##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31330
Sequences flagged as poor quality	0
Sequence length	56
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.38953080114906	38.0	38.0	38.0	35.0	40.0
2	36.49725502713054	38.0	36.0	38.0	33.0	40.0
3	36.972071496967764	38.0	37.0	38.0	34.0	39.0
4	36.709032875837856	38.0	36.0	38.0	33.0	39.0
5	36.78799872326843	38.0	36.0	38.0	33.0	39.0
6	34.809256303862114	36.0	33.0	38.0	29.0	39.0
7	37.15103734439834	38.0	38.0	39.0	34.0	40.0
8	35.56878391318225	38.0	35.0	39.0	31.0	40.0
9	35.57969996808171	38.0	35.0	39.0	31.0	40.0
10	35.58892435365465	38.0	35.0	39.0	31.0	40.0
11	32.935812320459625	35.0	30.0	38.0	24.0	39.0
12	31.634471752314077	35.0	28.0	38.0	17.0	39.0
13	31.47654005745292	35.0	28.0	38.0	16.0	39.0
14	31.15952760932014	35.0	27.0	38.0	14.0	39.0
15	31.734280242578997	36.0	28.0	38.0	16.0	39.0
16	32.80769230769231	36.0	30.0	39.0	19.0	40.0
17	32.79103096074051	36.0	30.0	38.0	19.0	40.0
18	32.94819661666135	36.0	30.0	38.0	20.0	40.0
19	33.69533992977976	36.0	31.0	38.0	25.0	40.0
20	34.0152569422279	37.0	32.0	39.0	25.0	40.0
21	35.24391956591127	38.0	35.0	39.0	27.0	40.0
22	34.834216406000635	38.0	34.0	39.0	26.0	40.0
23	35.07069901053303	38.0	35.0	39.0	27.0	40.0
24	34.771113948292374	38.0	33.0	39.0	26.0	40.0
25	34.98333865304819	38.0	34.0	39.0	27.0	40.0
26	35.30293648260453	38.0	35.0	39.0	28.0	40.0
27	35.22914139802106	38.0	35.0	39.0	28.0	40.0
28	35.11359719119055	38.0	35.0	39.0	28.0	40.0
29	35.01452282157676	38.0	35.0	39.0	27.0	40.0
30	34.874816469837214	38.0	35.0	39.0	27.0	40.0
31	34.57140121289499	38.0	34.0	39.0	26.0	40.0
32	33.52323651452282	38.0	33.0	39.0	19.0	40.0
33	34.82872646026173	38.0	35.0	39.0	27.0	40.0
34	34.59782955633578	38.0	35.0	39.0	26.0	40.0
35	34.61481008617938	38.0	35.0	39.0	27.0	40.0
36	32.549856367698695	36.0	31.0	38.0	23.0	39.0
37	32.37159272263007	35.0	31.0	38.0	22.0	39.0
38	31.47111394829237	35.0	30.0	38.0	16.0	39.0
39	29.31276731567188	34.0	27.0	38.0	6.0	39.0
40	25.083306734759017	31.0	11.0	37.0	4.0	39.0
41	21.988764762208746	26.0	9.0	33.0	4.0	37.0
42	25.85649537184807	30.0	21.0	34.0	4.0	37.0
43	21.89348866900734	26.0	8.0	33.0	4.0	37.0
44	22.986370890520266	28.0	10.0	33.0	4.0	37.0
45	25.48292371528886	30.0	20.0	34.0	4.0	37.0
46	25.42007660389403	30.0	16.0	36.0	4.0	38.0
47	24.703702521544844	30.0	10.0	36.0	4.0	38.0
48	24.700670284072775	30.0	11.0	35.0	4.0	38.0
49	22.066198531758697	26.0	5.0	35.0	4.0	38.0
50	24.492084264283434	30.0	10.0	35.0	4.0	38.0
51	25.94165336737951	32.0	4.0	38.0	4.0	39.0
52	25.712543887647623	32.0	4.0	38.0	4.0	39.0
53	25.395786785828278	32.0	4.0	38.0	4.0	39.0
54	24.407085860197892	31.0	4.0	38.0	4.0	39.0
55	24.539738270028728	31.0	4.0	37.0	4.0	39.0
56	23.685828279604213	30.0	4.0	37.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.09989755363115904
1	2	1.437538487717461
1	3	0.566702590116293
1	4	0.24323238256835822
1	5	0.79661828663356
1	6	0.8652662753264551
1	7	0.5486416383311479
1	8	-0.06625204064560108
1	9	0.056356809203080616
1	10	-0.2698689030276853
1	11	-7.268274996736466
1	12	-9.095970867433586
1	13	-22.45805059366815
1	14	-19.27736324024823
1	15	-14.323846903603211
1	16	-21.767246656855477
1	17	-22.197085360044575
1	18	-20.972030178995272
1	19	-7.150092693151159
1	20	-12.033064438989783
1	21	-11.35386599681381
1	22	-5.332727146403492
1	23	-6.0616771799864395
1	24	-6.091949142161603
1	25	-6.097675897977538
1	26	-0.14829392410306497
1	27	-0.3108303588958279
1	28	0.101250867387499
1	29	-0.47358731427507905
1	30	-0.321865516887776
1	31	-1.2468917663647616
1	32	-11.638653717905783
1	33	-1.1213072577042524
1	34	-1.967882488959944
1	35	-0.6682304192037662
1	36	1.8702789008724743
1	37	3.2723315094145313
1	38	4.521366948361546
1	39	6.64410122099946
1	40	10.152391136707983
1	41	-4.099331644560166
1	42	-9.015061592068506
1	43	-13.706343639292363
1	44	-16.340366696424518
1	45	-14.390736359714456
1	46	-18.180448621945143
1	47	-17.959829308644906
1	48	-16.247731663729645
1	49	-14.126716681956161
1	50	-16.1935482445953
1	51	-17.21518341204575
1	52	-16.997876391810973
1	53	-16.984503770358817
1	54	-17.71447047536677
1	55	-15.721404630848568
1	56	-15.156709662492446
2	1	0.38835909209269914
2	2	1.6090128466918188
2	3	0.9176641285778331
2	4	1.0853798184657961
2	5	1.127547773813049
2	6	1.5038880701982507
2	7	1.2497634332029435
2	8	1.784709497815939
2	9	1.8904914245876938
2	10	1.689265712356928
2	11	-4.269877560839031
2	12	-12.030265739228458
2	13	-12.317024952642509
2	14	-15.544190163325155
2	15	-10.355898185654492
2	16	-3.1734966568554768
2	17	-2.1626302318394472
2	18	-7.748472486687579
2	19	-1.5026567957152608
2	20	-2.323929823605166
2	21	3.0587942595964464
2	22	3.1215997766734276
2	23	2.064124102064845
2	24	3.1035636783512146
2	25	2.3125805122788705
2	26	3.0343983835892416
2	27	2.4215414359759677
2	28	2.0050970212336523
2	29	2.5312203780326143
2	30	2.169320380548122
2	31	-0.3742956125186083
2	32	-8.750833205085268
2	33	0.31939787050087887
2	34	0.1707393059118516
2	35	-0.41742913715248164
2	36	2.57140069574427
2	37	3.4301840735170934
2	38	3.6223284868230863
2	39	4.735447374845613
2	40	8.549025752092597
2	41	7.37182220159368
2	42	3.2549704592135456
2	43	-2.419484664933389
2	44	-8.329950029757851
2	45	2.338430306952212
2	46	-9.662019134765655
2	47	-10.252297257362855
2	48	-6.843084227832209
2	49	-10.863094887084365
2	50	-6.675118757415811
2	51	-5.011657771020108
2	52	-4.777523827708407
2	53	-6.981298642153689
2	54	-13.853092270238566
2	55	-5.962590528284466
2	56	-5.064562226595012
3	1	0.589897553631161
3	2	2.1700384877174628
3	3	1.2792025901162916
3	4	1.4094823825683562
3	5	1.3203682866335598
3	6	2.5490162753264585
3	7	1.8348916383311504
3	8	2.7062479593543998
3	9	2.6951068092030823
3	10	1.893881096972315
3	11	0.5142250032635332
3	12	1.1215291325664154
3	13	-5.758050593668152
3	14	-5.601113240248232
3	15	-2.7938469036032103
3	16	3.266503343144521
3	17	2.929164639955424
3	18	2.6792198210047253
3	19	2.1199073068488445
3	20	1.5406855610102141
3	21	2.7023840031861894
3	22	2.518522853596508
3	23	2.122072820013564
3	24	2.7230508578383947
3	25	1.9423241020224609
3	26	1.946706075896934
3	27	1.5666696411041698
3	28	2.3112508673875
3	29	1.7589126857249227
3	30	1.8718844831122254
3	31	2.7118582336352404
3	32	0.6563462820942192
3	33	2.551192742295747
3	34	1.9558675110400543
3	35	2.2405195807962315
3	36	5.136528900872474
3	37	3.9460815094145296
3	38	5.363866948361547
3	39	8.04160122099946
3	40	9.153641136707982
3	41	9.905668355439833
3	42	6.963688407931496
3	43	3.866156360707638
3	44	2.3833833035754814
3	45	6.671763640285544
3	46	2.3508013780548573
3	47	-2.103579308644907
3	48	2.0210183362703553
3	49	-8.77796668195616
3	50	3.0777017554047
3	51	-0.4726834120457504
3	52	-0.0816263918109712
3	53	-2.1295037703588164
3	54	-4.138220475366769
3	55	-0.9651546308485663
3	56	2.1995403375075533
4	1	0.5953520990857015
4	2	1.7409475786265531
4	3	0.8337480446617462
4	4	1.522209655295633
4	5	1.0767319229971974
4	6	1.7453799116900939
4	7	1.7094370928766054
4	8	3.082611595718035
4	9	3.20237953647581
4	10	1.510244733335952
4	11	2.4033159123544436
4	12	2.914256405293685
4	13	3.187403951786397
4	14	1.0334322142972248
4	15	5.744334914578609
4	16	5.844685161326339
4	17	4.890982821773605
4	18	4.30467436645927
4	19	3.9635436704852083
4	20	3.62432192464658
4	21	2.8569294577316455
4	22	3.016704671778328
4	23	2.7638910018317446
4	24	3.464869039656577
4	25	1.8841422838406459
4	26	2.1267060758969336
4	27	2.1903060047405347
4	28	1.687614503751135
4	29	1.9661854129976462
4	30	0.9264299376576801
4	31	1.5082218699988772
4	32	4.1181644639124
4	33	2.609374560477569
4	34	3.1576856928582373
4	35	1.3368832171598726
4	36	4.449256173599746
4	37	4.302445145778169
4	38	6.3565942210888196
4	39	5.7634194028176395
4	40	9.224550227617073
4	41	4.216577446348925
4	42	6.640052044295132
4	43	7.169792724343999
4	44	9.021565121757302
4	45	7.289945458467361
4	46	7.141710468963947
4	47	8.096420691355092
4	48	7.0664728817248985
4	49	2.9783969544074758
4	50	7.401338119041064
4	51	6.310952951590615
4	52	6.240191790007209
4	53	5.181405320550272
4	54	-0.3309477480940437
4	55	5.862118096424158
4	56	6.754085792053008
5	1	1.400917961794427
5	2	2.7806507326154204
5	3	1.7057332023611878
5	4	1.8535640152214228
5	5	1.9464907356131533
5	6	2.709016275326455
5	7	2.454891638331148
5	8	3.7156357144564396
5	9	3.3363312989989993
5	10	3.380003545951908
5	11	5.04932704407986
5	12	7.453774030525601
5	13	8.001133079801239
5	14	7.807458188323199
5	15	7.681255137213114
5	16	6.785687016613913
5	17	6.545899333832978
5	18	6.7633014536577925
5	19	6.028478735420272
5	20	4.632114132438787
5	21	3.9656493093086382
5	22	4.178114690331199
5	23	3.5983993506258045
5	24	4.344275347634316
5	25	3.6778343061040957
5	26	3.3797673003867317
5	27	2.810343110491928
5	28	3.0145161735099464
5	29	3.206259624500433
5	30	2.5167824422958986
5	31	4.349409254043401
5	32	4.532264649441153
5	33	3.0279274361733
5	34	3.2738266947135273
5	35	3.7290910093676644
5	36	5.327549309035742
5	37	5.580367223700247
5	38	6.083866948361546
5	39	8.969356323040273
5	40	9.341804402014105
5	41	3.566484681970447
5	42	9.53021902017639
5	43	12.769421666830084
5	44	12.819709834187726
5	45	10.794212619877381
5	46	11.503862602544654
5	47	12.369890079110199
5	48	11.639385683209127
5	49	12.77060474661527
5	50	11.960150734996539
5	51	10.898336996117518
5	52	11.699189934719637
5	53	10.97212888270241
5	54	10.94177952463323
5	55	11.608314756906537
5	56	10.499540337507554
6	1	0.7194627710224637
6	2	2.2717776181522424
6	3	0.1835504162032464
6	4	0.6060041216987955
6	5	1.0968900257639973
6	6	1.4916249709786271
6	7	1.23750033398332
6	8	3.028856655006571
6	9	2.6664111570291666
6	10	1.4651854447984007
6	11	5.989877177176581
6	12	7.037181306479461
6	13	6.324558101984021
6	14	6.791930238012636
6	15	7.339196574657656
6	16	6.196068560535828
6	17	6.57872985734673
6	18	5.950524168830814
6	19	5.7738203503270995
6	20	4.538946430575436
6	21	2.6719492205774955
6	22	3.3072185057704218
6	23	3.1081597765353024
6	24	3.4787030317514436
6	25	3.2936284498485477
6	26	1.8136625976360676
6	27	1.9962348584954768
6	28	2.3729899978222804
6	29	2.904130077029272
6	30	3.0327540483296147
6	31	1.950119103200457
6	32	2.649389760355085
6	33	1.4520623075131383
6	34	2.593258815387884
6	35	2.039650015578843
6	36	1.6026158573942162
6	37	0.8660815094145313
6	38	2.2143017309702415
6	39	3.016383829695112
6	40	0.6510324410558077
6	41	0.8082770510920092
6	42	6.48629710358367
6	43	10.410504186794594
6	44	10.590339825314611
6	45	6.541328857676849
6	46	9.089931812837467
6	47	10.674681560920313
6	48	8.655800944966007
6	49	9.646381144130796
6	50	7.859440885839483
6	51	9.34731658795425
6	52	9.00619969514555
6	53	8.803539707902054
6	54	9.159170828981054
6	55	8.652236673499257
6	56	7.28214903315973
7	1	1.213533917267526
7	2	2.250038487717461
7	3	1.5155662264799261
7	4	1.0358460189319914
7	5	1.504004650269927
7	6	1.6635617298719083
7	7	1.9776189110584212
7	8	3.109884322990766
7	9	3.116015900112174
7	10	2.6193356424268615
7	11	5.339679548718081
7	12	7.0460745871118675
7	13	7.587403951786396
7	14	7.5607049415699485
7	15	7.062516732760422
7	16	6.167412434053617
7	17	7.072801003591792
7	18	6.263765275550181
7	19	4.395361852303388
7	20	5.333412833737491
7	21	3.3478385486407376
7	22	3.1076137626874143
7	23	3.009345547286287
7	24	3.6921417669293035
7	25	4.175051374749735
7	26	3.544887894078755
7	27	2.7175787320132656
7	28	3.0557963219329523
7	29	2.1343672311794677
7	30	3.1127935740213175
7	31	3.880949142726145
7	32	4.668164463912397
7	33	3.0093745604775677
7	34	3.262231147403689
7	35	2.5959741262507805
7	36	1.8674379917815678
7	37	2.4342633275963514
7	38	2.6747760392706397
7	39	4.49069213009037
7	40	-3.77090431783747
7	41	-9.156149826378348
7	42	4.385506589749678
7	43	8.469792724344
7	44	7.753383303575482
7	45	4.444490913012817
7	46	8.146255923509404
7	47	9.01460250953691
7	48	9.061927427179445
7	49	10.023851499862023
7	50	9.042247209950155
7	51	10.02913476977243
7	52	9.4311008809163
7	53	10.458678047823003
7	54	10.62359770645141
7	55	8.753027187333249
7	56	8.522267610234827
8	1	0.9408066445402525
8	2	2.2273112149901877
8	3	1.2882934992071995
8	4	1.1494823825683582
8	5	1.57218646845174
8	6	2.572652638962822
8	7	2.273073456512968
8	8	3.5189752320816723
8	9	3.7751068092030806
8	10	3.142062915154135
8	11	5.703315912354441
8	12	7.864256405293688
8	13	8.201040315422755
8	14	8.197068577933589
8	15	7.6761530963967886
8	16	6.98559425223543
8	17	6.981891912682698
8	18	6.604674366459275
8	19	5.327180034121568
8	20	5.537958288282944
8	21	4.2796567304589175
8	22	4.403068308141961
8	23	3.554800092740834
8	24	3.91941449420203
8	25	3.675051374749735
8	26	3.2494333486242084
8	27	2.7857605501950786
8	28	2.351250867387499
8	29	3.3616399584521943
8	30	4.044611755839497
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115	13	-2.8839765195940785
115	14	-4.584076203211193
115	15	-5.333106162862471
115	16	-6.61794110129992
115	17	-6.068613137822354
115	18	-1.8111505493656423
115	19	-1.4000926931511586
115	20	-1.6430181426934851
115	21	-0.012430811628625804
115	22	0.791115446189103
115	23	-0.06977903183829426
115	24	1.6904582652458018
115	25	0.7171389168372784
115	26	1.9859653351561946
115	27	2.589632604067134
115	28	0.9253249414615752
115	29	2.0240978709101043
115	30	1.1978104090381478
115	31	1.4718582336352384
115	32	-4.967357421609485
115	33	1.6726742237772285
115	34	1.375867511040056
115	35	0.967186247462898
115	36	3.4431955675391386
115	37	3.606822250155272
115	38	4.824607689102287
115	39	7.470490109888345
115	40	9.933641136707983
115	41	6.303446133217612
115	42	3.3518365560796433
115	43	-2.467917713366436
115	44	-1.5151352149430366
115	45	0.5236154921373952
115	46	-5.001050473796994
115	47	-3.451727456793055
115	48	-3.7234261081740883
115	49	-7.5838926078820865
115	50	-1.6808167631138176
115	51	0.4954647361023987
115	52	-0.11940416958875133
115	53	-1.1095037703588169
115	54	1.0158535987073023
115	55	0.8607712950773596
115	56	1.0921329301001457
116	1	0.33137903511264
116	2	2.101890339569316
116	3	1.1199433308570335
116	4	0.7235564566424344
116	5	0.9366645829298577
116	6	2.0423496086597908
116	7	1.2326694161089264
116	8	1.4684701815766203
116	9	1.7195512536475235
116	10	1.1109181340093528
116	11	-2.7798490708105383
116	12	-0.8992116081743262
116	13	-5.439532075149632
116	14	-9.76926138839638
116	15	-4.0738469036032114
116	16	-7.173496656855477
116	17	-10.105650174859388
116	18	-1.5518912901063828
116	19	-4.5852778783363455
116	20	-2.791166290841634
116	21	1.6171988180010075
116	22	2.791115446189103
116	23	1.522813560754301
116	24	1.6534212282087708
116	25	2.3467685464669046
116	26	1.2822616314524922
116	27	2.8488918633263935
116	28	2.7031027192393537
116	29	2.394468241280478
116	30	2.197810409038148
116	31	0.6200063817833836
116	32	-7.078468532720596
116	33	1.0430445941476023
116	34	1.1166082517807965
116	35	0.5227418030184552
116	36	2.4802326045761767
116	37	2.4586741020071265
116	38	3.7134965779911724
116	39	5.989008628406864
116	40	9.304011507078357
116	41	8.303446133217612
116	42	4.2407254449685325
116	43	-10.875325120773844
116	44	2.6700499702421503
116	45	2.486578455100357
116	46	-4.149198621945143
116	47	-7.599875604941202
116	48	-5.6123149970629775
116	49	-6.0653740893635675
116	50	-3.458594540891596
116	51	-1.2823130416753798
116	52	-1.4897745399591216
116	53	-1.1095037703588169
116	54	-5.132294549440843
116	55	-4.917006482700419
116	56	-1.2041633661961484
117	1	-1.4167691130355067
117	2	0.050038487717458224
117	3	-0.7541307432170399
117	4	-0.9505176174316432
117	5	-0.6929650466997757
117	6	-0.6909837246735435
117	7	-1.0117750283355207
117	8	-0.15375204064559966
117	9	-0.9915598574635851
117	10	-0.45945223636101673
117	11	-10.505774996736466
117	12	-17.625137534100254
117	13	-13.891383927001485
117	14	-10.754446573581564
117	15	-16.007180236936545
117	16	-16.173496656855477
117	17	-15.357502026711241
117	18	-8.374113512328606
117	19	-8.466759359817825
117	20	-5.872647772323116
117	21	-1.7976159968138106
117	22	-4.201477146403491
117	23	-1.3512605133197724
117	24	-4.916949142161602
117	25	-1.431009231310874
117	26	-0.33996059076973495
117	27	0.49333630777083926
117	28	0.05125086738750184
117	29	-0.49442064760841475
117	30	-0.5947821835544431
117	31	-1.194808433031426
117	32	-12.530320384572448
117	33	-1.5421405910375867
117	34	-0.9574658222932797
117	35	-1.7661470858704327
117	36	-0.29013776579419215
117	37	1.199414842747867
117	38	0.4172002816948783
117	39	0.18160122099946108
117	40	1.6003078033746512
117	41	1.3256683554398343
117	42	-1.1963115920685041
117	43	-8.193843639292364
117	44	-5.72995002975785
117	45	-1.1282363597144567
117	46	-4.349198621945142
117	47	-12.570245975311574
117	48	-4.1456483303963125
117	49	-9.991300015289495
117	50	-5.6289649112619635
117	51	-3.186016745379085
117	52	-3.3416263918109728
117	53	-3.109503770358817
117	54	-9.991553808700104
117	55	-4.931821297515235
117	56	-2.3004596624924467
118	1	0.5641832679168743
118	2	1.9643242020031764
118	3	0.9030121139258185
118	4	0.9351966682826429
118	5	0.9737016199668957
118	6	1.661397227707404
118	7	0.7882249716644836
118	8	1.5795812926877346
118	9	1.7036782377745112
118	10	1.4548334779246943
118	11	-8.467679758641228
118	12	-11.920375629338348
118	13	-13.719955355572914
118	14	-2.573494192629184
118	15	-17.026227855984164
118	16	-5.74492522828405
118	17	-14.433692502901717
118	18	-0.8217325599476517
118	19	-2.923902216960684
118	20	-2.558362058037403
118	21	1.2785744793766654
118	22	0.2937609488346027
118	23	0.06778710572784519
118	24	-2.4788539040663657
118	25	0.6642288639272209
118	26	3.2028965520874095
118	27	2.626669641104172
118	28	1.803631819768448
118	29	2.4103412571534903
118	30	2.7480749593026985
118	31	-0.19480843303142592
118	32	-12.835082289334355
118	33	1.2388117899147986
118	34	1.280629415801961
118	35	1.062424342700993
118	36	3.6336717580153284
118	37	3.9137005570335823
118	38	5.7981526626472615
118	39	6.724458363856606
118	40	9.981260184327034
118	41	11.097096926868403
118	42	4.298926503169589
118	43	7.463299217850494
118	44	5.24147854167072
118	45	4.100335068856971
118	46	-0.8158652886118105
118	47	-4.9226269276925265
118	48	-6.612314997062979
118	49	-2.657966681956161
118	50	-0.9242030065000613
118	51	0.8235070641447244
118	52	-1.8178168680014473
118	53	-2.5856942465492914
118	54	-10.724887142033436
118	55	-5.446107011800947
118	56	-5.4528406148733986
119	1	0.849897553631159
119	2	2.107181344860315
119	3	1.331583542497242
119	4	1.5066252397112123
119	5	1.64036828663356
119	6	2.0899686562788347
119	7	1.4072725907120969
119	8	1.29386700697345
119	9	1.2274877615840296
119	10	1.1215001445913657
119	11	-9.134346425307893
119	12	-10.920375629338348
119	13	-2.3390029746205308
119	14	-1.5734941926291839
119	15	-8.883370713127022
119	16	-5.030639513998334
119	17	-8.57654964575886
119	18	-1.440780178995272
119	19	-0.8286641217225856
119	20	-1.6059811056564506
119	21	1.611907812710001
119	22	1.4366180916917486
119	23	1.401120439061181
119	24	-1.7645681897806504
119	25	2.0451812448796076
119	26	1.2981346473255044
119	27	1.769526783961318
119	28	2.08934610548274
119	29	2.3627222095344464
119	30	1.7956940069217495
119	31	-1.5281417663647616
119	32	-8.358891813143877
119	33	-0.33261678151377794
119	34	0.6139627491352968
119	35	0.39575767603432865
119	36	5.10986223420581
119	37	4.580367223700247
119	38	6.369581234075831
119	39	7.248267887666124
119	40	11.933641136707983
119	41	9.430430260201739
119	42	6.72749793174102
119	43	-2.012891258339984
119	44	0.5748118750040554
119	45	-0.756807788285883
119	46	-6.720627193373716
119	47	-7.589293594359193
119	48	-10.707553092301074
119	49	-7.800823824813303
119	50	-4.1622982445952985
119	51	-5.7003024596647975
119	52	-6.436864487049068
119	53	-7.919027579882627
119	54	-8.772506189652484
119	55	-11.684202249896186
119	56	-7.738554900587683
120	1	0.6325062492833311
120	2	1.641342835543547
120	3	1.161811285768465
120	4	1.2581780347422722
120	5	0.9881943735900833
120	6	1.8394510579351504
120	7	1.0635872905050618
120	8	1.1592914376152663
120	9	0.796845939637862
120	10	0.8999680534940566
120	11	-7.401427170649509
120	12	0.36326826300119563
120	13	-3.349354941494237
120	14	-3.9689393272047546
120	15	-4.856455599255387
120	16	-5.738714048159824
120	17	-7.551704925261966
120	18	-3.092954092038749
120	19	-2.747918780107682
120	20	-4.852357917250654
120	21	0.6284709597079328
120	22	1.0246098101182497
120	23	0.08642064610051392
120	24	-3.2386882725963844
120	25	-1.1846324197166709
120	26	1.5962712932882397
120	27	0.017973988930258145
120	28	1.5469030413005456
120	29	1.2736952944205697
120	30	0.66318883093831
120	31	-1.6150982881038907
120	32	-9.437566761384044
120	33	-4.069676822921643
120	34	-11.450219445481682
120	35	-1.6560021583342035
120	36	1.037398466089865
120	37	0.9530380311536639
120	38	1.0838669483615462
120	39	3.929427307955983
120	40	-0.32722842850940737
120	41	2.5691466163094
120	42	-2.578920287720681
120	43	-5.176452334944537
120	44	-6.127051479033213
120	45	-12.19780157710576
120	46	-14.366589926292969
120	47	-15.477492352123168
120	48	-13.40941644633834
120	49	-15.31014059499964
120	50	-12.684037375030082
120	51	-12.739639933784881
120	52	-11.863365522245756
120	53	-13.718199422532729
120	54	-11.05822047536677
120	55	-11.804285065631177
120	56	-9.587416184231577
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	52.0
5	15.0
6	99.0
7	53.0
8	85.0
9	106.0
10	41.0
11	49.0
12	45.0
13	48.0
14	75.0
15	114.0
16	142.0
17	188.0
18	234.0
19	338.0
20	434.0
21	522.0
22	652.0
23	779.0
24	912.0
25	969.0
26	1037.0
27	1057.0
28	1201.0
29	1274.0
30	1414.0
31	1609.0
32	1876.0
33	2310.0
34	2630.0
35	3215.0
36	3348.0
37	2633.0
38	1439.0
39	335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.33290775614427	0.49792531120331945	0.1053303542930099	0.06383657835939993
8	99.06160229811682	0.5234599425470794	0.15001595914458987	0.26492180019150974
9	97.0023942537909	1.7525937749401435	0.699122106943336	0.5458898643256186
10	55.975103734439834	27.934886690073412	7.555059048834982	8.534950526651771
11	35.85495866449616	27.428261355293813	17.000223435155924	19.716556545054104
12	35.515480370252156	13.434407915735717	23.201404404723906	27.848707309288223
13	25.378231726779443	13.105649537184808	25.323970635173954	36.19214810086179
14	22.6060857626361	14.406590248730803	29.209106293304384	33.77821769532871
15	26.322184562256183	15.357805205602098	27.300633113768626	31.019377118373086
16	31.384507312088893	16.919982118909253	24.426847180535155	27.268663388466695
17	28.79581151832461	19.406844592006127	23.480398416549612	28.316945473119652
18	29.013311201200242	23.22916334152648	22.198103872059246	25.559421585214032
19	29.973821989528798	26.478099859532627	20.150683182224494	23.397394968714085
20	29.117844257846176	25.213115800900354	23.351744835733214	22.31729510552026
21	28.70275791624106	21.501532175689476	24.291368743615934	25.504341164453525
22	27.29971273539738	22.039578678582828	26.728375359080754	23.932333226939036
23	30.17235876157038	22.51516118736036	24.532397063517394	22.780082987551868
24	31.12990743696138	19.86913501436323	24.864347270986276	24.136610277689115
25	27.27736993297159	22.55027130545803	24.503670603255664	25.668688158314712
26	27.30674513064893	19.327086065180545	27.399494674896857	25.966674129273677
27	23.6556392898199	18.006769702388556	28.841486779920807	29.496104227870738
28	23.573621593961878	22.834847128054243	29.608545477804782	23.982985800179097
29	26.173296724347107	20.650022348509033	30.34927526977843	22.82740565736543
30	24.497030082391262	18.79031742990356	34.04547486747142	22.667177620233762
31	26.356539544407475	18.85398003583312	33.20962375223957	21.579856667519838
32	30.292390194075587	16.528345250255363	31.617083758937692	21.562180796731358
33	27.951622192359377	18.10968196070903	33.96685224291291	19.971843604018684
34	22.61539445083749	19.4572305331799	39.04871499808208	18.878660017900526
35	21.317705671946857	23.971640265712825	36.730327031170155	17.98032703117016
36	22.63819175804853	26.18050449183158	32.99977620767927	18.181527542440616
37	25.460770510687315	23.176116728529376	33.72584154614104	17.637271214642265
38	25.476586489252817	24.856064483111567	31.886514841351076	17.780834186284544
39	23.69036557623915	17.30479638588959	30.51488257345167	28.489955464419598
40	15.941331279286219	14.11194556775146	25.665960587970986	44.280762564991335
41	15.913334621080418	17.484386066576523	27.232631511171206	39.36964780117185
42	21.62542812329951	20.063378252936843	35.75429723760443	22.556896386159213
43	13.343371564041776	13.067854168001539	28.842826936631	44.74594733132569
44	15.009140181520797	13.562746544369968	26.44238478560662	44.985728488502616
45	23.263544829790632	20.72878376218635	35.68163656704491	20.326034840978107
46	22.002172107583213	22.110777486743753	33.65169616048042	22.235354245192614
47	18.124341412012647	19.264297346489126	34.64252642334834	27.968834818149883
48	22.237476453497653	21.040196673158583	36.831518789310685	19.890808084033075
49	15.015370821058024	14.7431791981555	25.89663122838478	44.34481875240169
50	19.9264	20.7488	37.8368	21.488
51	20.36574429925698	22.42826031258007	36.16769151934409	21.038303868818858
52	19.664722097570355	22.828386435027888	36.07282518110135	21.434066286300403
53	20.340392859427986	21.719239874592105	38.17262780728134	19.767739458698575
54	18.078680770828672	18.56763925729443	33.43772969863539	29.91595027324151
55	21.39110968979853	22.011512631915576	34.48353054045411	22.113847137831787
56	21.552137626706745	22.93991622166086	34.355514341444696	21.152431810187704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.5
13	5.5
14	9.0
15	18.5
16	28.0
17	40.0
18	52.0
19	70.5
20	89.0
21	138.0
22	197.5
23	208.0
24	248.0
25	288.0
26	339.0
27	390.0
28	476.5
29	637.0
30	711.0
31	800.0
32	889.0
33	896.5
34	904.0
35	927.0
36	950.0
37	840.0
38	664.0
39	598.0
40	535.5
41	473.0
42	506.0
43	539.0
44	520.0
45	501.0
46	538.0
47	616.0
48	657.0
49	737.0
50	817.0
51	845.5
52	874.0
53	950.5
54	1100.5
55	1174.0
56	1340.0
57	1506.0
58	1651.0
59	1796.0
60	1989.0
61	2182.0
62	2280.0
63	2430.5
64	2483.0
65	2353.0
66	2223.0
67	2004.0
68	1785.0
69	1483.5
70	1182.0
71	1033.5
72	785.5
73	686.0
74	539.5
75	393.0
76	341.0
77	289.0
78	234.0
79	130.0
80	81.0
81	55.0
82	29.0
83	18.5
84	8.0
85	7.0
86	6.0
87	3.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.015959144589849983
10	0.0
11	0.003191828917969997
12	0.0
13	0.0
14	0.035110118097669965
15	0.17874241940631982
16	0.038301947015639964
17	0.019150973507819982
18	0.009575486753909991
19	0.019150973507819982
20	0.028726460261729973
21	0.006383657835939994
22	0.0
23	0.0
24	0.0
25	0.0
26	0.20108522183210978
27	0.044685604851579956
28	0.1978933929141398
29	0.025534631343759977
30	0.051069262687519955
31	0.23619533992977976
32	0.006383657835939994
33	0.24257899776571976
34	0.14682413022661986
35	0.05745292052345995
36	0.16278327481646984
37	0.24896265560165973
38	0.2106607085860198
39	0.3798276412384296
40	0.5489945738908394
41	0.8554101500159592
42	0.2840727736993297
43	0.3702521544845196
44	0.4755825087775295
45	0.14363230130864987
46	0.07660389403127993
47	0.04149377593360996
48	0.028726460261729973
49	0.3255665496329397
50	0.2553463134375997
51	0.3383338653048197
52	0.4213214171720396
53	0.2298116820938398
54	0.12448132780082986
55	0.1915097350781998
56	0.1819342483242898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	31330.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.05936801787423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.63151980861937	82.45132460900095
2	4.106709195693936	7.232684328120014
3	1.1018884337960781	2.910947973188637
4	0.43858059371488634	1.5448451962974785
5	0.2102287143426728	0.9256303862112991
6	0.17398238428359128	0.9192467283753591
7	0.09424045815361194	0.5809128630705395
8	0.03987096306498967	0.2808809447813597
9	0.05074486208271413	0.40217044366421956
>10	0.14136068723041792	2.1800191509735076
>50	0.010873899017724455	0.5713373763166294
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	70	0.22342802425789976	No Hit
TCGGGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT	55	0.17555059048834984	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	54	0.17235876157037983	No Hit
TCGGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	47	0.15001595914458987	No Hit
TCGGGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT	44	0.14044047239067986	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	41	0.13086498563676985	No Hit
TCGGGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT	39	0.12448132780082986	No Hit
TCGGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	35	0.11171401212894988	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0031918289179699967	0.0	0.0	0.0	0.0
13	0.0031918289179699967	0.0	0.0	0.0	0.0
14	0.0063836578359399935	0.0	0.0	0.0	0.0
15	0.0063836578359399935	0.0	0.0	0.0	0.0
16	0.009575486753909991	0.0	0.0	0.0	0.0
17	0.009575486753909991	0.0	0.0	0.0	0.0
18	0.015959144589849983	0.0	0.0	0.0	0.0
19	0.015959144589849983	0.0	0.0	0.0	0.0
20	0.019150973507819982	0.0	0.0	0.0	0.0
21	0.019150973507819982	0.0	0.0	0.0	0.0
22	0.019150973507819982	0.0	0.0	0.0	0.0
23	0.019150973507819982	0.0	0.0	0.0	0.0
24	0.025534631343759974	0.0	0.0	0.0	0.0
25	0.031918289179699966	0.0	0.0	0.0	0.0
26	0.035110118097669965	0.0	0.0	0.0	0.0
27	0.035110118097669965	0.0	0.0	0.0	0.0
28	0.035110118097669965	0.0	0.0	0.0	0.0
29	0.035110118097669965	0.0	0.0	0.0	0.0
30	0.035110118097669965	0.0	0.0	0.0	0.0
31	0.038301947015639964	0.0	0.0	0.0	0.0
32	0.04149377593360996	0.0	0.0	0.0	0.0
33	0.04149377593360996	0.0	0.0	0.0	0.0
34	0.04149377593360996	0.0	0.0	0.0	0.0
35	0.04149377593360996	0.0	0.0	0.0	0.0
36	0.04149377593360996	0.0	0.0	0.0	0.0
37	0.04149377593360996	0.0	0.0	0.0	0.0
38	0.04149377593360996	0.0	0.0	0.0	0.0
39	0.04149377593360996	0.0	0.0	0.0	0.0
40	0.04149377593360996	0.0	0.0	0.0	0.0
41	0.044685604851579956	0.0	0.0	0.0	0.0
42	0.047877433769549956	0.0	0.0	0.0	0.0
43	0.047877433769549956	0.0	0.0	0.0	0.0
44	0.047877433769549956	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCACC	15	0.007817972	50.845024	38
GCACCCA	20	3.7929713E-4	50.845024	39
TTTCATT	20	3.8237037E-4	50.762215	37
TGCTTGC	15	0.007868634	50.762215	37
ACCCACT	15	0.007868634	50.762215	41
TTTTCAT	25	1.901661E-5	50.597404	36
TAAGCCC	15	0.007970687	50.5974	36
TCCTTCT	20	4.0444176E-4	50.190018	42
CCAAAAC	30	9.887954E-7	50.190014	43
CTTCTCT	15	0.008282761	50.109325	44
GGCTCTT	50	7.2759576E-12	49.948723	9
CGGTTGT	25	2.053127E-5	49.948723	11
CCCCAGA	25	2.053127E-5	49.948723	14
ACAGTGG	15	0.008388777	49.94872	10
GAAGTTC	15	0.008388777	49.94872	9
GAGCTGT	15	0.008388777	49.94872	9
AAGCAGT	15	0.008388777	49.94872	10
CCCAGAG	20	4.1420528E-4	49.948715	15
ATGGGCC	15	0.008442163	49.8688	50
ATTGGAG	15	0.008442163	49.8688	29
>>END_MODULE