FastQCFastQC Report
Fri 17 Jun 2016
SRR1526339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36589
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2150.5876082975757742No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2110.5766760501790156No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1610.44002295771953315No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1520.41542540107682635No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1350.3689633496406024No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG990.27057312306977505No Hit
TGAAGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC810.22137800978436142No Hit
TGAAGCGGGGATGAGATGGACTCAAATGAATTGACGGGTCCGCACAGTTCTGAGTC690.18858126759408567No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG670.18311514389570638No Hit
TGAAGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC630.17218289649894777No Hit
TGAAGCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC490.1339200306102927No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG460.12572084506272377No Hit
TGAAGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTC400.10932247396758588No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG380.10385635026920659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC251.9030818E-550.61977839
ACGCCCA309.3647395E-750.61977440
CGCCCAC203.8859944E-450.61977441
CCTGTCT309.3647395E-750.61977439
CCGCACA451.1459633E-1050.61977440
TCGTCTC150.0079674850.61977440
GGCCAAC150.0079674850.61977438
CTGTCTC309.3647395E-750.61977440
GCCTCAA451.1641532E-1050.47916442
GCCCACT203.9398507E-450.47916442
ACCGACT203.9398507E-450.47916442
TGCTTGC402.3810571E-950.40915737
AGGCCGC203.9670005E-450.40915737
AAACCAC150.008100159550.40915337
TGCCCGT354.7830326E-850.40915337
TGCTCGT150.008100159550.40915337
CCTCAAC354.8357833E-850.33933643
ATTTTAT251.9668898E-550.33933644
CCAAAAC451.200533E-1050.33933343
CTCCACG203.9942987E-450.33933344