FastQCFastQC Report
Fri 17 Jun 2016
SRR1526338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81551
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1250.15327831663621536No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1240.15205209010312565No Hit
AAAGTTGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG1180.14469473090458732No Hit
AAAGTTGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1100.13488491863986954No Hit
AAAGTTGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG1040.1275275594413312No Hit
AAAGTTGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1020.12507510637515176No Hit
AAAGTTGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG970.11894397370970312No Hit
AAAGTTGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG880.10790793491189564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCA800.050.41873639
CTGACCA150.00813030350.41873639
CCCACGT150.00813030350.41873639
TACGCCC800.050.41873638
GCCAACC150.00813030350.41873639
CTGAGAT150.008170282550.3562641
TGGCCTT150.008170282550.3562637
AAACCGC150.008170282550.3562637
GTACGCC900.050.3562637
GAGTCCG150.00823052750.26283336
ATACCCA150.00823052750.26283336
CCGAAAC204.1167377E-450.10789543
CCAATAC150.00833166850.1078943
CCAACAC900.050.1078943
CATGACT150.00835200750.0770244
ATTATAT204.1292905E-450.0770244
TCTGATA150.00837238250.04618548
GCCCAAA150.00837238250.04618548
AATCAAT150.00837238250.04618549
CAAAAAG150.00837238250.04618549