FastQCFastQC Report
Fri 17 Jun 2016
SRR1526334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59308
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1190.20064746745801576No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1050.1770418830511904No Hit
GGGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA750.12645848789370742No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC650.10959735617454643No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC640.10791124300263033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCA451.1641532E-1050.65523539
CACACAC150.00797027650.6552340
TTATTCT150.00797027650.6552338
TAGTTTT150.00797027650.6552339
CCTCATG150.00797027650.6552340
TATTTAT150.00797027650.6552338
CCTGTGC203.9092917E-450.6118241
TTATTTA251.9690482E-550.39590537
TTTATTC203.9927804E-450.395937
CACACTG150.00818890750.3100542
CCAACAC900.050.3100543
GCCTTGA150.00818890750.3100542
GCCTAGC451.2369128E-1050.3100544
TGCCTAG451.2369128E-1050.3100543
TATTTTC251.9890505E-550.3100543
GTGCGTA150.00818890750.3100544
CATACCT150.00818890750.3100544
TGTGCGT150.00818890750.3100543
CGACTCC150.00818890750.3100544
CTTCTCT550.050.3100544