FastQCFastQC Report
Fri 17 Jun 2016
SRR1526332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199022
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG11550.580337852096753No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG9760.4903980464471265No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG8890.44668428615931904No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG6770.34016339902121373No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG6150.30901106410346596No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG5070.25474570650480854No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2960.14872727638150557No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2800.14068796414466742No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG2390.1200872265377697No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG2290.11506265638974586No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2230.11204791430093156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGGA150.00809792550.49681541
AACTACT150.00820455150.3300336
TATGCAC204.0471004E-450.31724535
ACTATAC204.108359E-450.16434542
TCATACC204.108359E-450.16434543
ACGATTC1350.050.13895448
ACGATTA2400.050.1389548
CATAACG150.00832887550.1389544
CACGATT1200.050.12626647
AACGATT2650.050.12626647
TCGATGA150.00837899550.06292750
ATTATAG150.00845459549.94931832
GACGAGT150.00846302649.93672631
TCGCATA204.2068568E-449.92414530
ATTAGTT150.00847146349.9241429
CGGATAT150.00848835749.89899413
ACGCGTA150.00848835749.89899415
CAGACGT150.0085222249.84876623
ATATGCC2700.049.26032334
CATATGC9650.049.2598533
Produced by FastQC (version 0.11.5)