FastQCFastQC Report
Fri 17 Jun 2016
SRR1526343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142051
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG8120.571625683733307No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6770.47658939395006017No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG5550.39070474688668155No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC5150.36254584621016395No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG4100.28862873193430527No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4030.2837009243159147No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC3680.2590618862239618No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG3370.23723873819966068No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2900.20415202990475254No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2780.19570435970179723No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG2580.18162490936353845No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC1970.13868258583184914No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC1930.13586669576419735No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC1700.11967532787519974No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG1580.11122765767224446No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTC1480.10418793250311507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT203.8871242E-450.71972740
AGACTAC150.00803273650.59247237
ACCCTAC150.00803273650.59247241
TCTGAAA150.00812421250.44781536
TGATACC204.062617E-450.2681634
ATATGCA252.0133199E-550.2681634
TAAAGTG150.00823964450.26815834
TAGGATC150.00823964450.26815834
AGATAGA150.008262875550.23238449
CGATTCA750.050.23238449
CGATTAA1350.050.23238449
ACGATTA1350.050.1966648
ACGATTC800.050.19665548
GTTCGCG150.00828615750.19665548
TCATATG150.00828615750.19665548
CTCATAT150.00829781550.1788147
AACGATT1600.050.1788147
CCCTACT150.00830948650.1609842
TCCCACA150.00830948650.1609842
TTGTGCA150.00839152450.0365150