FastQCFastQC Report
Fri 17 Jun 2016
SRR1526345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82969
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC3270.39412310622039554No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2420.29167520399185237No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2040.24587496534850367No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC1500.18079041569742915No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1370.16512191300365198No Hit
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1110.1337849076160976No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1040.12534802155021754No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1020.12293748267425184No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1020.12293748267425184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGA251.9384735E-550.55936440
TCGCCTA150.00821833650.28241342
TGTAGCG150.00821833650.28241342
CAACATA150.008277860550.19076544
ATTTTAT252.024351E-550.19076544
CGACTGT301.0091026E-650.19076544
ATGCGGG150.00829777350.16029446
GACTGTT355.1040843E-850.12985245
GTTGAAC150.00837777950.03876533
GTGTTAG150.00837777950.03876533
GTAGGAT204.1453083E-450.03876533
AGTGCGC150.00837777950.03876533
CATGTGC150.00837777950.03876550
ATTCGTG150.00845835849.91782814
TTTGCAC150.00845835849.91782813
ATTTCTA150.00845835849.91782814
CGTGGTA252.0908072E-549.91782414
GATACGC150.00847859249.887689
GAGTCAT150.00847859249.887689
AAGCCGT301.0522199E-649.8876810
Produced by FastQC (version 0.11.5)