##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526337_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 301960 Sequences flagged as poor quality 0 Sequence length 56 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.982252616240565 39.0 37.0 40.0 33.0 40.0 2 36.022516227314874 39.0 36.0 40.0 29.0 40.0 3 35.60673599152206 39.0 35.0 40.0 27.0 40.0 4 35.47509603921049 39.0 35.0 40.0 27.0 40.0 5 35.936789641012055 39.0 36.0 40.0 29.0 40.0 6 36.15467280434495 39.0 36.0 40.0 30.0 40.0 7 36.16029606570407 38.0 36.0 40.0 30.0 40.0 8 35.93161014703934 38.0 36.0 40.0 30.0 40.0 9 35.915564975493446 38.0 36.0 40.0 30.0 40.0 10 35.229934428401116 38.0 35.0 39.0 27.0 40.0 11 33.71251490263611 37.0 32.0 39.0 24.0 40.0 12 30.939031659822493 35.0 27.0 39.0 11.0 40.0 13 30.35840508676646 35.0 26.0 39.0 10.0 40.0 14 30.77710292754007 36.0 26.0 39.0 11.0 40.0 15 31.418737581136575 35.0 28.0 39.0 13.0 40.0 16 31.00305007285733 35.0 27.0 39.0 12.0 40.0 17 31.05612663928997 35.0 27.0 39.0 13.0 40.0 18 31.186061067691085 35.0 27.0 38.0 15.0 40.0 19 32.184994701284936 36.0 29.0 39.0 19.0 40.0 20 32.23888263346139 36.0 29.0 39.0 19.0 40.0 21 34.21422042654656 38.0 33.0 39.0 23.0 40.0 22 34.02755994171413 38.0 33.0 39.0 23.0 40.0 23 34.083279242283744 38.0 33.0 39.0 23.0 40.0 24 33.79974831103458 38.0 33.0 39.0 22.0 40.0 25 33.93765730560339 38.0 33.0 39.0 22.0 40.0 26 34.69646310769638 38.0 35.0 39.0 25.0 40.0 27 34.60058285865678 38.0 35.0 39.0 25.0 40.0 28 34.52009868856802 38.0 35.0 39.0 25.0 40.0 29 34.3869717843423 38.0 35.0 39.0 24.0 40.0 30 34.20850112597695 38.0 34.0 39.0 23.0 40.0 31 33.58326930719301 38.0 33.0 39.0 19.0 40.0 32 32.35666313418996 37.0 32.0 39.0 10.0 40.0 33 33.2981123327593 38.0 33.0 39.0 18.0 40.0 34 32.97963968737581 37.0 33.0 39.0 15.0 40.0 35 33.04183666710823 38.0 33.0 39.0 11.0 40.0 36 30.421350510001325 35.0 29.0 38.0 4.0 39.0 37 30.22483110345741 35.0 29.0 38.0 4.0 39.0 38 29.658537554643 34.0 28.0 38.0 4.0 39.0 39 27.91208107034044 32.0 24.0 37.0 4.0 39.0 40 24.973519671479668 31.0 10.0 36.0 4.0 39.0 41 21.830156312094317 26.0 7.0 33.0 4.0 37.0 42 24.748082527487085 30.0 17.0 34.0 4.0 38.0 43 21.699629089945688 26.0 6.0 33.0 4.0 37.0 44 22.83495827261889 28.0 7.0 34.0 4.0 37.0 45 24.379805272221486 30.0 11.0 35.0 4.0 38.0 46 24.480921314081336 30.0 4.0 36.0 4.0 38.0 47 23.604785402040005 29.0 4.0 35.0 4.0 38.0 48 23.32784805934561 29.0 4.0 35.0 4.0 38.0 49 21.39298582593721 26.0 4.0 35.0 4.0 38.0 50 22.91683997880514 28.0 4.0 35.0 4.0 38.0 51 23.86606504172738 31.0 4.0 36.0 4.0 40.0 52 23.605881573718374 30.0 4.0 36.0 4.0 39.0 53 23.22121141873096 30.0 4.0 36.0 4.0 39.0 54 22.520867002251954 29.0 4.0 35.0 4.0 39.0 55 22.401450523248112 29.0 4.0 35.0 4.0 39.0 56 23.152771890316597 31.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4871140299592014 1 2 2.4941827071325022 1 3 2.871532816536721 1 4 2.8169489724617165 1 5 2.4863660542592 1 6 2.568088883278776 1 7 2.597642309074864 1 8 2.7205879840418135 1 9 2.697306806262972 1 10 2.33476843913634 1 11 -4.636185583830738 1 12 -6.813535611203832 1 13 -19.32791427017341 1 14 -17.54104454714625 1 15 -12.11444053916103 1 16 -19.83449189999662 1 17 -19.68001406850063 1 18 -19.824070610329624 1 19 -6.53152259963624 1 20 -10.305331620518956 1 21 -8.434551225311758 1 22 -3.6721288292990764 1 23 -3.773757118197018 1 24 -3.643281613448817 1 25 -3.812117082164189 1 26 2.2100293335516454 1 27 2.0462449151926165 1 28 1.8803352393070227 1 29 1.8990868825487226 1 30 2.067025871034595 1 31 1.1736896506574652 1 32 -12.336274908242267 1 33 -0.22653553504466117 1 34 0.045306727010341774 1 35 -0.13207500050004128 1 36 1.4850174536732936 1 37 1.435764191342983 1 38 2.0000286784080075 1 39 3.1835053009614924 1 40 4.044150037257161 1 41 -11.082318077325574 1 42 -14.13822212625353 1 43 -15.28101396663536 1 44 -17.087525810374444 1 45 -17.589096049675238 1 46 -18.721786354764294 1 47 -17.855357128273976 1 48 -17.080745013005906 1 49 -15.313431357523886 1 50 -16.73500915355644 1 51 -17.47705987807742 1 52 -17.11255341122905 1 53 -16.91579110359001 1 54 -16.608536441015037 1 55 -16.108875285490164 1 56 -16.76246162831279 2 1 1.462664770298673 2 2 2.6163206630732745 2 3 2.9642377641712443 2 4 2.6780179504321 2 5 2.5968391492393366 2 6 2.5096562361137345 2 7 2.3420626774388893 2 8 2.5707143834639865 2 9 2.4371767954287336 2 10 2.4708103315162617 2 11 -3.573130329226185 2 12 -12.163409211781655 2 13 -12.624591771076265 2 14 -15.174775135806417 2 15 -6.868106122404079 2 16 -4.281223572080403 2 17 -2.0056189800210333 2 18 -10.36462676778719 2 19 -1.9653254165376488 2 20 -3.0128433576081584 2 21 3.1027546612899073 2 22 3.4249459269306115 2 23 3.104971664757116 2 24 3.3643023518816264 2 25 3.124358179663183 2 26 2.8112933277733845 2 27 2.8258404370416628 2 28 2.813849143243459 2 29 3.0549554271496646 2 30 3.0595863621866357 2 31 -3.892796445406095 2 32 -11.47271405594902 2 33 -3.35755756465824 2 34 -2.316195620407502 2 35 -2.512934516570823 2 36 4.284403513622738 2 37 4.138942233957643 2 38 4.96427569899306 2 39 6.685094322268824 2 40 7.801029777235492 2 41 3.0182236344837428 2 42 0.33917043423762294 2 43 -1.5595621789863898 2 44 -11.215080884408394 2 45 -0.9574961941317177 2 46 -15.115935867223666 2 47 -15.046978652289866 2 48 -12.774894525465276 2 49 -13.654131971463936 2 50 -12.538114967929861 2 51 -11.980562947777674 2 52 -11.817284361030424 2 53 -11.558730793008573 2 54 -14.218648394788962 2 55 -11.143797640130828 2 56 -11.55816404795887 3 1 1.7959981036320087 3 2 2.5326499478236073 3 3 2.7337384389350774 3 4 2.6904336049530215 3 5 2.279295289590216 3 6 2.61559415784113 3 7 2.455827859625124 3 8 2.6592433983087886 3 9 2.6420350950238785 3 10 2.530594407089808 3 11 1.4224162294782658 3 12 1.763581341524688 3 13 -5.378168019389353 3 14 -6.057501181690359 3 15 -3.0322086865066424 3 16 3.004066576367638 3 17 3.037565905269112 3 18 2.4113516397701638 3 19 1.6047150692923076 3 20 1.780948815131385 3 21 3.9393268610199996 3 22 4.225350785230205 3 23 3.8858083719096115 3 24 4.183735550262192 3 25 4.122603793698275 3 26 3.1751259863428842 3 27 3.162952447837881 3 28 2.8654011000585697 3 29 3.4847799885531714 3 30 2.572271922240617 3 31 3.1794032846883695 3 32 1.7526570641589387 3 33 2.827327725490207 3 34 3.3790810327638923 3 35 2.910007453739567 3 36 5.063890693109915 3 37 5.532194595631061 3 38 5.691806063365529 3 39 7.442854106344399 3 40 8.67390427116262 3 41 8.826725658775242 3 42 7.274528059068931 3 43 3.893474258260575 3 44 3.141869183067989 3 45 6.782854683061263 3 46 -0.6705917376690103 3 47 -4.646438841223738 3 48 0.345753247814077 3 49 -10.995697423555704 3 50 -3.2476966143536146 3 51 -4.7296857547952165 3 52 -4.407432809073608 3 53 -4.3661531951678185 3 54 -5.0633177605109605 3 55 -3.6755115402657808 3 56 -1.2878536565958463 4 1 2.166365739834575 4 2 2.913333311333524 4 3 3.4618656001867976 4 4 3.4046432764816146 4 5 3.1423039851072403 4 6 3.246897242068755 4 7 3.0764344477031784 4 8 3.275739237049301 4 9 3.3944926082147973 4 10 3.3307296188827635 4 11 4.285514289111333 4 12 5.0594659297115285 4 13 6.135430867833133 4 14 3.730810769011736 4 15 6.548553162009522 4 16 7.417363956833423 4 17 7.0933524260521 4 18 6.565586333251247 4 19 5.720663067147573 4 20 5.32503252211033 4 21 4.097567398137187 4 22 4.236816589856936 4 23 3.9356424654855005 4 24 4.654661129380521 4 25 4.093416696322471 4 26 3.1918681592119427 4 27 3.394751308639677 4 28 2.8984153128384165 4 29 3.06903092299099 4 30 3.1577506419248067 4 31 3.323823466991172 4 32 3.8536307444840716 4 33 2.822470980053609 4 34 3.11157149411585 4 35 2.6869079953638177 4 36 4.696328313071529 4 37 4.597581930793119 4 38 5.402899647021993 4 39 7.165949679323795 4 40 8.265284907901375 4 41 8.100137114477839 4 42 7.69558069064788 4 43 8.547052319758627 4 44 9.949001993686 4 45 7.987968641736188 4 46 7.809651021894894 4 47 7.646667688573366 4 48 7.459521538317389 4 49 0.0756578803988539 4 50 7.557007823546098 4 51 4.7363325687339675 4 52 4.891598950156151 4 53 4.690410404885025 4 54 3.735601322224671 4 55 4.44380509622431 4 56 4.811721281043543 5 1 2.6974507282605984 5 2 3.6832294338447014 5 3 4.053089970620022 5 4 4.070270619079622 5 5 3.73537772082512 5 6 3.7459828565605306 5 7 3.735162710453089 5 8 3.8013955763769403 5 9 3.7180285616882287 5 10 3.5323928119828594 5 11 5.177711532774474 5 12 8.931649663534728 5 13 8.792124416884558 5 14 7.897999157139548 5 15 8.104800227326304 5 16 8.953980565698838 5 17 8.657911338015722 5 18 8.259946798846464 5 19 7.290897759428017 5 20 7.1177718069125895 5 21 5.099046066594184 5 22 5.106986204249857 5 23 5.016075439380202 5 24 5.5575344416802395 5 25 5.146191568020178 5 26 4.2460022526110635 5 27 4.199076928733092 5 28 3.9794043319746137 5 29 4.343939690382854 5 30 4.389156077164074 5 31 4.2809132497628255 5 32 5.592268886716326 5 33 4.223437263117702 5 34 4.4403136785910675 5 35 3.998433371621097 5 36 5.714105285384594 5 37 5.877062625726104 5 38 6.508811849537789 5 39 7.335914174805417 5 40 2.4054206653173686 5 41 7.074975037587077 5 42 9.277125755025565 5 43 11.789166774463592 5 44 10.852213051984254 5 45 8.917952248698697 5 46 10.247880573845997 5 47 10.578101132017384 5 48 10.85372878255717 5 49 10.273820047904394 5 50 10.724006744406442 5 51 9.61928298597384 5 52 9.81278056688858 5 53 9.735964926150665 5 54 9.029860464020327 5 55 9.62669867328394 5 56 8.629202519665206 6 1 2.343819751011175 6 2 3.382771763595173 6 3 3.5063639270938722 6 4 3.4902258015780063 6 5 3.429298872407024 6 6 3.5119253534271024 6 7 3.2440027727418226 6 8 3.3414654612947103 6 9 3.2872108321884355 6 10 3.1605879580195477 6 11 4.787352992761562 6 12 8.644067529765884 6 13 8.023456788034242 6 14 8.380319032562088 6 15 7.779227664752714 6 16 8.120194626240451 6 17 7.907303643078585 6 18 7.554422472058512 6 19 6.337329450718986 6 20 6.4799295037487745 6 21 4.398715632472111 6 22 4.513543612430951 6 23 4.22000456695816 6 24 4.83591569429143 6 25 4.498683117262459 6 26 3.6210164954611557 6 27 3.515277695947944 6 28 3.265153885698645 6 29 3.6309141292145597 6 30 3.3521436573987984 6 31 3.6505436952791754 6 32 4.625198356122681 6 33 3.427727209564587 6 34 3.555633288147071 6 35 3.0688785081625554 6 36 3.8360145869152227 6 37 3.535652013853266 6 38 4.153281825609806 6 39 5.325042132570616 6 40 4.2575988718576845 6 41 5.529217507866999 6 42 6.553987770293897 6 43 10.203405826459491 6 44 8.326670874157525 6 45 6.584698042310304 6 46 8.743151947616361 6 47 8.48952009969561 6 48 8.579326032737583 6 49 8.496321851998736 6 50 8.603455261250986 6 51 8.784538027942773 6 52 8.965198053310399 6 53 9.240883457048156 6 54 9.252238830080564 6 55 8.63144628998036 6 56 7.450027465542377 7 1 2.289068096702003 7 2 3.4454959922486026 7 3 3.690052039985524 7 4 3.634947957362108 7 5 3.5137483661485547 7 6 3.407265383722887 7 7 3.1817369671131814 7 8 3.3098342725838776 7 9 3.2843008425527813 7 10 2.945966256672186 7 11 4.8572924011965455 7 12 8.474809776437333 7 13 8.766122019637525 7 14 8.55890469787342 7 15 7.742912588614633 7 16 8.259455568598735 7 17 8.22064574624369 7 18 7.670543017382599 7 19 6.54503494382271 7 20 6.752471957707154 7 21 4.515055423590667 7 22 4.9163180683027505 7 23 4.627702087487535 7 24 4.749333536912808 7 25 4.393484998790001 7 26 3.613303029783083 7 27 3.494170305371533 7 28 3.528228907623223 7 29 3.5549554271496646 7 30 3.4013743039745776 7 31 3.5968085441988933 7 32 4.255629672394704 7 33 3.2864133293126514 7 34 3.6991196949078144 7 35 3.350509696873388 7 36 1.6742510534702753 7 37 1.4348188798342925 7 38 1.6540192887366487 7 39 3.375149762324263 7 40 1.493268169473886 7 41 -10.178727162467053 7 42 4.7834185284857185 7 43 7.970825901401689 7 44 6.260837341509834 7 45 4.372926536291011 7 46 5.817536066248266 7 47 5.833755928444713 7 48 6.261280110709361 7 49 7.525840308508343 7 50 6.092931463116571 7 51 8.55128043406571 7 52 8.242902749156688 7 53 8.417256732978952 7 54 8.461219935079367 7 55 7.808038811705625 7 56 6.444885155090333 8 1 2.167147549152162 8 2 3.140993549302223 8 3 3.6371885357490186 8 4 3.4252514187182044 8 5 3.323301626502726 8 6 3.3986429168624284 8 7 3.29300441304882 8 8 3.5263442608329925 8 9 3.411529902276122 8 10 3.245433343896707 8 11 4.853942369242397 8 12 8.448055905861949 8 13 8.707996388285348 8 14 8.465382113504152 8 15 7.62251759407776 8 16 8.146647107964188 8 17 8.019963370504108 8 18 7.454125799249127 8 19 6.340853073164869 8 20 6.456798137433797 8 21 4.341456767726562 8 22 4.4278679477016 8 23 4.471796754236671 8 24 4.719028632466028 8 25 4.405828578067222 8 26 3.1014593783513362 8 27 3.261966705891041 8 28 2.960542800463429 8 29 3.183864656745392 8 30 2.9888359074544866 8 31 3.1637370287475193 8 32 4.307312934604283 8 33 2.965155572700205 8 34 2.846428330774806 8 35 2.954365841347382 8 36 -0.8091070185605638 8 37 -0.7900198032423766 8 38 -0.06413479211766315 8 39 1.2122609009228142 8 40 0.09442882669861419 8 41 -11.281969993398672 8 42 3.3477546036913566 8 43 6.28636283421551 8 44 4.64433353793509 8 45 3.299401065740369 8 46 5.545039313202317 8 47 5.794328629292014 8 48 5.985165323152923 8 49 7.5236653646858045 8 50 5.866015760228326 8 51 8.219178881712441 8 52 8.468205282811624 8 53 8.840324537625705 8 54 8.953844710884923 8 55 8.444977810200687 8 56 7.215402812335679 9 1 2.3768026202163384 9 2 3.3706137705773926 9 3 3.5043342120658636 9 4 3.5625039537254466 9 5 3.2916638963576617 9 6 3.5258289013689676 9 7 3.5070944346724815 9 8 3.630253315479983 9 9 3.5771556239396745 9 10 3.3656703644496844 9 11 5.3724589438860235 9 12 8.76493705968388 9 13 8.350261204694146 9 14 8.799089837141121 9 15 8.00572356472836 9 16 8.445111407924298 9 17 7.804660954112116 9 18 7.712943215746094 9 19 6.3131149504348265 9 20 6.388226978783276 9 21 4.688416858419991 9 22 4.781120944573516 9 23 4.481553645467535 9 24 5.008197905868926 9 25 4.444976857621313 9 26 3.554847785548027 9 27 3.547976405049191 9 28 3.3789397101688863 9 29 3.444099158036515 9 30 3.8583748825347897 9 31 4.151193204088145 9 32 5.0169942479794685 9 33 3.7293631598771597 9 34 3.6575046854028983 9 35 3.491099828289208 9 36 3.290954913293401 9 37 3.252303795943064 9 38 3.641022346840625 9 39 4.945444828032173 9 40 4.274283129387928 9 41 2.4612017572783813 9 42 7.851543993400174 9 43 10.690720107534428 9 44 9.421945697572315 9 45 7.559346901610461 9 46 9.511622354371255 9 47 9.637871971092867 9 48 9.738902228239734 9 49 11.558735589110047 9 50 9.740278351289145 9 51 9.53652714867022 9 52 9.416119543933114 9 53 9.313405174998955 9 54 9.503558144848771 9 55 8.95441454523732 9 56 8.198728447924452 10 1 1.8928022656535575 10 2 3.0193306965180895 10 3 3.3322050625733297 10 4 3.1870108898598204 10 5 3.054550037385006 10 6 3.278887005344508 10 7 3.111590733923471 10 8 3.2002573043112577 10 9 3.201130715904398 10 10 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2.1771485404373294 115 39 3.6257013343044058 115 40 4.906524282729997 115 41 5.7563712012027395 115 42 1.1445602824846155 115 43 4.415163095738883 115 44 3.2258400993697336 115 45 2.7670982590341637 115 46 0.4740170370149528 115 47 -0.07628268159389506 115 48 -5.003308968165435 115 49 -2.0853198312232237 115 50 1.5120158536295314 115 51 1.5410487738340493 115 52 1.3612609032291267 115 53 -0.41467009180501435 115 54 -0.16202882388367712 115 55 0.3223980689220234 115 56 1.6242012058349538 116 1 1.3867500483165358 116 2 2.2850040396218176 116 3 2.8013740361389807 116 4 2.571910200504128 116 5 2.4282133929731273 116 6 2.278887005344508 116 7 2.0995677192135958 116 8 2.180797932873382 116 9 1.7792396015364886 116 10 2.140444442723407 116 11 -0.3840781828706703 116 12 -0.13160281593031797 116 13 -7.840062816884387 116 14 -9.986241571991954 116 15 -3.0021020889843157 116 16 -9.972551719588328 116 17 -11.628782333521464 116 18 -3.9895187297578047 116 19 -3.909894704927165 116 20 -3.7847534699677077 116 21 2.2990669632201843 116 22 2.469486405178948 116 23 2.551125510910964 116 24 2.1543340430369113 116 25 2.2248191419218983 116 26 2.2817917432156207 116 27 2.5430112811787993 116 28 2.37095084304179 116 29 2.377052805426814 116 30 2.1245244203831177 116 31 0.5654288498978488 116 32 -6.286024341169419 116 33 0.5946176010000741 116 34 1.3070253533752734 116 35 0.8934037144865101 116 36 3.391606048915161 116 37 3.2213104275617965 116 38 4.041659738174847 116 39 5.607739093228204 116 40 7.513158846667931 116 41 1.740090531631541 116 42 -0.5403743655318962 116 43 -12.578951403993592 116 44 -3.877714131311304 116 45 -2.284174384854854 116 46 -7.327114201997507 116 47 -9.48120509999946 116 48 -9.033638403310277 116 49 -7.549724019864973 116 50 -3.780638736296325 116 51 -0.9911074594457823 116 52 -1.1407646260837225 116 53 -2.7486760537403505 116 54 -3.9197143700122403 116 55 -2.602022935434775 116 56 -0.8141997725339216 117 1 0.3146794223133256 117 2 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118 23 0.6261691409284111 118 24 -2.4425442047031503 118 25 0.6984352369863132 118 26 1.5288258824787135 118 27 1.5669734750333362 118 28 1.4557205353766278 118 29 1.4022503660676406 118 30 1.5609422488828315 118 31 -0.9378360480373189 118 32 -9.752483152472045 118 33 -0.5801377767672484 118 34 -0.28024448602704766 118 35 -0.5852126747128565 118 36 2.029859279497348 118 37 1.950608229393275 118 38 2.0580332503422305 118 39 3.9629168487824487 118 40 4.955439765153333 118 41 5.4857226276371875 118 42 1.2714969210143714 118 43 4.086068106958031 118 44 2.6368455486698714 118 45 2.3347443750722014 118 46 -1.0841195160355888 118 47 -3.7602019037330123 118 48 -6.905905399408088 118 49 -0.26433468323732967 118 50 -0.6737304867914524 118 51 -2.034717867901179 118 52 -2.2307895295094156 118 53 -3.789325503707996 118 54 -4.527495219866154 118 55 -5.454778979907978 118 56 -2.6768779692905937 119 1 0.7989873423725413 119 2 1.8814993531224289 119 3 2.4269586357004087 119 4 2.24635859079347 119 5 1.9878182909956834 119 6 1.5569526357417445 119 7 1.4575270058565906 119 8 1.4707010979584751 119 9 1.5355028217340347 119 10 1.184786683316446 119 11 -12.374724589887805 119 12 -14.256168611276808 119 13 -5.132935068538732 119 14 -5.05191078081771 119 15 -5.398290790930716 119 16 -6.703994928530516 119 17 -12.68331261626389 119 18 -2.846226342651015 119 19 -2.244081893221587 119 20 -1.874155965552891 119 21 -0.29583707512566804 119 22 0.4613747830485835 119 23 -0.1241767343394713 119 24 -6.524577680003588 119 25 -0.6110078360137123 119 26 1.4914713535472615 119 27 1.4684736242344343 119 28 1.3944124486772296 119 29 1.2915702803448283 119 30 1.6055940677798333 119 31 -1.4552383213194737 119 32 -9.279542805782953 119 33 -0.5505959597691756 119 34 -1.1921887119446346 119 35 -0.3621706000038003 119 36 2.3618181542498107 119 37 2.399377998538487 119 38 2.622067647976717 119 39 3.911944328911577 119 40 4.905932128769969 119 41 4.575975726950862 119 42 0.9183786181090206 119 43 1.5720838951467293 119 44 0.9733075837728222 119 45 1.9319285434795468 119 46 -2.0043376209103947 119 47 -2.959480312978055 119 48 -8.436871408685683 119 49 -0.7928592200357443 119 50 -3.2192096935841725 119 51 -8.673162921206663 119 52 -8.910495467713034 119 53 -12.200035429649997 119 54 -10.928466383363428 119 55 -11.788609650227812 119 56 -10.120791920949438 120 1 0.7804723449869826 120 2 1.5907972568159252 120 3 1.7405727441759495 120 4 1.647366339046549 120 5 1.4568015110365593 120 6 1.124452754579977 120 7 0.9084705814614722 120 8 0.9513542329111786 120 9 0.9280730551323373 120 10 0.9551199057340867 120 11 -9.53579793684792 120 12 -4.845154683435386 120 13 -6.915793841177418 120 14 -6.086231263776305 120 15 -5.280973682978061 120 16 -7.736081252626157 120 17 -10.553137211025504 120 18 -4.137974215446565 120 19 -3.170830003693613 120 20 -4.531497791858921 120 21 -0.2955280957084483 120 22 -0.14089740405033524 120 23 -0.8143179141654926 120 24 -5.478040626241171 120 25 -1.1444726836630927 120 26 0.7773248497682061 120 27 0.8828387903702932 120 28 0.2734351230129235 120 29 0.8913468644585336 120 30 0.08085194675496865 120 31 -4.665320557379747 120 32 -11.315574559359984 120 33 -5.1376093171870565 120 34 -8.012220085239303 120 35 -4.07082677721538 120 36 -2.2847331601010943 120 37 -2.0596228095615245 120 38 -2.7465923320584587 120 39 -0.9834542545366354 120 40 -1.8495700816212484 120 41 -1.681000077584006 120 42 -5.3778138047649655 120 43 -1.8574450300802496 120 44 -5.027595586831353 120 45 -12.165423740591375 120 46 -12.197093241959022 120 47 -12.137357386705297 120 48 -11.075317955246504 120 49 -14.964200190706467 120 50 -10.395042749628285 120 51 -10.351087530228861 120 52 -10.093054767993491 120 53 -10.693475558946 120 54 -10.23038680927967 120 55 -10.252007473111302 120 56 -7.844849533726926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 9301.0 5 91.0 6 115.0 7 549.0 8 1066.0 9 1137.0 10 665.0 11 783.0 12 716.0 13 812.0 14 1140.0 15 1603.0 16 2147.0 17 2652.0 18 3063.0 19 3747.0 20 4554.0 21 5438.0 22 6548.0 23 7460.0 24 8197.0 25 8546.0 26 9096.0 27 9875.0 28 10882.0 29 11458.0 30 12532.0 31 14522.0 32 16877.0 33 20131.0 34 23411.0 35 25964.0 36 28102.0 37 25331.0 38 15546.0 39 7903.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.37574513180553 0.42720890184130345 0.1334613856139886 0.06358458073917075 8 99.00615975625911 0.7189693999205193 0.14174062789773478 0.13313021592263877 9 96.41947376963923 2.41219280317209 0.5654508534763468 0.6028825737123322 10 47.73413697178434 34.903298450125845 5.86865810041065 11.493906477679163 11 31.823164532228525 43.7409133209473 16.277367669802917 8.158554477021259 12 54.01708835607365 14.591667770565639 19.321764472115515 12.069479401245198 13 28.40409325738508 16.459464829778778 26.50351039872831 28.632931514107828 14 34.774500223595076 19.815492654487635 13.958626629345611 31.451380492571673 15 26.29410437759584 22.579981688141082 15.178938708350472 35.94697522591261 16 29.839887891708162 29.074609658407624 22.000589694915703 19.08491275496851 17 40.29148241996655 21.34247528195956 20.523674665871713 17.842367632202183 18 29.21451021563958 23.699820162350672 29.480126780574885 17.60554284143486 19 34.11389847574507 20.077968190038355 24.514272087122993 21.29386124709358 20 14.777443942771773 27.20328343475366 30.35620231947237 27.663070303002197 21 18.358769524263742 28.434548176410583 39.41185432481486 13.794827974510818 22 21.233416567867057 34.07758694917836 26.980904629120605 17.708091853833977 23 25.28579944363492 23.877997085706713 34.95959729765532 15.876606173003047 24 29.995363624321104 25.528215657703008 23.901841303483906 20.574579414491986 25 18.455877969783877 17.54151239576365 36.36068592320786 27.64192371124461 26 29.494887159171597 16.801372271879707 41.71991851306247 11.983822055886236 27 33.39914401939869 17.033152685208496 33.46108998396693 16.106613311425882 28 49.26966012573192 20.079619155041716 22.187204538291837 8.46351618093453 29 52.53908837948854 20.08977076984232 19.570027825626077 7.801113025043064 30 50.13631560291911 26.69433834758326 15.219314082412142 7.950031967085497 31 44.813919832176005 29.395422016277866 15.831419201508293 9.95923895003784 32 33.1639784055907 34.08704004239394 19.133574007220215 13.615407544795152 33 27.78111387214391 44.25098783413012 18.07462750939728 9.893270784328688 34 18.790126648569277 50.919619093075596 18.664174132667775 11.626080125687354 35 15.739863364985125 58.68082935201077 18.163776480488757 7.415530802515356 36 14.355466806424436 59.108579844364684 17.317247921877176 9.2187054273337 37 12.182483302572003 60.200037179163736 19.95624809124829 7.6612314270159745 38 13.119608942067579 60.444185036197396 19.224914688218185 7.211291333516836 39 10.284139453396408 51.83857873757908 18.27541613614226 19.60186567288225 40 8.089754355098831 32.51562770735868 15.447772135364602 43.94684580217788 41 8.402603303576202 38.54472924573689 16.637488640615814 36.4151788100711 42 11.307911854506838 60.31959378733573 21.00657108721625 7.365923270941193 43 8.549286507108343 30.25947612939245 15.322982646204563 45.86825471729464 44 7.903436841421879 35.07689029607508 14.973215742285168 42.046457120217866 45 11.79902862731447 59.810698360601386 20.81555522402904 7.574717788055099 46 11.618921381824206 58.55886007787259 21.13594565487532 8.686272885427886 47 12.265376027773913 52.3228849887036 17.95697428660399 17.454764696918502 48 13.033841872484222 60.30810515330715 18.80534711524127 7.85270585896735 49 9.478742282155746 35.32130822860105 13.848255052737219 41.35169443650599 50 12.532692537471954 61.641530475419195 18.924498493156143 6.90127849395271 51 12.908938669998204 61.49148397472106 18.672456987925386 6.9271203673553465 52 13.053336391955822 60.877891146285265 18.80986339261077 7.258909069148141 53 11.740404365528386 60.67075699908083 18.67029907652284 8.918539558867936 54 10.545827129873102 51.350060846411715 17.201794554660633 20.902317469054545 55 12.259682362775147 60.947471772214044 18.98426408735687 7.808581777653943 56 12.521443669098883 60.66078023432912 19.635598647514193 7.182177449057805 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 2.0 5 4.0 6 13.0 7 22.0 8 46.5 9 71.0 10 290.0 11 509.0 12 861.0 13 1477.5 14 1742.0 15 1714.5 16 1687.0 17 1774.0 18 1861.0 19 1951.0 20 2041.0 21 2090.0 22 2461.5 23 2784.0 24 3283.0 25 3782.0 26 4355.0 27 4928.0 28 5958.0 29 8447.5 30 9907.0 31 12189.0 32 14471.0 33 17178.0 34 19885.0 35 22280.0 36 24675.0 37 25018.0 38 23973.5 39 22586.0 40 21018.5 41 19451.0 42 17739.0 43 16027.0 44 14428.5 45 12830.0 46 11820.0 47 10333.5 48 9857.0 49 9693.5 50 9530.0 51 10045.0 52 10560.0 53 11424.5 54 12508.0 55 12727.0 56 12218.5 57 11710.0 58 10396.0 59 9082.0 60 7767.5 61 6453.0 62 5392.5 63 3701.5 64 3071.0 65 2560.0 66 2049.0 67 1914.5 68 1780.0 69 1600.5 70 1421.0 71 1043.0 72 501.5 73 338.0 74 254.0 75 170.0 76 126.5 77 83.0 78 60.0 79 26.0 80 15.0 81 11.5 82 8.0 83 6.5 84 5.0 85 3.0 86 1.0 87 1.0 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02550006623393827 10 0.0 11 0.0023181878394489337 12 0.0 13 0.0 14 0.024837726851238577 15 0.1708835607365214 16 0.0360974963571334 17 0.018214333024241622 18 0.006954563518346801 19 0.013909127036693602 20 0.02682474499933766 21 0.009272751357795735 22 6.623393826996952E-4 23 0.0 24 0.0 25 6.623393826996952E-4 26 0.18611736653861438 27 0.027818254073387203 28 0.17386408795867003 29 0.026493575307987814 30 0.028811763147436747 31 0.2291694264140946 32 0.00993509074049543 33 0.17982514240296726 34 0.08577295005961055 35 0.044376738640879584 36 0.16094846999602597 37 0.23711749900649093 38 0.13876010067558617 39 0.38316333289177373 40 0.6133262683799179 41 0.8769373426943967 42 0.2119486024639025 43 0.34640349715194063 44 0.5288779970857067 45 0.10763014968870048 46 0.06126639289972182 47 0.031129950986885677 48 0.03808451450523248 49 0.3424294608557425 50 0.22122135382169825 51 0.330507351967148 52 0.38779970857067164 53 0.19837064511855876 54 0.12617565240429196 55 0.16028613061332628 56 0.19505894820506028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 301960.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.69400882600736 #Duplication Level Percentage of deduplicated Percentage of total 1 93.96908794351472 71.12896972168267 2 3.487880954532742 5.2802338351292875 3 0.9208642840655326 2.0911172773683413 4 0.41663038472615027 1.2614569607457624 5 0.239193520640305 0.9052758231235511 6 0.15823119613157338 0.718629213391981 7 0.10955623226127484 0.5804925288210335 8 0.09107316958961857 0.5514954642183243 9 0.07443828518515531 0.50710789942182 >10 0.43536689852104765 6.359817610248244 >50 0.05043112733167063 2.5865452871931973 >100 0.04459173364063509 6.632919536383114 >500 0.0026542698595616123 1.3959388422726684 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAACCAAAAAAAAAAAAAAA 914 0.30268909789376075 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAACCAAAAAAAAAAAAAAA 698 0.23115644456219367 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAACCAAAAAAAAAAAAAAA 657 0.2175784872168499 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAACCAAAAAAAAAAAAAAA 652 0.21592263876010065 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAACCAAAAAAAAAAAAAAA 553 0.18313683931646577 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAACCAAAAAAAAAAAAAAA 458 0.15167571863823023 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAACCAAAAAAAAAAAAAAA 458 0.15167571863823023 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAACCAAAAAAAAAAAAAAA 401 0.1327990462312889 No Hit ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 401 0.1327990462312889 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAACCAAAAAAAAAAAAAAA 394 0.13048085839183998 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAACCAAAAAAAAAAAAAAA 394 0.13048085839183998 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 391 0.12948734931779043 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAACCAAAAAAAAAAAAAAA 374 0.12385746456484302 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAACCAAAAAAAAAAAAAAA 372 0.12319512518214333 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAACAAAAAAAAAAAAAAA 350 0.1159093919724467 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAACCAAAAAAAAAAAAAAA 310 0.10266260431845277 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAACAAAAAAAAAAAAAAA 304 0.10067558617035369 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAACCAAAAAAAAAAAAAAA 303 0.10034441647900384 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.311696913498477E-4 0.0 0.0 0.0 0.0 8 3.311696913498477E-4 0.0 0.0 0.0 0.0 9 6.623393826996954E-4 0.0 0.0 0.0 0.0 10 0.03179229036958538 0.0 0.0 0.0 0.0 11 0.0625910716651212 0.0 0.0 0.0 0.0 12 0.06888329580076831 0.0 0.0 0.0 0.0 13 0.07385084117101604 0.0 0.0 0.0 0.0 14 0.08842230759040932 0.0 0.0 0.0 0.0 15 0.09769505894820506 0.0 0.0 0.0 0.0 16 0.11690290104649623 0.0 0.0 0.0 0.0 17 0.1271691614783415 0.0 0.0 0.0 0.0 18 0.14008477944098557 0.0 0.0 0.0 0.0 19 0.15068220956418069 0.0 0.0 0.0 0.0 20 0.17750695456351834 0.0 0.0 0.0 0.0 21 0.19704596635315935 0.0 0.0 0.0 0.0 22 0.21857199629089946 0.0 0.0 0.0 0.0 23 0.23645515962379124 0.0 0.0 0.0 0.0 24 0.2550006623393827 0.0 0.0 0.0 0.0 25 0.2689097893760763 0.0 0.0 0.0 0.0 26 0.2824877467214201 0.0 0.0 0.0 0.0 27 0.29904623128891245 0.0 0.0 0.0 0.0 28 0.31825407338720363 0.0 0.0 0.0 0.0 29 0.3248774672142006 0.0 0.0 0.0 0.0 30 0.32818916412769905 0.0 0.0 0.0 0.0 31 0.3344813882633461 0.0 0.0 0.0 0.0 32 0.3381242548681945 0.0 0.0 0.0 0.0 33 0.3384554245595443 0.0 0.0 0.0 0.0 34 0.33944893363359385 0.0 0.0 0.0 0.0 35 0.3397801033249437 0.0 0.0 0.0 0.0 36 0.34077361239899323 0.0 0.0 0.0 0.0 37 0.34507881838654125 0.0 0.0 0.0 0.0 38 0.3467346668432905 0.0 0.0 0.0 0.0 39 0.34772817591734007 0.0 0.0 0.0 0.0 40 0.35070870313948865 0.0 0.0 0.0 0.0 41 0.35534507881838656 0.0 0.0 0.0 0.0 42 0.3709100543118294 0.0 0.0 0.0 0.0 43 0.3911114054841701 0.0 0.0 0.0 0.0 44 0.4172738111008081 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCCA 15 0.00810362 50.494286 39 ACTGCTT 30 9.766864E-7 50.494286 40 TCGGCGA 20 3.9808798E-4 50.494286 39 CCGTCCA 15 0.00810362 50.494286 39 AAGCCCG 15 0.00810362 50.494286 40 CCCGAAT 20 3.9808798E-4 50.494286 39 ACCTCCC 20 3.9841823E-4 50.485806 38 TGAAACC 15 0.008109004 50.4858 38 ACCGTCC 15 0.008109004 50.4858 38 ACCCGAA 15 0.008109004 50.4858 38 AACCGAT 20 4.0040415E-4 50.434975 41 CACCATC 15 0.008141365 50.43497 41 AACGAAC 20 4.0239797E-4 50.384243 37 AACCCAT 15 0.0081738215 50.38424 37 CTTGAAC 30 9.915002E-7 50.38424 37 ACGCGCA 15 0.008217246 50.316757 36 GATACCC 40 2.5520421E-9 50.25786 35 ACTTTCC 30 1.0088297E-6 50.25786 35 GCCCGGA 15 0.008321068 50.157207 42 TGCGGCA 15 0.008332054 50.140472 47 >>END_MODULE