FastQCFastQC Report
Fri 17 Jun 2016
SRR1526333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12832
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC160.12468827930174563No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC160.12468827930174563No Hit
ACACCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA150.11689526184538654No Hit
ACACCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCCCGCAGCATTTGATATG140.10910224438902742No Hit
ACACCCGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA130.10130922693266833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACAAA203.6427594E-451.02016440
ACAAACC150.00760478951.0201638
CAAACCA150.00760478951.0201639
TTGATAC203.9445664E-450.2103233
CAGCAAC204.023036E-450.0118621
CAACATC204.023036E-450.0118624
GCAACAT204.023036E-450.0118623
TCAGCAA204.023036E-450.0118620
AGCAACA204.023036E-450.0118622
CTTTTGA150.00823305550.01185630
ATCCTGC150.00823305550.01185620
TTTTGAT150.00823305550.01185631
ATCTTTT150.00823305550.01185628
CCTGCCA150.00823305550.01185622
GCGGTTA150.00823305550.01185630
ACATCTT150.00823305550.01185626
AGCGGTT150.00823305550.01185629
TCTTTTG150.00823305550.01185629
CGGTTAA150.00823305550.01185631
TCCTGCC150.00823305550.01185621
Produced by FastQC (version 0.11.5)