##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526345_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82969 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.48016729139799 39.0 38.0 40.0 36.0 40.0 2 37.759825959093156 39.0 38.0 40.0 35.0 40.0 3 37.753425978377464 39.0 38.0 40.0 35.0 40.0 4 37.90315660065808 39.0 38.0 40.0 35.0 40.0 5 38.07811351227567 39.0 38.0 40.0 35.0 40.0 6 37.281237570658924 39.0 37.0 40.0 33.0 40.0 7 37.77506056478926 39.0 38.0 40.0 35.0 40.0 8 37.6260169460883 39.0 38.0 40.0 35.0 40.0 9 37.676312839735324 39.0 38.0 40.0 35.0 40.0 10 37.1558895491087 39.0 37.0 40.0 33.0 40.0 11 34.86210512360038 38.0 32.0 39.0 27.0 40.0 12 32.85012474538683 36.0 30.0 39.0 18.0 40.0 13 32.76727452421989 37.0 30.0 39.0 17.0 40.0 14 32.115440706769995 36.0 29.0 39.0 15.0 40.0 15 32.818884161554315 37.0 30.0 39.0 18.0 40.0 16 32.52290614566886 36.0 30.0 39.0 18.0 40.0 17 32.60017596933795 36.0 30.0 39.0 18.0 40.0 18 32.77818221263363 36.0 30.0 39.0 19.0 40.0 19 33.6009232363895 36.0 31.0 39.0 24.0 40.0 20 33.72781400282033 36.0 31.0 39.0 24.0 40.0 21 35.1937109040726 38.0 35.0 39.0 27.0 40.0 22 34.753474189154986 38.0 34.0 39.0 25.0 40.0 23 35.04976557509431 38.0 34.0 39.0 27.0 40.0 24 34.58521857561258 38.0 33.0 39.0 24.0 40.0 25 34.908953946654776 38.0 34.0 39.0 26.0 40.0 26 35.21411611565765 38.0 35.0 39.0 27.0 40.0 27 35.147838349262976 38.0 35.0 39.0 27.0 40.0 28 35.03520592028348 38.0 35.0 39.0 27.0 40.0 29 34.849365425640904 38.0 35.0 39.0 27.0 40.0 30 34.68507514854946 38.0 34.0 39.0 26.0 40.0 31 34.28650459810291 38.0 33.0 39.0 24.0 40.0 32 33.255914859766904 38.0 32.0 39.0 18.0 40.0 33 34.5004158179561 38.0 34.0 39.0 26.0 40.0 34 34.11274090322892 38.0 33.0 39.0 24.0 40.0 35 34.250756306572335 38.0 33.0 39.0 25.0 40.0 36 32.24949077366245 35.0 31.0 38.0 21.0 39.0 37 32.085200496571005 35.0 31.0 38.0 20.0 39.0 38 31.377851968807626 35.0 30.0 38.0 15.0 39.0 39 29.35242078366619 34.0 27.0 38.0 5.0 39.0 40 25.487615856524727 31.0 12.0 37.0 4.0 39.0 41 22.517265484699106 26.0 10.0 33.0 4.0 37.0 42 25.884486977063723 30.0 21.0 34.0 4.0 37.0 43 22.22916993093806 27.0 8.0 33.0 4.0 37.0 44 23.08016247032024 28.0 10.0 33.0 4.0 37.0 45 25.54490231291205 30.0 20.0 34.0 4.0 38.0 46 25.329448348178236 30.0 16.0 36.0 4.0 38.0 47 24.639600332654364 30.0 10.0 35.0 4.0 38.0 48 24.624799623955937 30.0 11.0 35.0 4.0 38.0 49 21.96556545215683 26.0 5.0 35.0 4.0 38.0 50 24.37830997119406 29.0 10.0 35.0 4.0 38.0 51 25.967240776675627 32.0 4.0 38.0 4.0 40.0 52 25.71079559835601 32.0 4.0 38.0 4.0 40.0 53 25.403246995865924 32.0 4.0 38.0 4.0 40.0 54 24.47886560040497 31.0 4.0 37.0 4.0 39.0 55 24.659402909520423 31.0 4.0 37.0 4.0 39.0 56 24.332883366076487 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.17687918252950396 1 2 0.3371156609679318 1 3 0.27786031711925574 1 4 0.40942915710265027 1 5 0.25418652376473716 1 6 0.9663206430829163 1 7 0.699156940055758 1 8 0.6733610583603209 1 9 0.7488753320564996 1 10 1.2452057736155098 1 11 -4.069406879738217 1 12 -7.276018135021019 1 13 -21.2205277973213 1 14 -17.499544746692692 1 15 -10.551653723980188 1 16 -21.082524422580594 1 17 -20.8191358547013 1 18 -19.76117905960266 1 19 -5.8410182167445654 1 20 -10.355826304936816 1 21 -8.080463410178929 1 22 -3.312997347534523 1 23 -4.086854191463473 1 24 -3.5462964834582245 1 25 -4.388920033865027 1 26 2.3365066697876387 1 27 2.46078453859716 1 28 2.0854087473593523 1 29 2.078827717182584 1 30 2.5372845734255876 1 31 1.9743122379757025 1 32 -12.72306867614888 1 33 0.8866719750272978 1 34 0.8781887047422074 1 35 1.113442223479403 1 36 3.1360332386604206 1 37 3.453592868407352 1 38 4.19717039768021 1 39 6.497516002446119 1 40 9.533028756794543 1 41 -2.914331001189833 1 42 -7.458315106044637 1 43 -13.957489948365659 1 44 -15.895798102570389 1 45 -14.108864805410052 1 46 -17.954092933744846 1 47 -17.550703191177696 1 48 -15.572082898195138 1 49 -13.612436484198899 1 50 -15.146202649774132 1 51 -16.638723284063467 1 52 -16.742069101774852 1 53 -16.881994574260837 1 54 -17.85510655224553 1 55 -16.711096635505196 1 56 -16.308817116182638 2 1 -0.19679676494708787 2 2 0.3199453312976033 2 3 0.5395361412950805 2 4 0.14019838787187666 2 5 0.057757952336160656 2 6 0.9505239397862155 2 7 0.5583602367590572 2 8 0.5991852341845032 2 9 0.4865126946938645 2 10 0.7953431362528747 2 11 -3.896329956661294 2 12 -13.482748904251789 2 13 -13.76379702809053 2 14 -15.436357933505878 2 15 -9.5887416360681 2 16 -4.3167277192838895 2 17 -3.1309490415144907 2 18 -10.929448290371894 2 19 -2.6603863486127004 2 20 -3.4032164148269253 2 21 2.141377249161728 2 22 2.6684586964215242 2 23 2.056689764580483 2 24 2.6920276923659543 2 25 2.1811349111899148 2 26 1.79461106539204 2 27 2.444301022113642 2 28 1.903403252853856 2 29 2.3446244204792848 2 30 2.336735122876135 2 31 -2.6980778719144105 2 32 -10.178425819006023 2 33 -2.4402511018957824 2 34 -1.949421185367683 2 35 -2.019799534762356 2 36 2.8214727990999755 2 37 2.484499461813943 2 38 3.805686881196692 2 39 6.711114903545017 2 40 8.526847438113222 2 41 5.332234932876101 2 42 2.0052837950542646 2 43 -0.9327646736403814 2 44 -10.226842058614343 2 45 0.9172340956888512 2 46 -12.653268757920673 2 47 -12.887241652716158 2 48 -9.629775205887446 2 49 -12.157079341341754 2 50 -9.22381253988402 2 51 -8.375673833514016 2 52 -8.331354816060568 2 53 -9.147791277557543 2 54 -15.013073585212565 2 55 -8.452854877263439 2 56 -7.91252590739143 3 1 0.37919832774647944 3 2 0.8863984392583504 3 3 0.9733556614695615 3 4 0.7590642548293474 3 5 0.6991156404495769 3 6 1.494212598455789 3 7 1.0253040753632021 3 8 1.1948777096479688 3 9 1.1485901210842684 3 10 0.9480830490118706 3 11 0.9158292581696728 3 12 1.6003345199575882 3 13 -4.44366344030427 3 14 -5.287230343538592 3 15 -3.5804264779437815 3 16 2.94465450536169 3 17 2.7312374361300016 3 18 2.7245184489956067 3 19 1.7741147418998438 3 20 1.4448615568197525 3 21 3.3705893538509315 3 22 3.706967420521849 3 23 3.136365337099569 3 24 3.4790369617225494 3 25 2.473255958081957 3 26 2.726658502687563 3 27 2.551716507391724 3 28 2.771928334642297 3 29 2.436431945015819 3 30 2.079101535089876 3 31 2.9887405577475334 3 32 2.1689705823025918 3 33 2.9362483528479473 3 34 2.896475761197202 3 35 2.2745025372114682 3 36 4.633223071728114 3 37 4.856159767157454 3 38 5.508385899735401 3 39 7.932966048583182 3 40 10.303769466466548 3 41 9.758011923061488 3 42 7.428607132022638 3 43 2.9748143885188334 3 44 2.343359686205723 3 45 6.808591783802264 3 46 0.07778358668129215 3 47 -4.026418315747783 3 48 0.07769480501767845 3 49 -9.12715840350097 3 50 0.2733930217299374 3 51 -0.4749786317691935 3 52 -0.11829592838335756 3 53 -0.7423250834463104 3 54 -4.673242614656402 3 55 -0.4861155097607117 3 56 2.4441398505147873 4 1 0.6899643187967541 4 2 1.3049822771104118 4 3 1.0685386043492713 4 4 1.2786220331920717 4 5 0.9733983464248297 4 6 1.9201667969290668 4 7 1.2431508771531412 4 8 1.4097763671894228 4 9 1.4415619557776083 4 10 1.8462667815730711 4 11 3.6960345753584107 4 12 3.724209223827028 4 13 5.500427109840508 4 14 4.653543544326638 4 15 6.134287920582143 4 16 6.73407277332695 4 17 6.0255780520815705 4 18 6.062238901746337 4 19 5.1916456710917345 4 20 5.067288511281447 4 21 3.418286116156807 4 22 3.861007957505265 4 23 3.3105690749253114 4 24 4.0108084805432895 4 25 3.315740822519963 4 26 2.658219439776275 4 27 2.713759150192459 4 28 2.616519016400659 4 29 2.580153977129534 4 30 2.955700640117513 4 31 3.5698408472974137 4 32 4.206980584934868 4 33 3.2550690951485564 4 34 2.7811822629081817 4 35 3.0340181992277877 4 36 4.473812700577813 4 37 5.040102910089804 4 38 5.318125304842013 4 39 7.633097662165703 4 40 9.570997684418643 4 41 6.686730006767725 4 42 8.098941430522245 4 43 8.738910203206906 4 44 11.531409172912753 4 45 9.448391731156832 4 46 9.29857853271972 4 47 10.105984948269064 4 48 9.226628734999252 4 49 4.839780264569576 4 50 8.919352484746522 4 51 5.9665059694416485 4 52 6.255960454875392 4 53 6.196974732294674 4 54 1.3198081881864496 4 55 5.97226827544592 4 56 5.832979018716944 5 1 0.6862238161667165 5 2 1.512287946309712 5 3 1.14925163766312 5 4 1.3629586784287753 5 5 1.1113293809075913 5 6 1.9337261189629658 5 7 1.6096616895435574 5 8 1.5368365659036272 5 9 1.564297198325825 5 10 1.8560271556233374 5 11 4.621224522757593 5 12 7.126663928677996 5 13 7.00633009055354 5 14 6.747056110077658 5 15 7.221488388144973 5 16 7.550554363040561 5 17 6.589692604974616 5 18 6.918463331904139 5 19 5.513796460104011 5 20 5.59481627282441 5 21 4.052242866595144 5 22 4.090522861070433 5 23 3.9793592561059086 5 24 4.529043430864739 5 25 3.9169097295918505 5 26 3.614677651348451 5 27 3.014518939789191 5 28 3.454099378761846 5 29 3.3063374955398217 5 30 3.4511419025371524 5 31 3.4164802394657343 5 32 4.602597183415284 5 33 3.693526137813663 5 34 4.098248306157743 5 35 3.2341350899349806 5 36 5.305431636872374 5 37 5.36903336011903 5 38 6.034197777080472 5 39 8.093530157782304 5 40 7.670763903004264 5 41 4.416829363868835 5 42 9.481524715151117 5 43 13.059366076964942 5 44 12.667528401434083 5 45 10.18690721917553 5 46 10.489285721498216 5 47 11.348533165685776 5 48 10.888989927284467 5 49 11.771340755510547 5 50 11.54237995406271 5 51 10.131438757285018 5 52 10.383838888539916 5 53 10.378389109851657 5 54 9.718463945053777 5 55 10.560462314019851 5 56 10.172371187039566 6 1 0.5241133733017591 6 2 0.9037586953529058 6 3 0.9660914408342549 6 4 0.9431937795769443 6 5 0.7242326067140397 6 6 1.6282313130581016 6 7 1.0914823565747227 6 8 1.0369732526176776 6 9 1.231328362896626 6 10 1.2495482053773017 6 11 4.524176955781101 6 12 6.410972293904894 6 13 5.798702971909471 6 14 6.549817181701499 6 15 6.566212280982594 6 16 6.446809147075555 6 17 6.232530221158321 6 18 6.0552867326695825 6 19 4.703380932493289 6 20 4.391426442315932 6 21 2.5765731016962903 6 22 3.439484042043645 6 23 2.5143510549030665 6 24 2.8334447430571927 6 25 2.9524482752452172 6 26 2.121511987051022 6 27 2.360199639625165 6 28 2.0805345892593863 6 29 2.3443364020461104 6 30 2.2480254454567827 6 31 1.8747907916800628 6 32 3.445134088828077 6 33 2.1933894510996126 6 34 2.5767884920516693 6 35 2.229624428371281 6 36 2.6699751032474417 6 37 2.546999461813943 6 38 3.1760785862658167 6 39 4.414743935803081 6 40 1.583011032583272 6 41 4.154527559604212 6 42 6.0729681268514994 6 43 10.705484174285886 6 44 8.90276623631653 6 45 5.849837782324794 6 46 8.48094783194108 6 47 8.488910421016563 6 48 8.478519724988132 6 49 10.392171810731977 6 50 8.298940916337177 6 51 7.791088839297046 6 52 7.973368686243582 6 53 8.264939137189003 6 54 9.048562359487896 6 55 8.362813325501538 6 56 7.8458266271707835 7 1 0.44120448818574687 7 2 1.0675957072374516 7 3 0.9604321312700179 7 4 1.1000981372453111 7 5 1.041153942311098 7 6 1.3394650425431038 7 7 1.435709860819209 7 8 1.337907038695775 7 9 1.2882044240171737 7 10 1.76949727159122 7 11 4.431364278927674 7 12 6.73921224863042 7 13 6.79431700699719 7 14 7.688328783286103 7 15 7.012448840122378 7 16 7.090697092746183 7 17 7.006582286806314 7 18 6.7812346670215895 7 19 5.221349240359736 7 20 4.821251003719439 7 21 3.090155444650456 7 22 4.451510074867635 7 23 3.3077862558085513 7 24 4.214740724947404 7 25 3.616285287129763 7 26 3.1331135716577023 7 27 3.114727087276549 7 28 3.3477954834303034 7 29 3.495157001932924 7 30 2.709542140419998 7 31 3.151389546631954 7 32 4.1729212987884665 7 33 3.2871925071267754 7 34 3.382815656737577 7 35 3.0882831720045587 7 36 1.8305580121325598 7 37 1.9943678828665732 7 38 1.9576292370864152 7 39 4.512649991264318 7 40 -1.6691112085033168 7 41 -10.49530266110886 7 42 4.397671013099377 7 43 9.035280439141573 7 44 8.529110322338038 7 45 5.28220903303221 7 46 7.983636003984088 7 47 8.582996442521939 7 48 8.502430307897015 7 49 11.329919405525413 7 50 8.186745104226254 7 51 9.904557996059921 7 52 9.804296813012112 7 53 10.709194937981305 7 54 10.303655738095706 7 55 10.1774709372729 7 56 9.958871335716339 8 1 0.7963933076921421 8 2 0.8258472683436082 8 3 1.4204380783410855 8 4 1.0748230852084362 8 5 0.9757361605686086 8 6 1.6116922206578863 8 7 1.07576338445881 8 8 0.9520908031917656 8 9 1.233788723749555 8 10 1.7119593590131643 8 11 4.417834692249116 8 12 6.592765623593248 8 13 6.692770768519637 8 14 6.967395093128502 8 15 6.4248782186534505 8 16 6.813266227447343 8 17 6.403251927008512 8 18 5.969310789531253 8 19 5.3104066295955334 8 20 5.577867120282036 8 21 3.247158120832431 8 22 3.6583194712399276 8 23 3.3607151883092996 8 24 3.435823091881687 8 25 3.1965707465410063 8 26 3.0468810411789633 8 27 3.107739278772236 8 28 2.8947773448635417 8 29 3.6283713938449083 8 30 3.798599529655796 8 31 3.865632781838613 8 32 5.119546335957658 8 33 4.108780375101794 8 34 4.521977119717064 8 35 2.8697283102739632 8 36 1.4579740097537304 8 37 1.6063214957122476 8 38 1.873180827927925 8 39 3.602004734053491 8 40 -1.0072021986906492 8 41 -10.108594364944722 8 42 5.566270477862979 8 43 9.694959297303928 8 44 8.099731791264592 8 45 6.076737727650109 8 46 8.82826877234567 8 47 9.484126386024766 8 48 9.126278062877688 8 49 11.169645840256308 8 50 9.610176564232201 8 51 9.538218418301966 8 52 9.256720244472124 8 53 9.437711143749961 8 54 9.947277504375812 8 55 9.68758095808765 8 56 8.757116949751428 9 1 0.6166430846769728 9 2 0.8921571107462256 9 3 0.7499058154805454 9 4 1.1088700670698728 9 5 1.1288732405567146 9 6 1.6464825864027546 9 7 1.2690431941525375 9 8 1.039661424660693 9 9 1.2355728450698038 9 10 1.4361639382578915 9 11 4.045399366646976 9 12 6.546229792490063 9 13 5.9785275333129775 9 14 7.390083169251014 9 15 6.065080419069741 9 16 7.002977794500573 9 17 6.269740181543156 9 18 5.904041684565453 9 19 4.993279064921133 9 20 5.3387948633685625 9 21 3.2305063218434356 9 22 4.021685513464128 9 23 3.1762073084401337 9 24 4.065617917929863 9 25 3.721548445024503 9 26 2.4576749751664764 9 27 1.8778849820133914 9 28 2.286391974658372 9 29 2.091648230003095 9 30 2.762173719367361 9 31 2.870687792245988 9 32 4.067658140893727 9 33 2.9363153141443163 9 34 3.11088583217618 9 35 2.5181077334080655 9 36 1.2516106437115049 9 37 1.3277012161999089 9 38 2.2558748511215043 9 39 3.5477377105625614 9 40 -1.5463041909594573 9 41 -2.793548275143948 9 42 5.906442942923938 9 43 9.886157632124029 9 44 8.222092778478387 9 45 5.720805524260278 9 46 8.501179863633212 9 47 9.697031530241237 9 48 8.669096974563683 9 49 11.356235194999098 9 50 8.511306507735025 9 51 8.49227729430553 9 52 7.918331900731413 9 53 8.595159850262007 9 54 8.45277854511325 9 55 8.30027795481676 9 56 7.151853791856691 10 1 0.42853392288360226 10 2 1.0934241672764387 10 3 0.6880149772739159 10 4 0.7341137317872182 10 5 0.6668849364631484 10 6 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4.609816762087231 115 43 5.715037524161815 115 44 1.6778282710559864 115 45 4.728497831952588 115 46 0.31184113218921894 115 47 -4.445208685683191 115 48 -3.9632916894039276 115 49 -5.559689231451644 115 50 0.6164347128632315 115 51 0.6206173752771953 115 52 0.2777111180053673 115 53 0.16196146969520342 115 54 0.27895938182040325 115 55 1.1834088590002985 115 56 3.9878861805206576 116 1 0.0329110272607096 116 2 0.7845016083538852 116 3 0.7427287819877222 116 4 0.4756962233697166 116 5 0.3082990778772867 116 6 1.0898637666260385 116 7 0.8097130506118688 116 8 0.827540212539482 116 9 0.47082005400122284 116 10 1.32212885053859 116 11 -1.035940346271687 116 12 -1.2249025839054681 116 13 -7.587660664454166 116 14 -8.950427197575141 116 15 -5.123914796241262 116 16 -9.593513433569605 116 17 -11.700754236319685 116 18 -4.996277294700896 116 19 -5.871154746881096 116 20 -5.335846284956794 116 21 1.1762798465643272 116 22 1.3932963587591871 116 23 1.581310976701694 116 24 2.1533372161754727 116 25 1.9392517943067986 116 26 1.8165266498076207 116 27 2.279798857611482 116 28 2.4307300926806974 116 29 2.561345199700064 116 30 2.056432092573104 116 31 0.4942922579557205 116 32 -6.666791619871823 116 33 1.5694891578444796 116 34 1.6850485116020124 116 35 2.0803086903458663 116 36 2.4756935783207545 116 37 2.7894237042381853 116 38 3.5118557123655236 116 39 6.686493691423806 116 40 8.645083368849157 116 41 6.526228439369607 116 42 3.113779466049934 116 43 -10.845102335978046 116 44 -0.4557381625104462 116 45 1.5844418878966415 116 46 -5.304242783894697 116 47 -8.841245981720487 116 48 -7.533454859567099 116 49 -6.170878042640457 116 50 -1.9735419771134595 116 51 0.991479846139665 116 52 1.5826062229004734 116 53 0.4579987657325013 116 54 -3.7776839748229527 116 55 0.7332923088837475 116 56 3.6359048285393065 117 1 -0.6073210810565115 117 2 0.15942101518817964 117 3 0.08379905922821251 117 4 0.056611761732057175 117 5 -0.3142025339860268 117 6 0.43931573309928496 117 7 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1.1413453570890155 118 28 1.2682431543316923 118 29 1.3387746667847082 118 30 0.9660454677037222 118 31 -1.5887798423577593 118 32 -7.3757780357547915 118 33 -0.6656205600238607 118 34 -1.373990150884925 118 35 -1.5832231800825554 118 36 1.4945023557502246 118 37 -0.05644881404812452 118 38 1.5526080634626993 118 39 3.3813735242346716 118 40 5.460652856832436 118 41 4.076385179181521 118 42 -0.3872654660294792 118 43 -1.2024691071379188 118 44 -0.7582459995010424 118 45 -1.158504820567309 118 46 -5.056593881073383 118 47 -10.256084017248178 118 48 -10.214750575345574 118 49 -7.363668011292493 118 50 -5.97719923939141 118 51 -4.730045754696281 118 52 -5.392315407193571 118 53 -6.471990784946705 118 54 -9.11467457043424 118 55 -7.213938621105804 118 56 -3.708400291627882 119 1 -0.5985824792328032 119 2 0.007445331297603275 119 3 0.06632185558079584 119 4 0.015198387871876662 119 5 -0.22795633337812404 119 6 0.12016679692906962 119 7 -0.5934254775266581 119 8 -0.4365290515297886 119 9 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-6.547955938430444 119 48 -9.183346634458873 119 49 -5.93386505562747 119 50 -4.7148839684554495 119 51 -7.116745262085445 119 52 -7.206354816060568 119 53 -9.281719848986114 119 54 -7.941645013783994 119 55 -9.14928344869201 119 56 -5.296454478819999 120 1 -0.7342341083054791 120 2 -0.4938078015344729 120 3 -0.48693628978261216 120 4 -0.4876211610003054 120 5 -0.5728811454081963 120 6 -0.16053495745689617 120 7 -0.6256936479527226 120 8 -0.38139120691825923 120 9 -0.9135499619477443 120 10 0.32212885053859 120 11 -11.051091861423199 120 12 -3.1730684531239675 120 13 -6.819718080722108 120 14 -7.408162444784072 120 15 -9.391059931807447 120 16 -9.163688872166095 120 17 -10.853066836000707 120 18 -2.3328004206977084 120 19 -3.436545496482374 120 20 -3.854187592771787 120 21 0.546295795527648 120 22 0.6708083204816759 120 23 -0.043090937173900556 120 24 -5.250171555754349 120 25 0.019794059059591973 120 26 2.519078483938401 120 27 2.290165683767775 120 28 1.856567413254865 120 29 2.819718405441691 120 30 2.1569105614726283 120 31 -0.3836981726662927 120 32 -10.941113788930835 120 33 -2.282982931469718 120 34 -7.4505176765957515 120 35 -0.7099624420305304 120 36 0.17266327529045356 120 37 0.5908591109367514 120 38 1.2295590616478194 120 39 3.6091412193344894 120 40 1.6905379143037003 120 41 3.3906622511718396 120 42 -1.9707500395322022 120 43 -0.5348949994549201 120 44 -2.716503712749681 120 45 -8.182703247669547 120 46 -11.998820136366788 120 47 -11.302968469758763 120 48 -9.085288990348598 120 49 -14.582361296228973 120 50 -8.374658404545675 120 51 -8.65684551271201 120 52 -8.125527748391395 120 53 -9.31712085149238 120 54 -10.47704601629026 120 55 -9.471651869744642 120 56 -5.295514629195942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 154.0 5 39.0 6 127.0 7 77.0 8 167.0 9 345.0 10 219.0 11 233.0 12 229.0 13 174.0 14 226.0 15 288.0 16 346.0 17 490.0 18 667.0 19 814.0 20 1109.0 21 1275.0 22 1488.0 23 1972.0 24 2281.0 25 2461.0 26 2586.0 27 2722.0 28 2904.0 29 3115.0 30 3423.0 31 3970.0 32 4746.0 33 5687.0 34 6610.0 35 7709.0 36 8669.0 37 8613.0 38 5215.0 39 1818.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.50222372211309 0.3712229868987212 0.09039520784871458 0.03615808313948583 8 98.8959731948077 0.8376622593980884 0.1337849076160976 0.13257963817811472 9 94.70778281415758 2.9149387597646834 1.3706721959687531 1.0066062301089787 10 55.70875869300582 28.87222938687944 6.9049886102038105 8.51402330991093 11 40.275300130176944 26.38493804541729 17.113446796200762 16.226315028205004 12 38.73977027564512 14.488543913991972 20.09545733948824 26.676228470874662 13 25.564970049054462 14.791066542925671 23.669081223107426 35.97488218491244 14 22.317725913781462 16.650593114090075 28.95650496672775 32.075176005400714 15 27.636407532592948 17.8887011105746 25.353693867696762 29.12119748913568 16 32.76022136751106 20.343867179493362 22.25008741364135 24.645824039354224 17 29.52153629163502 23.707400576229915 23.349366508745916 23.42169662338915 18 31.547733847637417 26.9202025072324 20.291706846673097 21.24035679845709 19 27.88432561447497 32.549393059054694 19.354605397977267 20.211675928493076 20 30.281257158012753 30.65136409118856 18.66327502441259 20.40410372638609 21 26.4588531684326 30.59824732699285 22.03377572053665 20.9091237840379 22 25.764771111057545 26.672371814960343 25.894944917194994 21.667912156787118 23 29.243452373778155 26.964287866552567 23.59194397907652 20.20031578059275 24 28.875845195193385 27.19811013752124 21.04159384830479 22.884450818980582 25 27.17674284061325 27.868575836467073 19.554527046572172 25.400154276347507 26 22.473131264340054 26.360342953749548 26.200941915227627 24.965583866682767 27 21.83883101851852 25.066309799382715 24.952980324074073 28.141878858024693 28 21.71136037091146 31.456238001521314 23.212151214034748 23.620250413532474 29 26.9841461209235 28.62981493760926 23.854361323768764 20.531677617698474 30 24.777566127359968 26.267692233501315 28.488414148964385 20.46632749017433 31 27.140941543145004 26.216794114449314 27.49610408436922 19.14616025803646 32 29.737939680320164 23.327466910966997 25.35861520287374 21.5759782058391 33 29.63195517774343 25.48541344667697 27.21454791344668 17.668083462132923 34 22.007382032230048 26.65251375084435 34.725706841648176 16.614397375277427 35 21.359586191928813 33.564030529196856 29.488648009935254 15.587735268939074 36 22.699727178348102 33.85040681812695 26.87293271204037 16.576933291484586 37 25.02960152719716 32.06389096972187 25.685668027934177 17.2208394751468 38 23.753439204518028 33.16599893807018 26.350340300236518 16.730221557175266 39 23.128305037573057 24.32023620808577 23.787799949176538 28.76365880516463 40 15.521696407991945 17.540305460191913 20.158189074763747 46.7798090570524 41 15.181482022089233 22.19384031528244 22.185325743200508 40.43935191942782 42 18.3008114374034 28.57539605873261 29.071676970633693 24.052115533230296 43 12.33430091920658 15.335026608611516 23.120464441219156 49.21020803096275 44 14.494456897073968 17.323559701944628 21.336402738232266 46.845580662749136 45 22.669417399592216 28.02610783355653 28.081605076791295 21.222869690059962 46 19.531080396555467 30.140868852063583 28.121909448344066 22.20614130303688 47 17.32577767060526 25.408729201832653 28.952254641909814 28.31323848565228 48 21.02731054440224 28.35111834569241 32.61952131187074 18.002049798034605 49 16.36469193739568 17.735068579307676 21.328527540579113 44.57171194271753 50 19.236018018670823 27.759863773051702 30.75854739562576 22.245570812651717 51 18.9365355778068 28.525129395830312 32.00067721182218 20.537657814540704 52 18.123964042442502 28.649897765356368 31.39389979795047 21.832238394250663 53 18.874987923872087 26.584387981837505 34.34088493865327 20.199739155637136 54 16.435975720129846 23.4496240964437 30.408968588219675 29.705431595206775 55 19.84186383389667 26.638097537421533 30.18469338483824 23.335345243843552 56 19.91933243971066 28.18654977116014 28.250552476180125 23.643565312949075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 1.0 11 1.0 12 13.5 13 118.0 14 210.0 15 336.0 16 462.0 17 613.5 18 765.0 19 869.0 20 973.0 21 967.0 22 973.5 23 986.0 24 1024.0 25 1062.0 26 1125.5 27 1189.0 28 1311.5 29 1623.0 30 1812.0 31 1920.5 32 2029.0 33 2070.0 34 2111.0 35 2026.0 36 1941.0 37 1857.5 38 1613.5 39 1453.0 40 1410.0 41 1367.0 42 1356.0 43 1345.0 44 1381.0 45 1417.0 46 1447.5 47 1602.0 48 1726.0 49 1904.0 50 2082.0 51 2216.5 52 2351.0 53 2458.5 54 2819.5 55 3073.0 56 3341.0 57 3609.0 58 4021.0 59 4433.0 60 4923.5 61 5414.0 62 6046.5 63 6736.0 64 6793.0 65 6176.0 66 5559.0 67 4841.5 68 4124.0 69 3605.0 70 3086.0 71 2704.5 72 1988.5 73 1654.0 74 1401.0 75 1148.0 76 950.5 77 753.0 78 604.5 79 324.0 80 192.0 81 140.0 82 88.0 83 64.0 84 40.0 85 27.5 86 15.0 87 9.0 88 3.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02048958044570864 10 0.0 11 0.006026347189914305 12 0.0 13 0.0 14 0.02048958044570864 15 0.15547975749978907 16 0.03615808313948583 17 0.019284311007725777 18 0.010847424941845749 19 0.014463233255794333 20 0.02410538875965722 21 0.01205269437982861 22 0.003615808313948583 23 0.0 24 0.0 25 0.001205269437982861 26 0.19163784063927491 27 0.030131735949571525 28 0.1759693379454977 29 0.028926466511588665 30 0.0277211970736058 31 0.2290011932167436 32 0.013257963817811472 33 0.18440622401137774 34 0.07834251346888597 35 0.038568622015451554 36 0.1578902963757548 37 0.2446696959105208 38 0.12173221323626897 39 0.3977389145343442 40 0.6460244187588136 41 0.9123889645530259 42 0.18440622401137774 43 0.34832286757704684 44 0.5230869360845617 45 0.09883209391459462 46 0.06628981908905736 47 0.034952813701502966 48 0.04097916089141728 49 0.35073340645301254 50 0.20007472670515494 51 0.33385963432125254 52 0.3808651424025841 53 0.19404837951524065 54 0.12293748267425184 55 0.15547975749978907 56 0.19284311007725777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 82969.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.7051308319975 #Duplication Level Percentage of deduplicated Percentage of total 1 92.88123052405413 78.67516783376925 2 4.5803155992544005 7.75952464173366 3 1.181007128729777 3.001120900577324 4 0.486631853042872 1.6488085911605541 5 0.25612202791730104 1.0847424941845751 6 0.12521521364845828 0.6363822632549506 7 0.10387171132201654 0.615892682809242 8 0.05549310604874856 0.37604406465065265 9 0.0668763072895175 0.5098289722667502 >10 0.24189302636633986 3.443454784317034 >50 0.008537400930576701 0.46643927249936723 >100 0.012806101395865051 1.7825934987766516 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 327 0.39412310622039554 No Hit CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 242 0.29167520399185237 No Hit CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 204 0.24587496534850367 No Hit CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC 150 0.18079041569742915 No Hit CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 137 0.16512191300365198 No Hit CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 111 0.1337849076160976 No Hit CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 104 0.12534802155021754 No Hit CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 102 0.12293748267425184 No Hit CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 102 0.12293748267425184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.002410538875965722 0.0 0.0 0.0 0.0 10 0.006026347189914305 0.0 0.0 0.0 0.0 11 0.013257963817811472 0.0 0.0 0.0 0.0 12 0.014463233255794333 0.0 0.0 0.0 0.0 13 0.016873772131760056 0.0 0.0 0.0 0.0 14 0.016873772131760056 0.0 0.0 0.0 0.0 15 0.020489580445708637 0.0 0.0 0.0 0.0 16 0.02290011932167436 0.0 0.0 0.0 0.0 17 0.02290011932167436 0.0 0.0 0.0 0.0 18 0.02410538875965722 0.0 0.0 0.0 0.0 19 0.03374754426352011 0.0 0.0 0.0 0.0 20 0.03495281370150297 0.0 0.0 0.0 0.0 21 0.038568622015451554 0.0 0.0 0.0 0.0 22 0.04459496920536586 0.0 0.0 0.0 0.0 23 0.04700550808133158 0.0 0.0 0.0 0.0 24 0.04821077751931444 0.0 0.0 0.0 0.001205269437982861 25 0.05544239414721161 0.0 0.0 0.0 0.001205269437982861 26 0.06628981908905736 0.0 0.0 0.0 0.001205269437982861 27 0.07231616627897167 0.0 0.0 0.0 0.001205269437982861 28 0.07593197459292025 0.0 0.0 0.0 0.001205269437982861 29 0.07713724403090311 0.0 0.0 0.0 0.001205269437982861 30 0.07713724403090311 0.0 0.0 0.0 0.001205269437982861 31 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 32 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 33 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 34 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 35 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 36 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 37 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 38 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 39 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 40 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 41 0.07954778290686883 0.0 0.0 0.0 0.001205269437982861 42 0.0807530523448517 0.0 0.0 0.0 0.001205269437982861 43 0.08316359122081741 0.0 0.0 0.0 0.001205269437982861 44 0.09039520784871458 0.0 0.0 0.0 0.001205269437982861 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCCGA 25 1.9384735E-5 50.559364 40 TCGCCTA 15 0.008218336 50.282413 42 TGTAGCG 15 0.008218336 50.282413 42 CAACATA 15 0.0082778605 50.190765 44 ATTTTAT 25 2.024351E-5 50.190765 44 CGACTGT 30 1.0091026E-6 50.190765 44 ATGCGGG 15 0.008297773 50.160294 46 GACTGTT 35 5.1040843E-8 50.129852 45 GTTGAAC 15 0.008377779 50.038765 33 GTGTTAG 15 0.008377779 50.038765 33 GTAGGAT 20 4.1453083E-4 50.038765 33 AGTGCGC 15 0.008377779 50.038765 33 CATGTGC 15 0.008377779 50.038765 50 ATTCGTG 15 0.008458358 49.917828 14 TTTGCAC 15 0.008458358 49.917828 13 ATTTCTA 15 0.008458358 49.917828 14 CGTGGTA 25 2.0908072E-5 49.917824 14 GATACGC 15 0.008478592 49.88768 9 GAGTCAT 15 0.008478592 49.88768 9 AAGCCGT 30 1.0522199E-6 49.88768 10 >>END_MODULE