FastQCFastQC Report
Fri 17 Jun 2016
SRR1526331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207018
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG24721.1940990638495204No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG21291.0284129882425683No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG19130.9240742350906683No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG12920.6241003197789564No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG12720.6144393241167435No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG8800.42508380913736965No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG8680.41928721174004197No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG6810.3289569022983509No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG4980.24055879198910238No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG4750.22944864697755749No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC3710.17921146953405018No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG3430.16568607560695206No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG3390.16375387647450945No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG3380.1632708266913988No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGACACACGCAGGGATGAG3060.14781323363185808No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3020.1458810344994155No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCAGTCTCAAAGATTAAG2770.13380478992164935No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG2760.13332174013853867No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG2650.12800819252432155No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2530.12221159512699378No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAACGATTAAG2220.1072370518505637No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG2220.1072370518505637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACGT150.008166715550.38963737
TCGCCGA355.044967E-850.2667636
GTCGACG204.077394E-450.24226435
TCGAATC150.00826184250.2422643
TGGTAGC150.00826184250.2422635
CGATACC150.00828575250.20555544
ATATGCG204.121733E-450.13229434
TAGGATT252.049165E-550.13229434
ATACGCT204.121733E-450.13229434
CGATTTA204.1266833E-450.1201149
ACGATTT204.131638E-450.10792548
ACGGATG2000.050.1079248
ATTACAG150.0083577950.0957545
CCACGAT2400.050.0957546
CAACGAT5900.050.09574546
AACGTTT150.00836582350.08357647
CACGATT2450.050.08357647
GGATTAG150.00838995650.047150
GATTAAT355.2221367E-850.04709650
CCCTATG150.00847077549.9258932
Produced by FastQC (version 0.11.5)