##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526342_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40629 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.24635112850427 39.0 38.0 40.0 35.0 40.0 2 37.63860789091535 39.0 38.0 40.0 35.0 40.0 3 37.971079770607204 39.0 38.0 40.0 35.0 40.0 4 37.69019665755987 39.0 38.0 40.0 35.0 40.0 5 37.73093110832164 39.0 38.0 40.0 35.0 40.0 6 37.42225996209604 38.0 38.0 39.0 35.0 40.0 7 37.252652046567725 38.0 38.0 39.0 34.0 40.0 8 37.253808855743436 38.0 38.0 39.0 34.0 40.0 9 36.39471805852962 38.0 35.0 39.0 32.0 40.0 10 36.12756897782372 38.0 35.0 39.0 31.0 40.0 11 34.67158925890374 38.0 32.0 39.0 27.0 40.0 12 33.096901228186766 37.0 31.0 39.0 19.0 40.0 13 32.842280144724214 37.0 31.0 39.0 18.0 40.0 14 32.276255876344486 36.0 29.0 39.0 16.0 40.0 15 32.93918137291097 37.0 30.0 39.0 18.0 40.0 16 32.69507002387457 36.0 30.0 39.0 18.0 40.0 17 32.949863398065425 36.0 30.0 39.0 19.0 40.0 18 33.07349430209948 36.0 30.0 39.0 20.0 40.0 19 33.88052868640626 37.0 32.0 39.0 25.0 40.0 20 34.00794998646287 37.0 32.0 39.0 25.0 40.0 21 35.48448152797263 38.0 35.0 39.0 28.0 40.0 22 35.14081075094145 38.0 35.0 39.0 27.0 40.0 23 35.14337049890472 38.0 35.0 39.0 27.0 40.0 24 34.85744172881439 38.0 34.0 39.0 26.0 40.0 25 35.01649068399419 38.0 35.0 39.0 27.0 40.0 26 35.38666962022201 38.0 35.0 39.0 28.0 40.0 27 35.49016712200645 38.0 35.0 39.0 29.0 40.0 28 35.22877747421792 38.0 35.0 39.0 28.0 40.0 29 34.98597061212435 38.0 35.0 39.0 27.0 40.0 30 34.85884466760196 38.0 35.0 39.0 27.0 40.0 31 34.63698343547712 38.0 35.0 39.0 26.0 40.0 32 33.55327475448571 38.0 33.0 39.0 19.0 40.0 33 34.72431022176278 38.0 35.0 39.0 26.0 40.0 34 34.62128036624086 38.0 35.0 39.0 26.0 40.0 35 34.67176154963204 38.0 35.0 39.0 27.0 40.0 36 32.58325334120948 36.0 31.0 38.0 22.0 39.0 37 32.30495458908661 35.0 31.0 38.0 20.0 39.0 38 31.272539319205492 35.0 30.0 38.0 15.0 39.0 39 29.525535947229812 34.0 27.0 38.0 6.0 39.0 40 25.470599817864088 31.0 12.0 37.0 4.0 39.0 41 21.914789928376283 26.0 9.0 33.0 4.0 37.0 42 25.756602426837972 30.0 21.0 34.0 4.0 37.0 43 22.084643973516453 26.0 9.0 33.0 4.0 37.0 44 23.155775431342146 28.0 10.0 33.0 4.0 37.0 45 25.610130694823894 30.0 21.0 34.0 4.0 37.0 46 25.926973344163038 31.0 18.0 36.0 4.0 38.0 47 25.01868123753969 30.0 11.0 36.0 4.0 38.0 48 24.957788771567106 30.0 13.0 36.0 4.0 38.0 49 22.564621329592164 28.0 5.0 35.0 4.0 38.0 50 24.817396440965812 30.0 11.0 35.0 4.0 38.0 51 26.260257451574 33.0 4.0 38.0 4.0 40.0 52 25.966083339486573 32.0 4.0 38.0 4.0 40.0 53 25.639100150139065 32.0 4.0 38.0 4.0 40.0 54 24.701444780821582 31.0 4.0 38.0 4.0 39.0 55 24.910187304634622 31.0 4.0 38.0 4.0 39.0 56 24.460828472273498 32.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6832549890894413 1 2 -0.031467338788935706 1 3 -0.4633949558389361 1 4 -0.22675016301431583 1 5 -0.0996667284343431 1 6 0.13690211335184443 1 7 0.6772580077884669 1 8 0.6546784105082679 1 9 0.4619719498251271 1 10 0.12353084693207705 1 11 -4.694632504220415 1 12 -7.3936293753603195 1 13 -21.830157694227687 1 14 -17.472924367809874 1 15 -13.392382640683717 1 16 -22.328950849623332 1 17 -21.71432353085524 1 18 -19.846037132894597 1 19 -6.25020392375108 1 20 -11.279564861905971 1 21 -9.882134055544821 1 22 -2.833210461468198 1 23 -2.691418477944982 1 24 -3.360520726743843 1 25 -3.7032426355273316 1 26 2.367289924737989 1 27 1.9299322256608278 1 28 2.029067305343979 1 29 0.6556621387440842 1 30 1.8183406189809972 1 31 0.996641718552219 1 32 -12.248225309368607 1 33 -0.1973079782631828 1 34 0.48517990667251354 1 35 0.39689064820936437 1 36 2.97287298417665 1 37 3.318049183653226 1 38 3.490904212901995 1 39 3.9188747167309934 1 40 5.907679752201517 1 41 -3.0806865858897226 1 42 -8.157454947847846 1 43 -13.191436377909241 1 44 -15.857170222858613 1 45 -13.743807963768749 1 46 -17.808238391372765 1 47 -17.89477004298505 1 48 -14.89387765523444 1 49 -13.90343925735392 1 50 -14.421989869278594 1 51 -15.899394148666415 1 52 -16.56072082909029 1 53 -16.89513908567384 1 54 -17.756835200273038 1 55 -15.933012361598298 1 56 -15.060256194811446 2 1 -0.4731100615532142 2 2 0.2747874285642311 2 3 -0.2547755813156698 2 4 -0.101654815951008 2 5 0.2016299916266746 2 6 0.8317915107584 2 7 0.8256180382995311 2 8 0.7896898521558668 2 9 0.6305455577579977 2 10 0.5766200917833402 2 11 -3.7964631678359737 2 12 -11.009571404345827 2 13 -13.042972339536611 2 14 -15.577806137451367 2 15 -10.072397896213847 2 16 -3.957097302712576 2 17 -2.4469703653327386 2 18 -10.770903647005962 2 19 -2.0000132296244573 2 20 -3.557596898519243 2 21 1.4496737247755433 2 22 2.1698406445577376 2 23 2.125133772245711 2 24 1.8721261077336564 2 25 2.1510670517343016 2 26 2.4542464464771214 2 27 2.140839929703546 2 28 1.472240455610951 2 29 2.6015050067837464 2 30 1.160064493885649 2 31 -1.0079349404866846 2 32 -8.137241327675241 2 33 -1.6111142330305377 2 34 -0.6803425952344284 2 35 -0.9658095806235849 2 36 3.0952986134672678 2 37 1.9976830509301102 2 38 2.674351962711306 2 39 4.944046341444949 2 40 7.39585671635102 2 41 4.910541484285716 2 42 0.33987533437946027 2 43 -3.020955828710157 2 44 -9.60011454017364 2 45 -1.3402991918389233 2 46 -12.1507250427839 2 47 -12.350910393862245 2 48 -8.75581510756091 2 49 -12.042645969787177 2 50 -8.454789259057389 2 51 -7.973002081542081 2 52 -7.498173155558636 2 53 -7.905436568511368 2 54 -14.395660524453055 2 55 -7.3319444744892195 2 56 -6.3203629835223545 3 1 0.38261133148161264 3 2 1.1823548545919849 3 3 0.8808620201506798 3 4 1.0198429583354596 3 5 1.0599698711762997 3 6 1.4418988687055077 3 7 1.9060055743037196 3 8 1.7260606298463586 3 9 2.468461242492225 3 10 2.1280733517990456 3 11 1.5782422524311102 3 12 2.607344018539745 3 13 -3.7334672334879073 3 14 -5.80744740479884 3 15 -2.893031569950427 3 16 2.720043310013267 3 17 2.7402514204750616 3 18 2.2827753265473234 3 19 0.48178828909772164 3 20 1.1399679090219976 3 21 3.2619282416647835 3 22 3.236873899336473 3 23 3.7813264928532035 3 24 3.7446058144631635 3 25 3.338288837542102 3 26 3.379619580805482 3 27 3.028245009567385 3 28 2.8953878764017347 3 29 3.396739361326823 3 30 3.0558487305968285 3 31 3.9064405504795374 3 32 2.8831828671401567 3 33 3.8945155129762696 3 34 2.9965361688399383 3 35 3.7521794217330537 3 36 5.0835154241506935 3 37 6.048743537968601 3 38 6.242039839118739 3 39 7.959757260533717 3 40 10.68834166005356 3 41 9.479014906647592 3 42 7.475251087194334 3 43 1.9633332522010782 3 44 4.674302846576818 3 45 6.494998006380506 3 46 1.4143443471087416 3 47 -3.2082028788059453 3 48 1.7401262383411584 3 49 -11.026411353395451 3 50 0.8693793714741638 3 51 0.20086542304026977 3 52 0.2342175226293719 3 53 -0.31077828100155 3 54 -5.490449736288614 3 55 -0.633855969645019 3 56 3.0695296585305414 4 1 0.24378491561574123 4 2 0.5554891829501969 4 3 0.007421006043138334 4 4 0.8000515046450047 4 5 0.4292195133762604 4 6 0.9332094391410024 4 7 1.4833926117193812 4 8 1.23597680240821 4 9 1.8747176317786725 4 10 1.53985008457353 4 11 2.7702275315151397 4 12 3.145679737206681 4 13 4.430711870989704 4 14 3.4219535357041266 4 15 5.45127429797024 4 16 5.738648912139624 4 17 5.403306010538444 4 18 5.43121122922571 4 19 4.200659685540167 4 20 3.518827038034466 4 21 2.949313234508786 4 22 3.271018246095828 4 23 2.6257345893569877 4 24 3.6519867181638404 4 25 2.782165345413709 4 26 2.6186300081209595 4 27 2.4823443757501664 4 28 1.1785610516215286 4 29 2.4186162781127507 4 30 1.8344216195765881 4 31 3.271805506521247 4 32 3.2845322844372333 4 33 1.2574269830232936 4 34 1.710909507625459 4 35 0.6556756392754792 4 36 3.310871792991417 4 37 4.332938999019515 4 38 4.255942926421952 4 39 6.241090321257499 4 40 8.721259263815575 4 41 7.18643670178151 4 42 8.434564111115822 4 43 7.188849506545793 4 44 10.619303868862648 4 45 7.256192036231251 4 46 6.290034390044745 4 47 6.447695710439607 4 48 5.380690540119936 4 49 3.699002672366152 4 50 6.234948784682096 4 51 5.238485541625426 4 52 5.801995073232522 4 53 5.039047930407161 4 54 1.2541832630980139 4 55 6.3671663161861005 4 56 5.756301279875867 5 1 0.5435566051134515 5 2 0.7888225162835312 5 3 0.5873296818422276 5 4 0.9623802717682963 5 5 0.5191738512758022 5 6 1.069510809004015 5 7 1.2642145295275995 5 8 1.4133740626821805 5 9 1.877913978810632 5 10 1.238023600555266 5 11 4.914063147953499 5 12 6.407095262320841 5 13 6.530711870989705 5 14 6.583597371320565 5 15 6.079356489751067 5 16 6.396411469217249 5 17 5.957415599579541 5 18 6.472078809134388 5 19 4.1113902791474715 5 20 4.9994206453404075 5 21 2.8584456546001107 5 22 3.235630118241943 5 23 3.0549583336492248 5 24 3.9177401428213727 5 25 2.805453016646581 5 26 2.9542464464771214 5 27 2.436453964791262 5 28 1.7739948415758633 5 29 2.8129085155556766 5 30 2.6451522131838914 5 31 3.151714182320333 5 32 4.032934110921246 5 33 3.0932717318817424 5 34 3.121411790730484 5 35 3.3947167351658862 5 36 4.499684578379547 5 37 3.7064549807546747 5 38 5.444527401307795 5 39 6.58264283267302 5 40 5.572172505824707 5 41 2.1193134141102767 5 42 8.063559544905779 5 43 10.207114346728439 5 44 10.788481951054429 5 45 8.389525369564584 5 46 9.751906536163467 5 47 10.238563290348281 5 48 9.566991909982953 5 49 10.666125960037384 5 50 9.683807232170683 5 51 8.158576865826337 5 52 7.708844388301014 5 53 9.412107291137755 5 54 7.7859184229153655 5 55 9.511915174633586 5 56 8.041917718232032 6 1 0.6062550178118684 6 2 1.1904098178708296 6 3 0.6984407929533347 6 4 1.1314278908159139 6 5 0.23504686714881728 6 6 1.6814155709087757 6 7 1.7562780215910934 6 8 1.8982946976028146 6 9 2.435056835953489 6 10 1.9316743942060626 6 11 3.874380608270961 6 12 6.768206373431948 6 13 5.525949966227799 6 14 7.0470894348126265 6 15 5.788086648481222 6 16 5.430538453344234 6 17 5.7677330598970045 6 18 5.088745475801055 6 19 4.339168056925253 6 20 3.6938650897848504 6 21 2.4822551784096305 6 22 2.945947578559405 6 23 2.9279742066650982 6 24 3.2129782380594705 6 25 2.6514847626783293 6 26 2.3113893036199755 6 27 1.9102634886007905 6 28 2.634312301893324 6 29 2.4803688330159943 6 30 1.9888030068346865 6 31 2.3279046585108105 6 32 2.9488071267942644 6 33 2.063906652516664 6 34 2.688078457397154 6 35 1.9272564177055713 6 36 3.468732197427162 6 37 3.1889946632943555 6 38 3.633416290196685 6 39 4.747722197752385 6 40 2.620585204237404 6 41 2.8637578585547203 6 42 5.258003989350222 6 43 9.730923870537964 6 44 8.505148617721098 6 45 5.700636480675694 6 46 9.01301764727458 6 47 8.454436306221297 6 48 8.414610957601997 6 49 10.799459293370717 6 50 8.571108819472268 6 51 7.7379419451914195 6 52 7.499320578777205 6 53 8.112107291137754 6 54 8.172426359423305 6 55 7.6722326349510475 6 56 7.9411240674383805 7 1 0.7222922372973599 7 2 0.6589374588122681 7 3 0.7367549691985502 7 4 0.650311306251055 7 5 1.1042313225401656 7 6 1.0229590848660877 7 7 1.6090421157344963 7 8 1.7461326833718402 7 9 2.0032013351324736 7 10 2.2472190028541164 7 11 4.224407975539705 7 12 5.944451584159918 7 13 6.258298077886259 7 14 6.483597371320563 7 15 6.560390972509683 7 16 6.296986181860923 7 17 5.807415599579542 7 18 6.0542627171803645 7 19 4.814263842365861 7 20 4.674708001662246 7 21 2.5676410568989567 7 22 3.353446210195969 7 23 3.049211207212444 7 24 3.7384297979937884 7 25 3.1123495683707176 7 26 2.6783843775116054 7 27 1.834729826860233 7 28 2.196983347322991 7 29 1.9675062167051038 7 30 2.568715431574695 7 31 2.9666567110559683 7 32 3.5996007775879164 7 33 2.390398168663353 7 34 2.6823313309603734 7 35 2.8654063903383005 7 36 0.6370409002186257 7 37 1.150133141674214 7 38 1.7157917691238858 7 39 2.5148267407189984 7 40 -1.8651838160143726 7 41 -8.71861762037248 7 42 4.646892878239111 7 43 9.477229289257174 7 44 7.355723330364775 7 45 5.342398932782974 7 46 8.062826076393353 7 47 8.312126508739087 7 48 7.625612599638121 7 49 9.276470787623591 7 50 7.627485393090222 7 51 8.612599854332085 7 52 8.308844388301015 7 53 9.468429130218215 7 54 9.586493135559042 7 55 9.786627818311747 7 56 9.040193580300997 8 1 0.7820982717801215 8 2 1.336739182950197 8 3 0.7227463485088919 8 4 1.1998802717682935 8 5 1.0368821846091336 8 6 1.4976358090040165 8 7 1.7173395295275995 8 8 1.9508740626821819 8 9 2.588330645477299 8 10 1.7994819338886003 8 11 4.710938147953499 8 12 5.980011928987505 8 13 6.711961870989704 8 14 6.780472371320563 8 15 6.613731489751064 8 16 6.4672448025505815 8 17 5.604290599579542 8 18 5.932495475801055 8 19 4.978056945814139 8 20 4.525462312007072 8 21 3.1042789879334407 8 22 3.7095884515752786 8 23 3.596625000315889 8 24 3.6646151428213756 8 25 2.4835780166465824 8 26 1.9229964464771214 8 27 2.792703964791265 8 28 2.2729531749091976 8 29 2.8139501822223423 8 30 2.29931887985056 8 31 3.0340058489870003 8 32 4.208975777587916 8 33 3.2891050652150753 8 34 3.276620124063818 8 35 3.6665917351658877 8 36 0.9069762450462129 8 37 1.6835383140880076 8 38 2.3122357346411277 8 39 3.2055594993396888 8 40 -1.4007441608419597 8 41 -9.877561585889723 8 42 5.381267878239111 8 43 9.826906013395106 8 44 8.44785695105443 8 45 6.099942036231251 8 46 8.844614869496802 8 47 8.995854957014949 8 48 8.64824190998295 8 49 10.622375960037385 8 50 8.770265565504015 8 51 9.773160199159673 8 52 9.918219388301015 8 53 9.763148957804422 8 54 9.4442517562487 8 55 8.935873507966921 8 56 9.232542718232033 9 1 -0.0018456937371169602 9 2 0.6934202174329585 9 3 0.1160653140261303 9 4 0.7365182028027775 9 5 0.7249209777125785 9 6 0.8505452917626357 9 7 1.126283495044838 9 8 1.1771671661304595 9 9 1.2273392661669575 9 10 2.212736244233426 9 11 4.120959699677634 9 12 6.0651412393323305 9 13 5.792780836506942 9 14 6.552562888561944 9 15 5.956942696647619 9 16 6.14181376806782 9 17 5.462588013372645 9 18 5.2611592689045 9 19 4.486677635469313 9 20 4.209190760282937 9 21 2.171089332761028 9 22 2.4396531067476914 9 23 2.842314655488302 9 24 3.5142918669593044 9 25 2.267521982163821 9 26 2.419763687856431 9 27 2.5416263785843682 9 28 0.765948864564372 9 29 2.0881958718775167 9 30 2.1549223281264247 9 31 2.587346366228381 9 32 3.513393881036194 9 33 2.166260237628869 9 34 2.2857796068224374 9 35 1.106785700683126 9 36 0.7749719347013873 9 37 0.92599521063973 9 38 1.4226883208480245 9 39 2.7734474303741727 9 40 -2.7444941608419597 9 41 -4.9427555514069645 9 42 2.9744790851356626 9 43 7.6496430823606225 9 44 7.0453785027785685 9 45 4.342398932782974 9 46 7.44213642122094 9 47 8.122471336325294 9 48 7.884233289293295 9 49 8.535091477278765 9 50 7.317140565504015 9 51 7.940186061228637 9 52 8.308844388301015 9 53 7.9856705095285605 9 54 8.741665549352149 9 55 7.562489887277266 9 56 5.97122806305962 10 1 -0.08523127367442385 10 2 0.5736710011320127 10 3 0.47217816669071055 10 4 0.9669257263137467 10 5 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1.280671049989742 114 35 0.6150871055362614 114 36 1.590425319120289 114 37 3.2471957214954124 114 38 3.226008882789273 114 39 6.123383573413765 114 40 8.848098431750635 114 41 8.308202302999167 114 42 2.9246706560168896 114 43 -0.2743671347530423 114 44 -2.7170736045011274 114 45 1.330266110305324 114 46 -4.653649019392088 114 47 -12.41328856150357 114 48 -8.410785867794829 114 49 -14.867207373295948 114 50 -7.349526101162651 114 51 -8.637719430469957 114 52 -8.209674130217504 114 53 -8.369374190343727 114 54 -10.949266762269819 114 55 -8.639936677218264 114 56 -6.037711911397597 115 1 -0.3272767282198785 115 2 0.08673918295019689 115 3 -0.30850365149110814 115 4 -0.4251197282317065 115 5 -0.6037428153908664 115 6 -0.2679891909959835 115 7 0.1079645295275995 115 8 0.1696240626821819 115 9 -0.2554193545227008 115 10 -1.2473930661113997 115 11 -1.2421868520465011 115 12 -2.832488071012495 115 13 -2.319288129010296 115 14 -6.047652628679437 115 15 -1.7143935102489358 115 16 -8.032755197449418 115 17 -11.255084400420458 115 18 -1.0050045241989451 115 19 -1.5219430541858614 115 20 -2.146412687992928 115 21 -0.020721012066559297 115 22 0.3814634515752786 115 23 -0.37212499968411095 115 24 0.8677401428213756 115 25 0.4679530166465824 115 26 0.3604964464771214 115 27 1.167703964791265 115 28 2.0385781749091976 115 29 1.5795751822223423 115 30 0.5493188798505599 115 31 -1.1691191510129997 115 32 -2.8066492224120836 115 33 -0.3671449347849247 115 34 0.1359951240638182 115 35 -0.0052832648341123445 115 36 1.1101012450462129 115 37 1.2772883140880076 115 38 2.7028607346411277 115 39 3.7055594993396888 115 40 6.75550583915804 115 41 8.669313414110277 115 42 4.115642878239111 115 43 7.0300310133951065 115 44 2.9009819510544297 115 45 4.631192036231251 115 46 0.5477398694968016 115 47 -4.863520042985051 115 48 -4.41425809001705 115 49 -5.846374039962615 115 50 1.3796405655040154 115 51 1.8044101991596726 115 52 1.433844388301015 115 53 -1.0024760421955783 115 54 -0.1182482437512995 115 55 0.7327485079669209 115 56 3.9044177182320325 116 1 -0.11199895044209995 116 2 0.6388225162835326 116 3 0.4762185707311133 116 4 0.1512691606571792 116 5 0.6358405179424693 116 6 0.14173303122623793 116 7 1.1531034184164852 116 8 0.8467073960155176 116 9 1.1056917565884135 116 10 0.2491347116663789 116 11 -0.4470479631576154 116 12 0.04598415120972632 116 13 -3.0415103512325175 116 14 -7.099735962012769 116 15 -2.3984212880267144 116 16 -8.859144086338308 116 17 -12.331473289309347 116 18 -3.3834767464211666 116 19 -4.3274986097414185 116 20 -3.8616904657707067 116 21 0.652890099044555 116 22 1.7911856737975 116 23 0.8327361114270033 116 24 2.089962365043597 116 25 0.8776752388688038 116 26 0.9820242242549 116 27 1.580898409235708 116 28 1.0073281749091976 116 29 1.2740196266667851 116 30 1.0007077687394457 116 31 -0.4816191510129997 116 32 -8.844843666856526 116 33 0.03216062077063242 116 34 -1.1730326537139604 116 35 -0.9219499315007766 116 36 0.57190680060177 116 37 2.3397883140880076 116 38 2.1611940679744635 116 39 3.2437539437841316 116 40 6.061061394713597 116 41 3.1137578585547203 116 42 0.8135595449057789 116 43 -11.54288565327156 116 44 -2.8004069378344596 116 45 0.1450809251201406 116 46 -4.931426797169866 116 47 -9.866992265207273 116 48 -7.549674756683716 116 49 -6.367207373295949 116 50 -3.5995261011626525 116 51 -3.0080898008403274 116 52 -3.274488945032317 116 53 -4.332337153306689 116 54 -9.014081577084633 116 55 -5.038084825366411 116 56 -2.6580822817679675 117 1 -0.7897767282198771 117 2 -1.2945108170498045 117 3 -0.5460036514911053 117 4 -0.6876197282317094 117 5 -0.4474928153908664 117 6 -0.18048919099598493 117 7 -0.13578547047239908 117 8 -0.8366259373178195 117 9 -1.1054193545227022 117 10 -1.628643066111401 117 11 -7.7859368520465 117 12 -9.176238071012495 117 13 -11.419288129010297 117 14 -8.666402628679435 117 15 -4.120643510248936 117 16 -12.27025519744942 117 17 -7.4925844004204585 117 18 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118 38 2.185003591783982 118 39 3.513595213625404 118 40 6.00550583915804 118 41 6.205027699824562 118 42 0.646892878239111 118 43 0.5523524419665335 118 44 0.6956248081972873 118 45 0.43476346480267836 118 46 -5.657617273360341 118 47 -8.501912900127909 118 48 -9.525865232874192 118 49 -4.855302611391187 118 50 -5.040002291638842 118 51 -5.5795183722689 118 52 -4.262584183127558 118 53 -5.614083185052721 118 54 -9.1807482437513 118 55 -6.7404657777473655 118 56 -4.97951085319654 119 1 0.06855660511345718 119 2 0.4721558496168612 119 3 0.42066301517555615 119 4 0.3457136051016292 119 5 0.3441738512757979 119 6 0.4195108090040165 119 7 0.7642145295275995 119 8 0.5967073960155176 119 9 0.2862473121439635 119 10 0.05469026722193604 119 11 -7.252603518713169 119 12 -5.926238071012495 119 13 -1.1526214623436282 119 14 -3.1830692953461046 119 15 -4.287310176915604 119 16 -10.011921864116086 119 17 -10.40091773375379 119 18 -1.452921190865613 119 19 -0.8552763875191935 119 20 -1.8339126879929282 119 21 -1.041554345399895 119 22 0.6522967849086143 119 23 0.13829166698255335 119 24 -3.7572598571786244 119 25 -0.2195469833534176 119 26 0.5375797798104571 119 27 1.261453964791265 119 28 1.1739948415758619 119 29 0.912908515555678 119 30 1.5284855465172242 119 31 -2.9816191510129997 119 32 -7.150399222412084 119 33 -0.06506160145158901 119 34 -0.9785882092695175 119 35 0.20305006849922336 119 36 3.308017911712877 119 37 4.089788314088008 119 38 4.952860734641128 119 39 6.840976166006353 119 40 8.50550583915804 119 41 7.87764674744361 119 42 3.563559544905779 119 43 -3.0012189866048935 119 44 -4.1198513822789025 119 45 -0.07714129710208084 119 46 -3.8480934638365305 119 47 -9.061436709651717 119 48 -8.466341423350384 119 49 -6.283874039962615 119 50 -2.2245261011626525 119 51 -4.424756467506995 119 52 -6.941155611698985 119 53 -10.971226042195578 119 54 -12.1807482437513 119 55 -12.454751492033079 119 56 -9.074748948434634 120 1 -0.6197767282198754 120 2 -0.16451081704980197 120 3 -0.3860036514911087 120 4 0.03238027176829661 120 5 -0.24749281539086354 120 6 -0.1604891909959818 120 7 -0.05578547047240079 120 8 0.06337406268217904 120 9 -1.6354193545227034 120 10 -1.6886430661113963 120 11 -13.9859368520465 120 12 -4.0362380710124945 120 13 -9.969288129010295 120 14 -9.616402628679438 120 15 -8.170643510248937 120 16 -9.860255197449419 120 17 -9.532584400420458 120 18 -2.031254524198946 120 19 -2.6519430541858604 120 20 -4.563912687992929 120 21 0.33177898793343985 120 22 0.7789634515752795 120 23 0.6416250003158908 120 24 -4.202259857178625 120 25 0.545453016646583 120 26 -0.6257535535228769 120 27 1.346453964791266 120 28 1.347328174909201 120 29 1.3895751822223446 120 30 1.901818879850559 120 31 -2.921619151013001 120 32 -13.400399222412084 120 33 -3.563394934784924 120 34 -7.645254875936182 120 35 -2.9552832648341116 120 36 -0.08364875495378854 120 37 -1.4002116859119909 120 38 -1.882139265358873 120 39 0.24930949933968805 120 40 -0.30449416084195846 120 41 -5.700686585889722 120 42 -11.65310712176089 120 43 -8.926218986604894 120 44 -9.48151804894557 120 45 -14.183807963768748 120 46 -15.564760130503199 120 47 -14.854770042985052 120 48 -13.11300809001705 120 49 -16.133874039962613 120 50 -12.402859434495985 120 51 -11.568089800840328 120 52 -11.971155611698984 120 53 -12.381226042195578 120 54 -14.8707482437513 120 55 -11.41475149203308 120 56 -9.728082281767968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 89.0 5 31.0 6 62.0 7 60.0 8 77.0 9 124.0 10 58.0 11 73.0 12 59.0 13 79.0 14 110.0 15 119.0 16 158.0 17 260.0 18 283.0 19 399.0 20 466.0 21 634.0 22 791.0 23 926.0 24 1084.0 25 1247.0 26 1254.0 27 1395.0 28 1404.0 29 1529.0 30 1639.0 31 2007.0 32 2308.0 33 2752.0 34 3416.0 35 3852.0 36 4391.0 37 4229.0 38 2618.0 39 646.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.48312781510744 0.33227497600236283 0.12060350980826504 0.06399369908193654 8 98.62167417361984 1.080508996037313 0.1649068399419134 0.13290999040094514 9 94.69486226336132 3.2175475739149704 1.186578370793432 0.9010117919302822 10 39.15675994978956 44.40178197838982 7.470033719756825 8.971424352063796 11 26.590036428079156 25.64487545535099 26.50881165698533 21.256276459584523 12 41.60082699549583 16.22486401338945 18.531098476457704 23.643210514657017 13 21.792315833517932 19.12673213714342 21.425582711856066 37.65536931748258 14 32.65798478619433 16.07050540360897 22.060018217178307 29.21149159301839 15 23.97770433581611 22.315394860158833 21.777733931830515 31.929166872194543 16 23.08071108484759 20.2910326488403 33.75190820899197 22.876348057320136 17 40.55291597941951 18.90647694542232 18.409197213264076 22.131409861894095 18 39.10003938558488 21.38391098857818 17.693974005513983 21.822075620322963 19 27.7245759582482 30.34637256591418 18.73661406661579 23.19243740922183 20 22.602250621752727 25.62852429144813 21.122356011917955 30.646869074881188 21 25.623984640378083 18.06970905331561 34.66745433958548 21.638851966720818 22 28.3565925816535 19.38762952570824 28.10800167368136 24.147776218956903 23 27.497600236284427 34.56398139260134 20.057102069950037 17.881316301164194 24 27.657584483989268 30.190258189962833 18.942134928253218 23.210022397794678 25 25.406123855469136 34.60667519936989 18.502018312493846 21.485182632667126 26 28.86094674556213 20.249013806706113 30.207100591715975 20.682938856015777 27 38.24014575177881 17.13814412684344 22.382253736120344 22.239456385257405 28 28.717582092495807 28.611576767577162 23.22256187752687 19.44827926240016 29 23.498128816230057 30.217155800669687 25.049241678156392 21.235473704943864 30 20.274761799246622 22.382253736120344 36.4994952852254 20.843489179407637 31 19.893931919092257 24.958066107548103 37.9600394671929 17.18796250616675 32 29.45375772345715 20.68286438716983 30.07655761514413 19.786820274228887 33 21.491455204557226 31.4048975364356 30.179280412320285 16.92436684668689 34 20.17100756474386 23.564053914200528 31.584653672720105 24.68028484833551 35 28.33394902105652 25.708656569388005 31.535525181627875 14.421869227927594 36 28.23059670224041 24.8071377517068 30.855495033643066 16.106770512409728 37 29.06767325389189 23.940986356795698 31.83332099770557 15.158019391606839 38 21.94887470111174 25.291493085512855 39.47050558335593 13.289126630019474 39 18.197766356987547 28.832279106542796 28.642024115437835 24.327930421031823 40 12.181106652796354 16.671620349730023 24.8773963441819 46.269876653291725 41 19.40783758965577 18.152036333854515 26.89052689052689 35.54959918596282 42 17.42863487207323 26.47109619797192 33.747008462658215 22.353260467296636 43 11.38121001012871 18.990093628795176 26.238296400602785 43.39039996047333 44 11.715211957732189 17.132322007473583 24.994431933480165 46.158034101314065 45 17.895696751614334 32.09690935081579 33.689061960861636 16.318331936708237 46 26.295958824833153 24.481000812667766 34.31181815943064 14.911222203068435 47 17.4167815639157 21.518613354343117 39.65186133543431 21.412743746306877 48 17.92768829121718 25.570168957194227 40.94872173784543 15.553421013743165 49 11.714871440215378 17.36359819201225 24.509101686961245 46.412428680811125 50 15.187313488050904 25.70103830123067 43.01921227217797 16.092435938540458 51 25.268591469287955 27.28642347304206 32.559460594235475 14.885524463434512 52 15.869839152027277 36.08578558545203 32.08311713982161 15.961258122699086 53 23.044475690076222 29.243444584227536 33.09652433459138 14.615555391104863 54 15.109544816028786 24.80962121398822 35.73699386351874 24.343840106464253 55 17.289247232290357 26.799319476292627 33.942352738121656 21.969080553295363 56 25.451944656818014 27.11174686166671 32.29832046760549 15.137988013909784 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.0 9 4.0 10 5.5 11 7.0 12 14.5 13 48.5 14 75.0 15 104.5 16 134.0 17 112.0 18 90.0 19 120.0 20 150.0 21 172.0 22 237.0 23 280.0 24 340.5 25 401.0 26 505.5 27 610.0 28 749.5 29 1040.0 30 1191.0 31 1336.0 32 1481.0 33 1489.5 34 1498.0 35 1483.0 36 1468.0 37 1430.0 38 1328.0 39 1264.0 40 1110.0 41 956.0 42 846.0 43 736.0 44 730.0 45 724.0 46 735.5 47 848.0 48 949.0 49 1171.0 50 1393.0 51 1617.5 52 1842.0 53 1791.5 54 1705.0 55 1669.0 56 1797.5 57 1926.0 58 2023.5 59 2121.0 60 2205.0 61 2289.0 62 2292.0 63 2237.5 64 2180.0 65 2120.5 66 2061.0 67 1897.5 68 1734.0 69 1524.5 70 1315.0 71 1159.5 72 850.0 73 696.0 74 587.5 75 479.0 76 386.0 77 293.0 78 231.0 79 129.0 80 89.0 81 66.5 82 44.0 83 32.5 84 21.0 85 13.0 86 5.0 87 3.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.01969036894828817 10 0.0 11 0.0024612961185360212 12 0.0 13 0.0 14 0.01969036894828817 15 0.20428757783848972 16 0.03691944177804032 17 0.01969036894828817 18 0.012306480592680106 19 0.01969036894828817 20 0.04430333013364838 21 0.007383888355608063 22 0.0 23 0.0 24 0.0 25 0.0024612961185360212 26 0.16982943217898544 27 0.02953555342243225 28 0.15998424770484138 29 0.03199684954096827 30 0.02953555342243225 31 0.21905535454970587 32 0.014767776711216126 33 0.19198109724580964 34 0.11321962145265697 35 0.0590711068448645 36 0.13783258263801718 37 0.236284427379458 38 0.15260035934923333 39 0.3864234906101553 40 0.6276305102266854 41 0.826995495828103 42 0.24120701961653007 43 0.36919441778040313 44 0.5414851460779246 45 0.13537128651948116 46 0.05414851460779247 47 0.03199684954096827 48 0.06645499520047257 49 0.349504048832115 50 0.20182628171995376 51 0.34458145659504297 52 0.3839621944916193 53 0.2215166506682419 54 0.12798739816387308 55 0.17721332053459352 56 0.20182628171995376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 40629.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.95584434763346 #Duplication Level Percentage of deduplicated Percentage of total 1 94.12707957506514 80.9077260085161 2 3.8226956447040634 6.571660636491177 3 0.8647596140079603 2.229934283393635 4 0.37224751596369154 1.279873981638731 5 0.23480227930017467 1.0091314085997687 6 0.13458179423302694 0.694085505427158 7 0.10308392749763765 0.6202466218710773 8 0.057268848609798695 0.3938073789657634 9 0.037224751596369156 0.2879716458687145 >10 0.220485067147725 3.539343818454798 >50 0.008590327291469805 0.4307268207438037 >100 0.01718065458293961 2.0354918900292898 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 159 0.39134608284722733 No Hit TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 147 0.3618105294247951 No Hit TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 138 0.3396588643579709 No Hit TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 136 0.3347362721208989 No Hit TGTGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 124 0.30520071869846666 No Hit TGTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 123 0.3027394225799306 No Hit TGTGCGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 63 0.15506165546776932 No Hit TGTGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA 59 0.14521647099362525 No Hit TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 53 0.13044869428240913 No Hit TGTGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 49 0.12060350980826504 No Hit TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 46 0.11321962145265697 No Hit TGTGCGGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 45 0.11075832533412094 No Hit TGTGCGGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 45 0.11075832533412094 No Hit TGTGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 41 0.10091314085997688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0049225922370720425 0.0 0.0 0.0 0.0 11 0.007383888355608063 0.0 0.0 0.0 0.0 12 0.012306480592680104 0.0 0.0 0.0 0.0 13 0.012306480592680104 0.0 0.0 0.0 0.0 14 0.017229072829752147 0.0 0.0 0.0 0.0 15 0.02215166506682419 0.0 0.0 0.0 0.0 16 0.02461296118536021 0.0 0.0 0.0 0.0 17 0.03691944177804032 0.0 0.0 0.0 0.0 18 0.041842034015112356 0.0 0.0 0.0 0.0 19 0.0467646262521844 0.0 0.0 0.0 0.0 20 0.0590711068448645 0.0 0.0 0.0 0.0 21 0.06399369908193654 0.0 0.0 0.0 0.0 22 0.06645499520047257 0.0 0.0 0.0 0.0 23 0.07876147579315268 0.0 0.0 0.0 0.0 24 0.08860666026729676 0.0 0.0 0.0 0.0 25 0.08860666026729676 0.0 0.0 0.0 0.0 26 0.09599054862290482 0.0 0.0 0.0 0.0 27 0.09599054862290482 0.0 0.0 0.0 0.0 28 0.10829702921558493 0.0 0.0 0.0 0.0 29 0.11568091757119299 0.0 0.0 0.0 0.0 30 0.118142213689729 0.0 0.0 0.0 0.0 31 0.12060350980826504 0.0 0.0 0.0 0.0 32 0.12060350980826504 0.0 0.0 0.0 0.0 33 0.12060350980826504 0.0 0.0 0.0 0.0 34 0.12060350980826504 0.0 0.0 0.0 0.0 35 0.12060350980826504 0.0 0.0 0.0 0.0 36 0.12060350980826504 0.0 0.0 0.0 0.0 37 0.12306480592680105 0.0 0.0 0.0 0.0 38 0.12306480592680105 0.0 0.0 0.0 0.0 39 0.12306480592680105 0.0 0.0 0.0 0.0 40 0.12306480592680105 0.0 0.0 0.0 0.0 41 0.12306480592680105 0.0 0.0 0.0 0.0 42 0.12306480592680105 0.0 0.0 0.0 0.0 43 0.13783258263801718 0.0 0.0 0.0 0.0 44 0.1402938787565532 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAACC 15 0.007987461 50.59825 38 TTCGTTT 15 0.007987461 50.59825 38 CCAGATG 15 0.007987461 50.59825 40 TCCGCCC 30 9.4193456E-7 50.59825 38 CCCTCCG 15 0.007987461 50.59825 39 TCCGACC 15 0.007987461 50.59825 38 TACTCAC 15 0.007987461 50.59825 38 CAAACCA 15 0.007987461 50.59825 39 CCGACCA 15 0.007987461 50.59825 39 CGACCAG 15 0.007987461 50.59825 40 GACCAGT 15 0.008027123 50.535 41 AAGAGCA 25 1.9693067E-5 50.346207 35 ACAGAAT 15 0.008268183 50.15881 42 TAGTTCT 15 0.008268183 50.15881 44 TCTTCTC 15 0.008268183 50.15881 43 CCAACAC 40 2.5011104E-9 50.158806 43 ACCAGTT 20 4.07144E-4 50.158806 42 CTCTGAG 15 0.008308877 50.096657 47 GTTCTTA 15 0.008308877 50.096657 46 TCTATTT 15 0.008308877 50.096657 46 >>END_MODULE