FastQCFastQC Report
Fri 17 Jun 2016
SRR1526337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301960
Sequences flagged as poor quality0
Sequence length56
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAACCAAAAAAAAAAAAAAA9140.30268909789376075No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAACCAAAAAAAAAAAAAAA6980.23115644456219367No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAACCAAAAAAAAAAAAAAA6570.2175784872168499No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAACCAAAAAAAAAAAAAAA6520.21592263876010065No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAACCAAAAAAAAAAAAAAA5530.18313683931646577No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAACCAAAAAAAAAAAAAAA4580.15167571863823023No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAACCAAAAAAAAAAAAAAA4580.15167571863823023No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAACCAAAAAAAAAAAAAAA4010.1327990462312889No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA4010.1327990462312889No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAACCAAAAAAAAAAAAAAA3940.13048085839183998No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAACCAAAAAAAAAAAAAAA3940.13048085839183998No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA3910.12948734931779043No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAACCAAAAAAAAAAAAAAA3740.12385746456484302No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAACCAAAAAAAAAAAAAAA3720.12319512518214333No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAACAAAAAAAAAAAAAAA3500.1159093919724467No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAACCAAAAAAAAAAAAAAA3100.10266260431845277No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAACAAAAAAAAAAAAAAA3040.10067558617035369No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAACCAAAAAAAAAAAAAAA3030.10034441647900384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCA150.0081036250.49428639
ACTGCTT309.766864E-750.49428640
TCGGCGA203.9808798E-450.49428639
CCGTCCA150.0081036250.49428639
AAGCCCG150.0081036250.49428640
CCCGAAT203.9808798E-450.49428639
ACCTCCC203.9841823E-450.48580638
TGAAACC150.00810900450.485838
ACCGTCC150.00810900450.485838
ACCCGAA150.00810900450.485838
AACCGAT204.0040415E-450.43497541
CACCATC150.00814136550.4349741
AACGAAC204.0239797E-450.38424337
AACCCAT150.008173821550.3842437
CTTGAAC309.915002E-750.3842437
ACGCGCA150.00821724650.31675736
GATACCC402.5520421E-950.2578635
ACTTTCC301.0088297E-650.2578635
GCCCGGA150.00832106850.15720742
TGCGGCA150.00833205450.14047247
Produced by FastQC (version 0.11.5)