##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526336_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48149 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.46472408565079 38.0 38.0 39.0 35.0 40.0 2 36.872686867847726 38.0 37.0 39.0 33.0 40.0 3 37.04882759766558 38.0 38.0 39.0 34.0 40.0 4 37.062410434276934 38.0 38.0 39.0 35.0 40.0 5 36.924962096824444 38.0 37.0 39.0 34.0 40.0 6 35.409229682859454 37.0 35.0 39.0 30.0 39.0 7 37.162744812976385 38.0 38.0 39.0 35.0 40.0 8 36.880163658642964 38.0 37.0 39.0 33.0 40.0 9 35.72022264221479 38.0 35.0 39.0 31.0 40.0 10 35.51220170720057 37.0 35.0 39.0 31.0 40.0 11 32.92988431743131 35.0 30.0 38.0 24.0 39.0 12 31.44551288707969 35.0 28.0 38.0 16.0 39.0 13 31.202351035327837 35.0 28.0 38.0 15.0 39.0 14 30.875179131446135 35.0 27.0 38.0 13.0 39.0 15 31.5337182496002 35.0 28.0 38.0 15.0 39.0 16 32.416602629338094 36.0 30.0 38.0 18.0 40.0 17 32.366383517830066 36.0 30.0 38.0 18.0 39.0 18 32.61147687387069 36.0 30.0 38.0 20.0 39.0 19 33.34231240524206 36.0 31.0 38.0 24.0 39.0 20 33.48916903777856 36.0 31.0 38.0 24.0 40.0 21 34.80954952335458 38.0 34.0 39.0 27.0 40.0 22 34.423394047643775 38.0 33.0 39.0 25.0 40.0 23 34.69650460030323 38.0 33.0 39.0 27.0 40.0 24 34.34063012731313 38.0 33.0 39.0 25.0 40.0 25 34.554923259050035 38.0 33.0 39.0 26.0 40.0 26 34.767014891275004 38.0 35.0 39.0 27.0 40.0 27 34.66447901306361 38.0 35.0 39.0 27.0 40.0 28 34.568879935201146 38.0 34.0 39.0 27.0 40.0 29 34.423747118320215 38.0 34.0 39.0 26.0 40.0 30 34.223493738187706 38.0 33.0 39.0 25.0 39.0 31 33.865708529772164 38.0 33.0 39.0 24.0 39.0 32 32.80290348709215 37.0 32.0 39.0 18.0 39.0 33 34.13886062015826 38.0 33.0 39.0 25.0 40.0 34 33.69149930424308 37.0 33.0 39.0 23.0 40.0 35 33.714677355708325 37.0 33.0 39.0 24.0 40.0 36 32.0040291594841 35.0 31.0 38.0 21.0 39.0 37 31.874659909863134 35.0 31.0 38.0 20.0 39.0 38 31.104508920226795 35.0 30.0 38.0 15.0 39.0 39 29.003343786994538 33.0 26.0 38.0 6.0 39.0 40 25.27090905314752 31.0 12.0 37.0 4.0 39.0 41 22.37001806891109 26.0 9.0 33.0 4.0 37.0 42 25.507632557270142 30.0 20.0 34.0 4.0 37.0 43 22.014849737273877 26.0 9.0 33.0 4.0 37.0 44 22.78786683004839 27.0 10.0 33.0 4.0 37.0 45 25.14087519990031 30.0 19.0 34.0 4.0 37.0 46 24.861388606201583 30.0 15.0 35.0 4.0 38.0 47 24.31589441109888 29.0 10.0 35.0 4.0 38.0 48 24.285177262248435 29.0 10.0 35.0 4.0 38.0 49 21.725747159857942 26.0 5.0 35.0 4.0 38.0 50 24.027601819352427 29.0 4.0 35.0 4.0 38.0 51 25.512741697646888 32.0 4.0 37.0 4.0 39.0 52 25.267876799102787 32.0 4.0 37.0 4.0 39.0 53 24.98706099815157 31.0 4.0 37.0 4.0 39.0 54 24.205819435502296 31.0 4.0 37.0 4.0 39.0 55 24.20521713846601 31.0 4.0 36.0 4.0 39.0 56 23.775488587509606 31.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.0547938499633176 1 2 0.17370679040471515 1 3 0.3378191767123653 1 4 0.4990345611122535 1 5 0.7512232407056914 1 6 0.8656282775022319 1 7 1.0632432071269733 1 8 1.3540785461028193 1 9 1.4244598959524595 1 10 0.8775581770194094 1 11 -6.636993250828766 1 12 -9.733494449098195 1 13 -21.57708901430266 1 14 -19.176685873077915 1 15 -13.067761691133384 1 16 -21.023352928996495 1 17 -21.175391559980824 1 18 -20.861072091318977 1 19 -6.513973376464879 1 20 -11.470262647731921 1 21 -6.951167394922106 1 22 -2.7721984332423872 1 23 -3.853377002083235 1 24 -3.3720653012861277 1 25 -2.9526866093461663 1 26 2.6602807554196204 1 27 2.989738888677735 1 28 2.9940058116856534 1 29 2.235797368567404 1 30 2.6626117477910327 1 31 0.6235464384377991 1 32 -11.897578167177286 1 33 -0.10911727058240217 1 34 0.14145094555825466 1 35 -0.23372420625758394 1 36 3.234485874927639 1 37 3.374289903391748 1 38 3.967247160831114 1 39 7.328428336876481 1 40 7.8947345309858115 1 41 -4.107669109173273 1 42 -9.081027573240815 1 43 -13.141103702448099 1 44 -15.626107024118326 1 45 -16.012133296624977 1 46 -18.484488963290765 1 47 -18.093558658100754 1 48 -17.691939736570482 1 49 -14.89947516212204 1 50 -17.67567068061217 1 51 -18.981852564946948 1 52 -18.39919833206904 1 53 -18.228203258448907 1 54 -17.700402954034722 1 55 -17.424261141135315 1 56 -16.9328581664577 2 1 0.20532175343239345 2 2 0.7136766244921944 2 3 0.4998101269386055 2 4 0.4649470799659454 2 5 0.4106500883678379 2 6 1.1328982624192818 2 7 0.7238766912898669 2 8 1.08198201518276 2 9 1.4437660799645258 2 10 0.9170755224191112 2 11 -5.456299434840833 2 12 -12.766978611994123 2 13 -14.87633486648969 2 14 -16.493126295400693 2 15 -11.765197588569283 2 16 -4.980818992344911 2 17 -4.337080851081879 2 18 -11.404058516658342 2 19 -3.4838074639460253 2 20 -5.0041992993156335 2 21 1.5260573411261618 2 22 1.649521023771186 2 23 0.9888552754431572 2 24 1.449352496602259 2 25 1.354100720970571 2 26 1.9169926709550609 2 27 2.1203572898843674 2 28 1.7342773048983275 2 29 1.8864761015990794 2 30 2.209519741757852 2 31 -0.8666496399935752 2 32 -8.344938649831889 2 33 -1.5064023384557075 2 34 -1.259755690942498 2 35 -1.4038599528639217 2 36 2.511107292725523 2 37 2.5293426937386556 2 38 3.311440222671237 2 39 5.610177959802574 2 40 8.215699840186414 2 41 3.7695556268749932 2 42 -1.2973171660009974 2 43 -2.2762469905325666 2 44 -10.54073749168997 2 45 -1.920127263442474 2 46 -11.928229536443101 2 47 -12.640466652067571 2 48 -10.113357534458867 2 49 -10.919686323509671 2 50 -10.196334330687584 2 51 -9.6409777534839 2 52 -9.339771484406901 2 53 -9.051129351963235 2 54 -14.2430877202489 2 55 -8.458348622281617 2 56 -9.15457762347127 3 1 0.31044995856060353 3 2 0.2521381629537345 3 3 0.14852807565655723 3 4 0.5880240030428681 3 5 0.5106500883678322 3 6 0.9893085188295387 3 7 1.3905433579565312 3 8 1.805058938259684 3 9 2.574535310733758 3 10 1.4786139839575725 3 11 0.007803129261731101 3 12 0.3637906187751092 3 13 -6.589155379310203 3 14 -7.200818603093001 3 15 -3.016479639851333 3 16 2.8114886999627764 3 17 2.8065088925078676 3 18 1.5061978935980669 3 19 0.6956797155411572 3 20 0.5214417263253921 3 21 3.341441956510778 3 22 3.308495382745548 3 23 3.0350091215970068 3 24 3.198070445320205 3 25 2.4387161055859607 3 26 2.6708388248012156 3 27 2.7280495975766783 3 28 2.659918330539348 3 29 2.8172453323683087 3 30 2.4069556391937468 3 31 2.3641195907756583 3 32 1.3165998117065776 3 33 3.2166745846212166 3 34 3.3940904629036552 3 35 2.2474220984181343 3 36 4.5829021645203945 3 37 4.68575295014891 3 38 6.052465863696881 3 39 8.548639498264112 3 40 9.59005881454539 3 41 9.14647870379807 3 42 8.336016167332335 3 43 4.116060701775126 3 44 4.066954816002337 3 45 8.015770172454964 3 46 -0.9128449210584861 3 47 -5.453287164888085 3 48 -1.108229329330662 3 49 -9.414558118381466 3 50 -1.4117189460721988 3 51 -3.1409777534838987 3 52 -1.9859253305607467 3 53 -3.8767703776042595 3 54 -5.361036438197619 3 55 -3.2455281094611053 3 56 -0.4494494183430646 4 1 0.43834239133108355 4 2 0.3131137727098334 4 3 0.8198851738429198 4 4 0.49233920004099474 4 5 0.7414193191370657 4 6 1.9392772492860715 4 7 1.8430136518902458 4 8 1.7806686943572458 4 9 2.6552107328725967 4 10 2.0470942841451887 4 11 4.339572985421832 4 12 5.2277681047038165 4 13 5.498712037825509 4 14 3.885485336869479 4 15 5.9241082275657995 4 16 6.400606898837079 4 17 5.936277497886227 4 18 5.3792435471315265 4 19 4.286361391588688 4 20 4.350397323573674 4 21 2.7157383917828213 4 22 3.48892064853667 4 23 3.354896551240536 4 24 3.2099528718367765 4 25 2.4612301768805196 4 26 2.4775930461895825 4 27 2.052627458739906 4 28 2.4100746782566773 4 29 2.678408559385197 4 30 2.567681092602129 4 31 2.4382284087869124 4 32 3.312847466490812 4 33 2.624742126835095 4 34 2.7909009694702647 4 35 2.3753145311886144 4 36 4.933433746759295 4 37 4.041913050211448 4 38 5.065286376517392 4 39 8.474530680252858 4 40 9.5190769508806 4 41 4.766553750702386 4 42 7.9304501885956284 4 43 7.194859951306086 4 44 11.388718418253745 4 45 9.21120481910912 4 46 9.16783050108035 4 47 9.50137199708815 4 48 9.640676236648073 4 49 5.405642006696709 4 50 9.379087808149187 4 51 7.146076655521728 4 52 6.932461160996475 4 53 6.245493537342583 4 54 1.8287696906328996 4 55 6.1787995953544055 4 56 5.732852020055933 5 1 0.49474483035547223 5 2 0.3771381629537345 5 3 0.32384858847706965 5 4 0.8014855415044053 5 5 0.8289193191370643 5 6 0.5261674931885096 5 7 1.3286843835975617 5 8 1.7050589382596826 5 9 2.353381464579911 5 10 1.0074601378037258 5 11 4.684085180543782 5 12 6.687829080313566 5 13 6.56212667197185 5 14 5.005912166137769 5 15 6.9155716421999465 5 16 6.088411776885859 5 17 4.979265302764279 5 18 4.691133791033966 5 19 4.204654074515513 5 20 3.727531469915135 5 21 2.227018879587696 5 22 2.882213331463497 5 23 2.805201429289312 5 24 2.4541601889099525 5 25 2.1752545671244192 5 26 0.7343003632627543 5 27 0.7068957514228345 5 28 1.5506234587444823 5 29 2.283591486214462 5 30 1.0018274340655395 5 31 2.0333503600064233 5 32 3.6021767347834945 5 33 1.7445592000058312 5 34 2.291205847519045 5 35 2.044216970213 5 36 3.1937996004178295 5 37 3.1245350014309636 5 38 3.2902863765173933 5 39 6.391908729033343 5 40 4.347430609417184 5 41 -1.104482834663468 5 42 6.734413603229772 5 43 8.993945317159742 5 44 9.525608662156184 5 45 7.19718042886522 5 46 8.616001232787667 5 47 9.029725655624738 5 48 9.69433477323344 5 49 8.612044445721097 5 50 8.37481951546626 5 51 8.318637631131484 5 52 7.9284977463623285 5 53 8.329639878805995 5 54 8.029989202828023 5 55 9.003189839256844 5 56 7.437730068836419 6 1 0.7227853708960126 6 2 0.6440300548456293 6 3 0.8505377776662542 6 4 1.0048639198827871 6 5 1.1333112110289605 6 6 1.6528566823776956 6 7 2.105373572786746 6 8 2.35911299231374 6 9 3.1337868699853146 6 10 2.5919195972631854 6 11 5.464490585949186 6 12 7.852356107340594 6 13 7.218883428728606 6 14 7.460304058029664 6 15 7.96827434490265 6 16 7.1451685336426145 6 17 6.176224762223736 6 18 6.106336493736666 6 19 4.676275696137136 6 20 4.602869307752975 6 21 3.737491852560673 6 22 4.068024142274311 6 23 3.058917645505531 6 24 4.238957486207248 6 25 3.6705248373946944 6 26 2.7228138767762715 6 27 3.5170308865579685 6 28 1.815150485771504 6 29 2.403523918646897 6 30 2.9585841908223003 6 31 3.56578279243886 6 32 3.4964334915402553 6 33 2.9266537946004263 6 34 2.792219361032558 6 35 1.808406159402189 6 36 1.0593401409583727 6 37 1.5478458122417749 6 38 1.9166377278687428 6 39 4.144273593898209 6 40 0.9524981769847543 6 41 0.21612527344463928 6 42 6.339481170797342 6 43 9.434148019862445 6 44 8.967162716210236 6 45 6.001910158594949 6 46 8.281541773328208 6 47 9.421279709678789 6 48 9.318659097557763 6 49 11.014409310585961 6 50 8.46873843438518 6 51 9.000394387888242 6 52 9.212619367983951 6 53 8.730991230157347 6 54 9.198908121746943 6 55 9.027514163581166 6 56 7.524216555322909 7 1 0.6967375839786598 7 2 0.9332975832435864 7 3 0.9294645305060527 7 4 0.5612681502000569 7 5 1.0095352611660502 7 6 1.7317834352174941 7 7 1.8654597459164037 7 8 2.0804212571002623 7 9 3.373309000811794 7 10 2.436807963890679 7 11 5.356186629819142 7 12 7.426778355675889 7 13 7.954880295160255 7 14 7.626383180630519 7 15 7.120644105968065 7 16 6.8376871392047 7 17 6.158794288271526 7 18 5.526822196831066 7 19 4.967697552776382 7 20 4.489669151074558 7 21 3.713794241906541 7 22 4.264459708275091 7 23 3.2992231684197506 7 24 4.10108047876502 7 25 3.8350371758200694 7 26 2.383213406741014 7 27 1.9905914035967456 7 28 3.056239400773464 7 29 3.152250906504314 7 30 3.1221172891380036 7 31 3.3652344179774403 7 32 4.659966589855959 7 33 3.4407548521797438 7 34 3.988850775055276 7 35 2.800376390502855 7 36 1.641988006214934 7 37 1.4399335521556011 7 38 1.7754313040536225 7 39 4.025785540627549 7 40 -2.426663593481365 7 41 -10.70357703756202 7 42 3.1269860669978904 7 43 8.028546766435106 7 44 7.369630401286617 7 45 3.9750789795898562 7 46 6.791725870468827 7 47 7.577370583160967 7 48 7.400131874682714 7 49 9.63505169209791 7 50 7.441123863292347 7 51 8.58421734127641 7 52 9.482302094188416 7 53 9.321668864313242 7 54 10.12056891297295 7 55 9.942320274039453 7 56 9.044976445648015 8 1 -0.19275516964452777 8 2 0.057693718509291614 8 3 0.36968192181040394 8 4 0.25148554150440816 8 5 0.38030820802595144 8 6 0.35255638207739537 8 7 1.0828510502642246 8 8 1.2772811604819054 8 9 1.8256036868021326 8 10 1.17412680447039 8 11 4.892418513877118 8 12 6.325329080313569 8 13 7.017682227527406 8 14 7.179523277248883 8 15 6.751682753311062 8 16 5.555078443552521 8 17 5.244543080542055 8 18 4.959189346589522 8 19 3.4435429634044006 8 20 3.987253692137358 8 21 1.7228522129210333 8 22 2.132213331463497 8 23 1.801034762622649 8 24 1.927771300021064 8 25 1.011365678235535 8 26 1.6120781410405343 8 27 1.8402290847561673 8 28 0.9825679031889223 8 29 0.2905359306589048 8 30 0.03516076739887808 8 31 1.0361281377842033 8 32 2.2257878458946116 8 33 1.1098369777836083 8 34 0.5009280697412635 8 35 0.9108836368796673 8 36 -1.370089288471057 8 37 -1.2351872207912606 8 38 0.25973082096183475 8 39 1.8488531734777887 8 40 -2.740069390582814 8 41 -10.433649501330136 8 42 2.258024714340884 8 43 7.550889761604186 8 44 6.260330884378405 8 45 4.272180428865219 8 46 6.282667899454335 8 47 7.158892322291404 8 48 6.105445884344551 8 49 9.316211112387766 8 50 7.049819515466261 8 51 7.840859853353706 8 52 9.271553301917887 8 53 8.365750989917107 8 54 9.668878091716913 8 55 9.300412061479065 8 56 8.273841179947532 9 1 0.7547085984714172 9 2 0.3970656991856174 9 3 0.7845369942741698 9 4 0.8366304690406423 9 5 0.7776512031950347 9 6 1.311493580145033 9 7 2.1987930792497323 9 8 2.2833198078249026 9 9 2.8660626240002003 9 10 2.378836949397929 9 11 5.443143151558274 9 12 7.325329080313569 9 13 7.404155657479098 9 14 7.9017454994711045 9 15 7.714847004518791 9 16 6.055078443552521 9 17 6.303721824503409 9 18 6.0195758200194724 9 19 4.503929436834358 9 20 4.895466252523832 9 21 3.322489894080448 9 22 4.467358258999731 9 23 3.574585487260329 9 24 3.9851384497795124 9 25 3.7915589149505067 9 26 3.4266916676105836 9 27 3.0195769108431207 9 28 2.693920560193753 9 29 2.833410326794173 9 30 3.5568998978336595 9 31 3.944944562904972 9 32 4.12373470579799 9 33 3.3682910840637987 9 34 3.742473963461073 9 35 2.1047242165898155 9 36 1.960828585925082 9 37 2.483411813025164 9 38 3.804416811300001 9 39 4.663466700047838 9 40 0.03710452246066254 9 41 -3.1383596462576726 9 42 6.663217951055859 9 43 9.912604737449598 9 44 8.702963734619953 9 45 6.815658689734786 9 46 9.03810268206303 9 47 9.200558988958068 9 48 9.603030425407354 9 49 12.08432705441675 9 50 9.97735574735032 9 51 9.395811544174961 9 52 9.09099774636233 9 53 9.263697849820488 9 54 9.497380507175848 9 55 10.232175346503219 9 56 8.82758514130019 10 1 0.8006658829870545 10 2 0.738980268216892 10 3 0.9245064832139107 10 4 0.955432909925463 10 5 1.083524582294963 10 6 1.529456966872722 10 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114 32 -0.6577591626524004 114 33 2.447443815390443 114 34 2.688962257775451 114 35 2.1961400471360832 114 36 3.9931585747768032 114 37 3.442163206559165 114 38 5.3729786842096985 114 39 7.574280523905141 114 40 10.000315224801799 114 41 7.518273575592939 114 42 3.6052469365631055 114 43 1.8981119838264107 114 44 0.3361855852331068 114 45 2.7208983775831683 114 46 -3.8872038954174606 114 47 -6.645594857195777 114 48 -4.8518190729204065 114 49 -13.427378631201979 114 50 -2.6937702281234834 114 51 -2.1281572406633877 114 52 -1.4474637920992102 114 53 -2.2357447365786207 114 54 -3.1302672074283855 114 55 -1.7711691351021308 114 56 3.2043967355030887 115 1 0.3822448303554751 115 2 0.30213816295373164 115 3 0.6113485884770711 115 4 0.6014855415044096 115 5 0.8664193191370657 115 6 1.2886674931885125 115 7 1.3161843835975588 115 8 1.2300589382596812 115 9 1.553381464579914 115 10 0.8824601378037258 115 11 -2.565914819456218 115 12 -6.299670919686431 115 13 -2.7128733280281523 115 14 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116 35 -0.4334146087343669 116 36 1.5714311793652023 116 37 2.3949297382730634 116 38 2.565286376517392 116 39 5.121513992191243 116 40 6.963877977838237 116 41 2.686964533757582 116 42 0.5087557084929308 116 43 -10.636975735471836 116 44 -2.007943969422765 116 45 -0.6488722027137293 116 46 -7.127419819843912 116 47 -9.316984870691053 116 48 -9.874086279398139 116 49 -6.324139764805217 116 50 -6.134391010849528 116 51 -2.5984676320264093 116 52 -2.8300548852166187 116 53 -4.3572022264571615 116 54 -6.562116060329872 116 55 -3.7704943712694714 116 56 -1.514901510110949 117 1 0.08224483035547081 117 2 0.6521381629537331 117 3 0.6413485884770651 117 4 0.36648554150441015 117 5 0.561419319137066 117 6 1.2436674931885108 117 7 0.9761843835975625 117 8 1.1650589382596834 117 9 0.6683814645799089 117 10 -0.22753986219627365 117 11 -12.120914819456218 117 12 -12.314670919686431 117 13 -8.93787332802815 117 14 -10.31158783386223 117 15 -11.054428357800052 117 16 -11.611588223114143 117 17 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-0.11775516964452493 120 2 0.21880482962040304 120 3 0.2946819218104011 120 4 -0.04018112516225614 120 5 0.44141931913706856 120 6 -0.9363325068114889 120 7 0.3495177169308903 120 8 0.5050589382596868 120 9 -0.138285202086756 120 10 -1.3675398621962742 120 11 -10.07424815278955 120 12 -2.8080042530197638 120 13 -6.771206661361486 120 14 -6.031587833862233 120 15 -8.767761691133387 120 16 -11.578254889780808 120 17 -7.816568030569059 120 18 -2.7963662089660346 120 19 -1.9120125921511502 120 20 -3.134968530084862 120 21 -0.8354811204123038 120 22 -0.3761200018698361 120 23 -0.7572985707106881 120 24 -8.358339811090051 120 25 -0.8664120995422451 120 26 2.6093003632627543 120 27 2.1818957514228288 120 28 1.3881234587444808 120 29 2.6377581528811263 120 30 2.168494100732211 120 31 -2.299982973326909 120 32 -7.3936565985498355 120 33 -2.0346074666608374 120 34 -6.921294152480957 120 35 0.052550303546333055 120 36 0.25213293375116663 120 37 1.8703683347642936 120 38 1.0652863765173919 120 39 3.0460753957000115 120 40 -1.4817360572494813 120 41 -0.45864950133013593 120 42 -5.928086396770226 120 43 -3.0018880161735915 120 44 -5.186891337843816 120 45 -11.061152904468114 120 46 -13.792332100545666 120 47 -13.09944101104193 120 48 -11.30566522676656 120 49 -14.583788887612236 120 50 -10.383513817867073 120 51 -9.856362368868515 120 52 -10.975668920304336 120 53 -11.987026787860671 120 54 -12.620010797171977 120 55 -12.83014349407649 120 56 -9.96226993116358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 223.0 5 42.0 6 74.0 7 90.0 8 132.0 9 192.0 10 85.0 11 109.0 12 83.0 13 118.0 14 138.0 15 174.0 16 258.0 17 302.0 18 420.0 19 575.0 20 671.0 21 853.0 22 1019.0 23 1239.0 24 1360.0 25 1463.0 26 1518.0 27 1702.0 28 1726.0 29 1898.0 30 2150.0 31 2568.0 32 3054.0 33 3718.0 34 4207.0 35 4851.0 36 4994.0 37 3894.0 38 1821.0 39 428.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.14639971754346 0.7248333298718561 0.09553677127250826 0.03323018131217678 8 99.38939541838874 0.44653056138237557 0.13292072524870713 0.031153294980165735 9 98.22370881289733 1.1197905846179417 0.2638467611251922 0.39265384135953796 10 62.19651498473488 26.21238239631145 5.1652163077114785 6.425886311242186 11 42.12963924484413 23.686888616586014 18.253753972045107 15.929718166524745 12 39.00184842883549 12.205860973228935 22.255913933830403 26.53637666410517 13 29.352634530312155 10.895345697729963 25.00571143741303 34.74630833454485 14 22.05949561676846 12.676056338028168 31.262204495409033 34.00224354979434 15 28.046699408973613 14.463497877299591 28.148672271705657 29.341130442021147 16 36.5961602393617 16.179770611702125 23.81150265957447 23.4125664893617 17 33.030743664312425 19.858745326132116 24.216867469879517 22.893643539675946 18 32.91307508567868 23.026274794890437 21.991899470349985 22.0687506490809 19 31.589223770849344 25.926926032860436 20.686288764721766 21.79756143156845 20 31.68720004986184 26.738412315874765 20.526457939459412 21.04792969480398 21 29.603705396086905 24.859801437294895 24.064304407427407 21.472188759190793 22 27.246671789652954 21.724231032835572 29.155330328771107 21.87376684874037 23 31.780514652433073 20.8270161374068 25.508317929759706 21.884151280400424 24 34.754615879872894 19.890340401669818 22.30991297846269 23.045130739994597 25 30.44923051361399 19.919416810317973 21.097011360568235 28.534341315499802 26 24.99167776298269 20.62666444740346 26.539613848202396 27.842043941411454 27 23.294415821838125 18.688715306631213 26.31917899285358 31.69768987867708 28 23.629894719320877 25.87907286421705 23.50713661520536 26.983895801256708 29 29.743824146599906 24.48525898069851 21.6845691965677 24.086347676133883 30 28.286794648051195 21.20626610155406 27.659353444693757 22.84758580570099 31 31.800774258002747 20.503267701785788 25.766973317237646 21.928984722973816 32 34.596447491430354 18.36501506180534 24.43751947647242 22.60101797029189 33 33.75777625200258 19.553502694380292 26.48398976343549 20.204731290181638 34 26.047642803691694 21.40392450320113 33.07350128876694 19.474931404340236 35 23.536012634553842 29.225717966834296 29.747308923153653 17.490960475458213 36 24.772188241168394 31.111388507468895 25.117546706611744 18.998876544750967 37 29.512905911740216 27.774771024146546 23.130724396336387 19.581598667776852 38 28.62045298558682 28.728603814396543 23.096441421767434 19.554501778249204 39 27.34039447896251 21.00829823610358 21.83603686251616 29.81527042241775 40 17.45570783887682 14.940247367541367 18.688367039946517 48.9156777536353 41 16.41947377241495 18.637925255572316 20.946455505279037 43.9961454667337 42 21.293867865617585 24.944839931726406 27.1200199825153 26.64127222014071 43 13.67104440275172 13.16447779862414 22.184698770064625 50.97977902855951 44 16.21920899233228 13.83323235066753 21.09353780582078 48.854020851179406 45 26.5430044917651 21.926468141740145 27.3041091332557 24.226418233239063 46 22.471676540900116 26.238436752936284 26.331982122440493 24.957904583723106 47 19.697032665613833 20.99160502036406 26.90965007065082 32.401712243371286 48 23.160519480519483 23.312207792207793 31.623896103896104 21.903376623376623 49 18.360539537598765 14.929013696916629 20.895615735818378 45.81483102966623 50 21.63585644100258 22.120909318011492 30.57914897160463 25.6640852693813 51 21.320939538567348 23.89279089639649 31.86469644234176 22.9215731226944 52 20.895615735818378 22.80943148414535 29.851773094002127 26.443179686034146 53 20.48950030177527 21.519698640970674 34.945576390767755 23.045224666486295 54 19.011462212144536 18.612053004930207 30.875163820182642 31.501320962742607 55 20.967876165113182 21.835469374167776 30.20972037283622 26.986934087882826 56 21.932946244615096 24.199288256227756 26.49060373353312 27.377161765624024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 0.5 13 9.0 14 17.0 15 29.5 16 42.0 17 53.0 18 64.0 19 91.5 20 119.0 21 123.0 22 148.0 23 169.0 24 198.5 25 228.0 26 240.5 27 253.0 28 334.0 29 450.5 30 486.0 31 578.0 32 670.0 33 736.5 34 803.0 35 799.5 36 796.0 37 817.0 38 845.0 39 852.0 40 817.5 41 783.0 42 806.5 43 830.0 44 852.0 45 874.0 46 905.5 47 1036.5 48 1136.0 49 1233.0 50 1330.0 51 1360.5 52 1391.0 53 1511.0 54 1774.0 55 1917.0 56 2157.5 57 2398.0 58 2749.5 59 3101.0 60 3417.5 61 3734.0 62 4106.5 63 4595.5 64 4712.0 65 4183.5 66 3655.0 67 3232.5 68 2810.0 69 2488.0 70 2166.0 71 1852.0 72 1306.5 73 1075.0 74 895.0 75 715.0 76 609.5 77 504.0 78 401.5 79 222.5 80 146.0 81 103.5 82 61.0 83 46.0 84 31.0 85 20.0 86 9.0 87 6.5 88 2.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.031153294980165735 10 0.0 11 0.0 12 0.0 13 0.0 14 0.02284574965212154 15 0.20145797420507175 16 0.04361461297223203 17 0.01869197698809944 18 0.008307545328044195 19 0.012461317992066293 20 0.03323018131217678 21 0.006230658996033147 22 0.0 23 0.0 24 0.0 25 0.0 26 0.17653533822093917 27 0.026999522316143637 28 0.18068911088496126 29 0.03738395397619888 30 0.03530706764418783 31 0.21391929219713807 32 0.029076408648154684 33 0.1786122245529502 34 0.08515233961245301 35 0.05607593096429832 36 0.17238156555691705 37 0.22638061018920436 38 0.14122827057675133 39 0.3883777440860661 40 0.591912604623149 41 0.8577540551205634 42 0.22222683752518227 43 0.3717626534299778 44 0.59398949095516 45 0.12669006625267398 46 0.09138299860848616 47 0.051922158300276224 48 0.04984527196826517 49 0.3779933124260109 50 0.23468815551724856 51 0.3489169037778562 52 0.3779933124260109 53 0.2076886332011049 54 0.16199713389686182 55 0.17653533822093917 56 0.20353486053708278 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 48149.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.26033770171757 #Duplication Level Percentage of deduplicated Percentage of total 1 93.01617460781448 79.30590458784191 2 4.460196823540874 7.605557747824461 3 1.120530059436812 2.8661031381752475 4 0.467699503069278 1.5950487029844855 5 0.23141381662281982 0.9865210077052483 6 0.18513105329825588 0.9470601673970384 7 0.13154048523823444 0.7850630335001765 8 0.060898372795478904 0.4153772664022098 9 0.05115463314820228 0.39253151675008824 >10 0.26064503556464974 3.8567779185445183 >50 0.009743739647276624 0.6106045816112484 >100 0.004871869823638312 0.6334503312633699 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 169 0.3509937901098673 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 136 0.28245654115350266 No Hit GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 86 0.1786122245529502 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC 76 0.15784336123283974 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 71 0.14745892957278448 No Hit GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 61 0.12669006625267398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002076886332011049 0.0 0.0 0.0 0.0 11 0.002076886332011049 0.0 0.0 0.0 0.0 12 0.004153772664022098 0.0 0.0 0.0 0.0 13 0.004153772664022098 0.0 0.0 0.0 0.0 14 0.004153772664022098 0.0 0.0 0.0 0.0 15 0.004153772664022098 0.0 0.0 0.0 0.0 16 0.0062306589960331475 0.0 0.0 0.0 0.0 17 0.008307545328044195 0.0 0.0 0.0 0.0 18 0.012461317992066295 0.0 0.0 0.0 0.0 19 0.01661509065608839 0.0 0.0 0.0 0.0 20 0.02284574965212154 0.0 0.0 0.0 0.0 21 0.02284574965212154 0.0 0.0 0.0 0.0 22 0.02284574965212154 0.0 0.0 0.0 0.0 23 0.02492263598413259 0.0 0.0 0.0 0.0 24 0.02492263598413259 0.0 0.0 0.0 0.0 25 0.026999522316143637 0.0 0.0 0.0 0.0 26 0.031153294980165735 0.0 0.0 0.0 0.0 27 0.031153294980165735 0.0 0.0 0.0 0.0 28 0.03530706764418783 0.0 0.0 0.0 0.0 29 0.03530706764418783 0.0 0.0 0.0 0.0 30 0.03530706764418783 0.0 0.0 0.0 0.0 31 0.03738395397619888 0.0 0.0 0.0 0.0 32 0.03738395397619888 0.0 0.0 0.0 0.0 33 0.03738395397619888 0.0 0.0 0.0 0.0 34 0.03738395397619888 0.0 0.0 0.0 0.0 35 0.03738395397619888 0.0 0.0 0.0 0.0 36 0.03738395397619888 0.0 0.0 0.0 0.0 37 0.039460840308209934 0.0 0.0 0.0 0.0 38 0.039460840308209934 0.0 0.0 0.0 0.0 39 0.039460840308209934 0.0 0.0 0.0 0.0 40 0.039460840308209934 0.0 0.0 0.0 0.0 41 0.04153772664022098 0.0 0.0 0.0 0.0 42 0.04153772664022098 0.0 0.0 0.0 0.0 43 0.04153772664022098 0.0 0.0 0.0 0.0 44 0.04153772664022098 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACTA 15 0.007902427 50.749733 39 AGCACCG 15 0.007902427 50.749733 39 GCACCGA 15 0.007902427 50.749733 40 CTGCACT 15 0.007902427 50.749733 38 GATACCC 15 0.008137226 50.37579 35 TAAGATC 20 3.9929216E-4 50.37579 35 ATACCCA 15 0.008137226 50.37579 36 TCGACCC 15 0.008205255 50.26996 43 ACCATGT 15 0.008205255 50.26996 42 TCGCCTA 15 0.008205255 50.26996 42 CCTGATT 15 0.008239427 50.21721 49 TCCGACG 15 0.008239427 50.21721 49 CTCAAAG 15 0.008239427 50.21721 44 CAATAGC 15 0.008273705 50.16457 45 TCAAAGA 15 0.008273705 50.16457 45 ATAGCAG 15 0.008273705 50.16457 47 AATAGCA 15 0.008273705 50.16457 46 ATGTTCC 15 0.008273705 50.16457 45 CGCGCCG 15 0.008273705 50.16457 47 CTACCAA 25 2.0176443E-5 50.164566 46 >>END_MODULE