##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526343_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 142051 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.67226559475118 39.0 38.0 40.0 33.0 40.0 2 36.99522706633533 39.0 38.0 39.0 32.0 40.0 3 37.07597271402525 39.0 38.0 40.0 32.0 40.0 4 35.45485072262779 39.0 35.0 39.0 23.0 40.0 5 37.03923238836756 39.0 38.0 39.0 32.0 40.0 6 37.13624684092333 39.0 38.0 39.0 33.0 40.0 7 37.16529274697116 39.0 38.0 39.0 33.0 40.0 8 37.019049496307666 39.0 37.0 39.0 33.0 40.0 9 37.03835242272142 39.0 38.0 39.0 33.0 40.0 10 36.70221258562066 39.0 36.0 39.0 31.0 40.0 11 34.92382313394485 38.0 33.0 39.0 28.0 40.0 12 33.10437096535751 37.0 31.0 39.0 19.0 40.0 13 32.91840958528979 37.0 31.0 39.0 18.0 40.0 14 32.20907983752314 36.0 29.0 39.0 15.0 40.0 15 32.90873700290741 37.0 30.0 39.0 18.0 40.0 16 32.80509816896748 36.0 30.0 39.0 19.0 40.0 17 32.82766048813454 36.0 30.0 39.0 19.0 40.0 18 33.11355076697806 36.0 31.0 39.0 21.0 40.0 19 33.80318336372148 36.0 31.0 38.0 25.0 40.0 20 33.93355907385376 37.0 31.0 39.0 25.0 40.0 21 35.27069151220336 38.0 35.0 39.0 27.0 40.0 22 35.023336688935665 38.0 35.0 39.0 27.0 40.0 23 35.25152938029299 38.0 35.0 39.0 27.0 40.0 24 34.809364242419974 38.0 33.0 39.0 26.0 40.0 25 35.10209009440271 38.0 34.0 39.0 27.0 40.0 26 35.295224954417776 38.0 35.0 39.0 28.0 40.0 27 35.2430676306397 38.0 35.0 39.0 28.0 40.0 28 35.02309733827991 38.0 35.0 39.0 27.0 40.0 29 34.906012629266954 38.0 35.0 39.0 27.0 40.0 30 34.65261068207897 38.0 34.0 39.0 26.0 40.0 31 34.193620601051734 38.0 33.0 39.0 24.0 40.0 32 33.25680917416984 38.0 33.0 39.0 18.0 40.0 33 34.53661713046723 38.0 34.0 39.0 26.0 40.0 34 34.146461482143735 38.0 33.0 39.0 24.0 40.0 35 34.26326460214993 38.0 33.0 39.0 26.0 40.0 36 32.26218048447389 35.0 31.0 38.0 21.0 39.0 37 32.05713440947265 35.0 31.0 38.0 19.0 39.0 38 31.29269769308206 35.0 30.0 38.0 12.0 39.0 39 29.28784732244053 34.0 26.0 38.0 5.0 39.0 40 25.164504297752217 30.0 11.0 37.0 4.0 39.0 41 22.190150016543353 26.0 9.0 33.0 4.0 37.0 42 25.725443678678783 30.0 20.0 34.0 4.0 37.0 43 21.670752053839816 26.0 8.0 33.0 4.0 37.0 44 22.88856819029785 28.0 10.0 33.0 4.0 37.0 45 25.279012467353276 30.0 19.0 34.0 4.0 37.0 46 25.012882697059506 30.0 15.0 36.0 4.0 38.0 47 24.375829807604312 30.0 10.0 35.0 4.0 38.0 48 24.3390261244201 29.0 10.0 35.0 4.0 38.0 49 21.946132023005823 26.0 5.0 35.0 4.0 38.0 50 24.197091185560115 29.0 4.0 35.0 4.0 38.0 51 25.631174718938972 32.0 4.0 38.0 4.0 39.0 52 25.52122125152234 32.0 4.0 38.0 4.0 39.0 53 25.14977015297323 32.0 4.0 37.0 4.0 39.0 54 24.2591674821015 31.0 4.0 37.0 4.0 39.0 55 24.398209093916975 31.0 4.0 37.0 4.0 39.0 56 23.62673969208242 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.6226046227746593 1 2 1.3478836035240604 1 3 0.974290655081262 1 4 2.5743988639990505 1 5 1.0365198066553205 1 6 0.9947431178775261 1 7 1.1271062203711395 1 8 1.1307393109721247 1 9 1.2223555417743768 1 10 1.270835288297711 1 11 -5.245953337644146 1 12 -8.426923922429221 1 13 -21.876973872705122 1 14 -18.77087840632327 1 15 -12.251503960321863 1 16 -21.975390529339023 1 17 -21.631534753015956 1 18 -21.62247001499543 1 19 -7.384241434821178 1 20 -11.319473944305933 1 21 -8.51083788751448 1 22 -3.212634100961477 1 23 -3.7813638597232675 1 24 -3.6175626391265467 1 25 -3.4685247293240025 1 26 2.282581275941183 1 27 1.6283866858935383 1 28 1.654067742663372 1 29 1.066536594105031 1 30 1.4598033758067217 1 31 0.2330581617362384 1 32 -13.812847813971167 1 33 0.5726948166070827 1 34 0.1350810040060182 1 35 0.2290788878643255 1 36 2.3549280352700563 1 37 2.2209994224609453 1 38 3.4837895908814573 1 39 5.212661920603939 1 40 7.402311685701889 1 41 -2.721154419059541 1 42 -8.049118947184471 1 43 -12.821566242744124 1 44 -15.746537349293352 1 45 -12.594035006337183 1 46 -17.636467465917324 1 47 -17.654704870882988 1 48 -15.793999402705149 1 49 -13.768314256738293 1 50 -16.31543507984705 1 51 -17.367050921872092 1 52 -17.53299285203277 1 53 -17.23160514280613 1 54 -17.912994446231586 1 55 -16.888959276419737 1 56 -16.15271543031974 2 1 0.7274909660715991 2 2 1.698053641111315 2 3 1.5059892410844853 2 4 2.6547997947311046 2 5 1.4744292392667404 2 6 1.5284463575410356 2 7 1.597698667122529 2 8 1.7717631162343395 2 9 1.67039563484758 2 10 1.7030887337961929 2 11 -3.8332094679466344 2 12 -10.437090380277798 2 13 -12.70815339659988 2 14 -15.074690828016488 2 15 -9.067755973924868 2 16 -3.9502965611986944 2 17 -2.703666487399346 2 18 -9.978439229242788 2 19 -2.4282006258002333 2 20 -3.6292645295932076 2 21 2.0751295368635425 2 22 2.3241477139660347 2 23 1.9636871515529108 2 24 2.6057772570610354 2 25 2.2328534700674467 2 26 2.0170720581140884 2 27 2.3879714362067688 2 28 2.2344865720888194 2 29 2.3058421248012877 2 30 2.591788340904273 2 31 -0.9278152945014568 2 32 -9.394805931028621 2 33 -0.7527392267077531 2 34 -0.4426530214101234 2 35 -0.5834501975124411 2 36 3.6753862418209806 2 37 3.7370545504366 2 38 5.063599864731465 2 39 7.0545806605359225 2 40 9.963686305354653 2 41 6.259407599913072 2 42 2.369817870427841 2 43 -2.6117935561502463 2 44 -8.697494929390006 2 45 0.19397286908791145 2 46 -11.647851034916787 2 47 -12.220053000469528 2 48 -9.119039674765288 2 49 -11.549771210380676 2 50 -8.625798423322976 2 51 -8.436247270538638 2 52 -8.294045295204418 2 53 -9.090348654529773 2 54 -14.386665468246978 2 55 -7.9425121670587195 2 56 -7.616685360514836 3 1 0.9092983240904147 3 2 1.6986727624352866 3 3 1.940302849847427 3 4 2.9553831975171505 3 5 1.420946681819423 3 6 1.469201209309098 3 7 1.7975557929708472 3 8 2.010863009078726 3 9 2.0538460456107686 3 10 1.7921469550130027 3 11 0.005818987821911037 3 12 1.1166183945763528 3 13 -4.6054506938971755 3 14 -8.414516997798604 3 15 -3.556052199666027 3 16 2.0503701848424996 3 17 1.8843797085763683 3 18 1.9234062285498048 3 19 0.7670845271941715 3 20 0.5209438285446666 3 21 2.784666386488496 3 22 3.109360239266664 3 23 2.697441169888428 3 24 2.7188145234022656 3 25 2.8402829259550515 3 26 2.4615059192880366 3 27 2.401353981747903 3 28 1.9604539491575181 3 29 2.5442038345286377 3 30 2.0516287981015537 3 31 2.4390855270249148 3 32 1.50765864808794 3 33 2.0832412471581776 3 34 2.846024202068861 3 35 1.8045959984632702 3 36 4.089268846893013 3 37 4.482690935720566 3 38 5.520344715308916 3 39 8.032482791742023 3 40 10.539254825416567 3 41 9.072147211771629 3 42 6.470996582179243 3 43 0.9324378999754828 3 44 2.8457840323911583 3 45 6.924155033631308 3 46 0.5392188543557452 3 47 -4.622934295861839 3 48 -0.10082322042548597 3 49 -9.66871394165821 3 50 -0.7839124845207373 3 51 -1.6579165168774885 3 52 -1.2241560226719734 3 53 -1.8631073162752187 3 54 -4.338469254411997 3 55 -1.7486319432225486 3 56 1.3905059717866273 4 1 1.2274324660130986 4 2 1.7793219223795944 4 3 1.9074634425586865 4 4 2.640853380784691 4 5 1.566321131158631 4 6 1.8316170607117357 4 7 2.0220346914585576 4 8 2.0532185976898205 4 9 2.0004295648815145 4 10 1.630314661022119 4 11 3.8811932464560783 4 12 4.215746872559453 4 13 4.8664107779642976 4 14 4.402482449156793 4 15 6.055023948855052 4 16 5.668025657123522 4 17 5.424799641066784 4 18 5.136080485276924 4 19 3.7939357963361857 4 20 4.083008782680103 4 21 2.539877401611406 4 22 3.2541816439999636 4 23 2.7952537831195414 4 24 2.9331669844507573 4 25 2.587293624507595 4 26 2.5143693554113824 4 27 2.1745866228219555 4 28 2.2555699931722444 4 29 2.3387191576783195 4 30 2.486207435323365 4 31 2.440477673791513 4 32 3.3724106361879436 4 33 2.5296054556369256 4 34 2.699829721072618 4 35 2.5268341127718656 4 36 4.256479022913766 4 37 4.479162892544945 4 38 5.296266297397899 4 39 7.623458629413893 4 40 9.7086962503646 4 41 7.667223207728682 4 42 7.498915799525772 4 43 8.223587279230589 4 44 10.933603701708627 4 45 8.525715000830044 4 46 7.90867172160597 4 47 7.838400257983729 4 48 7.1504115946859805 4 49 5.220791560182093 4 50 7.216602419077866 4 51 5.657891023599657 4 52 6.059880058720935 4 53 5.447360483179363 4 54 1.246117964536456 4 55 5.988481163934612 4 56 4.634303290473813 5 1 1.6101164486970845 5 2 2.6442569873146624 5 3 2.2819223170175604 5 4 3.546048185979501 5 5 2.1567973216348193 5 6 2.0701451992398745 5 7 2.1969264663503267 5 8 2.125945870417091 5 9 2.3571395215914706 5 10 2.2891891198965766 5 11 4.025349090611925 5 12 6.041287998100582 5 13 6.218358829912347 5 14 6.098153444827787 5 15 6.393119186950294 5 16 6.581878470976335 5 17 6.165059381326522 5 18 5.605344554540995 5 19 4.71774532014571 5 20 4.854004453675778 5 21 3.1004834622174613 5 22 3.3585106483289664 5 23 2.743738631604387 5 24 3.6167167680005434 5 25 3.1730079102218838 5 26 2.80960745064948 5 27 2.850777099012433 5 28 2.9105483481505985 5 29 2.746511365470525 5 30 2.896164145280075 5 31 3.3694820027958414 5 32 4.0161335799108855 5 33 3.013155239186709 5 34 3.103292924535822 5 35 2.770989956927714 5 36 3.931370797805535 5 37 3.8289464423284905 5 38 4.351244652376252 5 39 6.606575512530778 5 40 6.803068544736892 5 41 3.3235002640057374 5 42 8.426188526798498 5 43 11.958219313862624 5 44 11.03879850690343 5 45 8.402338377453418 5 46 9.772740985675231 5 47 10.4072314268149 5 48 9.779415923690308 5 49 10.588324027714563 5 50 10.305347007822455 5 51 9.418064183772813 5 52 9.7217848206257 5 53 9.356884292703175 5 54 8.854775973194464 5 55 9.286316661770108 5 56 8.060277316447841 6 1 1.5614493263457518 6 2 2.3382191654873665 6 3 1.8978940645682556 6 4 3.3068365204520447 6 5 2.2409622794839876 6 6 1.8676161493424033 6 7 2.2671930418800628 6 8 2.237998729838374 6 9 2.479809833735466 6 10 2.429585565556181 6 11 3.762031706241906 6 12 6.341287998100579 6 13 6.078759832418612 6 14 6.610502499282106 6 15 6.330485329579595 6 16 6.466385192325163 6 17 6.440520762051065 6 18 5.918263214828077 6 19 4.710089817753364 6 20 4.675171006421273 6 21 2.9064757155781393 6 22 3.0062891855811884 6 23 2.5721961390092645 6 24 3.179008861871587 6 25 2.6201029204747854 6 26 1.6914028482343468 6 27 2.402428955927448 6 28 2.193596878567547 6 29 2.2576072870927675 6 30 1.9093106479002628 6 31 2.5589556870063674 6 32 3.6683778268919767 6 33 2.6861331384804004 6 34 2.7884603886506483 6 35 2.10236384619634 6 36 2.7217330671151743 6 37 2.617463188739979 6 38 3.7018256503256737 6 39 5.033346986670676 6 40 3.1773679295625925 6 41 1.466494112262744 6 42 6.073762005950925 6 43 11.37669732707748 6 44 9.301341227340888 6 45 7.090080459932345 6 46 9.41698796676432 6 47 9.611241451877554 6 48 9.625690701544034 6 49 11.239702473829851 6 50 9.53170836576276 6 51 9.053653156203893 6 52 9.114334376333147 6 53 9.190810016102585 6 54 9.581729721200844 6 55 8.867496885055598 6 56 7.718604953722846 7 1 1.3711676616350914 7 2 2.082576843559046 7 3 1.9447704751487365 7 4 3.3856259038591006 7 5 1.6748116971963682 7 6 1.8798487032830025 7 7 2.0253631240322676 7 8 2.074822779671358 7 9 1.7614971136849107 7 10 2.215199901567736 7 11 3.6341541220584617 7 12 5.633740828289259 7 13 5.63511534383867 7 14 6.79550295066786 7 15 5.629821792520822 7 16 6.10204918076969 7 17 5.645517602350786 7 18 5.186476630012695 7 19 4.199820791843827 7 20 4.362270401564366 7 21 2.869800623044057 7 22 3.383173721105244 7 23 3.11970448964572 7 24 3.1023861300850015 7 25 2.9424149183081383 7 26 2.056822185600062 7 27 1.9800673056611302 7 28 1.824160207988875 7 29 2.295388274724793 7 30 2.2617526448308425 7 31 2.1657084178901798 7 32 3.668784074070814 7 33 2.285302588692552 7 34 2.6297080188754407 7 35 2.3770546469546687 7 36 0.8918829271855913 7 37 0.8921539894983042 7 38 1.7118915526457918 7 39 3.458507228613435 7 40 -3.7792764687401963 7 41 -9.826364965104775 7 42 3.366350251865885 7 43 8.288767382146542 7 44 6.969256727927689 7 45 4.135941252565729 7 46 6.695876655037317 7 47 6.8587588302290925 7 48 6.922138295657966 7 49 8.65305897829857 7 50 6.241106217166568 7 51 8.188459511715315 7 52 8.485531451353463 7 53 8.670181687132644 7 54 9.246914337974339 7 55 8.564933013971366 7 56 7.328291692009387 8 1 1.538826573523714 8 2 2.0624724288310716 8 3 2.1651863022232902 8 4 3.0024513297612856 8 5 1.9692337525178516 8 6 1.9035709967433405 8 7 2.205386937917595 8 8 2.1243739795248118 8 9 2.2530248660205032 8 10 1.8298271226705012 8 11 3.8839251423918597 8 12 5.63255013402291 8 13 5.748918238141663 8 14 6.551181646399677 8 15 6.536993435678909 8 16 6.584051378974486 8 17 5.928582266208629 8 18 5.60340280696818 8 19 4.351725902669983 8 20 4.482622114332273 8 21 3.2063549370209756 8 22 3.3951264597020554 8 23 3.0038865742766063 8 24 3.151067207205351 8 25 2.929179893578336 8 26 2.4803887728871103 8 27 2.2108788095995777 8 28 2.9912695686776445 8 29 2.310958892053975 8 30 2.7067975248454914 8 31 2.9248218086210827 8 32 4.0854354754263795 8 33 2.8122305974853674 8 34 3.082904575021253 8 35 2.387633507551847 8 36 0.9204137936446486 8 37 1.1697231413576183 8 38 1.8128257896855438 8 39 3.0666772231179245 8 40 -2.5614252139316243 8 41 -9.494252856660005 8 42 4.617311966465628 8 43 9.355537852928737 8 44 7.502321391785539 8 45 5.5914276053776 8 46 8.236772423493079 8 47 9.065252693574028 8 48 8.9905116241064 8 49 11.256054032337769 8 50 8.947279508978255 8 51 10.055697101017387 8 52 9.722616998156909 8 53 9.993592568246402 8 54 11.028238756365983 8 55 10.717708247530627 8 56 9.736791417233785 9 1 1.5784993348464695 9 2 1.8092074344986102 9 3 1.726568195673586 9 4 2.9575299360399327 9 5 2.028250065168784 9 6 1.5408486075067387 9 7 1.7244101501791889 9 8 1.8033449518770865 9 9 1.8002625576079083 9 10 1.7280578653643062 9 11 3.586601205671144 9 12 5.7067702316031195 9 13 5.78541225026526 9 14 6.4131401501698235 9 15 5.7577070525533856 9 16 5.9128671100868 9 17 5.365204413958864 9 18 4.789100899718658 9 19 4.206070193242155 9 20 4.407859904485541 9 21 2.6328740834405764 9 22 2.729141540279656 9 23 2.3829216144421963 9 24 2.711882346922465 9 25 2.4611152343698137 9 26 2.03213585287331 9 27 1.7941176839773831 9 28 1.3625425468453045 9 29 1.5264485179965135 9 30 2.448037483447827 9 31 2.231410936805993 9 32 3.0625681943658094 9 33 1.6551905304605796 9 34 2.268775158038359 9 35 1.6762836480082015 9 36 0.8928646339186628 9 37 0.7751393357295093 9 38 1.9884455225720465 9 39 3.4925556914043554 9 40 -1.115858213783774 9 41 -3.658660722216162 9 42 5.474194328103792 9 43 9.795806937744665 9 44 8.598842016693133 9 45 6.166781210424169 9 46 9.104817369528266 9 47 9.444263092272958 9 48 9.100880753276968 9 49 11.709112038350288 9 50 9.75216837596362 9 51 9.011441026895852 9 52 9.572328692996983 9 53 8.868438559079774 9 54 8.363067005343364 9 55 8.921172837743036 9 56 7.398735136123932 10 1 1.464962105552793 10 2 1.6614381311289748 10 3 1.5036260738971947 10 4 2.9382712058407066 10 5 1.4105422366862683 10 6 1.6801236629871852 10 7 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41 9.660008200513673 114 42 6.092590643200616 114 43 0.5889071445504541 114 44 1.3525551206600461 114 45 4.784613509728551 114 46 -3.4952484323141846 114 47 -6.2401230705396 114 48 -4.2864570921827045 114 49 -13.438660099269566 114 50 -3.050473097997653 114 51 -1.9107717950631624 114 52 -1.469220470379593 114 53 -1.6002585644396845 114 54 -2.7081869897684996 114 55 -0.8747944493409996 114 56 2.229060385230909 115 1 0.4572206958013325 115 2 0.8043599474176233 115 3 1.1654487005439407 115 4 2.0547997947311103 115 5 1.0257805906180906 115 6 0.7500679791626581 115 7 0.5922932617171242 115 8 0.9050964495676723 115 9 0.8001253645773119 115 10 0.4958815265889811 115 11 -2.3737500084871748 115 12 -4.8839372271246475 115 13 -2.1099551984016784 115 14 -8.352168305493965 115 15 -3.372260478429375 115 16 -9.838584849486985 115 17 -9.28024306397592 115 18 -2.3261869769905346 115 19 -3.222795220394829 115 20 -3.3373726377013178 115 21 0.26071512244912753 115 22 0.9547783445966616 115 23 0.6681916560574166 115 24 1.0346060858898625 115 25 0.4418624790764554 115 26 0.9377927788348046 115 27 1.2672507154860497 115 28 0.9606126982149448 115 29 0.8373736563328151 115 30 0.9233198724358047 115 31 0.3460585793724178 115 32 -7.627238363461053 115 33 0.16257608860755823 115 34 0.2366262578691547 115 35 0.5138470997848543 115 36 2.8627736292083696 115 37 2.0739914873735366 115 38 3.9086449097765055 115 39 5.897823903779166 115 40 8.250172791841141 115 41 7.302650843156314 115 42 1.9391872397972136 115 43 -0.3685503129070007 115 44 -0.66145889335397 115 45 2.2768557519707926 115 46 -4.361364548430302 115 47 -5.429062009478539 115 48 -5.542463098188712 115 49 -9.414636075245541 115 50 -3.355528153052706 115 51 -1.53714817143954 115 52 -0.8526038537629752 115 53 -1.8074657716468892 115 54 -1.34882763040914 115 55 -1.1172869418334948 115 56 1.0013326575031805 116 1 0.5918898477118617 116 2 1.1837479561159796 116 3 1.0007236306464549 116 4 2.024044901907253 116 5 0.7301142346069085 116 6 0.8297511105699229 116 7 0.700867353049837 116 8 0.6465534237832671 116 9 0.4345615248755408 116 10 1.3256423218670221 116 11 -2.156834817434053 116 12 -4.201815450175282 116 13 -6.389933452517866 116 14 -9.00004031543494 116 15 -6.904335657056276 116 16 -8.51278491161808 116 17 -11.897748500446877 116 18 -3.7774140661486584 116 19 -4.208116748819805 116 20 -4.623253345995817 116 21 0.34637837190548026 116 22 0.9124515350284739 116 23 -0.13663903670431665 116 24 0.24422087309085327 116 25 0.3939438708130183 116 26 0.67816245885966 116 27 0.44347003825709663 116 28 1.3309916979042953 116 29 0.4447051257332504 116 30 1.0019933735230993 116 31 0.821206140726872 116 32 -8.380500246033282 116 33 0.1021535971505827 116 34 -0.027741558222800222 116 35 0.4017781342676159 116 36 2.5904840982981483 116 37 2.322049890604358 116 38 3.5022298740511246 116 39 4.756165003499579 116 40 7.315876426510293 116 41 4.9291652886362805 116 42 1.540227935665495 116 43 -9.288168206662828 116 44 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0.7951335454871185 118 9 0.9641423227119148 118 10 0.5345672711570799 118 11 -9.127592085858666 118 12 -13.717535531311185 118 13 -11.899568340955998 118 14 -2.894563642006947 118 15 -18.575228151985282 118 16 -7.848653741908819 118 17 -13.396565268533418 118 18 -4.039753484674691 118 19 -4.123431150442524 118 20 -4.1325501681729655 118 21 -0.26366219604584273 118 22 0.306129695948016 118 23 -0.2176059062107356 118 24 -2.5003700667333497 118 25 0.19093508109023105 118 26 1.2986830809015757 118 27 0.9182841018135477 118 28 0.7926211772822498 118 29 1.5745225699523218 118 30 1.530898038837499 118 31 -1.465812114851218 118 32 -7.910757176391634 118 33 0.1548919338645831 118 34 0.12878312061425135 118 35 -0.5155646649210297 118 36 3.108401610130464 118 37 2.6171817364461383 118 38 3.7366788260457184 118 39 6.205455064351508 118 40 8.701948096557619 118 41 6.241707546918903 118 42 0.24019412903939497 118 43 -1.5134893696107667 118 44 -3.5091006527604343 118 45 0.5564000021032776 118 46 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0.7534054910077401 119 29 1.0657616187633323 119 30 0.8293961614908127 119 31 -1.4539222525233093 119 32 -8.930573613604817 119 33 -0.8626299685133887 119 34 -0.830749628655667 119 35 -0.007429495959826227 119 36 2.0860820440061474 119 37 2.8906485699880697 119 38 4.111730974564701 119 39 5.794316140696939 119 40 7.074497116165464 119 41 6.256934914461663 119 42 0.8705654264945473 119 43 0.5601443391919041 119 44 -0.5028428304825887 119 45 0.994030373400733 119 46 -5.821686572582113 119 47 -9.432991470854809 119 48 -9.198395626461666 119 49 -6.0604095082530165 119 50 -5.450985312339654 119 51 -5.632797011769231 119 52 -5.459370194571871 119 53 -8.646357855219826 119 54 -8.993450977160146 119 55 -9.946307451705067 119 56 -6.732326533602819 120 1 0.3857116867923196 120 2 0.9707450825527602 120 3 0.9292437455889839 120 4 2.53086961455093 120 5 0.7642377978252952 120 6 0.4507999611446394 120 7 0.5632955139693792 120 8 0.6964815847028092 120 9 0.7815442834962312 120 10 0.8629986437060992 120 11 -9.357984242721411 120 12 -3.0962120018994206 120 13 -8.199200693897176 120 14 -7.211965602791263 120 15 -8.19348795590685 120 16 -12.977942957595094 120 17 -12.468869190102048 120 18 -3.6488221121256714 120 19 -3.994754679854289 120 20 -4.7906384034670815 120 21 -1.4039808234968234 120 22 -0.7980369707186554 120 23 -1.5080470826813226 120 24 -6.3398308510470756 120 25 -1.321932565968595 120 26 0.5977026887447181 120 27 1.0972056704409994 120 28 1.0346554910077401 120 29 1.4795470797562444 120 30 0.3777117643276924 120 31 -3.397184663870828 120 32 -10.86541403913673 120 33 -4.035059046527575 120 34 -11.805040408797513 120 35 -3.381986233548478 120 36 -2.4001768212420806 120 37 -1.9606368910048388 120 38 -1.8160172523856524 120 39 -0.47407924937398604 120 40 -3.831752883834536 120 41 -1.3851306883752166 120 42 -5.416668616058644 120 43 -3.7641021147088036 120 44 -5.905546731191807 120 45 -10.294090193975153 120 46 -13.062378061943814 120 47 -11.335030477947006 120 48 -11.633679314404928 120 49 -14.695604543714008 120 50 -9.99376013503469 120 51 -9.878364387655756 120 52 -10.248155655564778 120 53 -10.39192523110635 120 54 -11.73480736013887 120 55 -11.01368333822989 120 56 -7.040615540695017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 801.0 5 56.0 6 143.0 7 146.0 8 272.0 9 390.0 10 332.0 11 226.0 12 259.0 13 312.0 14 370.0 15 496.0 16 743.0 17 981.0 18 1222.0 19 1496.0 20 1866.0 21 2311.0 22 2994.0 23 3563.0 24 4010.0 25 4312.0 26 4424.0 27 4744.0 28 4948.0 29 5162.0 30 5922.0 31 6713.0 32 7982.0 33 9693.0 34 11564.0 35 13352.0 36 15249.0 37 14516.0 38 8262.0 39 2218.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 98.67160386058528 0.9869694687119415 0.13375477821345855 0.2076718924893172 8 98.83703740205982 0.758882373232149 0.11826738284137386 0.2858128418666535 9 95.73061241734794 2.4026307821225115 1.11963157783552 0.7471252226940167 10 58.69018873503179 21.667570098063372 11.748597334760051 7.893643832144794 11 40.98515301058085 28.444410027525713 14.375321191983048 16.195115769910384 12 37.575941035261984 12.25827343700502 19.41345009890814 30.752335428824857 13 27.271895305207284 11.31495026434168 23.1163455378702 38.296808892580835 14 21.966709851856024 12.985129273925534 33.650650594265755 31.397510279952684 15 31.59483670734288 13.998024970021866 26.593073287719545 27.814065034915707 16 38.72412651684286 14.653952066709394 21.866482614850447 24.755438801597307 17 31.903028425373712 18.090986544243457 26.67633659810873 23.3296484322741 18 32.13838160544065 19.537883161318483 26.70900156291801 21.61473367032286 19 35.51769054743883 23.621897553247667 19.00580883647245 21.85460306284105 20 30.53998478916087 29.431565308019493 19.07692741049548 20.95152249232416 21 28.244538164203593 22.378917278622275 28.160049566643902 21.216494990530236 22 26.795683179747833 20.235270927637647 25.989623299002456 26.979422593612064 23 29.168397265770746 19.607042541059197 24.048405150262933 27.176155042907123 24 30.083561537757564 20.58978817466966 26.201857079499618 23.124793208073157 25 35.10102076733544 19.12073213657163 20.03167898627244 25.746568109820483 26 23.991420548351137 17.974515641977227 25.524574202379103 32.50948960729254 27 23.579039289009078 17.257868013155022 25.60018028295974 33.56291241487616 28 23.613805167415517 26.381610038582803 23.227977118351166 26.77660767565051 29 33.8243785648898 22.234349693683544 21.972396310119006 21.968875431307655 30 28.463082285835007 19.460582373860074 30.427801837963454 21.648533502341465 31 28.27191867852605 22.04080192009036 28.356628547225753 21.33065085415784 32 36.605131238030864 16.19282978483722 24.740903458375577 22.461135518756336 33 32.48754604084052 17.63381832037369 26.540692341344325 23.33794329744147 34 24.91156117429848 23.7939199188195 32.76676109537299 18.527757811509026 35 23.732681566213294 25.449909489832574 34.47204750198981 16.345361441964318 36 25.658442309591322 30.530985935361915 25.563925684540184 18.246646070506582 37 26.348040945993645 25.044828803388636 30.020472996823155 18.586657253794563 38 32.73535011635287 23.936957901417387 24.13652069670686 19.19117128552288 39 24.825553033998602 18.240047508960956 22.60846818949854 34.325931267541904 40 17.206662746292768 13.507577421480343 22.997952431222235 46.287807401004656 41 16.881963392451112 16.265205775352435 25.29487835379718 41.55795247839927 42 25.598724226985986 20.13223443740386 29.831072975909905 24.43796835970025 43 14.356652445398806 11.397027660836889 26.736925380725 47.5093945130393 44 16.254306959763976 12.692707706893259 21.629958752237496 49.423026581105276 45 23.604121734962856 19.514808079953763 32.49531300658294 24.385757178500445 46 21.388822360913483 21.022527155154197 27.85463715642214 29.734013327510176 47 19.54491675821854 22.425983830530438 28.68813206005803 29.340967351192994 48 23.175738965694524 25.842142701195165 30.11500890914085 20.867109423969463 49 17.386606020369246 15.578517650050147 20.8657635641942 46.16911276538641 50 26.648223686996285 18.80760490620898 29.798585724569154 24.74558568222558 51 21.817847754190574 26.011250247335838 30.21383950024027 21.957062498233316 52 20.852155839748892 21.16745491435318 34.79106134192983 23.189327903968103 53 22.326090023987582 18.225624382672496 36.62480598278538 22.823479610554536 54 18.6299457927719 20.907495259510934 28.3682144040377 32.09434454367946 55 23.21788670624568 23.261602245004443 29.035578807834955 24.484932240914926 56 25.74786098708481 20.414612297296344 27.550063129977215 26.287463585641635 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 3.0 9 5.0 10 15.0 11 25.0 12 66.5 13 126.5 14 145.0 15 167.0 16 189.0 17 235.5 18 282.0 19 337.0 20 392.0 21 491.0 22 678.5 23 767.0 24 872.0 25 977.0 26 1188.0 27 1399.0 28 1652.5 29 2126.5 30 2347.0 31 2488.0 32 2629.0 33 2586.0 34 2543.0 35 2424.0 36 2305.0 37 2088.0 38 1819.5 39 1768.0 40 1726.5 41 1685.0 42 1797.0 43 1909.0 44 2042.5 45 2176.0 46 3007.5 47 4810.0 48 5781.0 49 5440.5 50 5100.0 51 5014.0 52 4928.0 53 5213.0 54 5951.0 55 6404.0 56 6939.0 57 7474.0 58 8555.0 59 9636.0 60 11105.0 61 12574.0 62 12683.5 63 11781.0 64 10769.0 65 9624.0 66 8479.0 67 7610.5 68 6742.0 69 5967.5 70 5193.0 71 4596.5 72 3475.5 73 2951.0 74 2381.5 75 1812.0 76 1427.0 77 1042.0 78 794.0 79 412.0 80 278.0 81 200.0 82 122.0 83 86.5 84 51.0 85 32.0 86 13.0 87 7.5 88 2.5 89 3.0 90 2.5 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02815890067651759 10 0.0 11 0.0014079450338258795 12 0.0 13 0.0 14 0.019007257956649374 15 0.19781627725253603 16 0.04364629604860226 17 0.02111917550738819 18 0.006335752652216457 19 0.01830328543973643 20 0.03308670829490817 21 0.014079450338258795 22 0.0014079450338258795 23 0.0 24 0.0 25 7.039725169129397E-4 26 0.22315928786140188 27 0.03660657087947286 28 0.19570435970179723 29 0.02886287319343053 30 0.03238273577799523 31 0.2752532541129594 32 0.013375477821345856 33 0.2316069580643572 34 0.10207601495237627 35 0.05490985631920929 36 0.1950003871848843 37 0.28229297928208885 38 0.16965737657601848 39 0.4251994002154156 40 0.6392070453569493 41 0.9257238597405157 42 0.23442284813200892 43 0.3372028356012981 44 0.4998204870081872 45 0.11897135535828682 46 0.06265355400525163 47 0.03871848843021168 48 0.04364629604860226 49 0.32734722036451697 50 0.24850229847026775 51 0.38225707668372627 52 0.4209755651139379 53 0.21893545275992427 54 0.13234683317963267 55 0.15839381630541144 56 0.19711230473562313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 142051.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.4368106203708 #Duplication Level Percentage of deduplicated Percentage of total 1 91.74292744305689 71.96022625609473 2 5.143978346664486 8.069545108252209 3 1.3742804928701877 3.2338253627558635 4 0.5756819294889342 1.8061861792357279 5 0.2917872698191141 1.1443431412118448 6 0.17686733925967718 0.8323745996664106 7 0.12190825729029224 0.6693466423096665 8 0.0999247714004517 0.627022429649686 9 0.0669495944752928 0.472618139667225 >10 0.3607284103457655 4.924477209912053 >50 0.024981191849392476 1.3731790659246936 >100 0.015987962783611184 2.8811679488237294 >500 0.003996990695902796 2.0056879164961705 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 812 0.571625683733307 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 677 0.47658939395006017 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 555 0.39070474688668155 No Hit TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 515 0.36254584621016395 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 410 0.28862873193430527 No Hit TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 403 0.2837009243159147 No Hit TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 368 0.2590618862239618 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 337 0.23723873819966068 No Hit TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 290 0.20415202990475254 No Hit TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 278 0.19570435970179723 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 258 0.18162490936353845 No Hit TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 197 0.13868258583184914 No Hit TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC 193 0.13586669576419735 No Hit TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC 170 0.11967532787519974 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 158 0.11122765767224446 No Hit TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTC 148 0.10418793250311507 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 7.039725169129397E-4 0.0 0.0 0.0 0.0 9 0.0014079450338258795 0.0 0.0 0.0 0.0 10 0.004223835101477638 0.0 0.0 0.0 0.0 11 0.0077436976860423366 0.0 0.0 0.0 0.0 12 0.009151642719868217 0.0 0.0 0.0 0.0 13 0.009855615236781155 0.0 0.0 0.0 0.0 14 0.011967532787519976 0.0 0.0 0.0 0.0 15 0.013375477821345854 0.0 0.0 0.0 0.0 16 0.014079450338258795 0.0 0.0 0.0 0.0 17 0.017599312922823494 0.0 0.0 0.0 0.0 18 0.02252712054121407 0.0 0.0 0.0 0.0 19 0.02956684571034347 0.0 0.0 0.0 0.0 20 0.03238273577799523 0.0 0.0 0.0 0.0 21 0.03801451591329875 0.0 0.0 0.0 0.0 22 0.03942246094712462 0.0 0.0 0.0 0.0 23 0.04223835101477638 0.0 0.0 0.0 0.0 24 0.04294232353168932 0.0 0.0 0.0 0.0 25 0.04505424108242814 0.0 0.0 0.0 0.0 26 0.04646218611625402 0.0 0.0 0.0 0.0 27 0.04998204870081872 0.0 0.0 0.0 0.0 28 0.0549098563192093 0.0 0.0 0.0 0.0 29 0.05631780135303518 0.0 0.0 0.0 0.0 30 0.058429718903774 0.0 0.0 0.0 0.0 31 0.058429718903774 0.0 0.0 0.0 0.0 32 0.058429718903774 0.0 0.0 0.0 0.0 33 0.058429718903774 0.0 0.0 0.0 0.0 34 0.058429718903774 0.0 0.0 0.0 0.0 35 0.058429718903774 0.0 0.0 0.0 0.0 36 0.058429718903774 0.0 0.0 0.0 0.0 37 0.05913369142068694 0.0 0.0 0.0 0.0 38 0.05913369142068694 0.0 0.0 0.0 0.0 39 0.05983766393759988 0.0 0.0 0.0 0.0 40 0.05983766393759988 0.0 0.0 0.0 0.0 41 0.06054163645451282 0.0 0.0 0.0 0.0 42 0.06054163645451282 0.0 0.0 0.0 0.0 43 0.06124560897142575 0.0 0.0 0.0 0.0 44 0.06687738910672927 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTAAT 20 3.8871242E-4 50.719727 40 AGACTAC 15 0.008032736 50.592472 37 ACCCTAC 15 0.008032736 50.592472 41 TCTGAAA 15 0.008124212 50.447815 36 TGATACC 20 4.062617E-4 50.26816 34 ATATGCA 25 2.0133199E-5 50.26816 34 TAAAGTG 15 0.008239644 50.268158 34 TAGGATC 15 0.008239644 50.268158 34 AGATAGA 15 0.0082628755 50.232384 49 CGATTCA 75 0.0 50.232384 49 CGATTAA 135 0.0 50.232384 49 ACGATTA 135 0.0 50.19666 48 ACGATTC 80 0.0 50.196655 48 GTTCGCG 15 0.008286157 50.196655 48 TCATATG 15 0.008286157 50.196655 48 CTCATAT 15 0.008297815 50.17881 47 AACGATT 160 0.0 50.17881 47 CCCTACT 15 0.008309486 50.16098 42 TCCCACA 15 0.008309486 50.16098 42 TTGTGCA 15 0.008391524 50.03651 50 >>END_MODULE