FastQCFastQC Report
Fri 17 Jun 2016
SRR1526335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31338
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC860.2744272129682813No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC650.20741591677835217No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC600.19146084625694046No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC410.13083157827557598No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC360.11487650775416428No Hit
CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG330.10530346544131725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCAA150.00791552350.68617640
AACTAAA150.00791552350.68617638
TAAGCCC150.008121490550.3586436
GCGGCTC150.00817359950.27741635
ACCGACT204.0097415E-450.27741642
TTGCCTA204.0097415E-450.27741642
TGCCTAG204.0418567E-450.1964643
CCAAAAC650.050.19645743
CCGTGCG150.00827856350.11575347
GCAAGCA150.00827856350.11575347
ATGAAGG150.00827856350.11575348
TAGCAAG252.0128273E-550.11575347
ACCTGCG150.00827856350.11575334
CTGCGCT150.00827856350.11575349
CTAGCAA252.0128273E-550.11575346
CGTGCGC150.00827856350.11575348
CGACCGT150.00827856350.11575344
CCTATAT204.1067E-450.03531313
GCCTATA204.1067E-450.03531312
TGCCTAT204.1067E-450.03531311