##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526339_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36589 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.17043373691546 39.0 38.0 39.0 33.0 40.0 2 36.47079723414141 38.0 36.0 39.0 30.0 40.0 3 35.225122304517754 38.0 35.0 39.0 26.0 40.0 4 34.415507392932305 38.0 35.0 39.0 17.0 40.0 5 36.13118696876111 38.0 35.0 39.0 30.0 40.0 6 36.43250703763426 38.0 36.0 39.0 30.0 40.0 7 36.320314848725026 38.0 36.0 39.0 31.0 40.0 8 36.2853316570554 38.0 36.0 39.0 30.0 40.0 9 36.36844406788926 38.0 36.0 39.0 30.0 40.0 10 35.59116127797972 38.0 35.0 39.0 28.0 40.0 11 34.29585394517478 37.0 32.0 39.0 26.0 40.0 12 32.903495586105116 36.0 31.0 39.0 19.0 40.0 13 32.5834813741835 36.0 30.0 39.0 17.0 40.0 14 32.0310202519883 36.0 29.0 39.0 15.0 40.0 15 32.47495148815218 36.0 30.0 39.0 17.0 40.0 16 32.49856514252917 36.0 30.0 38.0 18.0 40.0 17 32.493454316871194 36.0 30.0 38.0 19.0 40.0 18 32.788351690398756 36.0 30.0 38.0 20.0 40.0 19 33.50482385416382 36.0 31.0 38.0 24.0 40.0 20 33.726803137555 36.0 31.0 38.0 25.0 40.0 21 35.2118122933122 38.0 35.0 39.0 27.0 40.0 22 34.95935937030255 38.0 34.0 39.0 27.0 40.0 23 35.14190057120992 38.0 35.0 39.0 27.0 40.0 24 34.66446199677499 38.0 33.0 39.0 26.0 40.0 25 34.97294268769302 38.0 34.0 39.0 27.0 40.0 26 35.200360764164095 38.0 35.0 39.0 28.0 40.0 27 35.27601191614966 38.0 35.0 39.0 28.0 40.0 28 34.95700893711225 38.0 35.0 39.0 27.0 40.0 29 34.87477110607013 38.0 35.0 39.0 27.0 40.0 30 34.61239716854792 38.0 34.0 39.0 26.0 40.0 31 34.40730820738473 38.0 34.0 39.0 25.0 40.0 32 33.48489983328323 38.0 33.0 39.0 19.0 40.0 33 34.71442236737818 38.0 35.0 39.0 27.0 40.0 34 34.41807647107054 38.0 34.0 39.0 26.0 40.0 35 34.560906283309194 38.0 34.0 39.0 27.0 40.0 36 32.51173850064227 35.0 31.0 38.0 23.0 39.0 37 32.27929159036869 35.0 31.0 38.0 22.0 39.0 38 31.457514553554347 35.0 30.0 38.0 16.0 39.0 39 29.047145316898522 33.0 26.0 38.0 6.0 39.0 40 25.204077728278993 31.0 12.0 37.0 4.0 39.0 41 21.673945721391675 25.0 9.0 33.0 4.0 37.0 42 25.71786602530815 30.0 21.0 34.0 4.0 37.0 43 21.75703080160704 26.0 8.0 33.0 4.0 36.0 44 23.093334062149825 28.0 10.0 33.0 4.0 37.0 45 25.346688895569706 30.0 20.0 34.0 4.0 37.0 46 25.265626281122742 30.0 15.0 36.0 4.0 38.0 47 24.592254502719396 30.0 10.0 36.0 4.0 38.0 48 24.608680204433025 30.0 11.0 35.0 4.0 38.0 49 22.10967777200798 27.0 5.0 35.0 4.0 38.0 50 24.36792478613791 30.0 4.0 35.0 4.0 38.0 51 25.686435814042472 32.0 4.0 38.0 4.0 39.0 52 25.540845609336138 32.0 4.0 38.0 4.0 39.0 53 25.081089945065457 32.0 4.0 38.0 4.0 39.0 54 24.187597365328376 31.0 4.0 37.0 4.0 39.0 55 24.304271775670284 31.0 4.0 37.0 4.0 39.0 56 23.2188909235016 30.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.7347107696095208 1 2 2.5256004861865904 1 3 3.832640408498733 1 4 5.141200848229005 1 5 2.8775291402844942 1 6 2.5302415741142354 1 7 2.851537943794739 1 8 2.944177928755586 1 9 2.797489078473369 1 10 3.048432856557021 1 11 -4.298765301829132 1 12 -7.513704518107186 1 13 -20.59469287829211 1 14 -18.606732207673694 1 15 -10.70176729419007 1 16 -20.272981821634083 1 17 -21.454306015958306 1 18 -19.936914609154975 1 19 -5.406790399624736 1 20 -10.97107853605738 1 21 -5.4727798939122 1 22 -2.230300394886253 1 23 -2.6533609247901637 1 24 -2.725149813554541 1 25 -2.8098449278866013 1 26 3.590194026635153 1 27 3.20587798454428 1 28 3.139655926377806 1 29 2.6576057996310354 1 30 3.126423626776706 1 31 2.15185342814776 1 32 -9.286494119601329 1 33 1.4780533069326651 1 34 2.441710643675883 1 35 2.407732637364745 1 36 5.2755001856226365 1 37 5.840927869839586 1 38 6.617530229178115 1 39 8.836066139758536 1 40 11.467680613572266 1 41 -2.2609940359169975 1 42 -6.31312803504116 1 43 -13.87499798852491 1 44 -16.30556081923747 1 45 -15.817490157362247 1 46 -17.94279401685563 1 47 -17.60186966614757 1 48 -16.24019560966564 1 49 -13.924044752998892 1 50 -16.158087027716974 1 51 -17.683876794699263 1 52 -17.700540564728293 1 53 -17.043274480818063 1 54 -17.385862900268076 1 55 -16.531732466606314 1 56 -15.177694238229725 2 1 1.638600929792588 2 2 2.386012385500088 2 3 4.084356655638317 2 4 5.120605882553946 2 5 2.512540581932093 2 6 2.0863056473407795 2 7 2.281743893451491 2 8 2.996809507702956 2 9 2.701379238656436 2 10 2.722345900035279 2 11 -4.488696651943549 2 12 -10.613246852203293 2 13 -14.459681436644512 2 14 -16.742887814081016 2 15 -8.785291321650025 2 16 -3.7432335378812205 2 17 -2.146525695592171 2 18 -11.436914609154975 2 19 -2.3839071044302287 2 20 -3.2330922660344967 2 21 2.9128036301152562 2 22 3.0168391932144374 2 23 2.199041821205263 2 24 3.1547128866742966 2 25 1.8274548432804494 2 26 2.6966013492896153 2 27 1.9495850783657929 2 28 2.3730655373617893 2 29 3.1232808568392727 2 30 2.9605197366165186 2 31 1.3269106363857404 2 32 -8.706402586420552 2 33 0.7778244739807221 2 34 1.2060127031724477 2 35 1.0621948799276737 2 36 4.3372850826478135 2 37 5.774566313775519 2 38 6.966500480894361 2 39 8.536294972710479 2 40 10.989419744007048 2 41 6.895756536165383 2 42 1.7154760839519767 2 43 -3.6736249908132397 2 44 -11.454302238001771 2 45 -1.157307091000689 2 46 -12.847828341798419 2 47 -11.687682023126976 2 48 -10.946145266416211 2 49 -13.211230107689968 2 50 -11.050535540302786 2 51 -11.226210890809103 2 52 -11.455689306147056 2 53 -11.37737059065788 2 54 -16.174192419718878 2 55 -11.628986471182971 2 56 -10.339021469351005 3 1 2.0680441029428493 3 2 2.7185829423269396 3 3 3.929131636568904 3 4 5.553481549983388 3 5 2.7985817718634394 3 6 2.5828731530616054 3 7 2.7375028560754373 3 8 3.347686700685415 3 9 2.7273136398768756 3 10 3.2852749618201784 3 11 0.8766732946620976 3 12 1.3985761836472044 3 13 -5.735043755485094 3 14 -4.81725852346317 3 15 -3.2105392240146315 3 16 2.4287725643308313 3 17 3.221132580532924 3 18 2.6508046890906414 3 19 1.1633850389717537 3 20 1.362254797275952 3 21 3.483360456964995 3 22 2.752155745464627 3 23 3.1361127594203637 3 24 3.230990537322654 3 25 2.5410322650958577 3 26 2.195457184529893 3 27 2.328685002088143 3 28 2.665971715851491 3 29 2.596202290859104 3 30 2.249230644320562 3 31 1.6079937790249517 3 32 -0.9356169266188736 3 33 -0.3728238860497939 3 34 1.538201871746054 3 35 0.8287852689436903 3 36 3.0737457996577255 3 37 3.4549629575588874 3 38 3.968407422160567 3 39 6.581680174846255 3 40 9.93259289427402 3 41 10.160058595661951 3 42 7.441257929871121 3 43 3.3969318360364937 3 44 3.5804040930432315 3 45 5.52461510579565 3 46 -1.2059519115924715 3 47 -5.724676683691431 3 48 -1.4156342061568665 3 49 -9.59071141966556 3 50 -1.5440519399976758 3 51 -3.3856311806641735 3 52 -3.4286107401668886 3 53 -4.00818676151982 3 54 -4.596389216057553 3 55 -4.189627203448417 3 56 0.11177944598080103 4 1 2.2940194891696137 4 2 2.890879355000415 4 3 3.996426739999123 4 4 5.884720094104885 4 5 2.769516571549225 4 6 2.5671622339728373 4 7 2.869998273724036 4 8 3.1759139853148994 4 9 3.2224694398245077 4 10 2.9663433043182152 4 11 3.814353315609992 4 12 3.1060912399446643 4 13 4.4092348514800825 4 14 3.175671562946885 4 15 6.327297906124148 4 16 5.834637974123968 4 17 5.761326348535015 4 18 5.085473450546516 4 19 4.680012428340703 4 20 4.56152162105181 4 21 2.8394746229770362 4 22 3.399707460573289 4 23 2.843104118414864 4 24 3.802344294850805 4 25 3.0393302488612406 4 26 3.4091256841371163 4 27 2.851596759092594 4 28 2.936985070132721 4 29 3.434117975593331 4 30 3.920610586713863 4 31 3.350596554620658 4 32 4.267315778277698 4 33 3.7651703532798777 4 34 3.8812236051841253 4 35 3.491786054489644 4 36 5.0300170748606625 4 37 5.283975788142808 4 38 4.607318131770413 4 39 8.519098347142666 4 40 10.445292553870775 4 41 6.310097872959673 4 42 8.864405350661904 4 43 6.962001225929136 4 44 11.283205873614062 4 45 9.894607250336108 4 46 9.746915331141228 4 47 9.522246594653687 4 48 8.821862520734989 4 49 5.3556096067811545 4 50 7.404363875660874 4 51 6.39546334669901 4 52 6.942428798979485 4 53 6.968508708498511 4 54 0.18758564647190568 4 55 4.388534619018195 4 56 3.8368383619273843 5 1 2.0339310425140056 5 2 2.73222816649848 5 3 3.8540829036254394 5 4 5.124631647449277 5 5 2.0705115964248435 5 6 1.8479803655372393 5 7 2.6029999320988324 5 8 2.441253952147399 5 9 2.5781908328593346 5 10 3.103988412112578 5 11 3.618388694272234 5 12 5.8907789126920385 5 13 4.227919207477871 5 14 4.939076759187902 5 15 5.0263028812485295 5 16 5.666589328463385 5 17 5.440430826146958 5 18 4.322344650104284 5 19 3.595158918114059 5 20 4.489935109166794 5 21 2.9433994433197697 5 22 3.1907522366926955 5 23 2.0766585683872236 5 24 2.7183199720205096 5 25 2.961110237805407 5 26 2.938147243009425 5 27 1.9729345147587054 5 28 2.352131559906077 5 29 3.11764478598581 5 30 2.528957739837132 5 31 2.7463953384791395 5 32 2.9610692332251816 5 33 2.0998856656071254 5 34 3.0957067450404026 5 35 1.9671868283978853 5 36 3.5659485287025596 5 37 3.4686081817304277 5 38 5.04540547284283 5 39 6.788307855158145 5 40 5.171384317275969 5 41 -0.47541898718405307 5 42 7.3096789825026995 5 43 10.819933785354234 5 44 9.126213059904831 5 45 7.88134025199448 5 46 7.58936972583443 5 47 8.196375947887518 5 48 7.964482752907461 5 49 7.827417235305202 5 50 8.636259950840532 5 51 7.781035486002491 5 52 7.735131949891592 5 53 7.8690062209363205 5 54 8.734020140667596 5 55 8.950723673744566 5 56 8.417822330971056 6 1 2.7184428270257897 6 2 3.4366052708755817 6 3 3.9163724659149963 6 4 6.07277979559742 6 5 3.431117657030903 6 6 3.223064540621408 6 7 3.625222154321051 6 8 3.196809507702959 6 9 3.381221135889632 6 10 3.730251038375201 6 11 3.731856707740249 6 12 6.648017969931093 6 13 5.931622911181574 6 14 6.253554873666015 6 15 6.283878638824287 6 16 6.329888991763045 6 17 5.740430826146955 6 18 5.50853993629957 6 19 4.488424911380051 6 20 4.9064334256651065 6 21 3.0693253692456963 6 22 4.1907522366926955 6 23 3.5275003192289773 6 24 4.0408788945794285 6 25 3.7796287563239233 6 26 3.1543088591710386 6 27 3.4594665012906916 6 28 3.34539755317207 6 29 2.428419196760224 6 30 3.4565671674465577 6 31 4.237977830061631 6 32 4.960395832551782 6 33 3.0699193356407974 6 34 4.4364474857811445 6 35 2.925435986647045 6 36 4.589854252608287 6 37 3.391167104289348 6 38 5.267290994728349 6 39 4.607836474686767 6 40 4.4585897044813585 6 41 3.574412662647596 6 42 7.567254740078457 6 43 12.419260384680832 6 44 9.689176022867795 6 45 7.41265338330761 6 46 9.587349523814229 6 47 10.647891099402667 6 48 9.730139318564028 6 49 9.258730366618334 6 50 8.683061297641878 6 51 8.893156698123704 6 52 8.848263263022904 6 53 7.990218342148442 6 54 8.238060544707999 6 55 6.284057007077898 6 56 5.996946910095634 7 1 2.7171669099603974 7 2 3.209811012502378 7 3 4.894043917270665 7 4 6.413130672790402 7 5 3.4477045788809875 7 6 3.1793643811317764 7 7 3.667327417478951 7 8 3.9090902094573394 7 9 3.2360855697014372 7 10 4.144924084627199 7 11 4.911761013960344 7 12 5.3459446046998345 7 13 7.0368860690763135 7 14 7.428355511624549 7 15 7.298232705809934 7 16 7.235790108190482 7 17 6.221132580532924 7 18 6.03676960137134 7 19 5.093209600375268 7 20 4.5026056744689384 7 21 3.185114842929906 7 22 3.9451382016049763 7 23 2.9431303032800145 7 24 4.160815098726161 7 25 2.9445410370256795 7 26 3.0726501669860298 7 27 3.0128955284039307 7 28 3.385269961465525 7 29 3.1225180803327888 7 30 3.6176516969521444 7 31 2.9413271123582803 7 32 4.029295354082883 7 33 3.0131410262309117 7 34 2.941710643675883 7 35 3.1445747426279027 7 36 2.091289659306849 7 37 2.4725068172080107 7 38 2.8280565449675876 7 39 3.9851889467760806 7 40 -1.3481088601119424 7 41 -7.945204562232787 7 42 5.441257929871121 7 43 9.730265169369826 7 44 8.755842689534461 7 45 6.121106333865825 7 46 8.337907737530337 7 47 9.222691737361199 7 48 8.829979828930853 7 49 11.356657001387074 7 50 9.122614726668992 7 51 10.719631977230563 7 52 9.624020838780478 7 53 9.833918501638077 7 54 10.175540608503852 7 55 10.564758761463864 7 56 9.971428568787818 8 1 2.219116227699189 8 2 2.787783722054037 8 3 4.261490311032844 8 4 5.309816832634461 8 5 3.292733818647065 8 6 2.8109433285002012 8 7 2.991888820987718 8 8 3.4227354336288798 8 9 3.5596723143408155 8 10 3.511395819519983 8 11 4.099870175753715 8 12 6.4278159497290694 8 13 6.8390303185889785 8 14 6.494632314743459 8 15 6.618895473841118 8 16 6.944367106241163 8 17 6.199690085406218 8 18 5.618640946400582 8 19 5.539603362558502 8 20 4.61956473879642 8 21 3.2211772210975482 8 22 3.487048532988993 8 23 3.3729548646835212 8 24 3.7368384905390286 8 25 3.424073200768369 8 26 2.3455546504168296 8 27 2.9173789592031483 8 28 2.2410204487949628 8 29 2.988015156356184 8 30 2.6030318139112083 8 31 2.968617560701361 8 32 4.812921085077029 8 33 3.7295152952367587 8 34 3.4845956339292883 8 35 3.4486683098793662 8 36 1.4918744546284906 8 37 2.153793366915611 8 38 3.6565165839539375 8 39 4.825344892195183 8 40 1.6528657987574533 8 41 -9.14208565385072 8 42 6.05041972324344 8 43 9.931044896465345 8 44 9.033620467312236 8 45 7.64059951125374 8 46 9.996777133241839 8 47 9.974153725665293 8 48 10.779297567722274 8 49 11.808898716786683 8 50 10.099222913803494 8 51 11.799554004521013 8 52 12.03142824618789 8 53 12.276413628343729 8 54 12.808094214741672 8 55 11.024797747818639 8 56 12.47337788652661 9 1 2.5339310425140056 9 2 3.1211170553873657 9 3 4.3911199406624775 9 4 5.7912983141159415 9 5 3.1816227075359578 9 6 3.0146470322039036 9 7 3.436333265432168 9 8 3.2745872854807345 9 9 3.355968610637113 9 10 3.7706550787792423 9 11 4.599870175753715 9 12 6.316704838617962 9 13 6.450141429700093 9 14 6.939076759187902 9 15 7.396673251618896 9 16 7.018441180315239 9 17 6.718208603924737 9 18 6.65567798343762 9 19 5.650714473669609 9 20 4.638083257314939 9 21 3.610066109986434 9 22 4.487048532988993 9 23 4.1507326424613 9 24 3.8479496016501358 9 25 3.387036163731331 9 26 3.401110205972387 9 27 3.343304885129079 9 28 3.926205633980146 9 29 3.7472744156154434 9 30 3.0845132953926893 9 31 4.079728671812475 9 32 5.868476640632586 9 33 4.1184041841256445 9 34 4.003114152447807 9 35 4.226446087657145 9 36 3.769652232406262 9 37 4.005645218767466 9 38 4.841701769139128 9 39 6.1216411884914805 9 40 -0.754541608649955 9 41 -2.7531967649618316 9 42 7.5319012047249245 9 43 11.727341192761639 9 44 10.478064911756682 9 45 8.918377289031515 9 46 12.274554911019617 9 47 12.733412984924552 9 48 12.61263090105561 9 49 14.994083901971866 9 50 12.54366735824794 9 51 11.651405856372861 9 52 12.198094912854554 9 53 12.980117332047431 9 54 12.993279399926855 9 55 13.172945895966784 9 56 12.528933442082167 10 1 2.2782330368159975 10 2 3.242199676469987 10 3 4.1838549833975165 10 4 5.618933641751269 10 5 2.903844929758179 10 6 2.7276099951668655 10 7 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116 8 2.8677772496384364 116 9 2.551309112429223 116 10 1.7097231791376686 116 11 -2.0118383069225096 116 12 -2.654621326256592 116 13 -5.8748287017216505 116 14 -10.216239847741608 116 15 -6.38767561337513 116 16 -10.04547757715191 116 17 -10.704730464175622 116 18 -4.017559770445299 116 19 -5.210695323224055 116 20 -4.7639478060064455 116 21 0.8370673047295654 116 22 1.2230103012088236 116 23 1.292309703393201 116 24 1.7493832934064102 116 25 1.1409190789045738 116 26 0.8915522609305668 116 27 0.7321937740179649 116 28 1.4656321572776392 116 29 2.0812051205138857 116 30 0.8055407744846832 116 31 0.1705291497097292 116 32 -7.979193610263469 116 33 -0.7740689341539237 116 34 -1.679681546476921 116 35 -1.4903997904790565 116 36 0.7839891499689884 116 37 0.8222521482776202 116 38 1.4426575636433085 116 39 4.023672251812865 116 40 6.83864835550775 116 41 3.1040314309760433 116 42 -0.3301059637338568 116 43 -11.378393574263448 116 44 -1.7817917190676908 116 45 -0.6706310741703945 116 46 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1.5373167450912604 117 29 2.154681823022848 117 30 0.8252540361334297 117 31 -2.2165676244838224 117 32 -14.909301137145189 117 33 -2.437151371429909 117 34 -4.330219180885525 117 35 -3.49577613456508 117 36 -2.841458878704845 117 37 -2.0128732997510532 117 38 -2.2508908234534672 117 39 2.306826373676664 117 40 0.023236169127823558 117 41 3.043099531334466 117 42 -1.245876573052854 117 43 -9.88376991834947 117 44 -5.3552684215766515 117 45 -4.396437525783298 117 46 -5.8550747186100125 117 47 -8.748068496556929 117 48 -6.868850580425873 117 49 -4.672582764694798 117 50 -4.678554863974284 117 51 -6.774520069553063 117 52 -6.375979161219519 117 53 -7.130993779063678 117 54 -10.099313192665738 117 55 -8.771498548477657 117 56 -3.78588137273265 118 1 1.5220791906621542 118 2 2.2729689072392176 118 3 3.1600088295513657 118 4 3.0327797955974205 118 5 2.383844929758176 118 6 1.4776099951668655 118 7 2.065222154321056 118 8 2.4368095077029537 118 9 2.3448574995260003 118 10 1.548432856557021 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120 14 -4.7646269445158005 120 15 -7.534303179357533 120 16 -14.715565553691498 120 17 -9.923205537489405 120 18 -4.255096427336792 120 19 -2.8661205431654047 120 20 -6.739021119789438 120 21 -1.2215837216634 120 22 -1.8092477633073045 120 23 -2.990681498952842 120 24 -8.222757469056933 120 25 -2.974916698221527 120 26 0.37249067735285735 120 27 0.6412846831088714 120 28 -0.6546024468279299 120 29 -1.6533989850579616 120 30 -3.0434328325534388 120 31 -6.852931260847459 120 32 -12.666876894720946 120 33 -7.502807937086473 120 34 -15.02718887785522 120 35 -6.465473104262049 120 36 -5.528327565573534 120 37 -4.9270147138924685 120 38 -4.750890823453467 120 39 -2.1830726162223257 120 40 -7.1232284773368235 120 41 -2.7346782464433126 120 42 -5.478199805376086 120 43 -7.489830524410078 120 44 -4.68355124985948 120 45 -7.805528434874207 120 46 -12.703559567094862 120 47 -11.97029071877915 120 48 -9.333497045072336 120 49 -14.768542360654395 120 50 -9.198756884176303 120 51 -10.143206938239933 120 52 -9.542645827886187 120 53 -11.964327112397012 120 54 -10.634666728019274 120 55 -10.579579356558465 120 56 -7.184871271722548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 366.0 5 12.0 6 17.0 7 28.0 8 55.0 9 77.0 10 37.0 11 42.0 12 47.0 13 64.0 14 85.0 15 139.0 16 173.0 17 273.0 18 338.0 19 408.0 20 483.0 21 637.0 22 769.0 23 889.0 24 1033.0 25 1090.0 26 1238.0 27 1222.0 28 1375.0 29 1457.0 30 1546.0 31 1794.0 32 2183.0 33 2572.0 34 3037.0 35 3538.0 36 3899.0 37 3464.0 38 1786.0 39 416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.3082347153516 1.6070403673235125 0.046462051436224006 0.03826286588865506 8 99.43425619721774 0.46188745251305036 0.06012736068217223 0.043728989587034355 9 98.21770269531463 0.9977584604450276 0.44557432617134113 0.3389645180689957 10 49.45202109923748 27.910027603924675 13.512257782393617 9.125693514444231 11 29.198097736962943 37.55602929922379 16.05444408002624 17.191428883787033 12 30.63762332941595 12.249583208068 23.67651479952991 33.43627866298614 13 28.10954111891552 13.760966410669873 20.626417775834266 37.50307469458034 14 21.34383029905418 17.762834180744626 28.713574982231698 32.17976053796949 15 32.8606624692034 16.266082671776623 23.36435806186696 27.508896797153028 16 30.73410799726589 20.082023239917977 23.784005468215994 25.399863294600138 17 25.319154752467128 18.65175910992045 31.2757989120035 24.753287225608922 18 31.40272235281255 20.548843820040453 25.845952003498606 22.20248182364839 19 36.24483993548211 22.745291013969766 16.96875256294595 24.041116487602178 20 24.921394395078604 32.40191387559809 18.44702665755297 24.229665071770334 21 23.918331647853066 18.941153961789706 28.999371361412525 28.14114302894471 22 24.452704364699773 19.175161933914563 26.256525185164943 30.11560851622072 23 25.297220476099376 21.15389871272787 23.154499986334688 30.39438082483807 24 22.870261554018967 26.31391948399792 27.188499275738607 23.6273196862445 25 30.990188307961407 25.854765093334066 21.107436661291644 22.047609937412886 26 21.939669330997482 24.504543961458445 24.181539472243514 29.37424723530056 27 25.98403673737153 16.03433194839274 27.05827684233545 30.923354471900282 28 24.924719408705172 25.910210785655625 26.60552970161511 22.55954010402409 29 27.372881355932204 23.794423182066705 27.06670311645708 21.765992345544014 30 20.195724673336613 23.189000054671695 37.67973320212126 18.93554206987043 31 19.249520679265956 24.108463434675432 37.515749109832925 19.12626677622569 32 29.32349323493235 15.533688670220036 34.95694956949569 20.18586852535192 33 26.111841384598534 17.40333004710264 32.65417899003177 23.83064957826706 34 19.181529707845495 28.449502133712663 34.52511215669111 17.843856001750737 35 21.576241522642746 17.364909210238462 41.62108947713848 19.437759789980312 36 24.976055605724763 25.10467120926032 33.06241961524779 16.856853569767125 37 24.39251568364244 19.020354491411666 39.64331698764485 16.943812837301046 38 31.569437980782393 19.776068329272633 33.85529305483315 14.799200635111829 39 18.770057876402337 17.28886084976822 33.15138382203692 30.789697451792524 40 12.479393339927466 15.2901417738213 27.626662270579182 44.60380261567205 41 14.882067673316431 15.339468753444285 30.913148903339575 38.8653146698997 42 27.07574304889741 18.822079167237366 34.81988768661827 19.28229009724695 43 10.757376329933093 12.276516397937918 31.350773280684436 45.61533399144456 44 11.8319143092557 15.273276572370229 26.149409502883824 46.74539961549025 45 18.009413825187455 18.8577527228942 41.62060095232883 21.512232499589516 46 18.454383805225003 22.05990972507181 35.00752291068253 24.478183559020653 47 20.10774447604463 22.13410632246773 34.47549770290965 23.28265149857799 48 18.741966361274443 23.399425680295362 40.445781485026664 17.412826473403527 49 12.350586687136747 15.84055269218116 28.481741419015243 43.32711920166685 50 22.51931401019122 18.122294668785273 38.428031340748454 20.930359980275053 51 19.964349376114082 25.05416152474976 38.54929384341149 16.432195255724668 52 21.681986555083004 21.28412676636027 38.526546851419944 18.507339827136782 53 18.726714880561033 24.449375410913873 39.99561691869384 16.828292789831252 54 19.923916910698157 23.076712553709736 31.309012288239963 25.690358247352147 55 18.162404862289875 24.661884684882 39.00783003887642 18.167880413951707 56 25.319749130446688 19.22055158436721 32.35286062498288 23.106838660203216 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 7.5 13 17.0 14 20.0 15 33.0 16 46.0 17 59.0 18 72.0 19 117.5 20 163.0 21 193.5 22 302.0 23 380.0 24 434.5 25 489.0 26 628.5 27 768.0 28 888.0 29 1134.5 30 1261.0 31 1282.5 32 1304.0 33 1271.5 34 1239.0 35 1097.0 36 955.0 37 854.5 38 679.5 39 605.0 40 573.5 41 542.0 42 549.0 43 556.0 44 557.0 45 558.0 46 770.0 47 1400.5 48 1819.0 49 1848.5 50 1878.0 51 1812.0 52 1746.0 53 1678.0 54 1579.0 55 1548.0 56 1597.0 57 1646.0 58 1710.5 59 1775.0 60 1885.0 61 1995.0 62 2010.5 63 1970.5 64 1915.0 65 1871.0 66 1827.0 67 1581.5 68 1336.0 69 1233.0 70 1130.0 71 1002.5 72 766.0 73 657.0 74 526.0 75 395.0 76 309.0 77 223.0 78 174.0 79 96.0 80 67.0 81 53.0 82 39.0 83 24.0 84 9.0 85 7.5 86 6.0 87 3.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.01913143294432753 10 0.0 11 0.002733061849189647 12 0.0 13 0.0 14 0.01913143294432753 15 0.1612506491021892 16 0.03826286588865506 17 0.021864494793517177 18 0.008199185547568941 19 0.02733061849189647 20 0.03826286588865506 21 0.005466123698379294 22 0.0 23 0.0 24 0.0 25 0.0 26 0.15578452540380988 27 0.013665309245948234 28 0.1612506491021892 29 0.02459755664270682 30 0.01913143294432753 31 0.2159118860859821 32 0.010932247396758589 33 0.19951351499084424 34 0.09019104102325835 35 0.05739429883298259 36 0.12572084506272377 37 0.23504331903030964 38 0.16398371095137884 39 0.3607641640930334 40 0.5274809368936019 41 0.8117193692093252 42 0.22957719533193036 43 0.3307004837519473 44 0.48921807100494685 45 0.1284539069119134 46 0.09292410287244801 47 0.05739429883298259 48 0.06559348438055153 49 0.3088359889584301 50 0.2377763808794993 51 0.33889966929951626 52 0.39356090628330914 53 0.23231025718112 54 0.13665309245948234 55 0.17218289649894777 56 0.20771270053841318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 36589.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.83697286069584 #Duplication Level Percentage of deduplicated Percentage of total 1 93.80496762346574 79.58129492470415 2 3.9882735736606425 6.767061138593567 3 1.050223897425985 2.6729344885074746 4 0.36403466383170646 1.2353439558337205 5 0.19973583325279468 0.8472491732487906 6 0.12564028220740311 0.6395364727103775 7 0.0934248252311459 0.5548115553854984 8 0.06443091395251442 0.4372898958703435 9 0.07087400534776586 0.5411462461395501 >10 0.2061789246480461 3.2988056519719042 >50 0.016107728488128605 1.0358304408428765 >100 0.016107728488128605 2.3886960561917516 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 215 0.5876082975757742 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 211 0.5766760501790156 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 161 0.44002295771953315 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 152 0.41542540107682635 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 135 0.3689633496406024 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 99 0.27057312306977505 No Hit TGAAGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC 81 0.22137800978436142 No Hit TGAAGCGGGGATGAGATGGACTCAAATGAATTGACGGGTCCGCACAGTTCTGAGTC 69 0.18858126759408567 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG 67 0.18311514389570638 No Hit TGAAGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC 63 0.17218289649894777 No Hit TGAAGCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 49 0.1339200306102927 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 46 0.12572084506272377 No Hit TGAAGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTC 40 0.10932247396758588 No Hit TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG 38 0.10385635026920659 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.002733061849189647 0.0 0.0 0.0 0.0 13 0.005466123698379294 0.0 0.0 0.0 0.0 14 0.008199185547568941 0.0 0.0 0.0 0.0 15 0.008199185547568941 0.0 0.0 0.0 0.0 16 0.010932247396758589 0.0 0.0 0.0 0.0 17 0.01913143294432753 0.0 0.0 0.0 0.0 18 0.01913143294432753 0.0 0.0 0.0 0.0 19 0.021864494793517177 0.0 0.0 0.0 0.0 20 0.03006368034108612 0.0 0.0 0.0 0.0 21 0.032796742190275764 0.0 0.0 0.0 0.0 22 0.035529804039465415 0.0 0.0 0.0 0.0 23 0.03826286588865506 0.0 0.0 0.0 0.0 24 0.03826286588865506 0.0 0.0 0.0 0.0 25 0.03826286588865506 0.0 0.0 0.0 0.0 26 0.046462051436224 0.0 0.0 0.0 0.0 27 0.04919511328541365 0.0 0.0 0.0 0.0 28 0.05739429883298259 0.0 0.0 0.0 0.0 29 0.05739429883298259 0.0 0.0 0.0 0.0 30 0.05739429883298259 0.0 0.0 0.0 0.0 31 0.06012736068217224 0.0 0.0 0.0 0.0 32 0.06012736068217224 0.0 0.0 0.0 0.0 33 0.06012736068217224 0.0 0.0 0.0 0.0 34 0.06012736068217224 0.0 0.0 0.0 0.0 35 0.06012736068217224 0.0 0.0 0.0 0.0 36 0.06012736068217224 0.0 0.0 0.0 0.0 37 0.06012736068217224 0.0 0.0 0.0 0.0 38 0.06012736068217224 0.0 0.0 0.0 0.0 39 0.06286042253136188 0.0 0.0 0.0 0.0 40 0.06286042253136188 0.0 0.0 0.0 0.0 41 0.06286042253136188 0.0 0.0 0.0 0.0 42 0.06286042253136188 0.0 0.0 0.0 0.0 43 0.06286042253136188 0.0 0.0 0.0 0.0 44 0.06286042253136188 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCCC 25 1.9030818E-5 50.619778 39 ACGCCCA 30 9.3647395E-7 50.619774 40 CGCCCAC 20 3.8859944E-4 50.619774 41 CCTGTCT 30 9.3647395E-7 50.619774 39 CCGCACA 45 1.1459633E-10 50.619774 40 TCGTCTC 15 0.00796748 50.619774 40 GGCCAAC 15 0.00796748 50.619774 38 CTGTCTC 30 9.3647395E-7 50.619774 40 GCCTCAA 45 1.1641532E-10 50.479164 42 GCCCACT 20 3.9398507E-4 50.479164 42 ACCGACT 20 3.9398507E-4 50.479164 42 TGCTTGC 40 2.3810571E-9 50.409157 37 AGGCCGC 20 3.9670005E-4 50.409157 37 AAACCAC 15 0.0081001595 50.409153 37 TGCCCGT 35 4.7830326E-8 50.409153 37 TGCTCGT 15 0.0081001595 50.409153 37 CCTCAAC 35 4.8357833E-8 50.339336 43 ATTTTAT 25 1.9668898E-5 50.339336 44 CCAAAAC 45 1.200533E-10 50.339333 43 CTCCACG 20 3.9942987E-4 50.339333 44 >>END_MODULE