##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526333_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12832 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.43259039900249 39.0 38.0 39.0 35.0 40.0 2 36.97428304239401 39.0 38.0 39.0 33.0 40.0 3 36.894404613466335 39.0 38.0 39.0 33.0 40.0 4 36.55049875311721 39.0 38.0 39.0 32.0 40.0 5 36.960645261845386 39.0 38.0 39.0 35.0 40.0 6 36.77454800498753 39.0 38.0 39.0 33.0 40.0 7 36.1849283042394 38.0 36.0 39.0 32.0 40.0 8 36.37913029925187 39.0 36.0 39.0 32.0 40.0 9 36.57153990024938 39.0 38.0 39.0 33.0 40.0 10 36.00202618453865 39.0 36.0 39.0 30.0 40.0 11 34.78000311720698 38.0 34.0 39.0 27.0 40.0 12 32.442721321695764 36.0 30.0 39.0 17.0 40.0 13 31.913887157107233 36.0 29.0 39.0 13.0 40.0 14 31.499064837905237 36.0 28.0 39.0 12.0 40.0 15 32.19381234413965 36.0 30.0 39.0 16.0 40.0 16 32.02758728179551 36.0 29.0 39.0 15.0 40.0 17 32.13778054862843 36.0 30.0 38.0 17.0 40.0 18 32.3033042394015 36.0 30.0 38.0 19.0 40.0 19 33.06756546134663 36.0 31.0 38.0 23.0 40.0 20 33.0413029925187 36.0 30.0 38.0 23.0 40.0 21 34.573955735660846 38.0 34.0 39.0 26.0 40.0 22 34.28732855361596 38.0 33.0 39.0 25.0 40.0 23 34.377259975062344 38.0 33.0 39.0 25.0 40.0 24 34.028990024937656 38.0 33.0 39.0 23.0 40.0 25 34.225140274314214 38.0 33.0 39.0 25.0 40.0 26 34.463606608478806 38.0 35.0 39.0 26.0 40.0 27 34.39370324189526 38.0 35.0 39.0 25.0 40.0 28 34.22210099750623 38.0 34.0 39.0 25.0 40.0 29 34.035614089775564 38.0 33.0 39.0 24.0 40.0 30 33.97451683291771 38.0 33.0 39.0 23.0 40.0 31 33.44131857855361 38.0 33.0 39.0 22.0 40.0 32 32.32831982543641 37.0 31.0 39.0 12.0 40.0 33 33.495713840399006 38.0 33.0 39.0 23.0 40.0 34 33.138092269326684 37.0 33.0 39.0 19.0 40.0 35 33.14222256857855 37.0 33.0 39.0 18.0 40.0 36 31.10497194513716 35.0 30.0 38.0 4.0 39.0 37 30.823644014962593 35.0 30.0 38.0 4.0 39.0 38 30.237764962593516 34.0 29.0 38.0 4.0 39.0 39 28.37453241895262 33.0 24.0 37.0 4.0 39.0 40 24.645339775561098 30.0 10.0 36.0 4.0 39.0 41 21.316318578553616 24.0 8.0 33.0 4.0 36.0 42 24.67690149625935 30.0 17.0 33.0 4.0 37.0 43 21.059928304239403 25.0 6.0 33.0 4.0 36.0 44 21.96485349127182 26.0 7.0 33.0 4.0 36.0 45 24.195604738154614 29.0 14.0 34.0 4.0 37.0 46 24.1106608478803 30.0 4.0 35.0 4.0 38.0 47 23.468827930174562 29.0 4.0 35.0 4.0 38.0 48 23.41840710723192 29.0 4.0 35.0 4.0 38.0 49 21.05447319201995 25.0 4.0 34.0 4.0 38.0 50 23.20394326683292 29.0 4.0 35.0 4.0 38.0 51 24.520105985037407 31.0 4.0 37.0 4.0 39.0 52 24.317643391521198 31.0 4.0 37.0 4.0 39.0 53 23.950436408977556 31.0 4.0 36.0 4.0 39.0 54 23.16427680798005 30.0 4.0 36.0 4.0 39.0 55 23.37367518703242 30.0 4.0 36.0 4.0 39.0 56 22.91100374064838 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.1278814673947366 1 2 1.7012759905446941 1 3 1.841428582488362 1 4 2.3742391501294833 1 5 1.7960204698624054 1 6 2.2652656425818165 1 7 1.8232133580223575 1 8 2.4586765676423568 1 9 2.117165634965346 1 10 2.9720287942378647 1 11 -1.6587667435221753 1 12 -5.381524357994941 1 13 -17.22182450946916 1 14 -16.37995965922516 1 15 -11.654925842752721 1 16 -21.781002139792093 1 17 -20.721001748766298 1 18 -20.38181583465791 1 19 -6.124964561316066 1 20 -11.68967409254332 1 21 -5.963803842239745 1 22 -2.3549955531644997 1 23 -3.1667548648542905 1 24 -4.909452741773702 1 25 -2.997730789534657 1 26 2.948833658763327 1 27 1.9497084770278335 1 28 2.6367158154434165 1 29 0.7088561490607574 1 30 2.377027884525482 1 31 1.1146469581380032 1 32 -17.745972995077057 1 33 0.9236586990375457 1 34 -0.5516474502160129 1 35 1.1461025936209879 1 36 2.1500190362706384 1 37 2.774767508883553 1 38 0.6845506593020971 1 39 1.8230926727258314 1 40 5.687923557125114 1 41 -7.986615903278311 1 42 -12.197019540206897 1 43 -14.89111968605895 1 44 -15.551406356502252 1 45 -18.19732851375915 1 46 -17.887222930374303 1 47 -17.143296377801637 1 48 -17.318093156458715 1 49 -14.647421258852681 1 50 -16.949693643360522 1 51 -18.22820396530035 1 52 -18.021100177617924 1 53 -17.644459501377657 1 54 -17.005888561655023 1 55 -17.09636241946104 1 56 -16.9090139281068 2 1 1.1903814673947366 2 2 1.9176221443908474 2 3 1.7933516594114423 2 4 2.5136622270525635 2 5 2.0123666237085587 2 6 2.2508425656587363 2 7 2.8857133580223575 2 8 2.410599644565437 2 9 2.3623579426576526 2 10 3.000874948084018 2 11 -0.6779975127529454 2 12 -10.391139742610324 2 13 -10.630478355623008 2 14 -12.115536582302084 2 15 -6.145310458137338 2 16 -4.838694447484404 2 17 -1.432540210304758 2 18 -11.38181583465791 2 19 -1.1826568690083725 2 20 -4.256981784851014 2 21 3.6515807731448717 2 22 3.890196754527807 2 23 3.299591288991863 2 24 3.5376626428416813 2 25 4.002269210465343 2 26 4.2180644279940935 2 27 4.1564392462586 2 28 3.92998504621265 2 29 3.9828946105992173 2 30 3.9443355768331756 2 31 2.2444546504456966 2 32 -8.02481914892321 2 33 3.0198125451913924 2 34 2.7704679343993703 2 35 1.7759102859286813 2 36 5.212519036270638 2 37 6.890152124268166 2 38 7.848012197763637 2 39 9.909631134264291 2 40 12.601385095586654 2 41 8.051845635183227 2 42 5.952018921331565 2 43 0.35888031394105013 2 44 -6.738906356502252 2 45 2.9276714862408504 2 46 -11.574722930374303 2 47 -9.893296377801637 2 48 -9.255593156458715 2 49 -11.459921258852681 2 50 -8.074693643360522 2 51 -7.915703965300349 2 52 -8.146100177617924 2 53 -7.019459501377657 2 54 -11.443388561655023 2 55 -7.971362419461041 2 56 -8.471513928106798 3 1 1.8235336413077832 3 2 2.8116438835212847 3 3 2.787916876802747 3 4 3.364205705313431 3 5 2.9498666237085587 3 6 3.1883425656587363 3 7 3.301474227587576 3 8 3.1877735576089137 3 9 3.163988377440262 3 10 2.6910923393883692 3 11 3.311132922029664 3 12 3.2338602573896758 3 13 -0.8940653121447468 3 14 -1.6943409301281704 3 15 -2.3137887190069044 3 16 3.346088161211245 3 17 3.2957206592604606 3 18 3.139923295776871 3 19 0.8716909570785809 3 20 0.8354095194968139 3 21 4.434189468797044 3 22 4.4309576240930255 3 23 4.508830419426644 3 24 5.127336555885158 3 25 4.828356166987085 3 26 4.283281819298445 3 27 4.284156637562951 3 28 3.9109633070822127 3 29 4.344307654077475 3 30 3.8954225333549175 3 31 5.0243459547935245 3 32 3.752354764120266 3 33 5.248073414756611 3 34 4.659054890921112 3 35 4.838410285928681 3 36 7.9326277319228105 3 37 8.607543428615994 3 38 9.52736002385059 3 39 9.675935482090377 3 40 13.313341617325786 3 41 12.63880215692236 3 42 10.742779790896783 3 43 5.8480107487236594 3 44 4.4920719043673145 3 45 10.367888877545202 3 46 1.1997335913648257 3 47 -3.925905073453812 3 48 1.899298147889109 3 49 -8.47350821537442 3 50 1.745958530552521 3 51 1.3804916868735653 3 52 2.8919433006429465 3 53 2.2251057160136476 3 54 -0.2667581268724142 3 55 1.8166810587998299 3 56 7.221420854501897 4 1 1.1278814673947366 4 2 2.127849417118121 4 3 1.463806204865989 4 4 2.5875258634161966 4 5 1.9498666237085587 4 6 2.0065243838405564 4 7 1.414122448931451 4 8 2.1265087354745305 4 9 2.6521306699303793 4 10 2.807693129902198 4 11 4.299275214519781 4 12 3.2338602573896758 4 13 6.295658008013358 4 14 6.333327054061549 4 15 4.684234996408115 4 16 7.093123734333776 4 17 7.044732516967969 4 18 5.163638710796636 4 19 5.249161312809807 4 20 5.572563669694439 4 21 2.6515807731448717 4 22 3.6799694818005335 4 23 3.0780003799009563 4 24 3.656980824659861 4 25 3.2295419377380696 4 26 4.26351897344864 4 27 4.264393791713147 4 28 4.503848682576283 4 29 3.9885764287810375 4 30 1.2511537586513555 4 31 4.39786374135479 4 32 2.9638172147131563 4 33 3.815267090645939 4 34 3.378422479853917 4 35 1.383864831383228 4 36 4.7863826726342715 4 37 4.617424851540893 4 38 5.541194015945457 4 39 6.875540225173385 4 40 9.408203277404834 4 41 4.50639108972868 4 42 6.128155284967928 4 43 4.51797122303196 4 44 7.857684552588658 4 45 8.120853304422667 4 46 8.658231615080243 4 47 7.947612713107453 4 48 8.000088661723101 4 49 5.943487832056409 4 50 8.868488174821294 4 51 6.862705125608741 4 52 6.524354367836622 4 53 6.90099504407689 4 54 2.35774780198134 4 55 7.040001216902596 4 56 4.863713344620475 5 1 1.8897862292994958 5 2 2.9027411920098984 5 3 2.4897802308400117 5 4 3.4035431794335125 5 5 3.0927237665657046 5 6 3.2359616132777873 5 7 1.2517847865937881 5 8 3.1546472636130574 5 9 2.7581912759909883 5 10 2.316351138560208 5 11 3.238669153913719 5 12 7.043384066913486 5 13 8.027259739615086 5 14 7.7922015129360105 5 15 6.205880018053136 5 16 7.435115076325122 5 17 7.912697884933337 5 18 6.665803212961141 5 19 6.091152654801153 5 20 6.760875358006132 5 21 3.0325331540972513 5 22 3.095553897384953 5 23 3.4632817651823373 5 24 2.903734071413112 5 25 2.4308406390367736 5 26 1.4799691898988527 5 27 2.480844008163359 5 28 1.4389136176412194 5 29 2.503727943932553 5 30 2.3550498625474603 5 31 3.1402879837790323 5 32 4.814466565362505 5 33 2.293622069000918 5 34 2.356777458208896 5 35 2.076505524023922 5 36 4.435733321984923 5 37 4.009199743315786 5 38 4.508726483477922 5 39 4.284631134264291 5 40 4.559718428919986 5 41 3.8375599208975117 5 42 8.35975701656966 5 43 11.251737456798192 5 44 11.353355548259653 5 45 8.61219529576466 5 46 8.922300879149507 5 47 10.0947988602936 5 48 8.348573510207952 5 49 9.162102550671129 5 50 9.00268730902043 5 51 7.200367463271078 5 52 8.45509029857255 5 53 8.450778593860438 5 54 7.470301914535451 5 55 8.332209009110386 5 56 6.995747976655107 6 1 1.5088338483471162 6 2 2.8551221443908474 6 3 2.346923087982873 6 4 3.165447941338279 6 5 2.9022475760895077 6 6 3.1883425656587363 6 7 2.8232133580223575 6 8 3.2022663112321013 6 9 2.3296198474195577 6 10 3.221113043322113 6 11 3.5720024872470546 6 12 6.281479305008727 6 13 6.503450215805561 6 14 7.696963417697916 6 15 5.729689541862662 6 16 6.863686504896545 6 17 7.484126456361906 6 18 6.189612736770659 6 19 6.281628845277343 6 20 6.760875358006132 6 21 5.223009344573441 6 22 5.476506278337332 6 23 4.225186527087104 6 24 4.570400738079776 6 25 4.4784596866558175 6 26 3.5752072851369476 6 27 4.0998916272109796 6 28 3.2960564747840806 6 29 4.884680324884933 6 30 1.0693355768331756 6 31 3.9974308409218864 6 32 6.385895136791074 6 33 4.103145878524728 6 34 5.118682220113655 6 35 4.933648381166776 6 36 5.102399988651587 6 37 3.1996759337919762 6 38 5.318250293001732 6 39 6.856059705692861 6 40 3.274004143205701 6 41 3.3613694447070372 6 42 5.883566540379185 6 43 11.394594599655335 6 44 9.877165072069175 6 45 7.755052438621803 6 46 11.255634212482843 6 47 11.618608384103123 6 48 11.539049700684139 6 49 12.685912074480651 6 50 11.526496832829952 6 51 10.962272225175841 6 52 11.550328393810645 6 53 11.117445260527102 6 54 10.232206676440214 6 55 10.141732818634196 6 56 10.376700357607486 7 1 1.842167181680452 7 2 2.6646459539146576 7 3 2.108827849887632 7 4 3.4511622270525635 7 5 2.9022475760895077 7 6 3.1407235180396853 7 7 2.8232133580223575 7 8 3.2498853588511523 7 9 3.186762704562412 7 10 2.935398757607828 7 11 4.90533582058039 7 12 6.5671935907230115 7 13 8.170116882472232 7 14 8.887439608174105 7 15 7.967784779957903 7 16 8.435115076325122 7 17 7.865078837314293 7 18 7.570565117723039 7 19 5.710200273848773 7 20 6.0465896437204165 7 21 3.9372950588591564 7 22 4.762220564051617 7 23 4.891853193753768 7 24 5.046591214270251 7 25 4.002269210465343 7 26 1.194254904184568 7 27 2.52846305578241 7 28 4.010342189069796 7 29 3.360870801075407 7 30 4.259811767309365 7 31 3.426002269493317 7 32 5.33827608917203 7 33 5.531717307096152 7 34 5.737729839161275 7 35 5.743172190690586 7 36 2.530971417223018 7 37 2.8187235528395966 7 38 2.4611074358588745 7 39 5.713202562835722 7 40 -5.916472047270489 7 41 -8.067201983864392 7 42 6.835947492760138 7 43 10.870785075845813 7 44 9.829546024450128 7 45 7.231242914812277 7 46 10.731824688673317 7 47 11.285275050769794 7 48 11.300954462588905 7 49 13.257340645909224 7 50 11.907449213782332 7 51 13.295605558509177 7 52 14.026518870001127 7 53 13.117445260527102 7 54 13.470301914535451 7 55 13.665542342443718 7 56 12.995747976655107 8 1 1.4808226438653236 8 2 2.5610044973320214 8 3 0.0948222476467393 8 4 3.0393975211702084 8 5 2.7733960354732616 8 6 3.0118719774234393 8 7 2.9408604168458865 8 8 2.653246703388966 8 9 2.8366226485400077 8 10 3.125874948084018 8 11 4.866120134305881 8 12 7.351507316213205 8 13 7.6967275267299335 8 14 8.991081064756742 8 15 7.729689541862662 8 16 7.9701290819273645 8 17 7.699812730871713 8 18 7.029948871224445 8 19 5.6849901898151565 8 20 6.500371156325457 8 21 4.533933714321343 8 22 4.658579107468981 8 23 5.16356187722716 8 24 5.239868525194623 8 25 4.943445681053575 8 26 3.8357114868176225 8 27 4.248351010964484 8 28 5.043955634447947 8 29 2.5233357870698043 8 30 4.481100282715531 8 31 2.3951899445633416 8 32 4.688416145194434 8 33 3.7109890157796244 8 34 3.2447326402817254 8 35 3.8972338153404493 8 36 2.3264896245059354 8 37 1.91956388897405 8 38 4.281835727175405 8 39 3.9316899577937043 8 40 -0.7147913750015817 8 41 -11.03638965893442 8 42 6.440989509566858 8 43 10.638292078646934 8 44 7.978005408203632 8 45 7.449730309770263 8 46 8.348071187272755 8 47 11.03317421043366 8 48 9.564259784717756 8 49 13.764343447029674 8 50 9.81501223899242 8 51 12.183560740582006 8 52 12.625958645911489 8 53 13.296716969210575 8 54 11.52352320305086 8 55 13.550696404068372 8 56 12.561574307187318 9 1 1.5167703562836223 9 2 1.132899922168626 9 3 1.9183516594114423 9 4 2.340051115941449 9 5 2.3943110681530015 9 6 2.9105647878809577 9 7 2.434324469133472 9 8 2.7022663112321013 9 9 2.702635720435431 9 10 3.3480971703062394 9 11 2.9053358205803903 9 12 5.011638035167454 9 13 6.598688311043656 9 14 7.474741195475694 9 15 6.118578430751548 9 16 6.855749996960039 9 17 6.428570900806356 9 18 5.729295276453204 9 19 4.178454242102742 9 20 6.28468488181565 9 21 4.262691884255986 9 22 3.952696754527807 9 23 3.598202400102977 9 24 5.308495976175017 9 25 3.946713654909786 9 26 4.606953316882979 9 27 4.274494801814157 9 28 3.1452628239904286 9 29 4.170394610599217 9 30 2.847113354610954 9 31 2.6402879837790323 9 32 3.1001808510767894 9 33 1.7142569896358353 9 34 0.8885234899549275 9 35 2.0606325081509027 9 36 1.7055745918261955 9 37 1.6679299020459446 9 38 0.11190108665252652 9 39 2.729075578708734 9 40 1.1708295400310966 9 41 -2.892598809261216 9 42 6.820074476887122 9 43 10.386658091718829 9 44 10.337482532386638 9 45 6.80267148624085 9 46 9.168332625181254 9 47 9.690036955531696 9 48 8.959684621319063 9 49 11.297023185591762 9 50 10.216973023306146 9 51 9.771796034699651 9 52 9.756677600159854 9 53 10.577762720844564 9 54 8.494111438344977 9 55 9.62585980276118 9 56 9.479874960782091 10 1 1.1867049968065047 10 2 2.6786515561555504 10 3 1.7418810711761452 10 4 3.0982210505819765 10 5 2.6557489766497326 10 6 2.6001072715410913 10 7 2.4114486521400025 10 8 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-5.369524925291387 114 55 -1.9145442376428576 114 56 1.6364406173477484 115 1 1.2389925785058509 115 2 1.9662332555019617 115 3 1.807240548300328 115 4 2.5622733381636777 115 5 2.060977734819673 115 6 2.1883425656587363 115 7 2.489880024689022 115 8 2.535599644565437 115 9 2.4248579426576526 115 10 2.3480971703062394 115 11 0.34978026502483317 115 12 -4.099473075943656 115 13 -1.29020057784523 115 14 -5.080814360079863 115 15 -1.4925326803595595 115 16 -7.310916669706625 115 17 -5.849206876971426 115 18 -2.2707047235467996 115 19 -2.654879091230594 115 20 -3.381981784851014 115 21 0.20713632870042886 115 22 1.3971411989722498 115 23 1.2093135112140843 115 24 1.364051531730567 115 25 0.5578247660209001 115 26 2.829175539105208 115 27 3.4967170240363785 115 28 4.645262823990429 115 29 4.281505721710332 115 30 4.069335576833176 115 31 4.695843539334582 115 32 -6.344263593367653 115 33 3.992034767413614 115 34 4.610745712177149 115 35 4.61618806370646 115 36 5.816685702937303 115 37 4.279041013157052 115 38 7.3341233088747515 115 39 8.951297800930956 115 40 12.226385095586654 115 41 7.107401190738784 115 42 2.042296699109343 115 43 -0.33556413050339273 115 44 -1.884739689835584 115 45 2.8026714862408504 115 46 -2.7761118192631926 115 47 -3.6988519333571936 115 48 -5.651426489792049 115 49 -10.091865703297126 115 50 -1.1719158655827435 115 51 5.327351590255208 115 52 5.756677600159854 115 53 2.5777627208445644 115 54 4.216333660567198 115 55 5.12585980276118 115 56 5.646541627448759 116 1 1.7112148007280723 116 2 2.438455477724183 116 3 2.501684992744778 116 4 3.034495560385899 116 5 2.5331999570418944 116 6 2.771675898992072 116 7 2.906546691355693 116 8 2.7022663112321013 116 9 2.591524609324317 116 10 3.0425416147506823 116 11 0.6553358205803903 116 12 0.9838602573896758 116 13 -12.65131168895634 116 14 -10.386369915635417 116 15 -0.5203104581373381 116 16 -7.588694447484404 116 17 -8.349206876971426 116 18 -1.8818158346579104 116 19 -2.9326568690083725 116 20 -2.9653151181843462 116 21 4.151580773144872 116 22 4.619363421194471 116 23 4.487091288991863 116 24 4.808495976175017 116 25 3.9189358771320073 116 26 4.2180644279940935 116 27 4.7189392462586 116 28 4.36748504621265 116 29 4.753727943932553 116 30 4.819335576833176 116 31 3.223621317112361 116 32 -4.316485815589878 116 33 5.186479211858057 116 34 4.499634601066035 116 35 3.8384102859286813 116 36 7.066685702937303 116 37 7.390152124268166 116 38 6.306345531096973 116 39 9.867964467597627 116 40 11.976385095586654 116 41 8.801845635183227 116 42 7.097852254664897 116 43 -11.39111968605895 116 44 2.615260310164416 116 45 8.80267148624085 116 46 2.3627770696256967 116 47 -4.226629711134969 116 48 -3.9014264897920476 116 49 -0.48075459218601324 116 50 3.050306356639478 116 51 9.605129368032983 116 52 8.562233155715408 116 53 6.272207165289011 116 54 4.410778105011644 116 55 5.236970913872291 116 56 7.674319405226534 117 1 -0.1578328183195481 117 2 0.28369357296227804 117 3 0.6326373736971576 117 4 1.1654479413382788 117 5 0.664152337994274 117 6 0.6169139942301669 117 7 1.2517847865937881 117 8 1.2498853588511523 117 9 1.139143656943368 117 10 1.125874948084018 117 11 -15.71371179846723 117 12 -17.051854028324613 117 13 -11.782264069908724 117 14 -12.160179439444942 117 15 -14.698881886708767 117 16 -12.802980161770119 117 17 -14.3253973531619 117 18 -4.096101548943626 117 19 -2.146942583294088 117 20 -2.381981784851014 117 21 1.365866487430587 117 22 0.3812681830992375 117 23 1.1299484318490087 117 24 0.9037340714131119 117 25 0.1451263533224889 117 26 1.4323501422798088 117 27 2.4332249605443153 117 28 3.1531993319269347 117 29 3.0275374677420714 117 30 3.640764148261745 117 31 3.235526079017127 117 32 -4.4712477203517835 117 33 1.3412411166199618 117 34 3.6901107915422244 117 35 2.5526960002143966 117 36 5.150019036270638 117 37 5.533009267125312 117 38 5.794440769192207 117 39 6.141773991407145 117 40 8.654956524158084 117 41 0.8375599208975117 117 42 2.407376064188707 117 43 -5.462548257487521 117 44 -8.97997778507368 117 45 -0.34018565661629196 117 46 2.255634212482839 117 47 1.1424179079126482 117 48 -4.8895217278872884 117 49 3.4954358840044613 117 50 2.621734928068051 117 51 -0.7996325367289217 117 52 -1.0211001776179245 117 53 -0.7873166442347994 117 54 -5.720174275940737 117 55 -4.524933848032468 117 56 -4.766156785249656 118 1 0.4135957531090213 118 2 0.28369357296227804 118 3 1.7754945165542964 118 4 0.8797336556239941 118 5 0.664152337994274 118 6 1.616913994230167 118 7 0.9660705008795034 118 8 1.6784567874225829 118 9 1.8534293712290832 118 10 2.4115892337983027 118 11 -7.71371179846723 118 12 -6.623282599753182 118 13 -7.210835498480151 118 14 2.2683919891264885 118 15 -15.41316760099448 118 16 -2.802980161770119 118 17 -13.611111638876185 118 18 -0.5246729775150527 118 19 -0.8612282975797996 118 20 -1.2391246419938717 118 21 2.223009344573441 118 22 3.809839611670661 118 23 2.4156627175632934 118 24 -4.524837357158319 118 25 1.4308406390367736 118 26 2.146635856565524 118 27 1.4332249605443153 118 28 1.5817707604983653 118 29 2.7418231820277867 118 30 2.640764148261745 118 31 1.2355260790171272 118 32 -14.756962006066068 118 33 1.6269554023342465 118 34 1.261539362970801 118 35 -0.5901611426427458 118 36 4.864304750556354 118 37 4.818723552839597 118 38 5.794440769192207 118 39 7.570345419978576 118 40 11.3692422384438 118 41 7.551845635183227 118 42 0.5502332070458493 118 43 -0.8911196860589499 118 44 -2.265692070787967 118 45 6.088385771955135 118 46 3.255634212482839 118 47 -8.714724949230208 118 48 -2.460950299315858 118 49 -4.361706973138395 118 50 1.050306356639478 118 51 1.2003674632710783 118 52 0.40747125095350256 118 53 0.9269690700509159 118 54 -7.0058885616550235 118 55 -1.3820767051753258 118 56 4.662414643321775 119 1 1.8778814673947366 119 2 2.6051221443908474 119 3 2.5433516594114423 119 4 3.2011622270525635 119 5 2.6998666237085587 119 6 2.3133425656587363 119 7 2.6982133580223575 119 8 2.660599644565437 119 9 2.5498579426576526 119 10 2.375874948084018 119 11 -10.552997512752945 119 12 -9.016139742610324 119 13 -0.5679783556230085 119 14 -1.4280365823020844 119 15 -4.770310458137338 119 16 -9.338694447484404 119 17 -2.432540210304758 119 18 1.7431841653420896 119 19 0.5673431309916275 119 20 0.7430182151489859 119 21 2.5265807731448717 119 22 3.077696754527807 119 23 3.362091288991863 119 24 -4.024837357158319 119 25 2.752269210465343 119 26 4.2180644279940935 119 27 4.0939392462586 119 28 3.86748504621265 119 29 4.045394610599217 119 30 3.9443355768331756 119 31 2.0569546504456966 119 32 -9.27481914892321 119 33 3.1448125451913924 119 34 2.8329679343993703 119 35 0.3384102859286813 119 36 3.1500190362706384 119 37 3.390152124268166 119 38 4.098012197763637 119 39 6.159631134264291 119 40 8.226385095586654 119 41 5.926845635183227 119 42 5.639518921331565 119 43 -5.01611968605895 119 44 -2.926406356502252 119 45 -0.8223285137591496 119 46 -9.887222930374303 119 47 -8.643296377801637 119 48 -14.193093156458715 119 49 -9.147421258852681 119 50 -12.949693643360522 119 51 -11.853203965300349 119 52 -11.896100177617924 119 53 -14.019459501377657 119 54 -12.255888561655023 119 55 -13.971362419461041 119 56 -11.034013928106798 120 1 0.2187905583038301 120 2 1.0369403262090273 120 3 0.9183516594114423 120 4 1.6329804088707434 120 5 1.1316848055267386 120 6 1.3701607474769162 120 7 1.2777588125678108 120 8 1.535599644565437 120 9 1.4248579426576526 120 10 2.2167840389931115 120 11 -12.337088421843855 120 12 -3.2206851971557775 120 13 -9.886160173804825 120 14 -4.757582036847538 120 15 -6.5430377308646115 120 16 -7.361421720211677 120 17 -12.455267483032031 120 18 -3.4727249255670003 120 19 -3.4326568690083725 120 20 -4.5637999666691975 120 21 -1.2575101359460348 120 22 0.13451493634598677 120 23 0.07800037990095632 120 24 -2.979382811703772 120 25 0.002269210465343008 120 26 -0.10011739018772658 120 27 2.6280301553495065 120 28 2.503848682576283 120 29 1.9885764287810375 120 30 -0.20339169589409778 120 31 -1.4657726222815768 120 32 -7.354364603468664 120 33 -1.9574601820813342 120 34 -8.348850247418813 120 35 0.20204664956504814 120 36 0.7863826726342751 120 37 -2.064393330277287 120 38 0.8593758341272739 120 39 3.7391765888097446 120 40 -0.319069449867893 120 41 2.642754726092317 120 42 -4.371844715032072 120 43 -1.9820287769680398 120 44 -4.824133629229525 120 45 -9.106419422850058 120 46 -12.887222930374303 120 47 -12.41602365052891 120 48 -13.227184065549624 120 49 -14.011057622489044 120 50 -12.131511825178704 120 51 -12.773658510754894 120 52 -13.202918359436106 120 53 -13.189914046832202 120 54 -10.551343107109568 120 55 -11.641816964915586 120 56 -10.363559382652253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 277.0 5 41.0 6 10.0 7 26.0 8 61.0 9 38.0 10 22.0 11 28.0 12 26.0 13 31.0 14 39.0 15 67.0 16 63.0 17 94.0 18 123.0 19 137.0 20 169.0 21 236.0 22 251.0 23 311.0 24 323.0 25 372.0 26 427.0 27 413.0 28 394.0 29 448.0 30 512.0 31 607.0 32 769.0 33 868.0 34 1132.0 35 1242.0 36 1384.0 37 1116.0 38 615.0 39 160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 97.1399625935162 1.30143391521197 0.7559226932668329 0.8026807980049875 8 98.80766832917706 0.8338528678304239 0.12468827930174563 0.23379052369077308 9 97.201652506041 1.1848156520383506 0.8574323797645957 0.7560994621560526 10 60.33354114713217 21.921758104738153 10.715399002493765 7.029301745635911 11 41.980985037406484 26.03647132169576 14.097568578553615 17.88497506234414 12 34.943890274314214 12.749376558603492 23.285536159600998 29.021197007481298 13 25.87281795511222 15.827618453865336 24.04145885286783 34.258104738154614 14 22.760932262841997 15.932652583989398 26.494660534726012 34.81175461844259 15 22.496292248848647 17.742564983217548 26.703614081648585 33.057528686285224 16 27.81415653258497 19.332709697536636 25.085749922045526 27.767383847832868 17 29.420018709073904 21.289367009666353 22.372934206423448 26.917680074836298 18 30.58997739848804 23.910840932117527 23.131478450627387 22.367703218767048 19 30.40530007794232 26.492595479345283 19.890880748246296 23.211223694466096 20 28.746296585061593 26.937470762513644 19.858100732886324 24.458131919538438 21 28.238503507404523 24.668745128604833 22.79812938425565 24.294621979734995 22 27.534876471046683 24.58888629101395 21.876704855428258 25.999532382511102 23 28.966645885286784 26.55081047381546 23.25436408977556 21.228179551122196 24 26.90149625935162 27.899002493765586 19.97350374064838 25.225997506234414 25 25.623441396508728 29.013403990024937 19.841022443890274 25.52213216957606 26 26.774420238931835 25.993597251503086 21.589755602404935 25.642226907160147 27 25.325536062378166 23.22027290448343 23.34502923976608 28.109161793372316 28 22.65417642466823 29.281811085089775 23.42701014832162 24.637002341920375 29 24.148413750097436 27.640501987684154 24.966871930781824 23.24421233143659 30 22.403306300686214 26.762320648783533 27.206799750467876 23.627573300062384 31 25.320212433614497 24.58606685410809 29.2174320524836 20.876288659793815 32 27.786438035853468 20.849571317225255 27.770849571317225 23.593141075604056 33 29.983602717263995 22.651674865308035 27.06332474428047 20.3013976731475 34 24.214057258756533 25.064357594196114 30.51720102972151 20.204384117325844 35 23.425319613345806 27.58808855628313 29.817586529466794 19.16900530090427 36 24.083034181364134 29.514593413454033 27.368503199625412 19.03386920555642 37 26.03467124785257 27.369982820552867 28.049351866312666 18.5459940652819 38 25.495706479313036 27.806401249024198 27.650273224043715 19.047619047619047 39 22.470680218921032 22.267396403440188 24.49569976544175 30.76622361219703 40 14.511708042916439 15.7412483358133 20.667240974234474 49.07980264703579 41 16.17947509036618 18.568285399968566 23.110168159673112 42.14207134999214 42 21.786356177229038 26.185824802688128 29.092756114714387 22.935062905368444 43 13.680832746341082 14.674806292556939 23.354465054394616 48.289895906707365 44 14.940098661028895 16.169446402004542 22.33184558765954 46.55860934930702 45 22.64607223652391 24.931741945549575 29.089632576644043 23.332553241282472 46 21.17334997659541 24.988297706350444 29.872054922764864 23.96629739428928 47 19.323407903967574 23.805440798191597 27.562553589523738 29.308597708317098 48 22.427502338634238 26.223885251013407 32.40567508574993 18.942937324602433 49 15.482054890921887 16.58456486042693 22.9650480882008 44.96833216045039 50 21.208808371076056 25.1132281742933 33.46087771357176 20.21708574105888 51 23.172543194433587 25.76811820811508 29.661480728637322 21.39785786881401 52 21.205007824726135 26.877934272300468 30.962441314553992 20.954616588419405 53 20.604309806371017 28.013741411617737 29.723610243597754 21.658338538413492 54 18.400312134217714 23.20717908700741 28.981662114709323 29.41084666406555 55 21.52392848778203 24.841908033414008 31.509095167460377 22.125068311343586 56 21.669529907855694 26.175230360768392 29.462751835077306 22.692487896298612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.0 9 4.0 10 3.0 11 2.0 12 4.0 13 9.5 14 13.0 15 17.5 16 22.0 17 39.5 18 57.0 19 88.5 20 120.0 21 105.5 22 90.0 23 89.0 24 106.5 25 124.0 26 130.5 27 137.0 28 168.5 29 241.5 30 283.0 31 290.0 32 297.0 33 315.5 34 334.0 35 345.5 36 357.0 37 304.5 38 246.5 39 241.0 40 228.0 41 215.0 42 206.0 43 197.0 44 193.5 45 190.0 46 193.5 47 225.0 48 253.0 49 292.5 50 332.0 51 347.0 52 362.0 53 396.0 54 450.5 55 471.0 56 520.5 57 570.0 58 610.0 59 650.0 60 696.0 61 742.0 62 844.0 63 995.5 64 1045.0 65 968.5 66 892.0 67 811.5 68 731.0 69 683.5 70 636.0 71 537.5 72 375.0 73 311.0 74 271.5 75 232.0 76 199.0 77 166.0 78 125.5 79 72.5 80 60.0 81 44.0 82 28.0 83 17.5 84 7.0 85 3.5 86 0.0 87 0.0 88 1.0 89 2.0 90 2.0 91 2.0 92 1.5 93 1.0 94 2.0 95 3.0 96 5.5 97 4.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.023379052369077308 10 0.0 11 0.0 12 0.0 13 0.0 14 0.023379052369077308 15 0.16365336658354115 16 0.031172069825436407 17 0.031172069825436407 18 0.007793017456359102 19 0.015586034912718203 20 0.046758104738154616 21 0.015586034912718203 22 0.007793017456359102 23 0.0 24 0.0 25 0.0 26 0.19482543640897756 27 0.05455112219451371 28 0.17144638403990023 29 0.023379052369077308 30 0.062344139650872814 31 0.21820448877805484 32 0.015586034912718203 33 0.19482543640897756 34 0.10130922693266833 35 0.031172069825436407 36 0.14027431421446385 37 0.20261845386533667 38 0.17144638403990023 39 0.3273067331670823 40 0.4909600997506235 41 0.8260598503740649 42 0.2727556109725686 43 0.42861596009975067 44 0.47537406483790523 45 0.10130922693266833 46 0.10910224438902742 47 0.023379052369077308 48 0.031172069825436407 49 0.3350997506234414 50 0.20261845386533667 51 0.3195137157107232 52 0.40523690773067333 53 0.18703241895261846 54 0.13248129675810474 55 0.17923940149625936 56 0.20261845386533667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 12832.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.60941396508728 #Duplication Level Percentage of deduplicated Percentage of total 1 94.40096327513547 85.53615960099751 2 3.595080416272469 6.514962593516209 3 1.1180872108024427 3.03927680798005 4 0.34402683409305923 1.2468827930174564 5 0.16341274619420315 0.7403366583541148 6 0.1462114044895502 0.7948877805486284 7 0.043003354261632404 0.2727556109725686 8 0.05160402511395889 0.3740648379052369 9 0.017201341704652963 0.14027431421446385 >10 0.12040939193257075 1.3403990024937655 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 16 0.12468827930174563 No Hit ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 16 0.12468827930174563 No Hit ACACCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 15 0.11689526184538654 No Hit ACACCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCCCGCAGCATTTGATATG 14 0.10910224438902742 No Hit ACACCCGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA 13 0.10130922693266833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.03117206982543641 0.0 0.0 0.0 0.0 14 0.03117206982543641 0.0 0.0 0.0 0.0 15 0.03896508728179551 0.0 0.0 0.0 0.0 16 0.046758104738154616 0.0 0.0 0.0 0.0 17 0.05455112219451372 0.0 0.0 0.0 0.0 18 0.05455112219451372 0.0 0.0 0.0 0.0 19 0.05455112219451372 0.0 0.0 0.0 0.0 20 0.05455112219451372 0.0 0.0 0.0 0.0 21 0.05455112219451372 0.0 0.0 0.0 0.0 22 0.05455112219451372 0.0 0.0 0.0 0.0 23 0.06234413965087282 0.0 0.0 0.0 0.0 24 0.06234413965087282 0.0 0.0 0.0 0.0 25 0.07013715710723192 0.0 0.0 0.0 0.0 26 0.07013715710723192 0.0 0.0 0.0 0.0 27 0.07013715710723192 0.0 0.0 0.0 0.0 28 0.08572319201995013 0.0 0.0 0.0 0.0 29 0.08572319201995013 0.0 0.0 0.0 0.0 30 0.08572319201995013 0.0 0.0 0.0 0.0 31 0.08572319201995013 0.0 0.0 0.0 0.0 32 0.08572319201995013 0.0 0.0 0.0 0.0 33 0.08572319201995013 0.0 0.0 0.0 0.0 34 0.08572319201995013 0.0 0.0 0.0 0.0 35 0.08572319201995013 0.0 0.0 0.0 0.0 36 0.08572319201995013 0.0 0.0 0.0 0.0 37 0.09351620947630923 0.0 0.0 0.0 0.0 38 0.09351620947630923 0.0 0.0 0.0 0.0 39 0.09351620947630923 0.0 0.0 0.0 0.0 40 0.09351620947630923 0.0 0.0 0.0 0.0 41 0.10130922693266833 0.0 0.0 0.0 0.0 42 0.10130922693266833 0.0 0.0 0.0 0.0 43 0.10130922693266833 0.0 0.0 0.0 0.0 44 0.10130922693266833 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACAAA 20 3.6427594E-4 51.020164 40 ACAAACC 15 0.007604789 51.02016 38 CAAACCA 15 0.007604789 51.02016 39 TTGATAC 20 3.9445664E-4 50.21032 33 CAGCAAC 20 4.023036E-4 50.01186 21 CAACATC 20 4.023036E-4 50.01186 24 GCAACAT 20 4.023036E-4 50.01186 23 TCAGCAA 20 4.023036E-4 50.01186 20 AGCAACA 20 4.023036E-4 50.01186 22 CTTTTGA 15 0.008233055 50.011856 30 ATCCTGC 15 0.008233055 50.011856 20 TTTTGAT 15 0.008233055 50.011856 31 ATCTTTT 15 0.008233055 50.011856 28 CCTGCCA 15 0.008233055 50.011856 22 GCGGTTA 15 0.008233055 50.011856 30 ACATCTT 15 0.008233055 50.011856 26 AGCGGTT 15 0.008233055 50.011856 29 TCTTTTG 15 0.008233055 50.011856 29 CGGTTAA 15 0.008233055 50.011856 31 TCCTGCC 15 0.008233055 50.011856 21 >>END_MODULE