##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526344_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25284 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.24675684227179 39.0 38.0 39.0 33.0 40.0 2 35.961161208669516 39.0 36.0 39.0 28.0 40.0 3 36.718043031165955 39.0 38.0 39.0 30.0 40.0 4 34.68940832146812 39.0 35.0 39.0 18.0 40.0 5 36.65088593576966 39.0 37.0 39.0 30.0 40.0 6 36.68232874545167 39.0 38.0 39.0 31.0 40.0 7 36.813676633444075 39.0 37.0 39.0 31.0 40.0 8 36.53504192374624 39.0 36.0 39.0 30.0 40.0 9 36.43058851447556 38.0 36.0 39.0 30.0 40.0 10 36.29053947160259 38.0 36.0 39.0 30.0 40.0 11 35.394716025945264 38.0 34.0 39.0 29.0 40.0 12 33.42133364973896 38.0 31.0 39.0 20.0 40.0 13 32.72223540579022 37.0 30.0 39.0 17.0 40.0 14 32.45732479038127 37.0 29.0 39.0 16.0 40.0 15 32.80339345040342 37.0 30.0 39.0 18.0 40.0 16 32.40404999208986 36.0 30.0 39.0 18.0 40.0 17 32.29251700680272 36.0 29.0 39.0 17.0 40.0 18 32.44205821863629 36.0 30.0 38.0 18.0 40.0 19 33.40278436956178 36.0 31.0 38.0 23.0 40.0 20 33.742248062015506 37.0 31.0 39.0 24.0 40.0 21 35.128223382376206 38.0 35.0 39.0 27.0 40.0 22 34.83622053472552 38.0 34.0 39.0 25.0 40.0 23 35.06450719822813 38.0 35.0 39.0 27.0 40.0 24 34.70079892422085 38.0 34.0 39.0 25.0 40.0 25 34.94656699889258 38.0 34.0 39.0 27.0 40.0 26 35.37173706691979 38.0 35.0 39.0 28.0 40.0 27 35.36940357538364 38.0 35.0 39.0 28.0 40.0 28 35.389020724568894 38.0 35.0 39.0 29.0 40.0 29 35.140642303433005 38.0 35.0 39.0 27.0 40.0 30 35.01487106470495 38.0 35.0 39.0 27.0 40.0 31 34.92568422717925 38.0 35.0 39.0 27.0 40.0 32 33.82225913621262 38.0 33.0 39.0 20.0 40.0 33 35.124307862679956 38.0 35.0 39.0 28.0 40.0 34 34.92857142857143 38.0 35.0 39.0 27.0 40.0 35 34.88202024996045 38.0 35.0 39.0 27.0 40.0 36 32.866318620471446 36.0 31.0 38.0 24.0 39.0 37 32.651479196329696 35.0 31.0 38.0 23.0 39.0 38 31.9187628539788 35.0 31.0 38.0 18.0 39.0 39 29.896218952697357 35.0 28.0 38.0 8.0 39.0 40 25.84246954595792 32.0 13.0 38.0 4.0 39.0 41 22.276894478721722 26.0 10.0 33.0 4.0 37.0 42 26.073168802404684 30.0 22.0 34.0 4.0 37.0 43 22.2078389495333 27.0 9.0 33.0 4.0 37.0 44 23.275866160417657 28.0 10.0 33.0 4.0 37.0 45 25.934583135579814 30.0 22.0 34.0 4.0 38.0 46 26.081316247429204 31.0 19.0 36.0 4.0 38.0 47 25.32799398829299 30.0 13.0 36.0 4.0 38.0 48 25.241061540895426 30.0 15.0 36.0 4.0 38.0 49 22.44332384116437 27.0 5.0 35.0 4.0 38.0 50 25.08408479670938 30.0 14.0 35.0 4.0 38.0 51 26.779307071665876 33.0 11.0 38.0 4.0 40.0 52 26.53769182091441 33.0 9.0 38.0 4.0 40.0 53 26.094447081158044 32.0 4.0 38.0 4.0 40.0 54 25.18529504825186 32.0 4.0 38.0 4.0 39.0 55 25.505220692928333 32.0 4.0 38.0 4.0 39.0 56 24.521476032273373 32.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.7195266948076338 1 2 3.341560273699244 1 3 2.4589281456659435 1 4 4.961175339669509 1 5 2.5063672101814802 1 6 2.4457062911269603 1 7 2.5797659101471524 1 8 2.644991795050508 1 9 2.9367304755776686 1 10 2.6469111431475127 1 11 -4.150920921609934 1 12 -7.189281896972243 1 13 -22.172136102640206 1 14 -18.834954131403457 1 15 -10.482146142523963 1 16 -20.185971655201758 1 17 -20.08557308586033 1 18 -19.756433963288938 1 19 -5.5279210211096235 1 20 -10.317500547949042 1 21 -8.43119864288596 1 22 -2.566680165581115 1 23 -2.3210282199840186 1 24 -3.408108687754833 1 25 -1.453051966925429 1 26 2.878747832847047 1 27 2.926625620388471 1 28 3.0127854485452588 1 29 3.143733949613562 1 30 2.5547513735935183 1 31 2.0643930205978975 1 32 -11.410901691999705 1 33 0.7175437353933205 1 34 1.6242805326576715 1 35 1.864166946902749 1 36 5.469121853626593 1 37 5.4689644339544685 1 38 6.8655970459038045 1 39 8.492843002849941 1 40 10.48602170897237 1 41 -3.9580500715104243 1 42 -9.689733536115739 1 43 -13.014629228250676 1 44 -16.107621887249973 1 45 -13.718690303174046 1 46 -17.645609298749537 1 47 -17.720793012995188 1 48 -16.301063620847938 1 49 -13.92035199124831 1 50 -15.589660677350686 1 51 -17.23239492837814 1 52 -16.75159250214574 1 53 -16.351202138740625 1 54 -18.238101890868297 1 55 -16.474274664000898 1 56 -15.478444931801796 2 1 1.5093973844628081 2 2 2.8437154461130376 2 3 2.242333318079737 2 4 4.951477063807438 2 5 2.352272382595274 2 6 2.260361463540754 2 7 2.425671082560946 2 8 2.798009036429818 2 9 2.7233684066121526 2 10 2.536997350044068 2 11 -2.4235502319547635 2 12 -8.660187069386037 2 13 -13.466317137122964 2 14 -13.289695510713802 2 15 -8.768784073558447 2 16 -2.1838164827879645 2 17 -1.3679006720672255 2 18 -9.475183963288938 2 19 -0.26391240041996866 2 20 -2.202198823811109 2 21 2.882378943320937 2 22 3.264138799936127 2 23 2.6391010903608105 2 24 3.4636585536244766 2 25 2.980137688246984 2 26 2.586721970778079 2 27 2.6690825169401933 2 28 2.7573975175107748 2 29 3.5833891220273557 2 30 2.7713462011797247 2 31 -1.2858224966434832 2 32 -9.87426376096522 2 33 -0.5572407473652987 2 34 -0.7183918811354317 2 35 -0.2004882255110445 2 36 5.312871853626593 2 37 5.51314546843723 2 38 6.143614287283114 2 39 8.710515416643045 2 40 10.415978605524092 2 41 7.665872342282679 2 42 4.1884992225049515 2 43 -2.122387848940331 2 44 -8.728311542422386 2 45 2.829801076136299 2 46 -9.64129895392195 2 47 -7.62057749575381 2 48 -5.524123965675525 2 49 -10.016257163662104 2 50 -5.545479642867928 2 51 -3.128946652516074 2 52 -3.105040778007808 2 53 -2.6701676559820022 2 54 -13.157282925351055 2 55 -3.069102250207795 2 56 -3.3642207938707607 3 1 1.8829092892247132 3 2 3.1740725889701835 3 3 2.434297603794022 3 4 5.381536587616964 3 5 2.505546192119084 3 6 2.540123368302659 3 7 2.9837067968466613 3 8 3.229556655477438 3 9 3.279916025659773 3 10 2.522116397663119 3 11 1.3934140537595212 3 12 1.573443882994912 3 13 -5.833876660932489 3 14 -6.185528844047134 3 15 -2.7777126449870195 3 16 2.2893978029263202 3 17 2.3791231374565847 3 18 2.9102327033777264 3 19 1.288170932913367 3 20 1.3171464142841316 3 21 3.3898194195114115 3 22 3.485864990412317 3 23 3.3727320427417595 3 24 4.247884744100666 3 25 4.072399593008889 3 26 3.382852923159028 3 27 3.4652134693211423 3 28 2.2172189460822054 3 29 3.547674836313071 3 30 3.07938191546544 3 31 3.257332265261276 3 32 1.8370457628443049 3 33 2.715080681206132 3 34 2.7786319283883785 3 35 2.507845107822291 3 36 5.969121853626593 3 37 5.083085944627705 3 38 6.97397143014026 3 39 8.457539226166855 3 40 11.000800034095523 3 41 10.078074723235058 3 42 7.4161777939335245 3 43 2.3835645320120484 3 44 4.033593219482377 3 45 7.465217742802967 3 46 0.7813200936970972 3 47 -2.770875114801431 3 48 2.200578415276855 3 49 -9.371911925566867 3 50 1.6911274999892143 3 51 -0.10513712870654857 3 52 -0.1407550637220929 3 53 -0.20588194169628693 3 54 -7.804604353922484 3 55 -1.9619593930649373 3 56 2.0732792061292393 4 1 1.8559251622405881 4 2 3.0312154461130376 4 3 2.6374722069686243 4 4 5.102171508251885 4 5 2.4849112714841652 4 6 2.757583685762974 4 7 2.447198860338723 4 8 2.6993979253187064 4 9 2.949757295501044 4 10 2.718941794488515 4 11 3.207699768045238 4 12 2.943285152836186 4 13 5.867710640654813 4 14 0.5811378226195316 4 15 6.482604815330443 4 16 7.014794628323148 4 17 6.747377105710552 4 18 5.421343814488839 4 19 4.777059821802254 4 20 4.779051176188894 4 21 3.9580733877653813 4 22 3.80649991104724 4 23 3.8759066459163662 4 24 4.046297442513364 4 25 3.6120821326914268 4 26 3.2082497485558576 4 27 2.6906102947179704 4 28 3.0442030730663276 4 29 3.2333891220273543 4 30 2.8650962011797247 4 31 2.0176497255787424 4 32 2.402125127923668 4 33 2.297620363745814 4 34 2.369108118864567 4 35 2.752289552266731 4 36 5.424677409182149 4 37 5.651339912881674 4 38 6.342225398394227 4 39 8.992459861087486 4 40 10.116673049968536 4 41 8.897122342282678 4 42 9.966971444727172 4 43 8.680389928837446 4 44 12.360577346466503 4 45 10.354106631691856 4 46 9.778145490522494 4 47 9.881505837579521 4 48 9.764070478768918 4 49 8.034437280782342 4 50 9.859381468243182 4 51 9.29327556970615 4 52 9.54495922199219 4 53 8.49094345512911 4 54 2.3573004079822795 4 55 9.114231083125539 4 56 7.295501428351461 5 1 2.4031473844628053 5 2 3.7312154461130405 5 3 3.1735833180797357 5 4 5.682727063807441 5 5 3.2960223825952752 5 6 2.8603614635407553 5 7 2.819421082560943 5 8 2.835509036429819 5 9 2.885868406612154 5 10 2.2994973500440707 5 11 4.282699768045234 5 12 6.271062930613962 5 13 7.164932862877038 5 14 6.2478044892861995 5 15 6.493715926441556 5 16 7.6286835172120355 5 17 7.094599327932773 5 18 7.343566036711064 5 19 5.72983759958003 5 20 4.929051176188892 5 21 3.1886289433209356 5 22 4.195388799936126 5 23 4.145351090360812 5 24 3.5074085536244795 5 25 2.7426376882469867 5 26 2.5304719707780805 5 27 2.162832516940192 5 28 2.588647517510772 5 29 3.058389122027357 5 30 2.8150962011797276 5 31 1.8704275033565168 5 32 3.8632362390347765 5 33 1.8865092526347027 5 34 1.0941081188645683 5 35 2.3745117744889583 5 36 3.9191218536265957 5 37 3.6568954684372272 5 38 4.206114287283114 5 39 5.948015416643042 5 40 6.797228605524094 5 41 2.0971223422826775 5 42 8.694749222504953 5 43 12.10261215105967 5 44 11.496688457577616 5 45 7.923551076136299 5 46 8.48370104607805 5 47 9.398172504246187 5 48 7.944626034324475 5 49 11.039992836337895 5 50 7.498270357132071 5 51 7.296053347483927 5 52 6.9449592219921925 5 53 7.604832344017996 5 54 5.723967074648947 5 55 5.830897749792204 5 56 6.698279206129239 6 1 2.1531473844628053 6 2 2.556215446113036 6 3 2.4235833180797357 6 4 4.432727063807441 6 5 2.5210223825952767 6 6 2.4603614635407567 6 7 2.719421082560949 6 8 2.7605090364298164 6 9 3.21086840661215 6 10 2.349497350044068 6 11 3.132699768045235 6 12 6.096062930613964 6 13 5.514932862877039 6 14 6.922804489286197 6 15 5.268715926441555 6 16 6.828683517212038 6 17 7.394599327932777 6 18 6.818566036711065 6 19 5.304837599580033 6 20 4.929051176188892 6 21 3.663628943320937 6 22 3.62038879993613 6 23 3.7953510903608105 6 24 3.957408553624475 6 25 3.9426376882469825 6 26 1.7054719707780777 6 27 2.7628325169401933 6 28 2.888647517510776 6 29 3.508389122027353 6 30 3.8150962011797276 6 31 4.345427503356518 6 32 4.913236239034781 6 33 3.8115092526347 6 34 3.744108118864567 6 35 3.024511774488957 6 36 3.64412185362659 6 37 3.0818954684372315 6 38 5.256114287283111 6 39 5.473015416643047 6 40 3.8472286055240907 6 41 -0.7528776577173204 6 42 8.469749222504952 6 43 12.05261215105967 6 44 10.396688457577614 6 45 6.3985510761363 6 46 9.158701046078047 6 47 9.798172504246192 6 48 10.244626034324472 6 49 11.389992836337896 6 50 9.798270357132072 6 51 8.571053347483929 6 52 9.54495922199219 6 53 8.154832344017997 6 54 10.398967074648944 6 55 9.830897749792204 6 56 9.648279206129239 7 1 2.1352902416056665 7 2 3.659786874684464 7 3 2.9200118895083094 7 4 5.829155635236006 7 5 2.967450954023846 7 6 3.02107574925504 7 7 3.4979925111323737 7 8 3.7533661792869637 7 9 3.6037255494692957 7 10 3.474497350044068 7 11 4.5219854823309475 7 12 5.835348644899678 7 13 7.06136143430561 7 14 7.447804489286195 7 15 7.42228735501298 7 16 8.017969231497748 7 17 7.369599327932772 7 18 6.157851750996773 7 19 6.011980456722888 7 20 5.521908319046034 7 21 4.3707718004637925 7 22 3.8953887999361285 7 23 4.148922518932238 7 24 4.185979982195903 7 25 3.7676376882469853 7 26 3.544757685063793 7 27 2.5128325169401933 7 28 2.4600760889393456 7 29 2.9476748363130696 7 30 2.8650962011797247 7 31 2.881141789070803 7 32 4.570379096177639 7 33 2.7579378240632693 7 34 2.6548224045788515 7 35 3.2030832030603875 7 36 1.8976932821980235 7 37 1.4640383255800842 7 38 2.5739714301402543 7 39 5.005158273785899 7 40 -1.6706285373330516 7 41 -11.845734800574464 7 42 4.516177793933522 7 43 9.931183579631096 7 44 7.043117029006186 7 45 4.569979647564871 7 46 6.590843903220907 7 47 7.491029647103332 7 48 7.662483177181617 7 49 10.589992836337895 7 50 7.376841785703501 7 51 9.299624776055357 7 52 9.144959221992192 7 53 8.808403772589426 7 54 10.681109931791802 7 55 10.238040606935062 7 56 9.216136348986382 8 1 2.2495759558913804 8 2 3.0883583032558946 8 3 2.9200118895083094 8 4 5.62915563523601 8 5 2.910308096880989 8 6 2.992504320683608 8 7 3.155135368275232 8 8 3.467651893572672 8 9 3.6037255494692957 8 10 2.360211635758354 8 11 3.6362711966166685 8 12 5.549634359185394 8 13 6.4327900057341765 8 14 7.019233060714772 8 15 7.165144497870127 8 16 6.960826374354891 8 17 6.741027899361345 8 18 7.100708893853916 8 19 5.640551885294315 8 20 5.207622604760317 8 21 3.2279146576066537 8 22 3.9811030856504104 8 23 3.520351090360812 8 24 3.6716942679101905 8 25 4.139066259675552 8 26 2.34475768506379 8 27 2.4842610883687612 8 28 3.060076088939347 8 29 2.8905319791702127 8 30 3.0650962011797276 8 31 2.0525703604993737 8 32 4.570379096177639 8 33 2.7007949669204123 8 34 2.254822404578853 8 35 3.1745117744889555 8 36 -0.6165924320876925 8 37 -1.707390245848483 8 38 0.3168285729973981 8 39 1.4908725595001862 8 40 -2.2134856801901925 8 41 -11.760020514860178 8 42 4.601892079647808 8 43 9.759755008202525 8 44 8.214545600434757 8 45 4.941408218993441 8 46 6.847986760363764 8 47 8.319601075674761 8 48 7.662483177181617 8 49 9.67570712205218 8 50 6.748270357132071 8 51 7.813910490341069 8 52 7.659244936277908 8 53 7.8084037725894255 8 54 6.7668242175060875 8 55 8.352326321220776 8 56 7.616136348986384 9 1 2.2781473844628053 9 2 3.80264401754161 9 3 2.8628690323654524 9 4 5.057727063807441 9 5 2.4817366683095585 9 6 2.5639328921121844 9 7 2.7551353682752335 9 8 2.9533661792869594 9 9 2.375154120897868 9 10 2.1030687786154942 9 11 3.5505569109023796 9 12 4.692491502042532 9 13 5.54707572001989 9 14 5.304947346429056 9 15 6.222287355012984 9 16 6.275112088640608 9 17 6.5981707565042065 9 18 5.8149946081396315 9 19 5.269123313865748 9 20 4.8933368904746075 9 21 2.913628943320937 9 22 3.409674514221841 9 23 2.434636804646523 9 24 3.957408553624475 9 25 3.024780545389838 9 26 3.0304719707780805 9 27 2.3699753740830474 9 28 2.4886475175107776 9 29 3.461960550598782 9 30 2.8365247726082927 9 31 2.766856074785089 9 32 4.341807667606211 9 33 3.7865092526347013 9 34 2.454822404578856 9 35 3.7173689173461 9 36 1.9262647107694484 9 37 1.5497526112943731 9 38 3.1739714301402557 9 39 3.6051582737859 9 40 -0.7277713944759086 9 41 -4.13144908628875 9 42 6.201892079647809 9 43 9.245469293916813 9 44 8.814545600434759 9 45 6.112836790422012 9 46 8.90512961750662 9 47 10.376743932817622 9 48 10.205340320038761 9 49 13.475707122052185 9 50 8.691127499989214 9 51 9.385339061769638 9 52 9.944959221992189 9 53 8.236975201160856 9 54 10.709681360363234 9 55 8.409469178363633 9 56 7.701850634700669 10 1 2.360500325639279 10 2 3.5018036814071536 10 3 3.001524494550324 10 4 5.828315299101554 10 5 2.401904735536448 10 6 2.841243816481928 10 7 2.092950494325649 10 8 2.492861977606289 10 9 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1.3889057249892502 115 31 1.1382846462136627 115 32 -5.210573284774746 115 33 -0.21349074736529872 115 34 1.159584309340758 115 35 -0.7302501302729496 115 36 3.8976932821980235 115 37 2.79737165891342 115 38 5.593019049187873 115 39 6.86230113092876 115 40 10.215085748381238 115 41 8.220931866092204 115 42 2.6304635082192362 115 43 1.5978502462977637 115 44 -0.4902163043271486 115 45 2.798551076136299 115 46 -1.8377275253505232 115 47 -5.9851608290871425 115 48 -4.4422787275802875 115 49 -4.752864306519246 115 50 1.0244608333225464 115 51 1.5377200141505938 115 52 1.5735306505636188 115 53 -0.08683432264867008 115 54 0.7668242175060875 115 55 1.4189929878874423 115 56 3.4066125394625715 116 1 -0.21750478945023843 116 2 0.07469370698260036 116 3 0.45293114416669056 116 4 1.3359879333726568 116 5 0.022109339117015736 116 6 0.04840494180162125 116 7 0.7476819521261646 116 8 0.7409438190385131 116 9 0.5304336240034573 116 10 0.12667126308754462 116 11 -2.131430666737373 116 12 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-1.1558918811354317 119 35 -0.012988225511044504 119 36 2.531621853626593 119 37 1.73189546843723 119 38 4.956114287283114 119 39 6.8980154166430445 119 40 5.697228605524092 119 41 6.134622342282679 119 42 -0.09275077749504845 119 43 -2.809887848940331 119 44 -6.040811542422386 119 45 -3.763948923863701 119 46 -8.70379895392195 119 47 -13.15182749575381 119 48 -9.930373965675525 119 49 -8.797507163662104 119 50 -7.389229642867928 119 51 -8.003946652516074 119 52 -8.355040778007808 119 53 -13.670167655982002 119 54 -11.938532925351055 119 55 -13.069102250207795 119 56 -9.23922079387076 120 1 0.47814738446280813 120 2 1.7680575513761951 120 3 1.1485833180797371 120 4 3.857727063807438 120 5 1.1960223825952738 120 6 0.6616772530144388 120 7 0.6904737141398911 120 8 0.9789300890614001 120 9 0.50297366977005 120 10 0.7902868237282803 120 11 -9.4975633898495 120 12 -3.563147595701828 120 13 -7.2048039792282275 120 14 -9.825879721240117 120 15 -8.627336705137395 120 16 -13.65421121963007 120 17 -14.535663829961962 120 18 -4.651170805394202 120 19 -5.097793979367339 120 20 -7.420948823811109 120 21 -2.6653184251001143 120 22 -1.599348042169133 120 23 -1.763859435954977 120 24 -9.284696709533417 120 25 -1.884993890700386 120 26 -0.9379490818535032 120 27 -0.8029569567440191 120 28 -0.2902998509102801 120 29 -0.5350319306042266 120 30 -0.8717459040834328 120 31 -5.530888286117168 120 32 -10.73939533991259 120 33 -4.108227589470562 120 34 -12.715102407451223 120 35 -3.7728566465636746 120 36 -1.057193935847092 120 37 -2.3404729526154 120 38 0.5679563925462716 120 39 2.6216996271693596 120 40 -0.611981920791699 120 41 3.855017079124785 120 42 -1.1289349880213635 120 43 -6.076335217361384 120 44 -7.201995752948701 120 45 -11.096185765968965 120 46 -12.95050948023774 120 47 -11.809722232595915 120 48 -10.048795018307104 120 49 -14.820533479451576 120 50 -8.39909806392056 120 51 -8.602630863042389 120 52 -7.539251304323599 120 53 -8.47279923492937 120 54 -11.060243451666844 120 55 -8.766470671260427 120 56 -4.558299741239182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 277.0 5 6.0 6 11.0 7 26.0 8 44.0 9 56.0 10 37.0 11 30.0 12 29.0 13 38.0 14 55.0 15 94.0 16 115.0 17 159.0 18 178.0 19 264.0 20 322.0 21 360.0 22 488.0 23 551.0 24 655.0 25 712.0 26 812.0 27 792.0 28 864.0 29 885.0 30 967.0 31 1212.0 32 1436.0 33 1646.0 34 2022.0 35 2445.0 36 2838.0 37 2771.0 38 1611.0 39 476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.05869324473976 0.7989242208511311 0.12260718240784686 0.019775352001265623 8 99.30390760955545 0.5220692928334125 0.07910140800506249 0.09492168960607499 9 97.37726967047747 1.4794889038332213 0.4984374381898018 0.6448039874995055 10 30.323524758740707 43.70352792279702 8.072298686916628 17.90064863154564 11 25.118652112007595 30.102040816326532 24.624268311975953 20.155038759689923 12 32.3406106628698 14.598164847334283 22.832621420661287 30.22860306913463 13 27.349311817750355 15.266571744977062 24.086378737541526 33.29773769973106 14 20.356012658227847 19.707278481012658 24.553006329113924 35.383702531645575 15 23.705991841907252 21.777355352263278 24.470318007207638 30.04633479862184 16 25.54302670623145 21.83580613254204 25.475766567754697 27.14540059347181 17 25.794074601479373 25.240299038803844 23.0647521854357 25.90087417428108 18 28.125618004192543 28.394573428786142 21.148597872087965 22.331210694933354 19 25.66364679352771 28.8918779918503 20.06567235035803 25.378802864263957 20 25.164200364010448 27.249347155179237 21.872279813246813 25.714172667563506 21 25.570631749673645 24.197950868309665 24.965386289014596 25.266031093002095 22 25.640721404841006 23.876760006328112 24.51748141116912 25.965037177661763 23 23.485208036703053 27.373042240151875 26.740230976111373 22.4015187470337 24 22.096978326214206 29.83705109950957 22.041607340610664 26.02436323366556 25 22.67046353425091 32.06771080525233 22.496440436639773 22.765385223856985 26 21.111859571264414 30.922851369021675 25.75979712327139 22.205491936442527 27 22.94362017804154 22.77349159248269 29.554896142433236 24.727992087042534 28 20.40727387979874 26.619389089180302 29.17475535834555 23.798581672675407 29 19.316428656196845 25.99390798686657 31.58352782942363 23.106135527512954 30 19.116774168018676 25.804281587590516 35.48336037355071 19.595583870840095 31 18.15876035350533 26.390044782625928 36.622676653588556 18.828518210280187 32 21.30058146434081 21.071160159803807 36.268343815513624 21.359914560341757 33 20.068957317798123 23.25129790353902 38.28320057068125 18.39654420798161 34 19.79410017818254 23.71411601663037 39.659473371609586 16.83231043357751 35 19.020104479974673 25.50656957416495 39.04939053348108 16.423935412379294 36 18.87726804532129 23.68275097060455 37.85357737104825 19.58640361302591 37 18.32130679565459 25.49758147648878 37.84394576163667 18.337165966219967 38 21.558997108567354 25.69414187824296 37.27571592664475 15.471145086544935 39 17.456032395093096 22.089007106276547 34.65401564174838 25.800944856881973 40 11.417776894014715 18.03539471067807 26.931795585603503 43.61503280970372 41 14.830322606372373 18.909757945527776 28.930892850021934 37.32902659807792 42 15.48402441924998 28.49044636486165 39.06287164037104 16.962657575517323 43 10.39723798563435 15.968887654271995 29.080519068216994 44.55335529187666 44 10.90186730234406 17.624155740961463 26.769964243146603 44.704012713547876 45 16.5696408063047 26.086095600174254 38.16878539463784 19.175478198883212 46 16.67524243023946 27.120522461903818 39.14902038393034 17.05521472392638 47 16.98449121696471 22.752808988764045 36.14495964551353 24.117740148757715 48 15.965488581944829 26.417857284204693 39.84248228915186 17.774171844698618 49 12.230329949238579 17.457170050761423 28.03378807106599 42.27871192893401 50 14.814814814814813 25.620588468554207 41.946228884130385 17.618367832500596 51 19.34050236101742 26.356096980278558 38.02230070235308 16.28109995635094 52 16.288856246774387 28.397316288856246 37.13922744054944 18.17460002381992 53 17.172637920101458 29.81531388712746 37.19086873811034 15.821179454660747 54 15.711967063853372 24.41708562606389 34.41668975891691 25.454257551165828 55 17.148743957524367 25.937079007845316 40.763927411046836 16.150249623583484 56 15.502616140795942 26.37149199302363 38.33835420960837 19.787537656572063 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.0 10 1.5 11 1.0 12 9.0 13 34.0 14 51.0 15 72.5 16 94.0 17 97.0 18 100.0 19 136.5 20 173.0 21 189.0 22 248.0 23 291.0 24 348.5 25 406.0 26 514.0 27 622.0 28 761.5 29 973.5 30 1046.0 31 1113.5 32 1181.0 33 1158.0 34 1135.0 35 1039.5 36 944.0 37 870.0 38 751.5 39 707.0 40 709.5 41 712.0 42 691.5 43 671.0 44 731.5 45 792.0 46 779.0 47 840.5 48 915.0 49 917.5 50 920.0 51 1008.5 52 1097.0 53 1082.5 54 1112.0 55 1156.0 56 1157.5 57 1159.0 58 1147.0 59 1135.0 60 1191.0 61 1247.0 62 1170.5 63 1069.5 64 1045.0 65 1014.5 66 984.0 67 830.5 68 677.0 69 590.5 70 504.0 71 400.5 72 238.0 73 179.0 74 142.0 75 105.0 76 77.5 77 50.0 78 36.5 79 16.5 80 10.0 81 7.5 82 5.0 83 3.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.019775352001265623 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0158202816010125 15 0.13051732320835313 16 0.03559563360227812 17 0.011865211200759373 18 0.003955070400253125 19 0.02768549280177187 20 0.039550704002531245 21 0.019775352001265623 22 0.0 23 0.0 24 0.0 25 0.0 26 0.18588830881189686 27 0.03559563360227812 28 0.17006802721088435 29 0.019775352001265623 30 0.05141591520329061 31 0.20170859041290937 32 0.011865211200759373 33 0.20170859041290937 34 0.11469704160734062 35 0.06328112640405 36 0.16611295681063123 37 0.24521436481569373 38 0.14633760480936558 39 0.37573168802404683 40 0.5497547856351843 41 0.8186995728523968 42 0.22939408321468124 43 0.3361809840215156 44 0.4508780256288562 45 0.13051732320835313 46 0.07514633760480936 47 0.031640563202025 48 0.06723619680430312 49 0.2689447872172125 50 0.26103464641670626 51 0.32827084322100936 52 0.37573168802404683 53 0.20566366081316245 54 0.09096661920582187 55 0.18193323841164374 56 0.22148394241417496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 25284.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.82423667141275 #Duplication Level Percentage of deduplicated Percentage of total 1 94.87023166695697 86.16516373991458 2 3.562097195610521 6.470495174814111 3 0.7402891482320153 2.0170859041290936 4 0.3396620797770423 1.2339819648789747 5 0.13499390350113222 0.6130359120392342 6 0.10451140916216688 0.5695301376364499 7 0.07402891482320154 0.4706533776301218 8 0.021773210242118098 0.15820281601012498 9 0.02612785229054172 0.21357380161366873 >10 0.12192997735586135 1.775826609713653 >50 0.00435464204842362 0.31245056161999685 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GACACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 79 0.31245056161999685 No Hit GACACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC 31 0.12260718240784686 No Hit GACACTGGGGCCGCATAGCCTCTCCACCCAAGCGGCCGGAGACCCTGCCTGCAATC 27 0.10678690080683437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.003955070400253125 0.0 0.0 0.0 0.0 11 0.0158202816010125 0.0 0.0 0.0 0.0 12 0.019775352001265623 0.0 0.0 0.0 0.0 13 0.019775352001265623 0.0 0.0 0.0 0.0 14 0.023730422401518746 0.0 0.0 0.0 0.0 15 0.02768549280177187 0.0 0.0 0.0 0.0 16 0.02768549280177187 0.0 0.0 0.0 0.0 17 0.02768549280177187 0.0 0.0 0.0 0.0 18 0.02768549280177187 0.0 0.0 0.0 0.0 19 0.031640563202025 0.0 0.0 0.0 0.0 20 0.031640563202025 0.0 0.0 0.0 0.0 21 0.03559563360227812 0.0 0.0 0.0 0.0 22 0.04350577440278437 0.0 0.0 0.0 0.0 23 0.04746084480303749 0.0 0.0 0.0 0.0 24 0.05537098560354374 0.0 0.0 0.0 0.0 25 0.06328112640405 0.0 0.0 0.0 0.0 26 0.07119126720455624 0.0 0.0 0.0 0.0 27 0.07910140800506249 0.0 0.0 0.0 0.0 28 0.08305647840531562 0.0 0.0 0.0 0.0 29 0.09096661920582186 0.0 0.0 0.0 0.0 30 0.09096661920582186 0.0 0.0 0.0 0.0 31 0.09096661920582186 0.0 0.0 0.0 0.0 32 0.09096661920582186 0.0 0.0 0.0 0.0 33 0.09096661920582186 0.0 0.0 0.0 0.0 34 0.09096661920582186 0.0 0.0 0.0 0.0 35 0.09096661920582186 0.0 0.0 0.0 0.0 36 0.09096661920582186 0.0 0.0 0.0 0.0 37 0.09096661920582186 0.0 0.0 0.0 0.0 38 0.09096661920582186 0.0 0.0 0.0 0.0 39 0.09096661920582186 0.0 0.0 0.0 0.0 40 0.09096661920582186 0.0 0.0 0.0 0.0 41 0.09096661920582186 0.0 0.0 0.0 0.0 42 0.09096661920582186 0.0 0.0 0.0 0.0 43 0.09096661920582186 0.0 0.0 0.0 0.0 44 0.09492168960607499 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAACCA 15 0.007960013 50.585514 39 CGACCCT 15 0.007960013 50.585514 40 GAGACCC 20 3.8755895E-4 50.58551 39 GATACCC 15 0.008087769 50.382767 35 AGAGCGC 15 0.008087769 50.382767 35 ATACCCA 15 0.008087769 50.382767 36 ATAGGAC 15 0.008152217 50.282 34 TGATACC 15 0.008152217 50.282 34 TAGAGCG 15 0.008152217 50.282 34 ATTCTGA 15 0.008152217 50.282 34 CGCTGCA 15 0.008217045 50.18164 44 CCCAGCC 15 0.008217045 50.18164 43 CCAGCCC 15 0.008217045 50.18164 44 GTCTTCA 15 0.008217045 50.18164 50 AAATGGT 15 0.008217045 50.18164 50 CTTCTCT 30 9.821597E-7 50.18164 44 TGAGTCG 20 4.0325962E-4 50.181633 50 CAGCCCC 15 0.008282256 50.081676 45 CAGTCTT 15 0.008282256 50.081676 48 GAAGAGC 15 0.008282256 50.081676 33 >>END_MODULE