##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526338_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81551 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.22099054579343 39.0 38.0 40.0 33.0 40.0 2 37.00393618717122 39.0 38.0 40.0 33.0 40.0 3 36.77974519012643 39.0 38.0 39.0 33.0 40.0 4 36.116356635724884 39.0 36.0 39.0 30.0 40.0 5 35.76162156196736 39.0 35.0 39.0 28.0 40.0 6 36.61127392674523 39.0 38.0 40.0 32.0 40.0 7 36.59437652511925 39.0 38.0 40.0 32.0 40.0 8 36.30572279922993 38.0 36.0 40.0 31.0 40.0 9 36.29287194516315 38.0 36.0 39.0 31.0 40.0 10 36.13267771088031 38.0 36.0 39.0 30.0 40.0 11 35.193277826145604 38.0 34.0 39.0 28.0 40.0 12 33.00237887947419 38.0 31.0 39.0 17.0 40.0 13 32.359529619501906 37.0 30.0 39.0 15.0 40.0 14 32.09801228678986 37.0 29.0 39.0 14.0 40.0 15 32.619550955843586 37.0 30.0 39.0 17.0 40.0 16 32.216968522764894 36.0 29.0 39.0 16.0 40.0 17 32.35496805679881 36.0 30.0 39.0 17.0 40.0 18 32.47604566467609 36.0 30.0 39.0 18.0 40.0 19 33.45443955316305 36.0 31.0 39.0 23.0 40.0 20 33.53230493801425 37.0 31.0 39.0 23.0 40.0 21 34.901276501820945 38.0 35.0 39.0 26.0 40.0 22 34.66147564100992 38.0 34.0 39.0 25.0 40.0 23 34.83162683474145 38.0 35.0 39.0 26.0 40.0 24 34.45631567975868 38.0 33.0 39.0 24.0 40.0 25 34.67674216134689 38.0 34.0 39.0 26.0 40.0 26 35.0021826832289 38.0 35.0 39.0 27.0 40.0 27 35.032311069146914 38.0 35.0 39.0 27.0 40.0 28 34.915709188115414 38.0 35.0 39.0 27.0 40.0 29 34.85189635933342 38.0 35.0 39.0 27.0 40.0 30 34.64331522605486 38.0 35.0 39.0 26.0 40.0 31 34.424421527633015 38.0 35.0 39.0 25.0 40.0 32 33.29211168471264 38.0 33.0 39.0 17.0 40.0 33 34.51543206091893 38.0 35.0 39.0 26.0 40.0 34 34.24925506738115 38.0 34.0 39.0 24.0 40.0 35 34.29923606086989 38.0 35.0 39.0 25.0 40.0 36 32.29190322620201 36.0 31.0 38.0 19.0 39.0 37 32.09183210506309 35.0 31.0 38.0 18.0 39.0 38 31.309879707177103 35.0 30.0 38.0 10.0 39.0 39 29.372343686772695 34.0 27.0 38.0 4.0 39.0 40 25.4973206950252 31.0 11.0 38.0 4.0 39.0 41 22.01348849186399 26.0 9.0 33.0 4.0 38.0 42 25.835697906831307 30.0 21.0 35.0 4.0 38.0 43 22.10593371019362 27.0 8.0 33.0 4.0 37.0 44 23.091623646552463 28.0 10.0 33.0 4.0 37.0 45 25.55445058920185 30.0 20.0 35.0 4.0 38.0 46 25.6848843055266 31.0 16.0 36.0 4.0 38.0 47 24.83830976934679 30.0 10.0 36.0 4.0 38.0 48 24.859032997756007 30.0 11.0 36.0 4.0 38.0 49 22.144535321455287 27.0 5.0 35.0 4.0 38.0 50 24.671371289131955 30.0 7.0 35.0 4.0 38.0 51 26.120317347426763 32.0 4.0 38.0 4.0 40.0 52 25.94489337960295 32.0 4.0 38.0 4.0 40.0 53 25.52689727900332 32.0 4.0 38.0 4.0 40.0 54 24.58918958688428 31.0 4.0 38.0 4.0 40.0 55 24.859118833613323 31.0 4.0 38.0 4.0 40.0 56 24.389412760113302 32.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.032578319615041 1 2 1.377402572645245 1 3 1.5028245187506286 1 4 2.2928092459997202 1 5 2.2315670149463926 1 6 1.6582025638925373 1 7 1.8243937443440075 1 8 1.9540469423912938 1 9 1.9246170283576944 1 10 2.0633507980877894 1 11 -4.069317712548699 1 12 -6.499695262291947 1 13 -21.401000600366075 1 14 -18.167258675141554 1 15 -11.031643191943566 1 16 -20.76326544263533 1 17 -20.54417343407261 1 18 -20.20225968200492 1 19 -5.811920867902504 1 20 -9.714534528512228 1 21 -8.173719709222379 1 22 -2.6169813909096327 1 23 -3.1398512191543624 1 24 -3.676891331611685 1 25 -3.1466556978195754 1 26 3.2631022502693696 1 27 2.93353916842365 1 28 2.635595831500396 1 29 2.3880201465298967 1 30 2.5209195377365177 1 31 0.5160421325821503 1 32 -11.223141666559886 1 33 0.2755429466720827 1 34 0.4773061863101802 1 35 0.8808254314363424 1 36 3.153268181590736 1 37 3.583483893994888 1 38 4.167918480982014 1 39 6.178299887729082 1 40 8.843822900979447 1 41 -2.5586689111221688 1 42 -8.17812141854348 1 43 -13.823530768086556 1 44 -15.970217441490057 1 45 -15.090679776858579 1 46 -18.412356218639395 1 47 -17.970795454902486 1 48 -16.725526527029047 1 49 -14.59249755750615 1 50 -16.76409468909944 1 51 -17.816530052244822 1 52 -18.246173666109737 1 53 -17.848245597154147 1 54 -18.171843291392907 1 55 -17.164804790825634 1 56 -16.84706624520791 2 1 0.7176443172151323 2 2 1.4687083433444954 2 3 1.6027481578910283 2 4 2.1955036934743575 2 5 2.6368250055649156 2 6 2.071314814356157 2 7 2.087838709981625 2 8 2.380904147583834 2 9 2.394890836582846 2 10 1.8985204282830566 2 11 -2.304836420959301 2 12 -10.959605810999268 2 13 -12.696080779268662 2 14 -14.91657688175222 2 15 -7.961718461933746 2 16 -2.413969053413119 2 17 -1.6064620781219148 2 18 -9.13037138703383 2 19 -1.169507864738982 2 20 -1.8241669055167016 2 21 2.931331016205789 2 22 3.479996900788848 2 23 2.8016781797765873 2 24 3.2170761604795146 2 25 2.6921138014713577 2 26 2.785519616910584 2 27 2.827288486630259 2 28 2.4468754213334947 2 29 3.1112665739325394 2 30 2.681495516791827 2 31 -0.299055500231205 2 32 -7.91966179310074 2 33 -0.9156864631675603 2 34 0.26305943164670964 2 35 0.7149041922086781 2 36 4.388023281405292 2 37 4.896999766144994 2 38 5.670809284952778 2 39 8.283459700099542 2 40 10.49005394712323 2 41 8.77600982783278 2 42 4.078232895845087 2 43 -1.3095130960019041 2 44 -9.760225077576017 2 45 2.025061468914302 2 46 -9.921246804436274 2 47 -10.535647642095677 2 48 -6.972935712149587 2 49 -12.227056300824575 2 50 -6.779585034905049 2 51 -6.4251261032975115 2 52 -6.766191120020505 2 53 -6.979368101790342 2 54 -14.93468827884791 2 55 -7.288291209501317 2 56 -6.908154932891993 3 1 1.248184795771813 3 2 1.7313793929584378 3 3 1.731790280052941 3 4 2.152846404276282 3 5 3.0550652012686044 3 6 2.1345362806049124 3 7 2.7507849679129777 3 8 2.7047326010659845 3 9 2.543656221492256 3 10 1.809612852409657 3 11 1.6596922847971385 3 12 1.8995792665941877 3 13 -5.102318834463219 3 14 -5.838142511291249 3 15 -2.512135096747599 3 16 2.7188189597357244 3 17 2.850765962547758 3 18 1.8918746186232305 3 19 1.237800445023268 3 20 0.7494130960122334 3 21 2.682955509746037 3 22 3.021698605551485 3 23 2.42884735644504 3 24 3.0885691656014416 3 25 2.7357648984822873 3 26 1.711213371210711 3 27 1.4409265876928643 3 28 2.378761362775279 3 29 1.9081475463352575 3 30 1.7382954556344217 3 31 2.1424245089424048 3 32 1.4651711707399429 3 33 2.204411388678629 3 34 2.435370417045384 3 35 1.9666433558811818 3 36 4.194230757897916 3 37 4.67071737183263 3 38 4.821638732242743 3 39 8.049838417322995 3 40 10.201161660600789 3 41 9.88448777294401 3 42 7.238847527753965 3 43 1.7793180331166631 3 44 3.1928366149551373 3 45 7.3879542141172685 3 46 1.1715595559337295 3 47 -3.8857737792411555 3 48 0.3991306436336082 3 49 -8.320092001459084 3 50 -0.07153519162089594 3 51 -0.8964203468568748 3 52 -0.7373706934476125 3 53 -1.6588268587661084 3 54 -5.614911503372028 3 55 -1.8168440724322856 3 56 1.3964089383716711 4 1 1.535725980783262 4 2 2.2522930021615437 4 3 2.35651804014676 4 4 2.7197755381345488 4 5 3.2977077432912765 4 6 2.8641950732558286 4 7 2.8796172681307652 4 8 3.0625814869765193 4 9 3.0384906437852166 4 10 2.714398690350123 4 11 3.1457591860089593 4 12 3.637447139493055 4 13 5.468072632560851 4 14 2.964783718674685 4 15 6.132752377424513 4 16 7.145970353045602 4 17 6.2270433959536895 4 18 6.254191031199316 4 19 5.035477331157182 4 20 5.013332233779586 4 21 4.050521266154149 4 22 4.483026577852819 4 23 3.755200179363449 4 24 4.112414589178819 4 25 3.714767523154208 4 26 2.892315733415437 4 27 3.3838539345404683 4 28 2.918885336640251 4 29 3.505400017435946 4 30 3.025501576145956 4 31 3.385513803631632 4 32 4.625611222116504 4 33 3.5753277900859004 4 34 3.765297261296233 4 35 3.1560664865004924 4 36 5.574417277136202 4 37 5.394624223901658 4 38 5.882955535727415 4 39 7.86281658228642 4 40 9.332786164984547 4 41 5.808372287379704 4 42 8.164854117355794 4 43 7.839629643101585 4 44 10.358414321997074 4 45 8.176751891003402 4 46 7.408506001457827 4 47 8.678977435367656 4 48 7.801766484366283 4 49 3.7354170173603727 4 50 7.316469499282103 4 51 6.106651077725694 4 52 6.108834701090839 4 53 5.786864770205803 4 54 0.8860099240027424 4 55 6.015847053942874 4 56 4.68221895680022 5 1 2.137948575299376 5 2 2.3376786055568672 5 3 2.550687917848215 5 4 3.207964514512504 5 5 3.031443154024508 5 6 2.914063839660031 5 7 3.1366117395665185 5 8 3.3504018924045624 5 9 3.0869633081064336 5 10 2.9434711201261905 5 11 4.084889135190842 5 12 6.828713124861906 5 13 6.732326834828118 5 14 6.909888984771744 5 15 7.511486950496494 5 16 7.60858273926328 5 17 6.90588407278398 5 18 7.143843122560238 5 19 6.009453988330357 5 20 5.859649316484678 5 21 4.588467320769659 5 22 4.352407266968804 5 23 3.838296175342677 5 24 4.32478963804239 5 25 4.2003318276161465 5 26 3.585229119242207 5 27 3.417304540448775 5 28 3.4811235674996936 5 29 3.6404310109021907 5 30 3.5650671091777255 5 31 3.6542355325644564 5 32 5.01635227310215 5 33 3.6611043020644587 5 34 3.9393074249193987 5 35 4.391840206274885 5 36 5.288718946874294 5 37 5.245520521438934 5 38 5.790142669250617 5 39 7.664011645669454 5 40 6.768090794459056 5 41 3.8766137571959796 5 42 9.577430204919317 5 43 13.842310159100915 5 44 12.673151575585056 5 45 10.010001458211754 5 46 11.486520185854989 5 47 11.8780057483179 5 48 11.509366864106052 5 49 12.727152093029108 5 50 10.700118351686193 5 51 9.253185952355729 5 52 9.294125369544517 5 53 9.183692826273266 5 54 9.361466449383876 5 55 9.647722856701574 5 56 8.05782626120632 6 1 1.4139765717998145 6 2 2.080898203107168 6 3 1.7459135146486133 6 4 2.4658072841662957 6 5 3.1972349300525096 6 6 2.443560152620911 6 7 2.500691229630263 6 8 2.642115428212591 6 9 2.598774331728478 6 10 2.1760670456354987 6 11 3.324921631128845 6 12 6.3785818912661085 6 13 6.2613857024696635 6 14 6.970693884159321 6 15 6.824072877255649 6 16 6.600208786007439 6 17 7.061052176770982 6 18 6.501235948456998 6 19 4.7318636871180075 6 20 4.851900285113601 6 21 3.6070899929356415 6 22 3.3425335011895285 6 23 3.1442579301233238 6 24 3.2731710903608473 6 25 3.270323078709758 6 26 2.693903035002741 6 27 2.913742485705619 6 28 2.437629004320087 6 29 2.16142838623027 6 30 2.3213475741196135 6 31 2.3367127229156637 6 32 3.5889681961117716 6 33 2.5432440345978904 6 34 2.31376061827023 6 35 1.8426588539439237 6 36 1.9280140349882764 6 37 1.683715747035734 6 38 2.8995039990843843 6 39 4.483721681914922 6 40 1.360141788084853 6 41 -0.4138499348425171 6 42 5.8269615135057435 6 43 10.243884962250522 6 44 8.971551775560059 6 45 6.514813356724442 6 46 8.478583677918479 6 47 8.722712659953945 6 48 8.700280499901577 6 49 10.564359942047979 6 50 8.471146973108514 6 51 8.864672016597694 6 52 9.269253478530892 6 53 8.393416610800198 6 54 8.723733665981804 6 55 8.839386398758815 6 56 7.536828885878233 7 1 1.8714644945051262 7 2 1.8398354011748026 7 3 1.6740018514326067 7 4 2.7893407555258563 7 5 2.793853287540429 7 6 2.727963189197858 7 7 2.7234655567524158 7 8 2.8814529023326685 7 9 2.720513462163261 7 10 2.22768885377905 7 11 4.11905551656708 7 12 6.214813775324075 7 13 6.418735598949997 7 14 7.1441238358501415 7 15 6.725660178842954 7 16 6.872396501673418 7 17 6.937311666073949 7 18 6.503651407394198 7 19 5.264092810651476 7 20 5.1490707475014545 7 21 3.8072280191606254 7 22 3.9356321899403923 7 23 3.9132020295022087 7 24 3.898291863307712 7 25 4.0857820159078315 7 26 2.3705766029806554 7 27 2.7182973511300474 7 28 2.6049167889991764 7 29 2.186066067389696 7 30 2.8389459178049137 7 31 2.253413896138575 7 32 3.345006843454769 7 33 1.9897588724170774 7 34 2.384292019236412 7 35 2.2147775564974097 7 36 1.0592769749468722 7 37 1.267704704937735 7 38 1.8280064145433244 7 39 4.215329687435972 7 40 -0.6564213589130752 7 41 -8.82061321986667 7 42 4.797354888246947 7 43 8.669971918772262 7 44 7.787769856995531 7 45 5.238484854308982 7 46 6.851737577159337 7 47 7.584065316958775 7 48 7.619466145174179 7 49 9.8041805079555 7 50 7.107033791604028 7 51 8.7798549013458 7 52 8.454070593782788 7 53 8.810669888923037 7 54 9.081014549349643 7 55 8.411160035749845 7 56 8.64262598732751 8 1 1.3758123205920825 8 2 2.239835401174801 8 3 2.6740018514326067 8 4 2.971949451178027 8 5 2.854722852757824 8 6 2.9975284065891614 8 7 2.9234655567524115 8 8 3.3336268153761495 8 9 3.26833954911978 8 10 2.6972540711703488 8 11 3.301664212219258 8 12 6.562639862280598 8 13 7.088300816341299 8 14 7.048471661937096 8 15 6.638703657103822 8 16 7.637613892977765 8 17 6.911224709552204 8 18 6.660173146524631 8 19 5.64670150630365 8 20 5.6186359648927535 8 21 4.1550541061171415 8 22 4.474762624722999 8 23 4.017549855589166 8 24 4.020030993742495 8 25 3.746651581125221 8 26 2.4227505160241307 8 27 3.213949525043091 8 28 2.596221136825264 8 29 2.9773704152157805 8 30 2.7606850482396936 8 31 3.3838486787472704 8 32 4.423267713019982 8 33 2.9288893071996824 8 34 2.3669007148885868 8 35 2.9017340782365437 8 36 1.1897117575555676 8 37 1.4937916614594755 8 38 2.3497455449781093 8 39 3.1109818613490177 8 40 -0.06511701108698986 8 41 -10.611917567692759 8 42 5.614746192594772 8 43 9.504754527467913 8 44 8.639943770039011 8 45 5.429789202135069 8 46 8.869128881507162 8 47 9.28841314304573 8 48 9.202074840826352 8 49 11.995484855781587 8 50 9.524425095951855 8 51 9.971159249171883 8 52 9.636679289434966 8 53 9.384582932401301 8 54 9.524492810219208 8 55 9.028551340097668 8 56 8.433930335153597 9 1 2.075087682910926 9 2 2.2336759808849465 9 3 2.477262720997821 9 4 3.1197755381345473 9 5 3.033346041163618 9 6 3.1308617399224943 9 7 3.0042626582016894 9 8 3.2028297139268744 9 9 3.240440998395144 9 10 2.628051172619628 9 11 4.249127980335196 9 12 6.478581891266103 9 13 7.120909511993471 9 14 7.256804995270432 9 15 6.433993512176283 9 16 7.2053675161661666 9 17 6.129702970421771 9 18 6.504013726234778 9 19 5.060831941086263 9 20 5.469360602573914 9 21 4.108677294522941 9 22 4.454835088491116 9 23 3.9787817396471397 9 24 3.7537266459164016 9 25 3.898100856487538 9 26 2.4673157334154396 9 27 2.782790104753232 9 28 2.8781051947962766 9 29 2.1114283862302727 9 30 2.807061859833894 9 31 3.178776214979152 9 32 4.608412640556217 9 33 3.23649800285186 9 34 2.8998717293813385 9 35 3.197023933309005 9 36 2.770871177845418 9 37 3.341255429575412 9 38 3.0856151101955014 9 39 4.730547078740322 9 40 1.0363322642753303 9 41 -3.4082943792869607 9 42 6.7781519896962195 9 43 10.602218295583857 9 44 9.258059712067997 9 45 5.945368912279999 9 46 9.653186852521653 9 47 9.994934882176164 9 48 9.912582087203166 9 49 12.122296449984486 9 50 9.208845385806928 9 51 9.380941857867533 9 52 9.636316970594386 9 53 9.641829309212895 9 54 9.882463824711959 9 55 9.83144989082231 9 56 9.245162219211569 10 1 1.9636841741389972 10 2 2.2797286124638987 10 3 2.29436798415572 10 4 2.886442204801213 10 5 2.9136091990583566 10 6 2.9168266522031985 10 7 2.762595991535022 10 8 3.0155490121724853 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33 1.067053558407416 115 34 0.6312209357305463 115 35 0.5728175841026513 115 36 3.9438870508612958 115 37 3.778953842273829 115 38 4.788392887973277 115 39 6.634515332708574 115 40 8.518871946815011 115 41 7.6178960969035145 115 42 3.295215481759712 115 43 -1.645003926638367 115 44 -1.4808291768208939 115 45 2.0386228805339677 115 46 -3.5928448935100903 115 47 -5.9677635305222445 115 48 -6.16479886517779 115 49 -7.840401962713926 115 50 -2.005043503081964 115 51 -0.20675655483087496 115 52 -0.11169890242149094 115 53 -0.9240437066601217 115 54 -0.6969012546531168 115 55 -0.025692966320551847 115 56 2.59238444143379 116 1 1.3173251258332996 116 2 1.2947490402456765 116 3 1.4728106662033014 116 4 2.1307344422441403 116 5 1.9225012923051708 116 6 1.745473612068615 116 7 1.3447877723569377 116 8 1.5944963805935402 116 9 1.5165825509065627 116 10 1.2254256018433693 116 11 0.15586314015254743 116 12 0.35346773601496295 116 13 -3.161967200335294 116 14 -7.148788612035503 116 15 -4.778449410198142 116 16 -8.966436136801867 116 17 -11.52200935834535 116 18 -3.2027214335825747 116 19 -4.205948880831549 116 20 -4.794109717060788 116 21 1.091325696349422 116 22 1.3225291524180562 116 23 1.0953342510626598 116 24 1.2122882897520242 116 25 0.9567766555742949 116 26 1.3991650484839298 116 27 2.194890561374237 116 28 1.865548117170711 116 29 2.063254870248535 116 30 2.31482441691152 116 31 0.689506808586458 116 32 -6.968870464466615 116 33 1.5251966329888447 116 34 1.2513557476461799 116 35 0.6297864903866284 116 36 3.189592639032636 116 37 3.224702918159892 116 38 3.6544507266338577 116 39 6.270958037644434 116 40 8.59432313185524 116 41 5.570929365005277 116 42 1.5611428572761277 116 43 -10.317758873365916 116 44 -2.185547593868076 116 45 -0.8154758365784502 116 46 -7.198411320994325 116 47 -9.322188405495066 116 48 -9.95671015023976 116 49 -7.04870811622556 116 50 -4.8056523310880515 116 51 -1.4926882791187666 116 52 -1.7637971846567595 116 53 -4.022325941928656 116 54 -6.106120650173885 116 55 -3.173116319223354 116 56 -2.3649975981400324 117 1 0.38473680571794233 117 2 0.4025356300077547 117 3 0.9259469315241375 117 4 1.614512380239809 117 5 1.6153635850232675 117 6 0.8027915644839041 117 7 0.7362802020613373 117 8 1.0944963805935402 117 9 1.3093006475179507 117 10 0.8732266112161184 117 11 -10.369731668787608 117 12 -10.244225126277755 117 13 -9.560669435374948 117 14 -10.61994939069448 117 15 -8.742760873788626 117 16 -12.178404413658392 117 17 -10.592665450630857 117 18 -3.4929160983266243 117 19 -4.803641743124267 117 20 -4.321867467601525 117 21 0.3999053646983768 117 22 1.0175543867367267 117 23 0.23448349403309976 117 24 -2.5923259856625442 117 25 -0.03961844175807272 117 26 1.9186315228891218 117 27 0.6819129117707803 117 28 1.59301747549803 117 29 0.8894985616688658 117 30 1.536886421237405 117 31 0.39895165357564366 117 32 -11.896411920847292 117 33 0.4325506344308039 117 34 0.12662611534625512 117 35 0.19395375787041047 117 36 2.1322746866173468 117 37 1.559237885715767 117 38 3.194825636511286 117 39 5.801599710319266 117 40 5.643349808134978 117 41 4.238196848783215 117 42 -0.9687778348651825 117 43 -8.677167669328425 117 44 -6.241063094949547 117 45 -1.5173503859656172 117 46 -5.750321919408169 117 47 -10.344538802034359 117 48 -7.835224930340695 117 49 -9.3399842517699 117 50 -4.475803737000092 117 51 -1.925637089500885 117 52 -1.8878058364231585 117 53 -3.715188234646753 117 54 -6.784202841954706 117 55 -3.6860939688268175 117 56 -1.3754518158761506 118 1 0.7155638733871115 118 2 0.8884378856468516 118 3 0.9879770067121072 118 4 1.809061252420264 118 5 1.7488222316398065 118 6 1.1618141208748796 118 7 1.3518817058207375 118 8 1.6837820948792555 118 9 1.8487743317284782 118 10 1.511384505952961 118 11 -7.81991963871242 118 12 -10.990465727781512 118 13 -10.214804773720811 118 14 -0.3229569094914737 118 15 -15.731482678299905 118 16 -6.014870579071925 118 17 -11.002439886721085 118 18 -3.0305100832890304 118 19 -3.8534537731994547 118 20 -2.8425441593308456 118 21 0.8491534849991282 118 22 0.792930326586351 118 23 0.9299722158376156 118 24 -2.653416211226453 118 25 1.542148475535157 118 26 1.8923157334154368 118 27 1.7411234380865679 118 28 1.8364385281296123 118 29 1.9054760052778903 118 30 1.2380142407862778 118 31 -0.37360473740179856 118 32 -11.847539740396165 118 33 -0.006359140005287145 118 34 0.3474907770003881 118 35 -0.25059511431004466 118 36 3.666109273083517 118 37 3.532922096242082 118 38 4.814186538766929 118 39 6.166261364454606 118 40 8.473237026180094 118 41 6.546467525474945 118 42 0.850771037315269 118 43 0.15459924796480706 118 44 -1.8717021926939097 118 45 3.8679879598990468 118 46 -3.995622671287869 118 47 -9.71577940353812 118 48 -7.635036960415883 118 49 -6.832465454777418 118 50 -3.52091651895498 118 51 -1.4547724278467484 118 52 -0.7684449341675226 118 53 -3.2772183098347227 118 54 -7.355631413383277 118 55 -4.144740585368169 118 56 -0.207218733169384 119 1 0.7220573798806242 119 2 1.38291840512737 119 3 1.6068081755432786 119 4 2.047048265407277 119 5 2.3416793744969553 119 6 1.4520738611346218 119 7 1.3898687188077474 119 8 1.121769107866264 119 9 1.5624106953648393 119 10 0.9818390514075048 119 11 -8.486478080270864 119 12 -10.236569623885408 119 13 -2.656363215279253 119 14 -3.3151647016992634 119 15 -5.3288852757025005 119 16 -9.536299150500497 119 17 -11.463478847760044 119 18 -1.7421983949773434 119 19 -2.232349877095558 119 20 -2.145790912577599 119 21 0.33291971876536763 119 22 0.869229027885055 119 23 -0.5128849270195275 119 24 -7.2621824449926855 119 25 0.617797826184507 119 26 2.6014066425063476 119 27 2.211577983541112 119 28 2.7614385281296094 119 29 2.3402162650181495 119 30 1.52827398104602 119 31 0.516655002857938 119 32 -9.405981298837723 119 33 0.802407093760948 119 34 1.1036596081692167 119 35 1.5705087817938548 119 36 4.7557196626939024 119 37 4.523831187151174 119 38 4.919706019286405 119 39 7.48963798783123 119 40 8.751483779426842 119 41 8.78412986313728 119 42 3.0637580503022797 119 43 2.3347940531596123 119 44 1.1838172878255726 119 45 3.53097497288606 119 46 -5.204388905054103 119 47 -8.209610572369288 119 48 -11.34575124613017 119 49 -5.051945974257938 119 50 -4.7222152202536805 119 51 -6.139512687587008 119 52 -7.717470908193494 119 53 -10.768776751393165 119 54 -9.284202841954706 119 55 -10.883701624329207 119 56 -6.732868083818735 120 1 0.6465566094645965 120 2 0.8445516870996386 120 3 1.0709067887944315 120 4 1.77062299576167 120 5 2.1247302219545787 120 6 0.9432911184535726 120 7 0.7541214152638389 120 8 0.7300896009325228 120 9 1.0902997554572949 120 10 0.7380794212071962 120 11 -9.311747725879492 120 12 -0.6750904251180749 120 13 -4.691802352413305 120 14 -5.666782575351036 120 15 -7.789594058445182 120 16 -11.437994065754737 120 17 -10.848686860086701 120 18 -2.4639241268725662 120 19 -2.3870494148459436 120 20 -3.0453286629628096 120 21 1.1572648651444126 120 22 1.4270102297340514 120 23 0.3718608356923312 120 24 -4.391612337134443 120 25 0.4492307999903673 120 26 2.3668920046018798 120 27 2.340699709273011 120 28 2.3004215789770726 120 29 2.571032906004284 120 30 2.663559034975137 120 31 -0.7616192652468357 120 32 -10.455287924415533 120 33 -0.8274850479956015 120 34 -7.801116971183632 120 35 -0.14012295934636398 120 36 1.2121141156985296 120 37 1.1007187064115769 120 38 1.0275642627378723 120 39 2.4822419939945597 120 40 1.2035639026934106 120 41 3.4241914964192546 120 42 -2.2702943379873943 120 43 0.4303256402166227 120 44 -1.9761210788924544 120 45 -8.053924873030736 120 46 -10.259242526009418 120 47 -11.029641389010274 120 48 -9.427107178333559 120 49 -14.506799595213254 120 50 -8.364742184814542 120 51 -7.861854752301955 120 52 -7.605914667823695 120 53 -8.563537922425523 120 54 -8.65708419788691 120 55 -8.637476662850009 120 56 -4.449046820336456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1674.0 5 267.0 6 13.0 7 121.0 8 250.0 9 240.0 10 126.0 11 111.0 12 133.0 13 146.0 14 220.0 15 304.0 16 385.0 17 483.0 18 654.0 19 754.0 20 900.0 21 1129.0 22 1440.0 23 1715.0 24 1931.0 25 2209.0 26 2311.0 27 2432.0 28 2686.0 29 2798.0 30 3094.0 31 3661.0 32 4567.0 33 5464.0 34 6490.0 35 7597.0 36 8864.0 37 9022.0 38 5678.0 39 1682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.66401392993342 0.19742247182744538 0.12262265330897229 0.0159409449301664 8 99.30595578227121 0.5419921276256576 0.08583585731628061 0.06621623278684503 9 97.53093991095193 1.2756197181371045 0.5580836266849832 0.635356744225981 10 44.521832963421666 37.434243602163065 7.930006989491238 10.113916444924035 11 28.244901963188678 31.695503427303162 22.82743313999828 17.232161469509876 12 40.257017081335604 13.661389805152604 19.684614535689324 26.396978577822466 13 25.05180807102304 16.378707802479433 25.11802430380989 33.451459822687646 14 27.09978903988618 16.44262375509003 24.513074621007704 31.944512584016096 15 25.792822226043384 20.32842860301162 22.877005084870667 31.001744086074336 16 27.108891942290708 19.703847286289136 28.34061242516439 24.846648346255765 17 32.435979199372056 22.282182103610676 21.835753532182103 23.446085164835164 18 31.416238488233777 23.555740861098506 23.15842397635719 21.86959667431052 19 27.555588260544294 29.483547346603384 18.74118498350442 24.2196794093479 20 24.30509555702755 25.38823875763598 20.698707097470624 29.607958587865852 21 23.542686142487458 25.397059003887808 28.951273654905137 22.108981198719597 22 24.235735570025383 27.10762854234877 24.195269102012286 24.46136678561356 23 26.838420129734768 30.968351093180956 22.290345918504983 19.902882858579293 24 25.153584873269487 30.255913477455827 21.65148189476524 22.93901975450945 25 27.27005186938235 27.553310198526077 22.652082745766453 22.52455518632512 26 24.31930997198604 24.283678183515995 30.036368997886665 21.360642846611295 27 26.46990272206479 19.654313718274267 28.35044590831585 25.525337651345087 28 25.01965698560126 27.259324782544596 26.138876603272887 21.582141628581258 29 27.125858684985282 25.54833169774289 27.833660451422965 19.492149165848872 30 24.185099309312623 24.28815035638487 33.263405837105736 18.263344497196766 31 23.840489741730075 25.069761152564872 33.23335259191877 17.85639651378628 32 29.175711640114304 21.283589045464023 30.301580877393086 19.239118437028587 33 23.7043862882418 26.81041897038948 31.636564688536676 17.848630052832043 34 21.10689693265088 25.254384995888106 35.28372058769378 18.354997483767228 35 24.312286815047486 27.627542882383256 33.386910750656426 14.673259551912837 36 22.400471692319034 28.84324828950116 31.834318072940338 16.92196194523947 37 22.32002360346184 27.057926829268293 33.58330055074744 17.038749016522424 38 22.616299061747803 29.094414697647004 34.337083067249594 13.952203173355601 39 20.81994459833795 26.123730378578024 27.874422899353647 25.181902123730378 40 12.596093335472169 17.969916462037734 23.786725237842575 45.64726496464752 41 16.44905384834346 20.231185257608196 26.13086471702083 37.188896177027516 42 17.13832806989518 28.758033399279913 34.002629671045355 20.10100885977955 43 11.116169757226002 17.434692195055927 26.946314092704476 44.502823955013596 44 12.615854860974165 18.170725695129168 23.746549004141198 45.466870439755475 45 18.14655225328079 30.658368013356412 33.6721540897875 17.522925643575295 46 21.06832572522456 28.37088303146321 33.676925342364896 16.88386590094733 47 16.508236333083932 25.327184192127955 34.33011566436483 23.834463810423284 48 18.20022083179978 28.1278370752055 37.65059501901607 16.021347073978653 49 13.617858680277967 18.37279380111924 24.290019064018203 43.71932845458459 50 16.9231336496153 28.193751382709376 38.76256729185615 16.12054767581918 51 21.5053101810261 28.183955008060646 33.82640692107951 16.484327889833743 52 16.876577408433363 31.77100646352724 33.40104647583872 17.951369652200675 53 20.278388638401907 30.35615563227146 33.69534503728639 15.670110692040248 54 14.940877445021549 26.25827286010732 33.37016981618595 25.43067987868518 55 16.09116361314406 28.67773466280269 34.315290534898566 20.915811189154674 56 21.017261502549296 28.65900853860802 32.05602309724185 18.267706861600836 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 2.5 7 3.0 8 23.0 9 43.0 10 76.0 11 109.0 12 155.0 13 251.0 14 301.0 15 379.5 16 458.0 17 469.0 18 480.0 19 626.0 20 772.0 21 962.0 22 1455.5 23 1759.0 24 2095.0 25 2431.0 26 2704.0 27 2977.0 28 3191.5 29 3310.5 30 3215.0 31 3032.5 32 2850.0 33 2643.5 34 2437.0 35 2301.0 36 2165.0 37 2060.5 38 1919.0 39 1882.0 40 1896.0 41 1910.0 42 2104.5 43 2299.0 44 2593.5 45 2888.0 46 3108.5 47 3400.5 48 3472.0 49 3525.0 50 3578.0 51 3702.5 52 3827.0 53 3852.0 54 4002.0 55 4127.0 56 4257.5 57 4388.0 58 4407.5 59 4427.0 60 4294.5 61 4162.0 62 3750.0 63 2853.0 64 2368.0 65 2091.0 66 1814.0 67 1566.5 68 1319.0 69 1055.0 70 791.0 71 645.0 72 396.0 73 293.0 74 220.5 75 148.0 76 104.5 77 61.0 78 43.5 79 16.5 80 7.0 81 5.0 82 3.0 83 1.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02697698372797391 10 0.0 11 0.0 12 0.0 13 0.0 14 0.023298304128704737 15 0.16308812890093316 16 0.047822834790499195 17 0.018393397996345845 18 0.004904906132358892 19 0.017167171463256123 20 0.03556056945960197 21 0.017167171463256123 22 0.002452453066179446 23 0.0 24 0.0 25 0.0 26 0.19987492489362485 27 0.039239249058871134 28 0.19006511262890707 29 0.03801302252578141 30 0.04659660825740947 31 0.24769775968412405 32 0.017167171463256123 33 0.19742247182744538 34 0.09809812264717784 35 0.06008510012139643 36 0.17412416769874067 37 0.2538288923495727 38 0.15082586357003594 39 0.3997498497872497 40 0.6253755318757587 41 0.9196698998172923 42 0.21091096369143233 43 0.3457958823313019 44 0.508884011232235 45 0.11281284104425451 46 0.07234736545229366 47 0.02697698372797391 48 0.050275287856678647 49 0.3016517271400719 50 0.23175681475395762 51 0.3580581476621991 52 0.3997498497872497 53 0.18883888609581734 54 0.13488491863986954 55 0.14101605130531814 56 0.19129133916199678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 81551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.93640789199397 #Duplication Level Percentage of deduplicated Percentage of total 1 93.75017836248965 80.56553567706098 2 4.120886960988556 7.082684455126239 3 0.9759995433920264 2.5162168459001117 4 0.41094717616506377 1.412612966119361 5 0.1812162894894552 0.7786538485119742 6 0.11272509346194459 0.5812313766845287 7 0.08704089495162809 0.5235987296293116 8 0.054222196855112585 0.3727728660592758 9 0.04423389743443395 0.3421172027320327 >10 0.22687708684112898 3.4150408946548785 >50 0.027111098427556293 1.572022415421025 >100 0.00856139950343883 0.8375127221002807 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAGTTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 125 0.15327831663621536 No Hit AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 124 0.15205209010312565 No Hit AAAGTTGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 118 0.14469473090458732 No Hit AAAGTTGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 110 0.13488491863986954 No Hit AAAGTTGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 104 0.1275275594413312 No Hit AAAGTTGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 102 0.12507510637515176 No Hit AAAGTTGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 97 0.11894397370970312 No Hit AAAGTTGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 88 0.10790793491189564 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.001226226533089723 0.0 0.0 0.0 0.0 9 0.001226226533089723 0.0 0.0 0.0 0.0 10 0.017167171463256123 0.0 0.0 0.0 0.0 11 0.030655663327243076 0.0 0.0 0.0 0.0 12 0.041691702125050585 0.0 0.0 0.0 0.0 13 0.04537038172431975 0.0 0.0 0.0 0.0 14 0.05150151438976837 0.0 0.0 0.0 0.0 15 0.05640642052212726 0.0 0.0 0.0 0.0 16 0.06008510012139643 0.0 0.0 0.0 0.0 17 0.06621623278684505 0.0 0.0 0.0 0.0 18 0.07234736545229366 0.0 0.0 0.0 0.0 19 0.07970472465083199 0.0 0.0 0.0 0.0 20 0.08583585731628061 0.0 0.0 0.0 0.0 21 0.09196698998172922 0.0 0.0 0.0 0.0 22 0.09809812264717784 0.0 0.0 0.0 0.0 23 0.10790793491189563 0.0 0.0 0.0 0.0 24 0.11649152064352368 0.0 0.0 0.0 0.0 25 0.12384887984206203 0.0 0.0 0.0 0.0 26 0.1324324655736901 0.0 0.0 0.0 0.0 27 0.13611114517295925 0.0 0.0 0.0 0.0 28 0.14592095743767702 0.0 0.0 0.0 0.0 29 0.15205209010312565 0.0 0.0 0.0 0.0 30 0.1545045431693051 0.0 0.0 0.0 0.0 31 0.15695699623548454 0.001226226533089723 0.0 0.0 0.0 32 0.15818322276857427 0.001226226533089723 0.0 0.0 0.0 33 0.15818322276857427 0.001226226533089723 0.0 0.0 0.0 34 0.15818322276857427 0.001226226533089723 0.0 0.0 0.0 35 0.15818322276857427 0.001226226533089723 0.0 0.0 0.0 36 0.15818322276857427 0.001226226533089723 0.0 0.0 0.0 37 0.15940944930166398 0.001226226533089723 0.0 0.0 0.0 38 0.15940944930166398 0.001226226533089723 0.0 0.0 0.0 39 0.16063567583475372 0.001226226533089723 0.0 0.0 0.0 40 0.16063567583475372 0.001226226533089723 0.0 0.0 0.0 41 0.16431435543402287 0.001226226533089723 0.0 0.0 0.0 42 0.16799303503329205 0.001226226533089723 0.0 0.0 0.0 43 0.17535039423183038 0.001226226533089723 0.0 0.0 0.0 44 0.18516020649654819 0.001226226533089723 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCCCA 80 0.0 50.418736 39 CTGACCA 15 0.008130303 50.418736 39 CCCACGT 15 0.008130303 50.418736 39 TACGCCC 80 0.0 50.418736 38 GCCAACC 15 0.008130303 50.418736 39 CTGAGAT 15 0.0081702825 50.35626 41 TGGCCTT 15 0.0081702825 50.35626 37 AAACCGC 15 0.0081702825 50.35626 37 GTACGCC 90 0.0 50.35626 37 GAGTCCG 15 0.008230527 50.262833 36 ATACCCA 15 0.008230527 50.262833 36 CCGAAAC 20 4.1167377E-4 50.107895 43 CCAATAC 15 0.008331668 50.10789 43 CCAACAC 90 0.0 50.10789 43 CATGACT 15 0.008352007 50.07702 44 ATTATAT 20 4.1292905E-4 50.07702 44 TCTGATA 15 0.008372382 50.046185 48 GCCCAAA 15 0.008372382 50.046185 48 AATCAAT 15 0.008372382 50.046185 49 CAAAAAG 15 0.008372382 50.046185 49 >>END_MODULE