 TATGCCT 115 0.0 50.31458 35 CGAATCT 15 0.008261768 50.228935 44 ATTATAT 15 0.008261768 50.228935 44 ATTCTAT 15 0.008261768 50.228935 44 CGAATAT 20 4.0841248E-4 50.207577 43 CCAGAAC 15 0.008275681 50.207573 43 CGGGTAT 15 0.008289611 50.186226 34 TTGGGAC 15 0.008289611 50.186226 34 CCACGAT 75 0.0 50.101025 46 CGATTTA 15 0.008345506 50.10102 49 CGATTCA 35 5.1526513E-8 50.10102 49 CGATTAA 125 0.0 50.10102 49 >>END_MODULE