##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51821
Sequences flagged as poor quality	0
Sequence length	56
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.59001177128963	39.0	38.0	40.0	33.0	40.0
2	36.73161459639914	39.0	36.0	39.0	30.0	40.0
3	36.74153335520349	39.0	38.0	39.0	30.0	40.0
4	35.15912467918412	39.0	36.0	39.0	19.0	40.0
5	36.994095057988076	39.0	38.0	40.0	31.0	40.0
6	36.94986588448698	39.0	38.0	40.0	32.0	40.0
7	36.51091256440439	38.0	36.0	39.0	31.0	40.0
8	36.66484629783292	38.0	36.0	39.0	31.0	40.0
9	36.71951525443353	38.0	36.0	39.0	31.0	40.0
10	36.17400281739063	38.0	36.0	39.0	29.0	40.0
11	35.25190559811659	38.0	34.0	39.0	28.0	40.0
12	33.13118233920611	37.0	31.0	39.0	19.0	40.0
13	32.59987263850562	37.0	30.0	39.0	16.0	40.0
14	32.12805619343509	36.0	29.0	39.0	15.0	40.0
15	32.6183593523861	36.0	30.0	39.0	17.0	40.0
16	32.25968236815191	36.0	29.0	39.0	17.0	40.0
17	32.3872947260763	36.0	29.0	39.0	18.0	40.0
18	32.43300978367843	36.0	29.0	39.0	18.0	40.0
19	33.39630651666313	36.0	31.0	39.0	23.0	40.0
20	33.502865633623436	36.0	30.0	39.0	23.0	40.0
21	35.15439686613535	38.0	35.0	39.0	27.0	40.0
22	34.704598521834775	38.0	34.0	39.0	25.0	40.0
23	35.00638737191486	38.0	34.0	39.0	27.0	40.0
24	34.504428706508946	38.0	33.0	39.0	24.0	40.0
25	34.88761312981224	38.0	34.0	39.0	26.0	40.0
26	35.313135601397114	38.0	35.0	39.0	28.0	40.0
27	35.30202041643349	38.0	35.0	39.0	28.0	40.0
28	35.278516431562494	38.0	35.0	39.0	28.0	40.0
29	35.091372223615906	38.0	35.0	39.0	27.0	40.0
30	34.92559001177129	38.0	35.0	39.0	27.0	40.0
31	34.7032863124988	38.0	35.0	39.0	26.0	40.0
32	33.70421257791243	38.0	33.0	39.0	19.0	40.0
33	34.85104494316976	38.0	35.0	39.0	27.0	40.0
34	34.645722776480575	38.0	35.0	39.0	26.0	40.0
35	34.75504139248567	38.0	35.0	39.0	27.0	40.0
36	32.50172709905251	35.0	31.0	38.0	23.0	39.0
37	32.242469269215185	35.0	31.0	38.0	22.0	39.0
38	31.615773528106367	35.0	30.0	38.0	17.0	39.0
39	29.43947434437776	34.0	27.0	38.0	6.0	39.0
40	25.60267073194265	31.0	12.0	37.0	4.0	39.0
41	22.18932479110785	26.0	10.0	32.0	4.0	36.0
42	26.253526562590455	30.0	23.0	34.0	4.0	38.0
43	22.08147276200768	27.0	8.0	33.0	4.0	37.0
44	23.29235252117867	28.0	10.0	34.0	4.0	37.0
45	25.91667470716505	30.0	22.0	34.0	4.0	38.0
46	26.014511491480288	31.0	18.0	36.0	4.0	38.0
47	25.126647498118523	30.0	13.0	36.0	4.0	38.0
48	25.112657030933406	30.0	15.0	35.0	4.0	38.0
49	22.184770652824145	27.0	5.0	35.0	4.0	38.0
50	24.931996680882268	30.0	13.0	35.0	4.0	38.0
51	26.51637367090562	33.0	4.0	38.0	4.0	40.0
52	26.22205283572297	32.0	4.0	38.0	4.0	40.0
53	25.774415777387546	32.0	4.0	38.0	4.0	40.0
54	24.899905443739026	31.0	4.0	38.0	4.0	40.0
55	25.196271781710116	32.0	4.0	38.0	4.0	40.0
56	24.7701510970456	32.0	4.0	38.0	4.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.2907385401165143
1	2	2.2453486909583873
1	3	2.4669498916896586
1	4	4.610424581774609
1	5	2.08663747097593
1	6	2.201574080556476
1	7	2.4607439538672935
1	8	2.1635002215701675
1	9	2.1582765893401543
1	10	2.2834445032859776
1	11	-4.946378237815981
1	12	-7.800388733559938
1	13	-21.163969734819624
1	14	-18.982940335821866
1	15	-11.167223126581494
1	16	-21.750619899541707
1	17	-21.754410078091595
1	18	-20.53085806265099
1	19	-5.810476322283169
1	20	-10.83079484262948
1	21	-7.368368688688598
1	22	-2.4253968647802218
1	23	-3.43316614470951
1	24	-3.3757677201336698
1	25	-3.23849221639567
1	26	3.403615616135241
1	27	2.659486085615711
1	28	3.0136535500410417
1	29	2.8386068494810104
1	30	2.7234107752289916
1	31	2.509909795634698
1	32	-12.51555291045656
1	33	1.821958947456693
1	34	1.631072330934117
1	35	1.8799944101677113
1	36	4.64741588115135
1	37	4.871746038603948
1	38	5.8314466915318235
1	39	7.587028258280874
1	40	9.534629455892944
1	41	-2.359668385328657
1	42	-7.42516360254514
1	43	-14.477970792014588
1	44	-16.37774304192449
1	45	-16.199257666059108
1	46	-18.90165767662332
1	47	-18.50938975142982
1	48	-17.187450506956626
1	49	-14.795010653908136
1	50	-17.043766215969296
1	51	-18.15175009823221
1	52	-18.188640788016908
1	53	-17.747910129557777
1	54	-17.974007299556902
1	55	-17.039311749579213
1	56	-16.302550761447705
2	1	0.9518296363594345
2	2	1.7836914598724363
2	3	1.7399812864400417
2	4	4.047635081002603
2	5	1.6983204354638346
2	6	1.8006476780860723
2	7	1.8325916121277146
2	8	1.7479057799849897
2	9	1.6634232697313038
2	10	1.8235886103369268
2	11	-4.139636086503579
2	12	-11.282375352190922
2	13	-13.23988327058905
2	14	-16.250310382141986
2	15	-6.2969194724384145
2	16	-4.908880321569498
2	17	-2.809479558276877
2	18	-10.502551320499677
2	19	-2.4903528019537795
2	20	-3.230691909021658
2	21	2.021235016921281
2	22	3.0591630940463332
2	23	2.5205137317701585
2	24	2.7979327430675625
2	25	2.825326620454561
2	26	1.996513197195462
2	27	2.1602580876743787
2	28	1.6322845330570033
2	29	1.8239388103662435
2	30	1.8904205539217358
2	31	-2.3017217020492957
2	32	-9.292701649519866
2	33	-1.93177239582689
2	34	-2.3390769227972257
2	35	-1.6727590638415535
2	36	4.04371027126972
2	37	3.858107335567407
2	38	5.108338096575565
2	39	7.777455432753335
2	40	9.803028838291294
2	41	6.491849885386731
2	42	2.6474045909700443
2	43	-1.6493552490398073
2	44	-10.41531380877987
2	45	0.21324876729137898
2	46	-13.035728700812715
2	47	-11.824366591368058
2	48	-10.16737845343115
2	49	-12.443749717212306
2	50	-9.486380729608
2	51	-10.314642532612035
2	52	-9.960900180708622
2	53	-10.357019753850109
2	54	-15.315489543509555
2	55	-9.813887148446943
2	56	-8.845525542713787
3	1	1.7714130380323923
3	2	2.482559755755659
3	3	2.6069650489461935
3	4	4.849340837932246
3	5	2.4119500893919934
3	6	2.5770951112499247
3	7	2.252142210782793
3	8	2.3785437986827063
3	9	2.6794967484913528
3	10	2.8808298765334186
3	11	1.550211379463292
3	12	2.081574131161549
3	13	-4.339604444937091
3	14	-2.477635296034066
3	15	-3.945927181147617
3	16	2.5364206461195273
3	17	3.6317210321774454
3	18	2.871567098394859
3	19	2.1269999945034925
3	20	1.5841350550590363
3	21	3.514920435979839
3	22	4.021931668755208
3	23	3.88134692842425
3	24	4.0162368270432935
3	25	3.5455168779582493
3	26	3.2020241041989053
3	27	3.4472844941037692
3	28	2.9071738228716626
3	29	3.3814260991968155
3	30	2.892224719905002
3	31	3.5459082799090424
3	32	2.6648184423285883
3	33	2.9922886177863646
3	34	3.8398635397253287
3	35	2.541608657988853
3	36	5.2813681357932545
3	37	5.234193935534602
3	38	6.531333012107794
3	39	8.668877443578332
3	40	10.706095920462854
3	41	10.939119034148419
3	42	8.463051630497677
3	43	5.004408897261655
3	44	5.722294851216851
3	45	8.62131830968928
3	46	1.0587439906749019
3	47	-2.44762393104331
3	48	1.8887147071396235
3	49	-9.692888637993018
3	50	0.7356957014236549
3	51	-0.9431483551477093
3	52	-0.39800039392823905
3	53	-0.7329423387279164
3	54	-6.987459364733107
3	55	-1.3428358117239654
3	56	2.8103707997497196
4	1	1.8624470040663539
4	2	2.812354960550863
4	3	2.6750219920031384
4	4	5.102587591178995
4	5	2.5329540853959926
4	6	2.7105866197414343
4	7	2.533236116876701
4	8	2.5216506917896027
4	9	2.481944300938906
4	10	2.805779926483467
4	11	3.282853737105647
4	12	2.1392664388538556
4	13	5.408522428189784
4	14	2.9900220466232774
4	15	6.094532359311923
4	16	6.722734332433216
4	17	5.950527225983642
4	18	6.022041623869388
4	19	5.346655339158836
4	20	4.96113305705704
4	21	3.8287316247910255
4	22	4.274054545878087
4	23	3.8810971781744996
4	24	4.587790273596738
4	25	3.3022601347015055
4	26	2.851499628674425
4	27	3.002354424173703
4	28	2.9435124842103235
4	29	2.6991084168791275
4	30	2.8366552893355745
4	31	3.2379662219669854
4	32	3.9969862744964146
4	33	3.003777129274873
4	34	3.176526876388664
4	35	3.09080945718965
4	36	5.2516478560729745
4	37	5.367435693776361
4	38	6.287951393726175
4	39	7.937733587434479
4	40	10.026400615767553
4	41	2.9604726805020647
4	42	6.486278403724452
4	43	6.416621684474443
4	44	10.023119027041027
4	45	7.957482145853117
4	46	8.686116618047532
4	47	8.884419025999648
4	48	9.124853568278482
4	49	5.744174299069918
4	50	8.950356041083996
4	51	7.88939410239475
4	52	7.923428177500334
4	53	7.672152566366986
4	54	2.0722309449572016
4	55	7.1350612911731375
4	56	7.425505664884586
5	1	2.4385833677027193
5	2	2.9794004150963147
5	3	3.577294719275862
5	4	5.395769409360817
5	5	3.237499539941446
5	6	3.357177528832338
5	7	2.5275542986948807
5	8	3.2182416008805106
5	9	2.8410352100298155
5	10	3.273961744665286
5	11	3.7135355552874643
5	12	6.751766438853853
5	13	6.790340610007966
5	14	6.550249319350552
5	15	7.392259632039195
5	16	7.842052514251396
5	17	7.217572680529095
5	18	7.461814351142113
5	19	6.354609884613382
5	20	6.196360329784312
5	21	4.301458897518302
5	22	4.313827273150814
5	23	4.0095062690835945
5	24	5.454835728142193
5	25	3.9477146801560536
5	26	4.117408719583516
5	27	3.831899878719156
5	28	3.6912397569375983
5	29	3.9377447805154944
5	30	4.001428016608301
5	31	3.691375312876076
5	32	4.975395365405511
5	33	3.1844589474566902
5	34	3.9060723309341157
5	35	3.9317185480987433
5	36	4.357329674254796
5	37	4.617435693776361
5	38	5.191360484635268
5	39	6.836597223798115
5	40	3.8979915248584582
5	41	2.77524540777479
5	42	8.744232949178997
5	43	11.958667139019894
5	44	11.510619027041024
5	45	8.45634578221675
5	46	10.347480254411163
5	47	11.083282662363285
5	48	10.220308113733026
5	49	12.603265208160831
5	50	10.451492404720362
5	51	9.431439556940202
5	52	9.410928177500331
5	53	10.230107111821535
5	54	7.819958217684476
5	55	7.930515836627684
5	56	8.216414755793675
6	1	1.997187263806616
6	2	2.245796518992421
6	3	2.7708012127823594
6	4	5.316873305464711
6	5	2.7456164230583298
6	6	3.072599606754416
6	7	2.3660283246689033
6	8	2.2976247177636253
6	9	2.530645599640202
6	10	2.930779926483467
6	11	3.531230360482269
6	12	6.687967737555152
6	13	6.312743207410563
6	14	6.34554152714276
6	15	6.802324567104133
6	16	7.222734332433216
6	17	7.17617657663299
6	18	6.228210455038219
6	19	4.718408585912087
6	20	5.58613305705704
6	21	3.5478874689468682
6	22	3.8730805199040574
6	23	3.1700582171355407
6	24	4.449803260609727
6	25	3.074987407428779
6	26	2.505720407895204
6	27	2.870860917680197
6	28	3.3737072894051323
6	29	3.0627447805154944
6	30	2.734382562062848
6	31	3.7087454427462063
6	32	3.8216615991717404
6	33	3.9193615448592922
6	34	3.747955447817233
6	35	2.4447055610857547
6	36	3.8198296742547946
6	37	3.433994135334803
6	38	4.4194449002196805
6	39	5.682863457564345
6	40	3.544257758624692
6	41	1.1828752779046603
6	42	7.625888793334841
6	43	12.298115190967948
6	44	10.092924221846221
6	45	6.5354042237751955
6	46	9.60819454012545
6	47	10.24967876625939
6	48	10.048554866979785
6	49	10.400018454914076
6	50	9.854576820304771
6	51	10.241666829667476
6	52	10.212389216461375
6	53	10.418905813120237
6	54	10.867685490411748
6	55	9.980840511952358
6	56	9.104077093456013
7	1	2.2399722565916065
7	2	2.961344859540759
7	3	3.346739163720308
7	4	5.115213853805258
7	5	2.9458328732747816
7	6	3.0085664177212266
7	7	2.7844987431393236
7	8	2.9724082675471735
7	9	3.1410352100298127
7	10	3.4156284113319515
7	11	4.212146666398581
7	12	6.62537754996497
7	13	7.090340610007964
7	14	7.582193763794994
7	15	6.839481854261418
7	16	7.668441403140282
7	17	7.543961569417981
7	18	7.013203240031
7	19	5.961554329057826
7	20	6.136638107562092
7	21	3.5597922308516345
7	22	4.667993939817478
7	23	3.8747840468613717
7	24	4.236780172586634
7	25	4.5143813468227165
7	26	3.3590753862501828
7	27	3.637455434274713
7	28	3.3690175347153755
7	29	4.048855891626609
7	30	3.7861502388305226
7	31	3.1622086462094146
7	32	4.18511758762773
7	33	4.1691811696789145
7	34	3.506072330934117
7	35	4.1122741036543005
7	36	2.792051896477016
7	37	2.7841023604430255
7	38	3.142749373524154
7	39	5.310208334909227
7	40	-2.1478418084748725
7	41	-7.965032370002987
7	42	6.406732949178998
7	43	9.829500472353228
7	44	8.731452360374359
7	45	6.232734671105643
7	46	9.641924698855608
7	47	9.256893773474399
7	48	8.336974780399697
7	49	11.00048743038305
7	50	8.577881293609249
7	51	9.121717334717982
7	52	8.25676151083367
7	53	8.52316266737709
7	54	8.247735995462254
7	55	6.708293614405463
7	56	6.516414755793676
8	1	2.129252381787225
8	2	2.4799285841104037
8	3	3.1223651418110734
8	4	4.617952507952367
8	5	2.8906685540259502
8	6	2.980593021790085
8	7	2.6780824677089683
8	8	2.4906007558100853
8	9	2.2959647874946043
8	10	3.0447363925526076
8	11	4.190824287681835
8	12	6.361097424769351
8	13	7.0815377931065555
8	14	6.827890164420975
8	15	6.684161040489901
8	16	6.784834204392244
8	17	7.2096501453178305
8	18	6.701779139874507
8	19	5.566405659261271
8	20	5.760268780488538
8	21	3.4835011510394267
8	22	4.400975160474758
8	23	2.5600344380976807
8	24	4.67789910842388
8	25	3.544897778747604
8	26	2.2117749167666148
8	27	2.69105480829662
8	28	2.0947608836981573
8	29	2.3303504143183105
8	30	2.245266044777317
8	31	3.1422555945662225
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115	17	-9.476024880446516
115	18	0.3926070340689449
115	19	-0.982890115386617
115	20	-1.7021152799717854
115	21	1.3910930438597617
115	22	2.626327273150814
115	23	1.1732257812787168
115	24	2.112457679361704
115	25	2.3477146801560522
115	26	2.719847743973766
115	27	3.100192561645983
115	28	3.0363617081571093
115	29	2.9651838049057417
115	30	3.481610943437566
115	31	2.578875312876079
115	32	-4.997470488253029
115	33	1.8585443133103539
115	34	0.8993650138609439
115	35	1.3341575724889907
115	36	4.014951625474307
115	37	4.114386913288559
115	38	5.223372679757219
115	39	7.400316735993236
115	40	9.408052500468212
115	41	8.263964919969915
115	42	3.7085622174716804
115	43	0.19738665121501953
115	44	-0.5165151193004363
115	45	4.288967733436266
115	46	-1.921422184613224
115	47	-6.415192947392814
115	48	-4.436399203340141
115	49	-7.797954304034294
115	50	-0.8780807660113474
115	51	1.6274761423060582
115	52	2.3685501287198463
115	53	0.8639485752361651
115	54	1.4730069981722806
115	55	1.646979251261829
115	56	5.059097682622944
116	1	0.9582262248455748
116	2	1.9514242246201263
116	3	2.0630090049901497
116	4	2.7302932188846256
116	5	1.7196423970843
116	6	2.1375346716894867
116	7	1.6733876320282164
116	8	1.7997892199281296
116	9	1.5219875909821923
116	10	2.7251522208557617
116	11	0.035559364811277305
116	12	-0.25359070400328676
116	13	-4.879897485230131
116	14	-8.957488775887544
116	15	-2.6327403679608032
116	16	-11.571637961939079
116	17	-8.719927319470905
116	18	-2.423304696476933
116	19	-3.578128210624712
116	20	-4.688758717834734
116	21	2.5002684213278243
116	22	1.5072796541031934
116	23	1.5315300786074033
116	24	2.441145251951717
116	25	2.3000956325370012
116	26	2.6269325291073287
116	27	2.0938046406239152
116	28	2.555525471223312
116	29	2.2294114471821587
116	30	1.9508327785130604
116	31	0.5312562652570278
116	32	-4.07877130126116
116	33	1.0362446617424084
116	34	1.6548818547436426
116	35	1.1698137861939841
116	36	3.7722106266357436
116	37	3.778149979490646
116	38	4.361003341778122
116	39	6.472906747607638
116	40	8.453348667715606
116	41	5.336554931584317
116	42	1.2817329491789984
116	43	-10.110975718122962
116	44	-1.054261925339926
116	45	1.617655306026279
116	46	-5.034662602731693
116	47	-8.997074480493858
116	48	-8.252310933886019
116	49	-6.299115744220124
116	50	-2.7514837857558305
116	51	0.2308443188449658
116	52	0.578190082262239
116	53	-2.163345269130847
116	54	-4.333613210886952
116	55	-2.4563889252770785
116	56	0.7426052319841503
117	1	0.43441670103605645
117	2	1.4381967113926137
117	3	1.0921095340906817
117	4	3.235584224175632
117	5	1.0106476880895912
117	6	0.8465293806841956
117	7	0.6363505949911783
117	8	0.18074160088050917
117	9	0.5854796544742555
117	10	0.8600728557764015
117	11	-10.45914962989772
117	12	-11.240363190775774
117	13	-9.617992723325369
117	14	-7.491880310279079
117	15	-3.1327403679608032
117	16	-7.039891930193047
117	17	-3.9791865787301646
117	18	-1.8756856488578855
117	19	-1.8717790042755063
117	20	-3.363361892437908
117	21	1.532014453073856
117	22	2.0337346805582186
117	23	1.7405247876021122
117	24	-3.008590197783736
117	25	1.273640606081976
117	26	1.9655568677316637
117	27	2.0170850639043394
117	28	1.470869386567223
117	29	2.1368188545895705
117	30	0.11485394253422498
117	31	0.4677642017649646
117	32	-9.634326856816713
117	33	-0.19655957106182598
117	34	0.5199612198230028
117	35	0.4317185480987433
117	36	2.486496340921459
117	37	2.473917175257842
117	38	2.263119743894528
117	39	6.4120601867610745
117	40	-0.6524714381045023
117	41	-2.020587925558541
117	42	-3.921970754524704
117	43	-8.79086989801714
117	44	-4.546325417403416
117	45	-5.017265328894357
117	46	-7.973816041885131
117	47	-8.965328448747826
117	48	-12.038025219600305
117	49	-7.809697754802135
117	50	-6.116563150835198
117	51	-10.36439377639313
117	52	-10.27101626694411
117	53	-11.245355851141431
117	54	-13.293930671204414
117	55	-11.472261941150094
117	56	-9.41877042939151
118	1	0.4246127794674237
118	2	1.2595474739198451
118	3	1.542000601628807
118	4	3.3619458799490474
118	5	1.1860289517061489
118	6	1.2593834111852829
118	7	1.3400542986948807
118	8	1.2689768949981541
118	9	1.1998587394415807
118	10	1.2195499799594032
118	11	-7.97984679765371
118	12	-9.904851208204967
118	13	-10.31407115469792
118	14	-3.479897739472978
118	15	-17.132740367960803
118	16	-7.271918073983898
118	17	-9.808162613588554
118	18	-3.6109797665049435
118	19	-2.8064195271513235
118	20	-3.4940808466862734
118	21	0.5352824269300669
118	22	1.1557390378566978
118	23	0.7514180337894771
118	24	-3.141487801269573
118	25	1.1418323272148783
118	26	1.4644675431129315
118	27	2.03778223166033
118	28	1.253004462819952
118	29	1.5039212511037263
118	30	2.159516251902417
118	31	1.3729929599349049
118	32	-10.059163458123905
118	33	1.027841300397867
118	34	0.719307625051762
118	35	0.2552479598634463
118	36	3.4963002624900916
118	37	4.257141576129303
118	38	4.9450369552235
118	39	6.860861929680468
118	40	8.781079760152576
118	41	7.136274819539498
118	42	1.2817329491789984
118	43	-0.01853874333304617
118	44	-0.48423391413544437
118	45	0.505610488099105
118	46	-5.706931510294719
118	47	-9.265982043519069
118	48	-9.087044827443442
118	49	-8.31732302713329
118	50	-8.002184065867876
118	51	-8.501648678353915
118	52	-9.061865940146724
118	53	-10.576510535237292
118	54	-11.993277076433172
118	55	-10.723895928078198
118	56	-8.55711465597103
119	1	0.23011562576724032
119	2	1.3999649312253482
119	3	1.1805205257274807
119	4	2.775608119038232
119	5	1.3141124431672537
119	6	0.6398388191549245
119	7	0.04973171804972054
119	8	0.18074160088050917
119	9	0.04426101648142833
119	10	1.3941230349878708
119	11	-8.37073863826092
119	12	-8.191378722436468
119	13	-2.7040142287017126
119	14	-4.700960358068805
119	15	-2.971450045380159
119	16	-5.595447485748604
119	17	-8.848959577535421
119	18	-2.1660082295030456
119	19	-1.5312772121608091
119	20	-1.1733977347318145
119	21	-0.475154005707509
119	22	0.07794017637662165
119	23	-0.03605824704543892
119	24	-7.856857820244905
119	25	-0.2974466101665314
119	26	0.7528925905512622
119	27	0.5415772980739959
119	28	0.2605945956472766
119	29	1.449841554709046
119	30	1.156669952092173
119	31	-2.001769848414245
119	32	-9.734685279755784
119	33	-2.161912020285243
119	34	-2.401185733582011
119	35	-1.406991129320609
119	36	2.0456361258676985
119	37	2.637596984098945
119	38	3.2619250007643004
119	39	5.223290772185209
119	40	7.697588299052011
119	41	4.8611325045489835
119	42	0.8462490782112582
119	43	-0.9796393125930081
119	44	-0.7470422632815534
119	45	0.8035232015715934
119	46	-3.9678423262339955
119	47	-5.6606689505399395
119	48	-9.736949950783101
119	49	-3.9435089853875596
119	50	-3.851330175924801
119	51	-6.740737862414637
119	52	-8.439475048306118
119	53	-14.18561869463008
119	54	-12.082461137154235
119	55	-14.629968034340058
119	56	-12.427133631303098
120	1	0.5772738438931952
120	2	1.5466623198582212
120	3	1.5868185287996752
120	4	3.0636265522179613
120	5	1.0053566827985918
120	6	1.2089632431180561
120	7	1.3638638225044062
120	8	0.9902654104043194
120	9	1.0934161624107617
120	10	1.844199839903382
120	11	-9.035869206617296
120	12	-3.0393049897175715
120	13	-7.308468913801562
120	14	-2.957488775887544
120	15	-5.894645129865566
120	16	-5.000209390510509
120	17	-7.8389749385185254
120	18	-2.35187612504836
120	19	-1.8638424963389966
120	20	-3.498282527358544
120	21	-0.33306491200551136
120	22	0.4120415588650985
120	23	-1.039898492821166
120	24	-3.225521414714951
120	25	-0.10466627222489677
120	26	0.26978967196446746
120	27	1.1652332120524918
120	28	-0.2063792906814541
120	29	1.3008400186107352
120	30	-0.0015481738678886359
120	31	-2.683029449028684
120	32	-9.007342729832587
120	33	-2.2970886715909273
120	34	-10.29749909763731
120	35	-2.187329070948877
120	36	-0.3944560400309207
120	37	-0.817088115747449
120	38	-0.3294728486980638
120	39	1.7824305571314447
120	40	-3.3085560941891607
120	41	0.07465016967955407
120	42	-6.718267050821002
120	43	-1.6824042895515312
120	44	-4.292357163435163
120	45	-12.310916122545152
120	46	-12.606091174160264
120	47	-13.211360194779573
120	48	-10.157072838647924
120	49	-15.180068125172504
120	50	-9.632436166708212
120	51	-10.24534615734551
120	52	-10.54085753678538
120	53	-10.830011935797515
120	54	-12.214565591839335
120	55	-11.384960353848506
120	56	-7.019299529920611
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	443.0
5	21.0
6	24.0
7	52.0
8	98.0
9	133.0
10	83.0
11	66.0
12	60.0
13	101.0
14	89.0
15	161.0
16	282.0
17	357.0
18	438.0
19	484.0
20	626.0
21	770.0
22	1078.0
23	1229.0
24	1361.0
25	1503.0
26	1560.0
27	1718.0
28	1785.0
29	1868.0
30	2173.0
31	2551.0
32	2943.0
33	3414.0
34	4404.0
35	5098.0
36	5653.0
37	5196.0
38	2747.0
39	1252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.24933907103298	0.5075162578877289	0.0675401864109145	0.1756044846683777
8	98.99847552150672	0.810482236930974	0.1099940178692036	0.08104822369309739
9	94.41741950428538	3.264226700640877	1.3087792448459579	1.0095745502277815
10	41.01812006715424	35.29457169873218	10.845024217981129	12.842284016132455
11	24.67194133539174	33.43689695098418	21.640293323041295	20.250868390582784
12	28.866675672024854	16.81364697709423	23.9458906620868	30.37378668879412
13	27.075895872329752	17.408000617510275	22.811215530383436	32.70488797977654
14	19.081538105165624	23.729827812524128	23.98656474403521	33.20206933827504
15	25.273472227590737	24.13899733292103	21.734760929225775	28.852769510262455
16	21.59939776478082	27.959542146814137	23.27870751056807	27.162352577836973
17	21.763547938860583	30.80708661417323	22.199706654315268	25.229658792650916
18	25.981820638014554	33.81901692494741	19.06710153038578	21.132060906652256
19	25.46703720858422	35.16867376872009	16.5064844835572	22.857804539138492
20	21.43891280427774	37.21792174198406	17.394745478061115	23.948419975677083
21	20.87152864889901	32.286701275643125	21.220834861145956	25.620935214311906
22	21.43422296840927	32.69071190104016	22.17333410525097	23.7017310252996
23	21.875301518689337	35.900503656818664	21.48935759634125	20.73483722815075
24	18.747226028058122	39.10190849269601	19.37824434109724	22.772621138148626
25	20.2311804094865	40.00887671021401	19.636826769070453	20.123116111229038
26	18.824325630861452	38.61548873634342	23.544426181958812	19.015759450836313
27	20.358252745767945	31.177254039029474	26.616094350184337	21.84839886501824
28	18.615622583139984	34.86078886310905	27.797757153905643	18.725831399845323
29	16.539263377345378	35.65554783414408	29.105860551308776	18.699328237201758
30	14.133477480260236	37.12619934747775	33.377092221857566	15.363230950404446
31	13.573224995163475	34.347069065583284	36.7111627007158	15.368543238537436
32	18.108306314651845	28.643662189285163	36.49065925582832	16.757372240234677
33	18.43636996499913	31.67482064471216	34.70500647806161	15.1838029122271
34	14.295373321742492	35.49116198203419	37.187288708586884	13.026175987636435
35	15.478626867976985	31.648453488821097	37.900142873691934	14.972776769509982
36	16.852868408844905	33.433199319622695	35.94209061388588	13.771841657646513
37	16.655702178708253	32.32653535079912	37.88166092643473	13.136101544057894
38	19.310824652610016	33.67605279941248	36.75473010842046	10.258392439557042
39	13.503720353433577	28.75910711517594	34.843435126337	22.89373740505348
40	8.60820439146557	22.46015259469219	26.60700071832107	42.32464229552117
41	11.70674715217603	23.175932236393727	28.713854541914124	36.403466069516114
42	15.69662856148088	34.87881777210391	37.03553260217799	12.38902106423722
43	7.750106510709168	19.900073589217243	28.907006468104885	43.44281343196871
44	8.604466173874242	23.638515414314263	26.570048309178745	41.186970102632756
45	11.59151501101194	34.08678180904911	38.53599165410919	15.78571152582976
46	12.17774172991136	36.11803101403936	38.41801363381805	13.286213622231235
47	14.828112031192697	31.470650684270467	34.43743123516127	19.263806049375567
48	12.038377637497346	35.29468543078319	39.92007876295824	12.746858168761221
49	8.7673779189095	22.88076520930953	28.27324478178368	40.07861208999729
50	12.142608426509845	34.18578558440051	39.51909312492746	14.152512864162183
51	14.728216651815117	35.61659757467746	38.119406454612374	11.535779318895045
52	14.957455468765144	36.23165933363053	35.807183145000295	13.003702052604035
53	12.668213457076567	39.561098221191024	35.64385150812065	12.126836813611755
54	14.499082214278813	32.04134866196503	32.47415708627186	20.985412037484302
55	12.396822021611799	35.59374456322128	39.55075293344416	12.458680481722759
56	14.552556268852967	34.594709567638645	34.67205506999768	16.180679093510715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	4.5
13	8.5
14	9.0
15	59.5
16	110.0
17	259.0
18	408.0
19	663.5
20	919.0
21	1049.5
22	1255.0
23	1330.0
24	1357.5
25	1385.0
26	1420.5
27	1456.0
28	1564.0
29	1914.5
30	2157.0
31	2370.5
32	2584.0
33	2702.5
34	2821.0
35	2815.0
36	2809.0
37	2672.5
38	2272.0
39	2008.0
40	1765.0
41	1522.0
42	1314.0
43	1106.0
44	989.5
45	873.0
46	856.5
47	765.0
48	690.0
49	765.5
50	841.0
51	1091.0
52	1341.0
53	1472.5
54	1605.0
55	1606.0
56	1598.0
57	1590.0
58	1581.0
59	1572.0
60	1610.0
61	1648.0
62	1698.0
63	1811.0
64	1874.0
65	1891.5
66	1909.0
67	1822.0
68	1735.0
69	1752.0
70	1769.0
71	1530.0
72	1144.0
73	997.0
74	835.5
75	674.0
76	569.5
77	465.0
78	408.0
79	286.5
80	222.0
81	158.5
82	95.0
83	67.0
84	39.0
85	28.5
86	18.0
87	12.0
88	3.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.03280523339958704
10	0.0
11	0.001929719611740414
12	0.0
13	0.0
14	0.03280523339958704
15	0.15244784932749272
16	0.027016074564365795
17	0.00964859805870207
18	0.007718878446961656
19	0.00964859805870207
20	0.03473495301132746
21	0.007718878446961656
22	0.003859439223480828
23	0.0
24	0.0
25	0.0
26	0.20455027884448387
27	0.027016074564365795
28	0.19490168078578182
29	0.03280523339958704
30	0.04245383145828911
31	0.25279326913799427
32	0.00964859805870207
33	0.2084097180679647
34	0.10806429825746318
35	0.05210242951699118
36	0.1640261669979352
37	0.26823102603191756
38	0.1505181297157523
39	0.4110302773007082
40	0.6020725188630092
41	0.899249339071033
42	0.23542579263233052
43	0.3531386889484958
44	0.5364620520638351
45	0.11385345709268443
46	0.07332934524613573
47	0.027016074564365795
48	0.03859439223480828
49	0.33770093205457247
50	0.2450743906910326
51	0.3840142027363424
52	0.4399760714768144
53	0.19490168078578182
54	0.12736149437486732
55	0.1736747650566373
56	0.2026205592327435
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	51821.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.70616159472029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.74387964034541	81.28171976611799
2	4.168521321107451	7.228729665579592
3	0.9191667408528442	2.390922598946373
4	0.4117332858541796	1.4279925126879065
5	0.2292352888809757	0.9938056000463132
6	0.13798629039437374	0.7178556955674341
7	0.062316389210362325	0.37822504390112116
8	0.04451170657883023	0.30875513787846626
9	0.05341404789459628	0.4168194361359294
>10	0.20920502092050208	3.4233225912274947
>50	0.01335351197364907	0.8162713957661951
>100	0.006676755986824535	0.6155805561451921
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	112	0.21612859651492636	No Hit
CGCACCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC	104	0.2006908396210031	No Hit
CGCACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	103	0.19876112000926266	No Hit
CGCACCGGGGATGAGATGGACTTAAATGAATTGACGGGTCCGCACAGTTCTGAGTC	87	0.16788560622141602	No Hit
CGCACCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC	85	0.1640261669979352	No Hit
CGCACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	76	0.14665869049227145	No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	65	0.12543177476312692	No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	58	0.11192373748094402	No Hit
CGCACCGGGGATGAGATGGACTCAAATGAATTGACGGGTCCGCACAGTTCTGAGTC	52	0.10034541981050155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.005789158835221242	0.0	0.0	0.0	0.0
11	0.007718878446961656	0.0	0.0	0.0	0.0
12	0.017367476505663726	0.0	0.0	0.0	0.0
13	0.017367476505663726	0.0	0.0	0.0	0.0
14	0.021226915729144556	0.0	0.0	0.0	0.0
15	0.030875513787846625	0.0	0.0	0.0	0.0
16	0.03666467262306787	0.0	0.0	0.0	0.0
17	0.044383551070029524	0.0	0.0	0.0	0.0
18	0.050172709905250766	0.0	0.0	0.0	0.0
19	0.06175102757569325	0.0	0.0	0.0	0.0
20	0.0694699060226549	0.0	0.0	0.0	0.0
21	0.07525906485787615	0.0	0.0	0.0	0.0
22	0.07911850408135698	0.0	0.0	0.0	0.0
23	0.0829779433048378	0.0	0.0	0.0	0.0
24	0.09262654136353987	0.0	0.0	0.0	0.0
25	0.10420485903398236	0.0	0.0	0.0	0.0
26	0.11192373748094402	0.0	0.0	0.0	0.0
27	0.11771289631616526	0.0	0.0	0.0	0.0
28	0.12543177476312692	0.0	0.0	0.0	0.0
29	0.12736149437486732	0.0	0.0	0.0	0.0
30	0.13122093359834816	0.0	0.0	0.0	0.0
31	0.13122093359834816	0.0	0.0	0.0	0.0
32	0.13315065321008857	0.0	0.0	0.0	0.0
33	0.13315065321008857	0.0	0.0	0.0	0.0
34	0.13315065321008857	0.0	0.0	0.0	0.0
35	0.13315065321008857	0.0	0.0	0.0	0.0
36	0.13315065321008857	0.0	0.0	0.0	0.0
37	0.13315065321008857	0.0	0.0	0.0	0.0
38	0.13315065321008857	0.0	0.0	0.0	0.0
39	0.13315065321008857	0.0	0.0	0.0	0.0
40	0.13315065321008857	0.0	0.0	0.0	0.0
41	0.13315065321008857	0.0	0.0	0.0	0.0
42	0.14086953165705024	0.0	0.0	0.0	0.0
43	0.14665869049227148	0.0	0.0	0.0	0.0
44	0.15437756893923313	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCACC	15	0.008000013	50.598236	38
AACAACC	25	1.9193181E-5	50.598232	38
CAACCTG	20	3.928741E-4	50.548576	41
CAACCAC	20	3.928741E-4	50.548576	40
TACTTTT	15	0.008031167	50.548576	39
CTATCAA	15	0.008031167	50.548576	41
TACAAAC	20	3.9862574E-4	50.400196	37
AAACCCA	20	4.044441E-4	50.25268	36
TATGCTC	15	0.008251777	50.203705	35
CATCACT	15	0.008251777	50.203705	44
TCAGACT	15	0.008251777	50.203705	35
CACCACA	15	0.008251777	50.203705	44
GTGCGTA	25	2.0105088E-5	50.203705	44
GTGCGCA	15	0.008251777	50.203705	44
CGATTCA	15	0.008251777	50.203705	49
CCACACA	20	4.0639847E-4	50.2037	44
CTGCAAT	20	4.0639847E-4	50.2037	49
CCCACAC	15	0.008283657	50.15482	43
CCACCAC	15	0.008283657	50.15482	43
TCAAAAC	25	2.0221558E-5	50.15482	43
>>END_MODULE