FastQCFastQC Report
Fri 17 Jun 2016
SRR1526346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51821
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1120.21612859651492636No Hit
CGCACCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC1040.2006908396210031No Hit
CGCACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1030.19876112000926266No Hit
CGCACCGGGGATGAGATGGACTTAAATGAATTGACGGGTCCGCACAGTTCTGAGTC870.16788560622141602No Hit
CGCACCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC850.1640261669979352No Hit
CGCACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA760.14665869049227145No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG650.12543177476312692No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG580.11192373748094402No Hit
CGCACCGGGGATGAGATGGACTCAAATGAATTGACGGGTCCGCACAGTTCTGAGTC520.10034541981050155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACC150.00800001350.59823638
AACAACC251.9193181E-550.59823238
CAACCTG203.928741E-450.54857641
CAACCAC203.928741E-450.54857640
TACTTTT150.00803116750.54857639
CTATCAA150.00803116750.54857641
TACAAAC203.9862574E-450.40019637
AAACCCA204.044441E-450.2526836
TATGCTC150.00825177750.20370535
CATCACT150.00825177750.20370544
TCAGACT150.00825177750.20370535
CACCACA150.00825177750.20370544
GTGCGTA252.0105088E-550.20370544
GTGCGCA150.00825177750.20370544
CGATTCA150.00825177750.20370549
CCACACA204.0639847E-450.203744
CTGCAAT204.0639847E-450.203749
CCCACAC150.00828365750.1548243
CCACCAC150.00828365750.1548243
TCAAAAC252.0221558E-550.1548243