FastQCFastQC Report
Fri 17 Jun 2016
SRR1526342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40629
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1590.39134608284722733No Hit
TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG1470.3618105294247951No Hit
TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG1380.3396588643579709No Hit
TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1360.3347362721208989No Hit
TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1240.30520071869846666No Hit
TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG1230.3027394225799306No Hit
TGTGCGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC630.15506165546776932No Hit
TGTGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA590.14521647099362525No Hit
TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG530.13044869428240913No Hit
TGTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG490.12060350980826504No Hit
TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG460.11321962145265697No Hit
TGTGCGGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG450.11075832533412094No Hit
TGTGCGGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG450.11075832533412094No Hit
TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG410.10091314085997688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACC150.00798746150.5982538
TTCGTTT150.00798746150.5982538
CCAGATG150.00798746150.5982540
TCCGCCC309.4193456E-750.5982538
CCCTCCG150.00798746150.5982539
TCCGACC150.00798746150.5982538
TACTCAC150.00798746150.5982538
CAAACCA150.00798746150.5982539
CCGACCA150.00798746150.5982539
CGACCAG150.00798746150.5982540
GACCAGT150.00802712350.53541
AAGAGCA251.9693067E-550.34620735
ACAGAAT150.00826818350.1588142
TAGTTCT150.00826818350.1588144
TCTTCTC150.00826818350.1588143
CCAACAC402.5011104E-950.15880643
ACCAGTT204.07144E-450.15880642
CTCTGAG150.00830887750.09665747
GTTCTTA150.00830887750.09665746
TCTATTT150.00830887750.09665746