FastQCFastQC Report
Fri 17 Jun 2016
SRR1526340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31330
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC700.22342802425789976No Hit
TCGGGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT550.17555059048834984No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC540.17235876157037983No Hit
TCGGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG470.15001595914458987No Hit
TCGGGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT440.14044047239067986No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC410.13086498563676985No Hit
TCGGGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT390.12448132780082986No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC350.11171401212894988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACC150.00781797250.84502438
GCACCCA203.7929713E-450.84502439
TTTCATT203.8237037E-450.76221537
TGCTTGC150.00786863450.76221537
ACCCACT150.00786863450.76221541
TTTTCAT251.901661E-550.59740436
TAAGCCC150.00797068750.597436
TCCTTCT204.0444176E-450.19001842
CCAAAAC309.887954E-750.19001443
CTTCTCT150.00828276150.10932544
GGCTCTT507.2759576E-1249.9487239
CGGTTGT252.053127E-549.94872311
CCCCAGA252.053127E-549.94872314
ACAGTGG150.00838877749.9487210
GAAGTTC150.00838877749.948729
GAGCTGT150.00838877749.948729
AAGCAGT150.00838877749.9487210
CCCAGAG204.1420528E-449.94871515
ATGGGCC150.00844216349.868850
ATTGGAG150.00844216349.868829