FastQCFastQC Report
Fri 17 Jun 2016
SRR1526344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25284
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC790.31245056161999685No Hit
GACACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC310.12260718240784686No Hit
GACACTGGGGCCGCATAGCCTCTCCACCCAAGCGGCCGGAGACCCTGCCTGCAATC270.10678690080683437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACCA150.00796001350.58551439
CGACCCT150.00796001350.58551440
GAGACCC203.8755895E-450.5855139
GATACCC150.00808776950.38276735
AGAGCGC150.00808776950.38276735
ATACCCA150.00808776950.38276736
ATAGGAC150.00815221750.28234
TGATACC150.00815221750.28234
TAGAGCG150.00815221750.28234
ATTCTGA150.00815221750.28234
CGCTGCA150.00821704550.1816444
CCCAGCC150.00821704550.1816443
CCAGCCC150.00821704550.1816444
GTCTTCA150.00821704550.1816450
AAATGGT150.00821704550.1816450
CTTCTCT309.821597E-750.1816444
TGAGTCG204.0325962E-450.18163350
CAGCCCC150.00828225650.08167645
CAGTCTT150.00828225650.08167648
GAAGAGC150.00828225650.08167633