FastQCFastQC Report
Fri 17 Jun 2016
SRR1526341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25275
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1120.4431256181998021No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA520.20573689416419388No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAACCAAAAAAAAAAAAAAA440.17408506429277942No Hit
GCTGTGGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTCCTGGCTGTTGCCAAAA430.1701285855588526No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA410.16221562809099901No Hit
GCTGTGGGGTGCTGCTGAGCACCTTTCTGGGATGTTGTCCTGGCTGTTGCCAAAAA390.1543026706231454No Hit
GCTGTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC390.1543026706231454No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA380.15034619188921858No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAACAAAAAAA300.11869436201780414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTAA150.008021180550.48795341
GGACCAA150.008021180550.48795340
CTGTAGC150.008021180550.48795341
CAAAACC203.9913115E-450.28634
TAGCGCC204.0310132E-450.1856344
CAAAAGA150.00821446350.1856344
GCGCCGG252.0326012E-549.98608446
CCAACAC451.2551027E-1049.9860843
CACATTC150.00834522949.9860824
CATTCCA150.00834522949.9860826
ACACATT150.00834522949.9860823
ACATTCC150.00834522949.9860825
GACACAT150.00834522949.9860822
GCCGGAA150.00834522949.9860848
TTCCATC150.00834522949.9860828
CCATCCC150.00834522949.9860830
CTCAGCA150.00834522949.9860825
TGTAGCG150.00834522949.9860842
ATTCCAT150.00834522949.9860827
GTAGCGC150.00834522949.9860843