FastQCFastQC Report
Fri 17 Jun 2016
SRR1526336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48149
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1690.3509937901098673No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1360.28245654115350266No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC860.1786122245529502No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC760.15784336123283974No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC710.14745892957278448No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC610.12669006625267398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACTA150.00790242750.74973339
AGCACCG150.00790242750.74973339
GCACCGA150.00790242750.74973340
CTGCACT150.00790242750.74973338
GATACCC150.00813722650.3757935
TAAGATC203.9929216E-450.3757935
ATACCCA150.00813722650.3757936
TCGACCC150.00820525550.2699643
ACCATGT150.00820525550.2699642
TCGCCTA150.00820525550.2699642
CCTGATT150.00823942750.2172149
TCCGACG150.00823942750.2172149
CTCAAAG150.00823942750.2172144
CAATAGC150.00827370550.1645745
TCAAAGA150.00827370550.1645745
ATAGCAG150.00827370550.1645747
AATAGCA150.00827370550.1645746
ATGTTCC150.00827370550.1645745
CGCGCCG150.00827370550.1645747
CTACCAA252.0176443E-550.16456646