##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526335_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31338 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.02236900887102 38.0 38.0 39.0 35.0 40.0 2 37.50197842874466 38.0 38.0 39.0 35.0 40.0 3 37.08791243857298 38.0 37.0 39.0 33.0 40.0 4 36.83125917416555 38.0 35.0 39.0 32.0 40.0 5 37.083253557980726 38.0 37.0 39.0 33.0 40.0 6 36.29934903312272 38.0 35.0 39.0 31.0 40.0 7 37.422330716701765 38.0 38.0 39.0 35.0 40.0 8 37.13239517518667 38.0 37.0 39.0 33.0 40.0 9 37.19213095921884 38.0 38.0 39.0 34.0 40.0 10 35.93806241623588 38.0 35.0 39.0 31.0 40.0 11 33.298423639032485 35.0 30.0 39.0 24.0 40.0 12 31.828131980343354 35.0 28.0 39.0 16.0 40.0 13 31.491799093751993 35.0 28.0 38.0 14.0 40.0 14 31.311347246154828 35.0 27.0 38.0 13.0 40.0 15 32.15214755249218 36.0 29.0 38.0 17.0 40.0 16 32.67202757036186 36.0 30.0 39.0 18.0 40.0 17 32.78932924883528 36.0 30.0 39.0 19.0 40.0 18 32.88391090688621 36.0 30.0 39.0 20.0 40.0 19 33.93123364605272 36.0 31.0 39.0 25.0 40.0 20 33.905322611525946 37.0 31.0 39.0 24.0 40.0 21 35.537558236007406 38.0 35.0 39.0 28.0 40.0 22 35.185270278894635 38.0 35.0 39.0 27.0 40.0 23 35.37373157189355 38.0 35.0 39.0 28.0 40.0 24 34.96824940966239 38.0 34.0 39.0 26.0 40.0 25 35.20479928521284 38.0 35.0 39.0 27.0 40.0 26 35.58022209458166 38.0 35.0 39.0 29.0 40.0 27 35.362148190695 38.0 35.0 39.0 28.0 40.0 28 35.44237028527666 38.0 35.0 39.0 29.0 40.0 29 35.22270087433787 38.0 35.0 39.0 28.0 40.0 30 35.045152849575594 38.0 35.0 39.0 27.0 40.0 31 34.49208628502138 38.0 34.0 39.0 26.0 40.0 32 33.38490012125854 38.0 33.0 39.0 18.0 40.0 33 34.625694045567684 38.0 34.0 39.0 26.0 40.0 34 34.351713574574 38.0 33.0 39.0 25.0 40.0 35 34.42714914799923 38.0 34.0 39.0 26.0 40.0 36 32.54649307549939 36.0 31.0 38.0 23.0 39.0 37 32.35184121513817 35.0 31.0 38.0 21.0 39.0 38 31.62805539600485 35.0 30.0 38.0 17.0 39.0 39 29.58985895717659 34.0 27.0 38.0 6.0 39.0 40 25.796413300146785 32.0 13.0 37.0 4.0 39.0 41 22.576265237092347 26.0 10.0 33.0 4.0 37.0 42 26.01209394345523 30.0 22.0 34.0 4.0 37.0 43 22.1078243665837 26.0 9.0 33.0 4.0 37.0 44 22.98714021315974 28.0 10.0 33.0 4.0 37.0 45 25.640627991575723 30.0 21.0 34.0 4.0 38.0 46 25.37689067585679 30.0 17.0 36.0 4.0 38.0 47 24.68590848171549 30.0 11.0 36.0 4.0 38.0 48 24.61506796860042 30.0 12.0 35.0 4.0 38.0 49 22.027474631437872 26.0 5.0 35.0 4.0 38.0 50 24.514423383751357 30.0 10.0 35.0 4.0 38.0 51 26.03752632586636 32.0 4.0 38.0 4.0 40.0 52 25.71529772161593 32.0 4.0 38.0 4.0 40.0 53 25.396387772033954 32.0 4.0 38.0 4.0 39.0 54 24.486119088646372 31.0 4.0 37.0 4.0 39.0 55 24.680898589571765 31.0 4.0 37.0 4.0 39.0 56 24.332216478396834 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.09091802500140744 1 2 0.39160899120921044 1 3 0.931722041626827 1 4 0.7497459628862018 1 5 0.9009587553756973 1 6 1.927549403461164 1 7 1.0612843981131235 1 8 1.3083422040446138 1 9 1.2570119066954177 1 10 1.4646161481734126 1 11 -3.900172093855822 1 12 -6.563527520429773 1 13 -21.478992650514343 1 14 -18.554204719725867 1 15 -12.0863123652763 1 16 -21.564372465958662 1 17 -23.303693374950115 1 18 -20.934346859120392 1 19 -5.613308975988961 1 20 -11.512254355262915 1 21 -6.784038493047959 1 22 -2.9068712533559378 1 23 -2.839674934630157 1 24 -2.17740568813473 1 25 -3.2356937780033093 1 26 2.8259242141268928 1 27 2.970731581842628 1 28 2.922512111479513 1 29 2.188148742504673 1 30 2.8328164162491305 1 31 1.645123802128758 1 32 -11.92054375385084 1 33 2.1311995871964413 1 34 1.6280098064242594 1 35 2.409272139009019 1 36 4.678758289161774 1 37 4.3657678116709135 1 38 5.556105442958533 1 39 6.135578254411087 1 40 7.571460662653401 1 41 -1.0199812502714423 1 42 -8.16162398502383 1 43 -14.66797912691383 1 44 -16.28232135949702 1 45 -15.15968689008243 1 46 -18.22451793156545 1 47 -18.452259935196032 1 48 -15.919650992381037 1 49 -13.493005482663273 1 50 -16.137038685231232 1 51 -17.221611424961164 1 52 -16.968607967359258 1 53 -17.214516016354693 1 54 -18.481144207052317 1 55 -17.50624200619489 1 56 -16.77565319058728 2 1 -0.5266323107156907 2 2 0.21303756263777984 2 3 0.653150613055395 2 4 -0.2038254656852274 2 5 0.12238732680426523 2 6 0.8096922606040238 2 7 0.6005701123988345 2 8 0.047627918330327645 2 9 0.3712976209811316 2 10 0.9753304338876987 2 11 -6.560886379570107 2 12 -14.792098949001202 2 13 -15.218278364800058 2 14 -17.53634757686872 2 15 -9.475598079562015 2 16 -3.5286581802443777 2 17 -2.8394076606644028 2 18 -11.220061144834677 2 19 -2.4097375474175315 2 20 -4.508682926691485 2 21 -0.10903849304796154 2 22 1.0002716037869206 2 23 0.7174679225126965 2 24 0.49045145472241103 2 25 1.0035919362824046 2 26 0.9759242141268984 2 27 0.8421601532712018 2 28 0.5510835400509393 2 29 1.131005885361816 2 30 1.1471021305348472 2 31 -2.1013047692998157 2 32 -13.266972325279411 2 33 -2.7545146985178484 2 34 -2.046990193575745 2 35 -1.8692992895624094 2 36 2.178758289161774 2 37 3.0764820973852025 2 38 3.359676871529963 2 39 5.735578254411088 2 40 8.217889234081973 2 41 6.9728758925857015 2 42 1.0276617292618866 2 43 -0.06440769834240001 2 44 -10.571607073782733 2 45 0.21888453848899658 2 46 -12.338803645851165 2 47 -13.723688506624603 2 48 -9.698222420952467 2 49 -12.682291196948988 2 50 -9.379895828088372 2 51 -8.421611424961164 2 52 -8.890036538787829 2 53 -8.639516016354696 2 54 -14.659715635623744 2 55 -8.231242006194886 2 56 -7.647081762015853 3 1 0.5033676892843033 3 2 0.983037562637783 3 3 1.5431506130553956 3 4 0.8411745343147743 3 5 1.4723873268042666 3 6 1.9246922606040258 3 7 1.7755701123988317 3 8 1.6226279183303234 3 9 1.731297620981131 3 10 1.9903304338876993 3 11 0.8941136204298914 3 12 1.3879010509987992 3 13 -3.6732783648000584 3 14 -4.651347576868723 3 15 -2.460598079562015 3 16 3.1213418197556244 3 17 2.5305923393355947 3 18 3.1199388551653193 3 19 1.0552624525824683 3 20 1.5763170733085161 3 21 2.5359615069520416 3 22 3.5102716037869186 3 23 3.757467922512703 3 24 3.345451454722415 3 25 3.1585919362824058 3 26 2.7859242141268936 3 27 3.182160153271198 3 28 2.8710835400509396 3 29 2.691005885361818 3 30 3.087102130534845 3 31 3.453695230700184 3 32 1.468027674720588 3 33 3.3654853014821526 3 34 3.508009806424255 3 35 3.140700710437592 3 36 4.938758289161772 3 37 5.7114820973852005 3 38 6.224676871529965 3 39 8.055578254411088 3 40 10.482889234081973 3 41 8.502875892585703 3 42 7.332661729261886 3 43 3.3605923016576007 3 44 4.523392926217269 3 45 6.788884538488997 3 46 0.3211963541488352 3 47 -4.683688506624602 3 48 0.17177757904753221 3 49 -9.607291196948987 3 50 -0.7398958280883718 3 51 -1.4216114249611636 3 52 -2.0000365387878283 3 53 -1.8545160163546939 3 54 -6.8097156356237445 3 55 -1.4662420061948858 3 56 0.13291823798414626 4 1 0.7819883789394808 4 2 1.2009685971205428 4 3 1.4134954406416043 4 4 1.3211745343147712 4 5 1.3654907750801328 4 6 2.4088301916385078 4 7 1.8548804572264217 4 8 2.153662401088944 4 9 2.223021758912161 4 10 3.015847675267011 4 11 5.1244584480160995 4 12 5.504452775136727 4 13 6.099825083475807 4 14 4.357617940372659 4 15 6.147677782506953 4 16 6.78341078527286 4 17 5.979557856576978 4 18 5.593731958613599 4 19 4.4980210732721275 4 20 4.0473515560671345 4 21 3.6987201276416997 4 22 3.9468233279248466 4 23 3.4519506811333898 4 24 3.9654514547224124 4 25 3.375143660420335 4 26 3.1293724899889668 4 27 2.7111256705125797 4 28 3.2200490572923215 4 29 2.486178299154922 4 30 3.5126193719141554 4 31 3.7047297134588035 4 32 4.059751812651619 4 33 3.45927840493043 4 34 3.841802909872534 4 35 2.025528296644488 4 36 5.020137599506601 4 37 4.572171752557615 4 38 5.281228595667891 4 39 7.925233426824882 4 40 8.787716820288871 4 41 5.171151754654666 4 42 6.887144487882576 4 43 7.605419887864496 4 44 9.775806719320716 4 45 7.672332814351066 4 46 8.806023940355733 4 47 9.00389770027195 4 48 7.624191372150982 4 49 4.682363975464806 4 50 8.578724861566798 4 51 7.571492023314697 4 52 6.99099794397079 4 53 6.868242604334959 4 54 2.8247671229969455 4 55 7.317895924839597 4 56 7.711538927639317 5 1 0.4914527956672856 5 2 0.4992077754037396 5 3 1.0180442300766757 5 4 1.0658553853785975 5 5 1.029834135314907 5 6 1.1634156648593432 5 7 1.613867984739258 5 8 1.7970960034367067 5 9 1.4266167699173025 5 10 1.4013942636749377 5 11 5.133262556600108 5 12 7.18236913610518 5 13 6.700338656476539 5 14 6.34184391249298 5 15 6.135572133203944 5 16 6.240490755925837 5 17 5.772294466995174 5 18 5.009726089207877 5 19 4.611007133433532 5 20 4.073763881819154 5 21 2.7265998048243816 5 22 2.6992077739996816 5 23 3.0770423905978035 5 24 2.4335365611053916 5 25 2.4998685320270866 5 26 2.9408178311481734 5 27 2.4400324936967337 5 28 2.087253752816899 5 29 2.5650484385533048 5 30 2.674761705002929 5 31 2.2524186349555038 5 32 3.805900015146122 5 33 3.0880384929715134 5 34 3.7258821468497914 5 35 2.0743177317141885 5 36 3.6617370125660322 5 37 3.4089289058958414 5 38 5.022974743870385 5 39 6.31004633951747 5 40 5.5811871064224015 5 41 4.254790786202722 5 42 8.735640452666143 5 43 12.441443365487384 5 44 11.518286543238546 5 45 9.919948368276234 5 46 10.780345290319051 5 47 10.920992344439224 5 48 11.664543536494339 5 49 12.694836462625478 5 50 11.42648715063503 5 51 10.267750277166495 5 52 11.464218780361108 5 53 11.555696749602749 5 54 11.311560960120936 5 55 11.540566504443412 5 56 9.963556535856487 6 1 0.610324211023439 6 2 0.5630375626377813 6 3 0.9074984391423513 6 4 1.147261490836513 6 5 1.0506481963694867 6 6 1.7064313910388051 6 7 1.4755701123988345 6 8 1.4617583531129341 6 9 1.6060802296767775 6 10 2.2242434773659596 6 11 5.596722316082065 6 12 7.238335833607493 6 13 7.02411293954777 6 14 7.766913292696497 6 15 6.960271485655376 6 16 6.486559211059973 6 17 6.149722774118207 6 18 5.332112768208798 6 19 4.77613201779986 6 20 5.159795334178078 6 21 2.8463962895607366 6 22 3.3763585603086597 6 23 2.8087722703387854 6 24 3.095886237331108 6 25 2.7829397623693595 6 26 3.0694024749964584 6 27 1.4899862402277222 6 28 2.1510835400509407 6 29 1.5266580592748653 6 30 2.6992760435783225 6 31 1.708477839395833 6 32 3.268897239937978 6 33 2.9367896493082384 6 34 3.2106185020764286 6 35 1.832874623481068 6 36 3.6265843761182985 6 37 2.4080038365156398 6 38 4.4281551323995245 6 39 6.01818695006326 6 40 4.660280538429799 6 41 3.8098324143248306 6 42 8.135270424914058 6 43 13.543200997309771 6 44 12.125132056652049 6 45 9.481928016749865 6 46 11.482935484583617 6 47 11.828485406418874 6 48 10.60395149209101 6 49 13.470969672616231 6 50 10.820104171911627 6 51 10.017519009821445 6 52 10.581702591646952 6 53 10.798527461906172 6 54 11.703327842637123 6 55 10.528540602500762 6 56 8.865961716245017 7 1 1.2678121337287536 7 2 1.6963708959711141 7 3 1.7031506130553993 7 4 2.1878412009814383 7 5 2.072387326804268 7 6 2.9624700383818023 7 7 1.8644590012877202 7 8 2.222627918330325 7 9 2.39351984320335 7 10 3.0614415449988144 7 11 5.7252247315410045 7 12 7.341234384332132 7 13 7.340054968533273 7 14 7.38865242313128 7 15 7.354957475993544 7 16 7.121341819755624 7 17 6.4550367837800415 7 18 6.468827744054213 7 19 4.881929119249136 7 20 5.5385392955307395 7 21 3.793739284729817 7 22 3.394716048231359 7 23 3.750801255846035 7 24 4.298784788055748 7 25 3.7452586029490718 7 26 3.314813103015787 7 27 3.4310490421600903 7 28 3.1510835400509407 7 29 3.3532281075840444 7 30 3.1915465749792915 7 31 4.118139675144626 7 32 5.508027674720587 7 33 4.112151968148822 7 34 3.7391209175353666 7 35 3.6029229326598156 7 36 3.034313844717328 7 37 3.2737043196074254 7 38 3.8957879826410746 7 39 6.491133809966641 7 40 -1.7237774325846935 7 41 -9.766012996303187 7 42 4.70821728481744 7 43 10.5605923016576 7 44 8.536726259550601 7 45 6.557773427377885 7 46 9.038974131926611 7 47 9.220755937819842 7 48 9.985110912380865 7 49 11.037153247495457 7 50 9.431215283022741 7 51 11.533944130594389 7 52 11.259963461212173 7 53 11.318817316978635 7 54 12.64583991993181 7 55 11.404869104916223 7 56 9.608473793539705 8 1 0.5733676892843036 8 2 0.7630375626377841 8 3 1.4281506130554007 8 4 1.146174534314774 8 5 1.322387326804268 8 6 1.7096922606040224 8 7 1.6505701123988317 8 8 1.6226279183303234 8 9 1.6962976209811274 8 10 1.7253304338876987 8 11 4.5391136204298945 8 12 7.632901050998797 8 13 7.956721635199941 8 14 8.238652423131281 8 15 7.774401920437988 8 16 7.096341819755619 8 17 5.660592339335597 8 18 6.254938855165324 8 19 5.415262452582468 8 20 5.366317073308515 8 21 3.5159615069520385 8 22 4.200271603786916 8 23 2.767467922512701 8 24 4.690451454722414 8 25 3.428591936282409 8 26 2.375924214126897 8 27 2.442160153271196 8 28 2.4010835400509407 8 29 2.7810058853618216 8 30 3.347102130534843 8 31 3.1486952307001843 8 32 4.508027674720587 8 33 3.695485301482151 8 34 4.028009806424258 8 35 2.9807007104375884 8 36 0.6537582891617753 8 37 0.4014820973852018 8 38 0.8346768715299611 8 39 3.4605782544110895 8 40 -1.657110765918027 8 41 -11.227124107414298 8 42 3.552661729261885 8 43 8.7355923016576 8 44 7.753392926217266 8 45 3.4938845384889987 8 46 6.786196354148835 8 47 7.976311493375398 8 48 8.176777579047535 8 49 10.692708803051012 8 50 7.570104171911627 8 51 8.853388575038839 8 52 9.05996346121217 8 53 10.035483983645303 8 54 9.465284364376252 8 55 9.218757993805113 8 56 7.477918237984145 9 1 -0.08532796288960753 9 2 -0.06739721997091408 9 3 0.4074984391423513 9 4 0.49508757779302925 9 5 0.26803950071731464 9 6 0.7064313910388051 9 7 0.6494831558770926 9 8 0.8748018313738015 9 9 0.73651501228548 9 10 1.4416347817137876 9 11 4.96628753347337 9 12 6.064422790129235 9 13 6.415417287373856 9 14 6.658217640522583 9 15 6.242880181307548 9 16 6.551776602364317 9 17 5.345374948031246 9 18 5.636460594295759 9 19 4.232653756930297 9 20 3.9206648993954687 9 21 2.0203093330389947 9 22 2.463315082047785 9 23 1.8305114007735668 9 24 2.508929715591975 9 25 1.5003310667171874 9 26 1.4172285619529816 9 27 1.7073775445755501 9 28 2.6945618009205035 9 29 1.961440667970514 9 30 1.6340586522739784 9 31 2.4041300133088797 9 32 3.5732450660249384 9 33 2.3715722580038943 9 34 2.7975750238155612 9 35 2.0285267973941146 9 36 0.9091930717704706 9 37 2.1688734017330304 9 38 1.7759812193560478 9 39 2.822534776150217 9 40 -0.03537163548324429 9 41 -5.211906716109951 9 42 3.9830965118705812 9 43 9.738853171222818 9 44 7.168610317521615 9 45 5.894971495010736 9 46 8.591631136757531 9 47 8.11109410207105 9 48 9.169168883395358 9 49 11.731839237833622 9 50 9.01575634582467 9 51 9.126214661995359 9 52 9.886050417733912 9 53 9.385483983645305 9 54 8.703327842637126 9 55 8.485062341631199 9 56 7.0616138901580605 10 1 0.9983676892843079 10 2 1.3380375626377798 10 3 1.8781506130553964 10 4 2.021174534314774 10 5 1.6223873268042652 10 6 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2.9490011372038154 114 27 3.219083230194279 114 28 2.728006616974021 114 29 3.2540828084387385 114 30 3.3547944382271524 114 31 2.542925999930951 114 32 -1.6842800175871062 114 33 3.276254532251386 114 34 2.9280098064242566 114 35 2.8730084027452847 114 36 4.971065981469469 114 37 4.759174405077509 114 38 5.692369179222268 114 39 7.812501331334168 114 40 11.250581541774281 114 41 10.492106661816468 114 42 8.08343096003112 114 43 6.414438455503753 114 44 3.1803160031403443 114 45 5.761192230796691 114 46 -3.0772651843127043 114 47 -4.708303891239986 114 48 -2.74053011326016 114 49 -13.007291196948987 114 50 -3.0645112127037564 114 51 -0.6831498864996242 114 52 0.3291942304429405 114 53 -0.46066986250854214 114 54 0.44413051822240845 114 55 0.593757993805113 114 56 4.4529182379841465 115 1 -0.053555387638773766 115 2 0.17842217802239446 115 3 0.9108429207477045 115 4 0.43655914969938436 115 5 0.6877719421888813 115 6 1.338538414450177 115 7 -0.02442988760116549 115 8 0.4918586875610913 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31 -0.7263047692998157 118 32 -8.752841890496804 118 33 -0.9545146985178476 118 34 -0.658946715314876 118 35 -2.2975601591276273 118 36 2.2787582891617753 118 37 1.2340907930373746 118 38 3.55858991500822 118 39 5.56166521093283 118 40 5.116802277560236 118 41 4.157658501281354 118 42 1.3091834683923196 118 43 2.4997227364402086 118 44 2.125132056652049 118 45 0.2862758428368224 118 46 -4.8866297328076875 118 47 -6.301949376189821 118 48 -9.33083111660464 118 49 -7.050769457818552 118 50 -6.549461045479678 118 51 -7.221611424961164 118 52 -7.461775669222611 118 53 -7.136255146789477 118 54 -9.101019983449833 118 55 -7.732328962716625 118 56 -3.416646979407158 119 1 -0.6129959470793267 119 2 -0.07332607372585187 119 3 0.012241522146304362 119 4 0.18481089795113803 119 5 0.34511459953154144 119 6 0.6846922606040238 119 7 0.021024657853381257 119 8 -0.2773720816696752 119 9 -0.055975106291597854 119 10 -1.176942293385025 119 11 -8.95861365229738 119 12 -8.573917130819385 119 13 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119 51 -4.085247788597528 119 52 -3.3491274478787396 119 53 -6.750879652718332 119 54 -9.482442908351018 119 55 -6.724423824376704 119 56 -4.5925363074704 120 1 -0.7039050379884202 120 2 -1.391507891907672 120 3 -0.7150312051264223 120 4 -0.9060981929579555 120 5 -0.745794491377552 120 6 -0.4971259212141561 120 7 -1.3426117057829856 120 8 -1.459190263487855 120 9 -1.3287023790188712 120 10 -2.222396838839572 120 11 -10.45861365229738 120 12 -4.301189858092112 120 13 -9.284187455709148 120 14 -5.347711213232358 120 15 -9.209688988652925 120 16 -9.533203634789832 120 17 -9.771225842482586 120 18 -5.910970235743768 120 19 -4.575646638326621 120 20 -5.610955653964211 120 21 -1.738583947593412 120 22 -1.5860920325767154 120 23 -4.218895713850937 120 24 -6.352730363459404 120 25 -2.1123171546266875 120 26 -0.6104394222367375 120 27 -0.8123853012742543 120 28 -0.2580073690399658 120 29 0.24009679445272525 120 30 -1.9074433240106075 120 31 -3.8626684056634524 120 32 -11.81015414346123 120 33 -2.6817874257905743 120 34 -14.253808375393927 120 35 -3.4102083804714987 120 36 -2.3121508017473147 120 37 -2.5030633571602507 120 38 -3.206232219379128 120 39 -0.3553308364980019 120 40 -2.5571107659180257 120 41 0.6319668016766116 120 42 -3.738247361647204 120 43 -2.3303167892514907 120 44 -1.4875161646918222 120 45 -7.476570006965549 120 46 -12.675167282214801 120 47 -12.73277941571551 120 48 -12.91185878458883 120 49 -14.689109378767169 120 50 -11.4071685553611 120 51 -12.085247788597528 120 52 -11.758218356969648 120 53 -12.432697834536514 120 54 -11.936988362896471 120 55 -12.542605642558524 120 56 -9.77435448928858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 69.0 5 11.0 6 58.0 7 42.0 8 90.0 9 112.0 10 63.0 11 49.0 12 63.0 13 65.0 14 94.0 15 101.0 16 156.0 17 207.0 18 252.0 19 331.0 20 419.0 21 516.0 22 599.0 23 717.0 24 822.0 25 949.0 26 959.0 27 1031.0 28 1111.0 29 1138.0 30 1418.0 31 1612.0 32 1854.0 33 2222.0 34 2497.0 35 2898.0 36 3252.0 37 3068.0 38 1878.0 39 615.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.4766736868977 0.2488991001340226 0.22337098729976385 0.05105622566851745 8 99.14799923415661 0.2840002552811284 0.4946071861637628 0.07339332439849384 9 97.60278345250255 0.8203524004085802 1.2353166496424923 0.34154749744637386 10 64.83502457080861 21.56806433084434 6.713893675410046 6.8830174229370105 11 37.64758440232306 26.833237602910202 18.929095666602848 16.59008232816389 12 42.118195162422616 11.379156295870828 22.139255855510882 24.363392686195674 13 28.789329248835283 11.525942944667815 26.944923096560085 32.73980470993682 14 27.163331098981775 13.913626352580676 26.496217562003256 32.42682498643429 15 24.248042192744126 14.821799584465397 27.19833786159501 33.73182036119546 16 29.705309536732543 19.93869927524664 26.94677692283133 23.40921426518949 17 36.24553115423902 17.709397344228805 21.973953013278855 24.071118488253322 18 30.032550421240746 20.155731427112585 27.56573908603523 22.245979065611436 19 34.16432584269663 20.44496935648621 20.65564351378958 24.73506128702758 20 28.079918294395505 22.066896463679306 23.018000765990042 26.835184475935147 21 27.99744694431147 20.012765278442636 29.67608105951811 22.31370671772778 22 29.35094773118897 19.46837705022656 25.41961835471313 25.76105686387134 23 29.816835790414192 19.966175250494608 31.12196055906567 19.09502840002553 24 35.88295360265492 18.281319803433533 23.2018635522369 22.633863041674644 25 31.536792392622377 19.761950347820537 25.671708468951433 23.029548790605656 26 33.63392171910975 20.133026349449988 22.78715784088002 23.445894090560245 27 27.120375395026652 16.458645896510994 28.47384045711367 27.947138251348676 28 32.26126154928227 19.815211483743088 23.114549697880367 24.80897726909428 29 32.27773343974461 22.266560255387073 21.873902633679172 23.581803671189146 30 31.737215092894083 21.91151120475005 24.85156100363915 21.49971269871672 31 30.115471963663115 23.14877011163356 25.06477305440937 21.670984870293957 32 32.11105792245093 21.113770544119994 23.976384234881124 22.79878729854795 33 31.672848190305665 22.947307839877222 25.738585496866605 19.641258472950504 34 27.96780684104628 23.630672926447573 30.149148861422503 18.252371371083644 35 24.944121591417076 28.839644932626605 28.363880196691998 17.85235327926432 36 24.156674660271783 29.90247801758593 27.114308553157475 18.82653876898481 37 24.257647510559323 30.929220529886088 24.884807372328172 19.928324587226417 38 27.208819300207697 29.96325291580125 24.975235660648664 17.852692123342386 39 24.51112393408989 23.805860101301533 22.690260947618132 28.992755016990447 40 16.87110654421681 17.808747029734764 19.089974953439086 46.230171472609335 41 18.645487643780005 19.518639043722008 20.997519090118246 40.83835422237974 42 20.915283507627365 27.89024273241869 28.728133294956663 22.46634046499728 43 14.175472302273457 14.642971501761126 23.326929234710214 47.85462696125521 44 15.653178448801464 16.038250489362387 21.069858486025094 47.23871257581106 45 24.24290825453616 25.686813186813183 28.02517250191669 22.045106056733964 46 22.64367816091954 27.755427841634738 27.29565772669221 22.30523627075351 47 21.39436889484773 22.84364425716657 26.568984230351784 29.193002617633912 48 22.531209092940838 27.160690910251905 29.16892819514064 21.139171801666613 49 17.723139966705084 16.97400435395057 19.826482264054295 45.47637341529005 50 22.15576523268831 25.523748600671674 30.7852230929154 21.53526307372461 51 22.316167050986422 27.581347681270817 28.676658980271586 21.425826287471175 52 19.596218554718796 28.78384874218875 28.899214869411953 22.7207178336805 53 21.116403977109243 27.44333258735893 31.343713034304166 20.09655040122766 54 18.719447905683886 23.17326432154382 28.738937346241094 29.368350426531197 55 20.440965010385046 26.863716248602014 30.330723757788785 22.364594983224155 56 20.888221000127892 26.947179946284688 28.02788080317176 24.136718250415655 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 4.0 13 12.5 14 17.0 15 31.0 16 45.0 17 55.0 18 65.0 19 78.5 20 92.0 21 93.5 22 115.5 23 136.0 24 151.5 25 167.0 26 238.0 27 309.0 28 360.5 29 485.5 30 559.0 31 626.5 32 694.0 33 704.5 34 715.0 35 691.0 36 667.0 37 663.5 38 658.0 39 656.0 40 642.0 41 628.0 42 644.5 43 661.0 44 688.0 45 715.0 46 736.5 47 803.5 48 849.0 49 878.5 50 908.0 51 979.0 52 1050.0 53 1132.5 54 1364.5 55 1514.0 56 1595.0 57 1676.0 58 1835.0 59 1994.0 60 2336.0 61 2678.0 62 2740.0 63 2616.0 64 2430.0 65 2170.5 66 1911.0 67 1664.0 68 1417.0 69 1192.0 70 967.0 71 838.0 72 587.0 73 465.0 74 369.5 75 274.0 76 246.5 77 219.0 78 164.5 79 79.5 80 49.0 81 37.0 82 25.0 83 18.5 84 12.0 85 7.5 86 3.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.031910141042823414 10 0.0 11 0.0 12 0.0 13 0.0 14 0.02871912693854107 15 0.16912374752696407 16 0.0542472397727998 17 0.031910141042823414 18 0.006382028208564682 19 0.031910141042823414 20 0.019146084625694046 21 0.009573042312847023 22 0.0 23 0.0 24 0.0 25 0.0 26 0.2106069308826345 27 0.035101155147105746 28 0.1882698321526581 29 0.04148318335567044 30 0.03829216925138809 31 0.23932605782117558 32 0.009573042312847023 33 0.19784287446550516 34 0.08615738081562321 35 0.06382028208564683 36 0.2010338885697875 37 0.2744272129682813 38 0.13721360648414066 39 0.4595060310166571 40 0.6254387644393389 41 0.9604952453889846 42 0.22017997319548152 43 0.34462952326249285 44 0.5584274682494097 45 0.1084944795455996 46 0.05743825387708214 47 0.03829216925138809 48 0.0542472397727998 49 0.3254834386367988 50 0.23294402961261088 51 0.36377560788818686 52 0.4244048758695514 53 0.1882698321526581 54 0.1244495500670113 55 0.13721360648414066 56 0.19784287446550516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 31338.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.78798902291149 #Duplication Level Percentage of deduplicated Percentage of total 1 93.46806731852713 82.05373667751611 2 4.300098142561158 7.549939370732019 3 1.1340918178183272 2.9867892016082713 4 0.43618916069935665 1.5316867700555237 5 0.1890153029697212 0.8296636671134087 6 0.1235869288648177 0.6509668772735976 7 0.06906328377739814 0.4244048758695514 8 0.06542837410490349 0.4595060310166571 9 0.036349096724946385 0.2871912693854107 >10 0.16720584493475338 2.5528112834258727 >50 0.010904729017483916 0.673303976003574 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 86 0.2744272129682813 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 65 0.20741591677835217 No Hit CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 60 0.19146084625694046 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 41 0.13083157827557598 No Hit CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 36 0.11487650775416428 No Hit CTGTGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 33 0.10530346544131725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.003191014104282341 0.0 0.0 0.0 0.0 10 0.003191014104282341 0.0 0.0 0.0 0.0 11 0.009573042312847023 0.0 0.0 0.0 0.0 12 0.009573042312847023 0.0 0.0 0.0 0.0 13 0.015955070521411704 0.0 0.0 0.0 0.0 14 0.019146084625694046 0.0 0.0 0.0 0.0 15 0.019146084625694046 0.0 0.0 0.0 0.0 16 0.022337098729976388 0.0 0.0 0.0 0.0 17 0.025528112834258727 0.0 0.0 0.0 0.0 18 0.025528112834258727 0.0 0.0 0.0 0.0 19 0.025528112834258727 0.0 0.0 0.0 0.0 20 0.025528112834258727 0.0 0.0 0.0 0.0 21 0.03510115514710575 0.0 0.0 0.0 0.0 22 0.04148318335567043 0.0 0.0 0.0 0.0 23 0.04148318335567043 0.0 0.0 0.0 0.0 24 0.047865211564235115 0.0 0.0 0.0 0.0 25 0.0542472397727998 0.0 0.0 0.0 0.0 26 0.05743825387708214 0.0 0.0 0.0 0.0 27 0.06382028208564682 0.0 0.0 0.0 0.0 28 0.0702023102942115 0.0 0.0 0.0 0.0 29 0.0702023102942115 0.0 0.0 0.0 0.0 30 0.0702023102942115 0.0 0.0 0.0 0.0 31 0.07339332439849384 0.0 0.0 0.0 0.0 32 0.07339332439849384 0.0 0.0 0.0 0.0 33 0.07339332439849384 0.0 0.0 0.0 0.0 34 0.07339332439849384 0.0 0.0 0.0 0.0 35 0.07339332439849384 0.0 0.0 0.0 0.0 36 0.07339332439849384 0.0 0.0 0.0 0.0 37 0.07339332439849384 0.0 0.0 0.0 0.0 38 0.07339332439849384 0.0 0.0 0.0 0.0 39 0.07658433850277618 0.0 0.0 0.0 0.0 40 0.07658433850277618 0.0 0.0 0.0 0.0 41 0.0861573808156232 0.0 0.0 0.0 0.0 42 0.09253940902418789 0.0 0.0 0.0 0.0 43 0.09573042312847023 0.0 0.0 0.0 0.0 44 0.1021124513370349 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACCAA 15 0.007915523 50.686176 40 AACTAAA 15 0.007915523 50.686176 38 TAAGCCC 15 0.0081214905 50.35864 36 GCGGCTC 15 0.008173599 50.277416 35 ACCGACT 20 4.0097415E-4 50.277416 42 TTGCCTA 20 4.0097415E-4 50.277416 42 TGCCTAG 20 4.0418567E-4 50.19646 43 CCAAAAC 65 0.0 50.196457 43 CCGTGCG 15 0.008278563 50.115753 47 GCAAGCA 15 0.008278563 50.115753 47 ATGAAGG 15 0.008278563 50.115753 48 TAGCAAG 25 2.0128273E-5 50.115753 47 ACCTGCG 15 0.008278563 50.115753 34 CTGCGCT 15 0.008278563 50.115753 49 CTAGCAA 25 2.0128273E-5 50.115753 46 CGTGCGC 15 0.008278563 50.115753 48 CGACCGT 15 0.008278563 50.115753 44 CCTATAT 20 4.1067E-4 50.035313 13 GCCTATA 20 4.1067E-4 50.035313 12 TGCCTAT 20 4.1067E-4 50.035313 11 >>END_MODULE